Incidental Mutation 'R4021:Fstl5'
ID 313219
Institutional Source Beutler Lab
Gene Symbol Fstl5
Ensembl Gene ENSMUSG00000034098
Gene Name follistatin-like 5
Synonyms 9130207J01Rik
MMRRC Submission 040955-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.088) question?
Stock # R4021 (G1)
Quality Score 225
Status Validated
Chromosome 3
Chromosomal Location 75981582-76617317 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 76536282 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 31 (T31A)
Ref Sequence ENSEMBL: ENSMUSP00000125688 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000038364] [ENSMUST00000160261] [ENSMUST00000162471]
AlphaFold Q8BFR2
Predicted Effect probably benign
Transcript: ENSMUST00000038364
AA Change: T468A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000038506
Gene: ENSMUSG00000034098
AA Change: T468A

DomainStartEndE-ValueType
signal peptide 1 20 N/A INTRINSIC
KAZAL 88 133 2.16e-9 SMART
IGc2 261 328 1.11e-5 SMART
IGc2 353 420 3.85e-14 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000160261
AA Change: T468A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000125393
Gene: ENSMUSG00000034098
AA Change: T468A

DomainStartEndE-ValueType
signal peptide 1 20 N/A INTRINSIC
KAZAL 88 133 2.16e-9 SMART
IGc2 261 328 1.11e-5 SMART
IGc2 353 420 3.85e-14 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000162471
AA Change: T31A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.8%
  • 20x: 93.2%
Validation Efficiency 100% (65/65)
Allele List at MGI
Other mutations in this stock
Total: 59 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700008P02Rik A G 3: 6,685,148 (GRCm39) L102P probably benign Het
Adcy4 C T 14: 56,012,635 (GRCm39) probably null Het
Adgrf5 A T 17: 43,741,605 (GRCm39) probably benign Het
Atp11a A G 8: 12,892,938 (GRCm39) K643R probably benign Het
Cacna2d2 C T 9: 107,391,257 (GRCm39) T428M probably damaging Het
Cdh22 T C 2: 164,985,593 (GRCm39) D331G possibly damaging Het
Chmp3 T C 6: 71,551,222 (GRCm39) probably null Het
Csnk2a1 C T 2: 152,100,609 (GRCm39) T127M probably damaging Het
Cyp2c55 T A 19: 39,023,878 (GRCm39) probably null Het
Ddias G T 7: 92,510,686 (GRCm39) D105E possibly damaging Het
Dnajb11 A G 16: 22,688,196 (GRCm39) D238G probably damaging Het
Dock7 T C 4: 98,892,157 (GRCm39) probably null Het
Dock9 T C 14: 121,864,324 (GRCm39) K761E possibly damaging Het
Entpd7 G A 19: 43,679,597 (GRCm39) R50Q probably benign Het
Fam107b G A 2: 3,779,511 (GRCm39) R238Q probably damaging Het
Fam186a G C 15: 99,839,680 (GRCm39) T2188S possibly damaging Het
Farsa A G 8: 85,595,499 (GRCm39) T465A probably damaging Het
Fibp T C 19: 5,510,762 (GRCm39) probably null Het
Flywch2 G A 17: 23,996,013 (GRCm39) T128I possibly damaging Het
Foxi2 A T 7: 135,012,259 (GRCm39) D49V probably damaging Het
Gabbr2 G A 4: 46,846,435 (GRCm39) T158I probably damaging Het
Gbp4 T A 5: 105,268,789 (GRCm39) R455W probably damaging Het
Got2 T G 8: 96,604,381 (GRCm39) D69A probably damaging Het
Gpr63 T C 4: 25,008,470 (GRCm39) F398S possibly damaging Het
Gtf2h4 T C 17: 35,981,556 (GRCm39) M186V probably benign Het
Haus2 A T 2: 120,446,411 (GRCm39) Q111L probably damaging Het
Hexd T C 11: 121,108,987 (GRCm39) probably null Het
Igf2r T A 17: 12,967,638 (GRCm39) N27I probably damaging Het
Itgax T A 7: 127,732,311 (GRCm39) probably null Het
Krit1 T A 5: 3,882,132 (GRCm39) I596K probably benign Het
Lair1 A G 7: 4,058,915 (GRCm39) probably null Het
Lilra6 G T 7: 3,914,417 (GRCm39) T276K probably benign Het
Mast4 G A 13: 102,875,829 (GRCm39) R1112* probably null Het
Mrgprb1 A T 7: 48,096,871 (GRCm39) I347N possibly damaging Het
Mroh2b T A 15: 4,954,582 (GRCm39) C682S possibly damaging Het
Mtif3 T A 5: 146,892,488 (GRCm39) R249S possibly damaging Het
Mycbp2 C T 14: 103,389,593 (GRCm39) E3406K probably damaging Het
Myo15b A G 11: 115,764,331 (GRCm39) H1315R probably benign Het
Nlrp2 A G 7: 5,328,011 (GRCm39) F681L probably benign Het
Or2ag19 T A 7: 106,444,226 (GRCm39) M136K probably damaging Het
Or5l14 T A 2: 87,793,066 (GRCm39) T57S possibly damaging Het
Pear1 T C 3: 87,663,529 (GRCm39) N390D possibly damaging Het
Ranbp17 G A 11: 33,429,189 (GRCm39) A352V probably benign Het
Rnf17 C T 14: 56,697,458 (GRCm39) H451Y probably damaging Het
Sag A T 1: 87,749,027 (GRCm39) probably null Het
Septin4 A G 11: 87,458,106 (GRCm39) E160G probably damaging Het
Slc22a2 T C 17: 12,803,376 (GRCm39) L70P probably damaging Het
Slc32a1 C T 2: 158,453,152 (GRCm39) probably benign Het
Spag17 A T 3: 99,956,546 (GRCm39) I881F probably benign Het
Taar7a G T 10: 23,869,284 (GRCm39) N32K probably benign Het
Tbck C A 3: 132,432,895 (GRCm39) T435K probably damaging Het
Tril T G 6: 53,796,004 (GRCm39) D406A probably damaging Het
Tshz2 G A 2: 169,727,782 (GRCm39) D324N probably damaging Het
Vps13d A G 4: 144,801,631 (GRCm39) V2349A possibly damaging Het
Wdr6 A G 9: 108,452,405 (GRCm39) W493R probably damaging Het
Wdr72 G A 9: 74,058,875 (GRCm39) V323I probably benign Het
Zfp488 T A 14: 33,693,110 (GRCm39) M18L probably benign Het
Zic4 A G 9: 91,261,089 (GRCm39) T108A probably benign Het
Znrf3 T C 11: 5,231,278 (GRCm39) D745G possibly damaging Het
Other mutations in Fstl5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01632:Fstl5 APN 3 76,615,135 (GRCm39) missense probably benign 0.30
IGL01658:Fstl5 APN 3 76,389,562 (GRCm39) missense possibly damaging 0.70
IGL01917:Fstl5 APN 3 76,615,153 (GRCm39) missense probably damaging 1.00
IGL02073:Fstl5 APN 3 76,566,959 (GRCm39) splice site probably benign
IGL02329:Fstl5 APN 3 76,496,302 (GRCm39) missense probably damaging 1.00
IGL02651:Fstl5 APN 3 76,500,841 (GRCm39) missense probably damaging 1.00
IGL02967:Fstl5 APN 3 76,229,498 (GRCm39) missense probably damaging 1.00
IGL03004:Fstl5 APN 3 76,555,738 (GRCm39) splice site probably benign
IGL03107:Fstl5 APN 3 76,443,618 (GRCm39) missense probably damaging 1.00
IGL03113:Fstl5 APN 3 76,337,099 (GRCm39) nonsense probably null
P0038:Fstl5 UTSW 3 76,052,369 (GRCm39) missense probably damaging 1.00
PIT4131001:Fstl5 UTSW 3 76,567,006 (GRCm39) missense probably damaging 0.99
R0015:Fstl5 UTSW 3 76,229,498 (GRCm39) missense probably damaging 1.00
R0015:Fstl5 UTSW 3 76,229,498 (GRCm39) missense probably damaging 1.00
R0032:Fstl5 UTSW 3 76,555,742 (GRCm39) splice site probably benign
R0032:Fstl5 UTSW 3 76,555,742 (GRCm39) splice site probably benign
R0078:Fstl5 UTSW 3 76,566,952 (GRCm39) splice site probably benign
R0137:Fstl5 UTSW 3 76,614,786 (GRCm39) missense probably damaging 1.00
R0183:Fstl5 UTSW 3 76,229,579 (GRCm39) missense possibly damaging 0.86
R0330:Fstl5 UTSW 3 76,615,060 (GRCm39) missense possibly damaging 0.80
R0427:Fstl5 UTSW 3 76,615,034 (GRCm39) nonsense probably null
R0687:Fstl5 UTSW 3 76,615,119 (GRCm39) missense possibly damaging 0.62
R1642:Fstl5 UTSW 3 76,317,929 (GRCm39) missense possibly damaging 0.80
R1765:Fstl5 UTSW 3 76,500,783 (GRCm39) missense possibly damaging 0.90
R1900:Fstl5 UTSW 3 76,615,467 (GRCm39) missense probably damaging 1.00
R1996:Fstl5 UTSW 3 76,615,141 (GRCm39) missense probably benign 0.19
R2157:Fstl5 UTSW 3 76,615,372 (GRCm39) missense possibly damaging 0.46
R2228:Fstl5 UTSW 3 76,389,659 (GRCm39) missense probably damaging 1.00
R2851:Fstl5 UTSW 3 76,337,045 (GRCm39) splice site probably benign
R4086:Fstl5 UTSW 3 76,555,593 (GRCm39) missense probably damaging 1.00
R4777:Fstl5 UTSW 3 76,500,807 (GRCm39) missense probably damaging 1.00
R4829:Fstl5 UTSW 3 76,229,489 (GRCm39) missense probably damaging 1.00
R4934:Fstl5 UTSW 3 76,496,272 (GRCm39) missense probably damaging 1.00
R4955:Fstl5 UTSW 3 76,131,183 (GRCm39) critical splice donor site probably null
R4977:Fstl5 UTSW 3 76,317,801 (GRCm39) nonsense probably null
R5166:Fstl5 UTSW 3 76,536,267 (GRCm39) missense possibly damaging 0.86
R5232:Fstl5 UTSW 3 76,052,284 (GRCm39) missense possibly damaging 0.89
R5313:Fstl5 UTSW 3 76,500,812 (GRCm39) missense possibly damaging 0.90
R5584:Fstl5 UTSW 3 76,229,574 (GRCm39) missense probably damaging 1.00
R5647:Fstl5 UTSW 3 76,496,399 (GRCm39) missense probably damaging 1.00
R5842:Fstl5 UTSW 3 76,229,590 (GRCm39) missense possibly damaging 0.94
R5978:Fstl5 UTSW 3 76,052,392 (GRCm39) missense probably damaging 1.00
R6007:Fstl5 UTSW 3 76,317,899 (GRCm39) missense probably damaging 1.00
R6064:Fstl5 UTSW 3 76,229,605 (GRCm39) missense probably benign 0.13
R6327:Fstl5 UTSW 3 76,615,108 (GRCm39) missense probably benign 0.31
R6386:Fstl5 UTSW 3 76,229,373 (GRCm39) missense probably benign 0.13
R6523:Fstl5 UTSW 3 76,443,641 (GRCm39) missense probably benign 0.00
R6852:Fstl5 UTSW 3 76,615,162 (GRCm39) missense probably damaging 1.00
R6861:Fstl5 UTSW 3 76,229,523 (GRCm39) missense probably damaging 1.00
R6866:Fstl5 UTSW 3 76,229,532 (GRCm39) missense probably damaging 0.99
R7100:Fstl5 UTSW 3 76,443,600 (GRCm39) missense probably benign 0.11
R7341:Fstl5 UTSW 3 76,389,704 (GRCm39) splice site probably null
R7495:Fstl5 UTSW 3 76,615,099 (GRCm39) missense possibly damaging 0.85
R7558:Fstl5 UTSW 3 76,337,092 (GRCm39) missense possibly damaging 0.95
R7731:Fstl5 UTSW 3 76,569,069 (GRCm39) missense probably damaging 1.00
R7787:Fstl5 UTSW 3 76,337,131 (GRCm39) missense probably damaging 1.00
R7852:Fstl5 UTSW 3 76,615,275 (GRCm39) missense probably benign 0.00
R7874:Fstl5 UTSW 3 76,569,093 (GRCm39) missense probably benign 0.10
R7881:Fstl5 UTSW 3 76,443,605 (GRCm39) missense probably damaging 1.00
R7986:Fstl5 UTSW 3 76,337,097 (GRCm39) missense probably damaging 0.98
R8039:Fstl5 UTSW 3 76,555,725 (GRCm39) missense possibly damaging 0.69
R8050:Fstl5 UTSW 3 76,614,810 (GRCm39) missense probably benign 0.00
R8844:Fstl5 UTSW 3 76,337,154 (GRCm39) missense possibly damaging 0.71
R8929:Fstl5 UTSW 3 76,615,138 (GRCm39) missense probably damaging 0.98
R9012:Fstl5 UTSW 3 76,567,027 (GRCm39) missense probably damaging 1.00
R9069:Fstl5 UTSW 3 76,615,416 (GRCm39) missense probably damaging 0.99
R9221:Fstl5 UTSW 3 76,569,114 (GRCm39) missense probably damaging 0.98
R9373:Fstl5 UTSW 3 76,555,669 (GRCm39) nonsense probably null
R9427:Fstl5 UTSW 3 76,229,583 (GRCm39) missense
R9490:Fstl5 UTSW 3 76,615,060 (GRCm39) missense possibly damaging 0.80
R9603:Fstl5 UTSW 3 76,496,260 (GRCm39) missense probably damaging 1.00
Z1176:Fstl5 UTSW 3 76,615,289 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GTGTCTCATGTAAGGGAGGTCC -3'
(R):5'- AAAGCTTCATGAACTCATAGACAGC -3'

Sequencing Primer
(F):5'- GAGGTCCCTCTCATTTGTTTTATTAG -3'
(R):5'- TGAACTCATAGACAGCATTATGAAC -3'
Posted On 2015-04-30