Incidental Mutation 'R3749:Wnt2'
ID 310090
Institutional Source Beutler Lab
Gene Symbol Wnt2
Ensembl Gene ENSMUSG00000010797
Gene Name wingless-type MMTV integration site family, member 2
Synonyms Mirp, 2610510E18Rik, Int1l1, m-irp, Irp, Wnt2a, Wnt-2
MMRRC Submission 040734-MU
Accession Numbers
Essential gene? Possibly essential (E-score: 0.531) question?
Stock # R3749 (G1)
Quality Score 225
Status Validated
Chromosome 6
Chromosomal Location 17988939-18030584 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 18023167 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Valine at position 161 (I161V)
Ref Sequence ENSEMBL: ENSMUSP00000010941 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000010941]
AlphaFold P21552
Predicted Effect probably benign
Transcript: ENSMUST00000010941
AA Change: I161V

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000010941
Gene: ENSMUSG00000010797
AA Change: I161V

DomainStartEndE-ValueType
signal peptide 1 25 N/A INTRINSIC
WNT1 43 349 5.1e-213 SMART
Meta Mutation Damage Score 0.0968 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.2%
Validation Efficiency 98% (50/51)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutants exhibit impaired placental vascularization, runting and 50% perinatal mortality resulting from difficulties in breathing and nursing. Survivors of the perinatal period tend to recover, and adults are healthy and fertile. [provided by MGI curators]
Allele List at MGI

All alleles(4) : Targeted, knock-out(1) Targeted, other(3)
Other mutations in this stock
Total: 51 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam12 T C 7: 133,774,594 (GRCm39) D5G probably damaging Het
Adam19 A T 11: 46,028,437 (GRCm39) D690V probably benign Het
Ankib1 G T 5: 3,784,097 (GRCm39) P293Q probably damaging Het
Arhgap19 T A 19: 41,762,518 (GRCm39) E461V probably damaging Het
Bckdk C A 7: 127,504,590 (GRCm39) R105S probably damaging Het
Bub1b T A 2: 118,445,936 (GRCm39) N319K possibly damaging Het
Ccdc40 T C 11: 119,155,252 (GRCm39) V1164A probably benign Het
Cfhr1 C A 1: 139,485,372 (GRCm39) probably null Het
Colq A T 14: 31,271,410 (GRCm39) probably benign Het
Daam1 C A 12: 72,017,940 (GRCm39) D716E probably damaging Het
Dnah17 T C 11: 117,973,742 (GRCm39) S1935G probably benign Het
Dnah8 A T 17: 31,003,148 (GRCm39) K3616* probably null Het
Faap24 A T 7: 35,092,437 (GRCm39) V160D possibly damaging Het
Gpc1 C A 1: 92,785,304 (GRCm39) C414* probably null Het
Igkv9-120 G A 6: 68,026,985 (GRCm39) A7T probably benign Het
Kcnu1 T A 8: 26,376,798 (GRCm39) C391S probably null Het
Lrba T G 3: 86,283,260 (GRCm39) L1858R probably damaging Het
Lrp10 A T 14: 54,706,723 (GRCm39) N520I possibly damaging Het
Marchf6 A G 15: 31,462,160 (GRCm39) V856A probably benign Het
Mfsd1 T C 3: 67,490,286 (GRCm39) S46P probably benign Het
Mme T A 3: 63,250,961 (GRCm39) V334E probably damaging Het
Mroh2b G A 15: 4,981,728 (GRCm39) W1513* probably null Het
Nuf2 T A 1: 169,352,945 (GRCm39) N20I probably damaging Het
Obsl1 T C 1: 75,474,890 (GRCm39) T642A probably benign Het
Olfm1 C T 2: 28,098,100 (GRCm39) T54I probably damaging Het
Or10c1 G A 17: 37,522,691 (GRCm39) R18C possibly damaging Het
Or6c3b C T 10: 129,527,830 (GRCm39) V27I probably benign Het
Osbpl7 T C 11: 96,946,879 (GRCm39) V223A probably damaging Het
Patj A G 4: 98,357,837 (GRCm39) Y701C probably damaging Het
Pkdrej T A 15: 85,705,278 (GRCm39) K219N probably damaging Het
Scn2a T G 2: 65,544,115 (GRCm39) V832G probably damaging Het
Secisbp2l C T 2: 125,582,657 (GRCm39) G933D possibly damaging Het
Slc25a25 G T 2: 32,310,392 (GRCm39) N122K probably benign Het
Slk T G 19: 47,608,248 (GRCm39) D400E possibly damaging Het
Spon1 T C 7: 113,365,621 (GRCm39) L19P probably damaging Het
Spon1 T A 7: 113,616,024 (GRCm39) V297E possibly damaging Het
Supt16 A G 14: 52,417,596 (GRCm39) L306P probably damaging Het
Syne1 C T 10: 5,002,267 (GRCm39) probably benign Het
Tas2r136 A T 6: 132,754,200 (GRCm39) F309Y probably damaging Het
Terb1 T C 8: 105,223,466 (GRCm39) D114G probably damaging Het
Tlr5 T C 1: 182,802,004 (GRCm39) I436T probably benign Het
Tpcn2 T C 7: 144,809,260 (GRCm39) H682R probably damaging Het
Tpi1 T A 6: 124,789,754 (GRCm39) S130C probably damaging Het
Ttn G A 2: 76,584,350 (GRCm39) H22253Y probably damaging Het
Uggt2 A T 14: 119,295,084 (GRCm39) V38E probably benign Het
Vmn1r158 A C 7: 22,489,639 (GRCm39) L190W probably damaging Het
Vwa5b2 A G 16: 20,417,076 (GRCm39) probably benign Het
Zdhhc25 C T 15: 88,485,226 (GRCm39) S187L probably benign Het
Zfhx4 G A 3: 5,308,225 (GRCm39) E484K possibly damaging Het
Zkscan1 T C 5: 138,099,703 (GRCm39) S476P probably damaging Het
Zmynd8 A G 2: 165,647,118 (GRCm39) Y945H probably damaging Het
Other mutations in Wnt2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03264:Wnt2 APN 6 17,989,959 (GRCm39) missense probably benign 0.22
1mM(1):Wnt2 UTSW 6 18,008,622 (GRCm39) missense probably damaging 1.00
R1165:Wnt2 UTSW 6 17,989,946 (GRCm39) missense probably benign
R1771:Wnt2 UTSW 6 18,008,696 (GRCm39) missense probably damaging 0.97
R1782:Wnt2 UTSW 6 18,008,639 (GRCm39) missense possibly damaging 0.65
R1836:Wnt2 UTSW 6 18,023,234 (GRCm39) missense probably damaging 1.00
R1921:Wnt2 UTSW 6 18,030,252 (GRCm39) missense unknown
R2009:Wnt2 UTSW 6 18,030,208 (GRCm39) missense probably damaging 0.98
R4831:Wnt2 UTSW 6 18,023,285 (GRCm39) missense probably benign 0.19
R4888:Wnt2 UTSW 6 18,023,125 (GRCm39) missense possibly damaging 0.89
R4924:Wnt2 UTSW 6 18,023,239 (GRCm39) missense probably damaging 1.00
R5121:Wnt2 UTSW 6 18,023,125 (GRCm39) missense possibly damaging 0.89
R5660:Wnt2 UTSW 6 18,028,145 (GRCm39) missense probably benign 0.09
R5767:Wnt2 UTSW 6 17,990,027 (GRCm39) missense probably damaging 0.97
R6005:Wnt2 UTSW 6 18,030,322 (GRCm39) start gained probably benign
R6670:Wnt2 UTSW 6 18,028,091 (GRCm39) missense possibly damaging 0.90
R7205:Wnt2 UTSW 6 18,028,046 (GRCm39) missense probably benign 0.11
R7711:Wnt2 UTSW 6 17,990,036 (GRCm39) missense probably benign 0.44
R7732:Wnt2 UTSW 6 18,023,335 (GRCm39) missense probably damaging 1.00
R8249:Wnt2 UTSW 6 18,030,284 (GRCm39) start codon destroyed probably null
R9145:Wnt2 UTSW 6 18,030,397 (GRCm39) start gained probably benign
Predicted Primers PCR Primer
(F):5'- AGAAGCTCTGTCTCAGTGAGTC -3'
(R):5'- AGAACACTTGGAGAGACGCTC -3'

Sequencing Primer
(F):5'- CTCTGTCTCAGTGAGTCACAAGAG -3'
(R):5'- GCTCTCTGACCGCTTTTAAGAAAGG -3'
Posted On 2015-04-17