Mutagenetix > Incidental Mutation

Incidental Mutation 'R4002:Ube2frt'

311317

Institutional Source

Beutler Lab

Gene Symbol

Ube2frt

Ensembl Gene

ENSMUSG00000059301

Gene Name

ubiquitin-conjugating enzyme E2F (putative), retrotransposed

Synonyms

Gm5434

MMRRC Submission

041609-MU

Accession Numbers

Essential gene?

Not available question?

Stock #

R4002 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

36140378-36141828 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to A at 36140635 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000020856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020856]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000020856

SMART Domains

Protein: ENSMUSP00000020856
Gene: ENSMUSG00000020547

Domain	Start	End	E-Value	Type
eIF5C	326	411	3.29e-33	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000071825

SMART Domains

Protein: ENSMUSP00000071728
Gene: ENSMUSG00000059301

Domain	Start	End	E-Value	Type
UBCc	35	185	6.96e-36	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000222384

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.4%

Validation Efficiency

97% (29/30)

Allele List at MGI

Other mutations in this stock

Total: 27 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	T	A	7: 41,274,964 (GRCm39)	N222K	possibly damaging	Het
Abca13	A	T	11: 9,535,415 (GRCm39)	M4680L	probably benign	Het
Adgrv1	GA	GAA	13: 81,688,251 (GRCm39)		probably null	Het
Afdn	T	C	17: 14,104,179 (GRCm39)	S1157P	probably damaging	Het
Ank1	A	G	8: 23,629,479 (GRCm39)	T63A	probably damaging	Het
Asah1	A	G	8: 41,801,176 (GRCm39)		probably benign	Het
Cdh18	T	A	15: 23,383,048 (GRCm39)	L277I	possibly damaging	Het
Ces3a	A	T	8: 105,784,093 (GRCm39)	D431V	probably damaging	Het
Dbx1	T	C	7: 49,286,265 (GRCm39)	S67G	probably benign	Het
Dmxl2	A	G	9: 54,381,116 (GRCm39)		probably benign	Het
Dnah7a	T	A	1: 53,670,840 (GRCm39)	T471S	probably benign	Het
Efcab8	A	T	2: 153,623,726 (GRCm39)	K70N	probably benign	Het
Grcc10	A	T	6: 124,717,933 (GRCm39)	M1K	probably null	Het
Higd2a	G	C	13: 54,738,540 (GRCm39)	C53S	probably damaging	Het
Kcna4	C	T	2: 107,126,259 (GRCm39)	P331L	probably damaging	Het
Keg1	T	A	19: 12,696,307 (GRCm39)	S164T	possibly damaging	Het
Ltbp1	T	C	17: 75,617,154 (GRCm39)	V1031A	probably benign	Het
Obsl1	G	A	1: 75,476,743 (GRCm39)	T737I	possibly damaging	Het
Or10al7	G	A	17: 38,365,879 (GRCm39)	L193F	probably damaging	Het
Or7g33	C	T	9: 19,449,202 (GRCm39)	R8K	probably benign	Het
Or7g35	T	G	9: 19,496,010 (GRCm39)	M59R	probably damaging	Het
Serpinb6d	A	G	13: 33,854,630 (GRCm39)	M202V	probably damaging	Het
Tcf19	A	G	17: 35,826,822 (GRCm39)		probably null	Het
Tlr11	T	C	14: 50,599,984 (GRCm39)	F657L	probably benign	Het
Ttf2	G	A	3: 100,855,541 (GRCm39)	Q96*	probably null	Het
Zbbx	C	T	3: 75,012,978 (GRCm39)	G151E	probably damaging	Het
Zfp810	C	T	9: 22,190,188 (GRCm39)	C240Y	probably damaging	Het

Other mutations in Ube2frt

Allele	Source	Chr	Coord	Type	Predicted Effect
R0948:Ube2frt	UTSW	12	36,140,934 (GRCm39)	unclassified	probably benign
R1954:Ube2frt	UTSW	12	36,140,595 (GRCm39)	unclassified	probably benign
R1955:Ube2frt	UTSW	12	36,140,595 (GRCm39)	unclassified	probably benign
R2885:Ube2frt	UTSW	12	36,140,574 (GRCm39)	unclassified	probably benign
R2886:Ube2frt	UTSW	12	36,140,574 (GRCm39)	unclassified	probably benign
R3825:Ube2frt	UTSW	12	36,141,036 (GRCm39)	unclassified	probably benign
R4803:Ube2frt	UTSW	12	36,140,729 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- GATTCGTGGTGCTCAGAAAGG -3'
(R):5'- CAGCGGAATGTTCTCTTAGTAAAC -3'

Sequencing Primer
(F):5'- GTGGCAGTAATGCTAACGCTG -3'
(R):5'- GTAAACTTAGACATATTTCCCCCG -3'

Posted On

2015-04-29