Incidental Mutation 'R4011:Trim65'
ID 311703
Institutional Source Beutler Lab
Gene Symbol Trim65
Ensembl Gene ENSMUSG00000054517
Gene Name tripartite motif-containing 65
Synonyms 4732463G12Rik
MMRRC Submission 040948-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R4011 (G1)
Quality Score 225
Status Validated
Chromosome 11
Chromosomal Location 116012672-116021954 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 116018529 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Leucine at position 249 (F249L)
Ref Sequence ENSEMBL: ENSMUSP00000102048 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000067632] [ENSMUST00000106439] [ENSMUST00000106440]
AlphaFold Q8BFW4
Predicted Effect probably benign
Transcript: ENSMUST00000067632
AA Change: F249L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000063410
Gene: ENSMUSG00000054517
AA Change: F249L

DomainStartEndE-ValueType
RING 13 51 2.47e-9 SMART
low complexity region 69 85 N/A INTRINSIC
Blast:BBOX 94 132 3e-10 BLAST
coiled coil region 140 175 N/A INTRINSIC
low complexity region 282 298 N/A INTRINSIC
PRY 333 383 3.67e-3 SMART
Pfam:SPRY 386 505 1.5e-10 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000106439
SMART Domains Protein: ENSMUSP00000102047
Gene: ENSMUSG00000020775

DomainStartEndE-ValueType
low complexity region 55 67 N/A INTRINSIC
Pfam:PBP 183 313 3e-13 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000106440
AA Change: F249L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000102048
Gene: ENSMUSG00000054517
AA Change: F249L

DomainStartEndE-ValueType
RING 13 51 2.47e-9 SMART
low complexity region 69 85 N/A INTRINSIC
Blast:BBOX 94 132 2e-10 BLAST
coiled coil region 140 175 N/A INTRINSIC
low complexity region 282 298 N/A INTRINSIC
PRY 333 383 3.67e-3 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000133039
Predicted Effect noncoding transcript
Transcript: ENSMUST00000142550
Predicted Effect noncoding transcript
Transcript: ENSMUST00000154061
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 93.7%
Validation Efficiency 100% (43/43)
Allele List at MGI
Other mutations in this stock
Total: 43 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 G A 11: 9,572,013 (GRCm39) probably benign Het
Acss2 T A 2: 155,399,548 (GRCm39) L529Q probably damaging Het
Adsl T C 15: 80,850,357 (GRCm39) S359P probably benign Het
C1qtnf1 G A 11: 118,337,365 (GRCm39) G65D probably benign Het
Chd1l A T 3: 97,477,718 (GRCm39) M679K probably benign Het
Cspg4 A G 9: 56,794,601 (GRCm39) T779A probably benign Het
Ddx18 T C 1: 121,489,810 (GRCm39) I200V probably benign Het
Dnah9 T A 11: 65,725,290 (GRCm39) M4288L probably damaging Het
F830045P16Rik A T 2: 129,305,467 (GRCm39) N302K probably damaging Het
Gas1 T G 13: 60,323,791 (GRCm39) D322A unknown Het
Gfm2 T A 13: 97,279,608 (GRCm39) probably benign Het
Gm5592 G T 7: 40,938,934 (GRCm39) V739L probably benign Het
Hbb-bh2 T A 7: 103,489,416 (GRCm39) H45L probably benign Het
Ifit2 G T 19: 34,551,445 (GRCm39) M328I probably benign Het
Igdcc4 T C 9: 65,042,761 (GRCm39) V1237A probably benign Het
Kat2b G A 17: 53,951,769 (GRCm39) probably null Het
Lmtk2 A G 5: 144,112,697 (GRCm39) N1139S probably benign Het
Map1a G A 2: 121,130,608 (GRCm39) A475T probably damaging Het
Map3k20 G A 2: 72,214,468 (GRCm39) probably benign Het
Mga T C 2: 119,762,261 (GRCm39) V1084A probably damaging Het
Msantd5f6 T C 4: 73,320,047 (GRCm39) Y244C probably damaging Het
Myo3b A G 2: 69,926,720 (GRCm39) I5V probably benign Het
Or10a3m A T 7: 108,313,366 (GRCm39) I269L probably benign Het
Or10ag56 A G 2: 87,139,555 (GRCm39) I141V probably benign Het
Or10ag57 A T 2: 87,218,924 (GRCm39) I292F possibly damaging Het
Or56b1 A G 7: 104,285,555 (GRCm39) T225A probably benign Het
Or5h24 G A 16: 58,919,124 (GRCm39) T77I unknown Het
Or5v1 T C 17: 37,810,382 (GRCm39) V280A possibly damaging Het
Osmr G T 15: 6,854,014 (GRCm39) R565S probably benign Het
Pcolce G T 5: 137,604,036 (GRCm39) Q344K probably benign Het
Prdm11 A G 2: 92,843,175 (GRCm39) F95L probably damaging Het
Psd2 C T 18: 36,120,300 (GRCm39) T383I probably benign Het
Reg3a G A 6: 78,360,553 (GRCm39) D164N probably damaging Het
Serpina3a A T 12: 104,084,902 (GRCm39) D99V probably benign Het
Sgce G T 6: 4,691,563 (GRCm39) Y301* probably null Het
Slc28a3 T C 13: 58,714,064 (GRCm39) K434E probably benign Het
Slc30a3 A G 5: 31,244,203 (GRCm39) F360L probably damaging Het
Tdpoz3 A T 3: 93,733,550 (GRCm39) Y75F possibly damaging Het
Tubgcp3 A T 8: 12,689,634 (GRCm39) L544* probably null Het
Vit C T 17: 78,842,121 (GRCm39) probably benign Het
Wdfy4 C A 14: 32,824,637 (GRCm39) probably benign Het
Xpo6 T C 7: 125,739,780 (GRCm39) K431E probably benign Het
Xrn1 T A 9: 95,867,278 (GRCm39) Y545* probably null Het
Other mutations in Trim65
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01420:Trim65 APN 11 116,017,335 (GRCm39) missense probably damaging 1.00
PIT4531001:Trim65 UTSW 11 116,018,535 (GRCm39) missense possibly damaging 0.85
R0105:Trim65 UTSW 11 116,016,892 (GRCm39) makesense probably null
R0126:Trim65 UTSW 11 116,015,430 (GRCm39) splice site probably benign
R0268:Trim65 UTSW 11 116,017,470 (GRCm39) splice site probably benign
R0647:Trim65 UTSW 11 116,019,036 (GRCm39) missense possibly damaging 0.92
R2234:Trim65 UTSW 11 116,021,503 (GRCm39) missense possibly damaging 0.91
R2235:Trim65 UTSW 11 116,021,503 (GRCm39) missense possibly damaging 0.91
R4086:Trim65 UTSW 11 116,017,305 (GRCm39) nonsense probably null
R4088:Trim65 UTSW 11 116,017,305 (GRCm39) nonsense probably null
R4089:Trim65 UTSW 11 116,017,305 (GRCm39) nonsense probably null
R4434:Trim65 UTSW 11 116,018,435 (GRCm39) nonsense probably null
R5407:Trim65 UTSW 11 116,016,906 (GRCm39) missense probably benign
R5947:Trim65 UTSW 11 116,019,108 (GRCm39) missense probably damaging 0.99
R6299:Trim65 UTSW 11 116,017,377 (GRCm39) missense probably benign 0.00
R7248:Trim65 UTSW 11 116,018,534 (GRCm39) missense probably benign 0.01
R7336:Trim65 UTSW 11 116,019,116 (GRCm39) missense probably benign 0.00
R7496:Trim65 UTSW 11 116,017,142 (GRCm39) missense probably damaging 1.00
R7835:Trim65 UTSW 11 116,021,755 (GRCm39) missense probably damaging 1.00
R7849:Trim65 UTSW 11 116,017,082 (GRCm39) missense probably damaging 0.99
R8143:Trim65 UTSW 11 116,017,287 (GRCm39) missense probably benign 0.09
R8195:Trim65 UTSW 11 116,017,037 (GRCm39) missense probably benign 0.04
R8783:Trim65 UTSW 11 116,017,143 (GRCm39) missense probably damaging 1.00
R9158:Trim65 UTSW 11 116,018,050 (GRCm39) missense probably benign 0.01
R9740:Trim65 UTSW 11 116,021,434 (GRCm39) missense probably benign 0.26
R9751:Trim65 UTSW 11 116,021,564 (GRCm39) missense probably benign 0.01
X0061:Trim65 UTSW 11 116,017,397 (GRCm39) missense probably benign 0.39
X0066:Trim65 UTSW 11 116,021,672 (GRCm39) missense probably benign 0.25
Predicted Primers PCR Primer
(F):5'- TCAAGGAGGAGCTCACACAG -3'
(R):5'- GAGATTCAGTAGCCTGCTTCAG -3'

Sequencing Primer
(F):5'- CTAAGCAGCTGGTTCACGTTGC -3'
(R):5'- TGCTTCAGGCGCTGGAG -3'
Posted On 2015-04-29