Incidental Mutation 'R4023:Tas2r105'
| ID |
313346 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Tas2r105
|
| Ensembl Gene |
ENSMUSG00000051153 |
| Gene Name |
taste receptor, type 2, member 105 |
| Synonyms |
T2r5, T2R05, mGR05, T2R9, mt2r5, Tas2r5 |
| MMRRC Submission |
040957-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.049)
|
| Stock # |
R4023 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
6 |
| Chromosomal Location |
131663524-131664426 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 131663789 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Alanine
at position 213
(V213A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000058006
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000053652]
[ENSMUST00000072404]
[ENSMUST00000080619]
|
| AlphaFold |
Q9JKT4 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000053652
AA Change: V213A
PolyPhen 2
Score 0.020 (Sensitivity: 0.95; Specificity: 0.80)
|
| SMART Domains |
Protein: ENSMUSP00000058006
Gene: ENSMUSG00000051153
AA Change: V213A
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:TAS2R
|
1 |
298 |
9.4e-109 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000072404
|
| SMART Domains |
Protein: ENSMUSP00000072237
Gene: ENSMUSG00000061977
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:TAS2R
|
1 |
298 |
8.3e-102 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000080619
|
| SMART Domains |
Protein: ENSMUSP00000079453
Gene: ENSMUSG00000063478
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:TAS2R
|
1 |
298 |
8.1e-104 |
PFAM |
|
| Meta Mutation Damage Score |
0.0747 |
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.5%
- 10x: 96.8%
- 20x: 93.2%
|
| Validation Efficiency |
|
| MGI Phenotype |
PHENOTYPE: Mice homozygous for a null mutation respond normally to a variety of bitter, sweet, umami, salty and sour stimuli but show a striking and selective impairement in their ability to taste cycloheximide (a bitter tastant). In addition, homozygotes are no longer behaviorally averse to cycloheximide. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 33 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1110038F14Rik |
CGGG |
CGGGGGG |
15: 76,833,863 (GRCm39) |
|
probably benign |
Het |
| Acad8 |
T |
C |
9: 26,890,481 (GRCm39) |
I276V |
probably benign |
Het |
| Adgrg7 |
A |
T |
16: 56,550,661 (GRCm39) |
Y684N |
probably damaging |
Het |
| Akap9 |
C |
T |
5: 4,042,077 (GRCm39) |
T1370I |
possibly damaging |
Het |
| Akna |
C |
G |
4: 63,292,627 (GRCm39) |
G1094A |
probably benign |
Het |
| Catsperb |
C |
A |
12: 101,568,942 (GRCm39) |
Y871* |
probably null |
Het |
| Clcn4 |
T |
G |
7: 7,293,427 (GRCm39) |
Y443S |
probably damaging |
Het |
| Clcn6 |
T |
A |
4: 148,098,740 (GRCm39) |
T463S |
possibly damaging |
Het |
| Colec10 |
A |
G |
15: 54,325,947 (GRCm39) |
D259G |
probably damaging |
Het |
| Ctnna2 |
A |
T |
6: 77,613,827 (GRCm39) |
D254E |
possibly damaging |
Het |
| Depdc1a |
G |
A |
3: 159,221,786 (GRCm39) |
|
probably null |
Het |
| Dlec1 |
T |
C |
9: 118,966,408 (GRCm39) |
Y1126H |
probably damaging |
Het |
| Fezf2 |
A |
T |
14: 12,343,986 (GRCm38) |
C302S |
probably damaging |
Het |
| Flnc |
T |
C |
6: 29,451,634 (GRCm39) |
I1591T |
possibly damaging |
Het |
| Kidins220 |
A |
G |
12: 25,107,143 (GRCm39) |
|
probably null |
Het |
| Mctp2 |
C |
A |
7: 71,739,987 (GRCm39) |
C801F |
possibly damaging |
Het |
| Myo1e |
A |
G |
9: 70,232,157 (GRCm39) |
I229V |
probably benign |
Het |
| Or5t17 |
A |
T |
2: 86,833,266 (GRCm39) |
K318* |
probably null |
Het |
| Or8c17 |
G |
A |
9: 38,180,757 (GRCm39) |
C308Y |
possibly damaging |
Het |
| Ppp3r1 |
T |
C |
11: 17,144,786 (GRCm39) |
V133A |
probably damaging |
Het |
| Rif1 |
T |
A |
2: 52,011,099 (GRCm39) |
Y2389N |
probably benign |
Het |
| Sidt1 |
A |
G |
16: 44,102,249 (GRCm39) |
S304P |
possibly damaging |
Het |
| Siglecf |
T |
A |
7: 43,004,995 (GRCm39) |
S408R |
possibly damaging |
Het |
| Stat5a |
T |
A |
11: 100,765,752 (GRCm39) |
C279* |
probably null |
Het |
| Tasor2 |
T |
C |
13: 3,634,554 (GRCm39) |
D751G |
probably damaging |
Het |
| Tektl1 |
T |
C |
10: 78,588,727 (GRCm39) |
T28A |
probably benign |
Het |
| Top2b |
T |
G |
14: 16,409,189 (GRCm38) |
I777M |
probably damaging |
Het |
| Ugcg |
C |
T |
4: 59,207,798 (GRCm39) |
P46S |
probably benign |
Het |
| Usp46 |
A |
G |
5: 74,193,136 (GRCm39) |
Y47H |
probably damaging |
Het |
| Vmn1r34 |
T |
A |
6: 66,614,688 (GRCm39) |
M17L |
probably benign |
Het |
| Vmn2r120 |
T |
A |
17: 57,843,718 (GRCm39) |
D42V |
possibly damaging |
Het |
| Wdr20 |
A |
G |
12: 110,759,950 (GRCm39) |
T279A |
probably benign |
Het |
| Zfyve1 |
A |
G |
12: 83,641,296 (GRCm39) |
V120A |
probably benign |
Het |
|
| Other mutations in Tas2r105 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01107:Tas2r105
|
APN |
6 |
131,664,074 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL01148:Tas2r105
|
APN |
6 |
131,663,815 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02882:Tas2r105
|
APN |
6 |
131,664,143 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R0833:Tas2r105
|
UTSW |
6 |
131,664,393 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0836:Tas2r105
|
UTSW |
6 |
131,664,393 (GRCm39) |
missense |
probably benign |
0.01 |
|
R1429:Tas2r105
|
UTSW |
6 |
131,663,904 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2010:Tas2r105
|
UTSW |
6 |
131,664,365 (GRCm39) |
missense |
probably benign |
0.41 |
|
R2418:Tas2r105
|
UTSW |
6 |
131,664,410 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4026:Tas2r105
|
UTSW |
6 |
131,663,789 (GRCm39) |
missense |
probably benign |
0.02 |
|
R4742:Tas2r105
|
UTSW |
6 |
131,663,814 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5497:Tas2r105
|
UTSW |
6 |
131,663,805 (GRCm39) |
splice site |
probably null |
|
|
R5812:Tas2r105
|
UTSW |
6 |
131,663,836 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R7191:Tas2r105
|
UTSW |
6 |
131,663,945 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7236:Tas2r105
|
UTSW |
6 |
131,663,723 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7482:Tas2r105
|
UTSW |
6 |
131,663,972 (GRCm39) |
missense |
probably benign |
0.10 |
|
R8783:Tas2r105
|
UTSW |
6 |
131,663,732 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R8986:Tas2r105
|
UTSW |
6 |
131,663,913 (GRCm39) |
nonsense |
probably null |
|
|
R9250:Tas2r105
|
UTSW |
6 |
131,663,951 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9580:Tas2r105
|
UTSW |
6 |
131,663,699 (GRCm39) |
missense |
probably damaging |
0.97 |
|
X0067:Tas2r105
|
UTSW |
6 |
131,664,233 (GRCm39) |
missense |
probably benign |
0.29 |
|
| Predicted Primers |
PCR Primer
(F):5'- CAAAGGCTTGCTTTAGCTGG -3'
(R):5'- AGTGAATCATAGAAACAGGACCTC -3'
Sequencing Primer
(F):5'- CAGCAAGGATATATGGATGCAGTTG -3'
(R):5'- ACCTCGGAGATGTACTGGG -3'
|
| Posted On |
2015-04-30 |
Incidental Mutation