Mutagenetix > Incidental Mutation

Incidental Mutation 'R4024:Vmn2r6'

313381

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r6

Ensembl Gene

ENSMUSG00000090581

Gene Name

vomeronasal 2, receptor 6

Synonyms

EG667069, EG620718

MMRRC Submission

041612-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.198) question?

Stock #

R4024 (G1)

Quality Score

188

Status

Validated

Chromosome

Chromosomal Location

64444916-64472855 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 64445671 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 685 (S685T)

Ref Sequence

ENSEMBL: ENSMUSP00000135148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165012] [ENSMUST00000176481]

AlphaFold

H3BK29

Predicted Effect

probably benign
Transcript: ENSMUST00000165012
AA Change: S596T

PolyPhen 2 Score 0.129 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000131831
Gene: ENSMUSG00000090581
AA Change: S596T

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	1.4e-72	PFAM
Pfam:Peripla_BP_6	58	244	1.2e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	3.9e-76	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000176481
AA Change: S685T

PolyPhen 2 Score 0.732 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000135148
Gene: ENSMUSG00000090581
AA Change: S685T

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	9.8e-77	PFAM
Pfam:Peripla_BP_6	142	331	3.4e-10	PFAM
Pfam:NCD3G	547	600	5.4e-17	PFAM
Pfam:7tm_3	633	867	3.9e-47	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.5%

Validation Efficiency

98% (58/59)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn1	A	G	12: 80,215,251 (GRCm39)	Y842H	probably damaging	Het
Adamts9	A	G	6: 92,849,765 (GRCm39)		probably benign	Het
Adgrg7	A	T	16: 56,550,661 (GRCm39)	Y684N	probably damaging	Het
Aoc1	A	T	6: 48,885,203 (GRCm39)	N646I	probably damaging	Het
Armc2	A	G	10: 41,869,054 (GRCm39)	S37P	probably benign	Het
Bhmt2	G	A	13: 93,799,839 (GRCm39)		probably benign	Het
Bpifb1	A	T	2: 154,054,966 (GRCm39)	D286V	probably damaging	Het
Cadps	T	A	14: 12,705,539 (GRCm38)	E285D	probably damaging	Het
Cap2	C	T	13: 46,791,317 (GRCm39)		probably benign	Het
Clcn4	T	G	7: 7,293,427 (GRCm39)	Y443S	probably damaging	Het
Clcn6	T	A	4: 148,098,740 (GRCm39)	T463S	possibly damaging	Het
Cmip	A	G	8: 118,174,155 (GRCm39)	I412V	possibly damaging	Het
Cndp1	T	C	18: 84,646,938 (GRCm39)	D250G	probably damaging	Het
Colec10	A	G	15: 54,325,947 (GRCm39)	D259G	probably damaging	Het
Ctnna2	A	T	6: 77,613,827 (GRCm39)	D254E	possibly damaging	Het
Dlec1	T	C	9: 118,966,408 (GRCm39)	Y1126H	probably damaging	Het
Dzank1	T	C	2: 144,324,147 (GRCm39)	S565G	probably benign	Het
Eef2k	A	G	7: 120,457,821 (GRCm39)	Y60C	probably benign	Het
Fbxl4	T	C	4: 22,377,074 (GRCm39)	V170A	possibly damaging	Het
Foxk2	T	A	11: 121,176,439 (GRCm39)	I195N	possibly damaging	Het
Gm10608	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	9: 118,989,784 (GRCm39)		probably benign	Het
Gpr6	G	A	10: 40,947,264 (GRCm39)	T106M	probably damaging	Het
Grk3	T	A	5: 113,062,850 (GRCm39)	N666Y	possibly damaging	Het
Hnmt	T	C	2: 23,893,777 (GRCm39)	D239G	probably benign	Het
Igf2	A	G	7: 142,208,044 (GRCm39)	V111A	probably benign	Het
Lrriq3	G	A	3: 154,893,939 (GRCm39)	E547K	probably benign	Het
Lrriq4	T	C	3: 30,704,422 (GRCm39)	V150A	possibly damaging	Het
Mroh8	A	G	2: 157,098,272 (GRCm39)	V292A	probably benign	Het
Myo1e	A	G	9: 70,232,157 (GRCm39)	I229V	probably benign	Het
Nisch	A	G	14: 30,898,776 (GRCm39)		probably benign	Het
Nkx2-2	T	C	2: 147,026,154 (GRCm39)	T195A	probably benign	Het
Or10aa3	C	T	1: 173,878,683 (GRCm39)	T248I	probably benign	Het
Or2t47	G	A	11: 58,442,222 (GRCm39)	T281I	possibly damaging	Het
Or5p63	A	T	7: 107,810,949 (GRCm39)	Y262*	probably null	Het
Or8w1	T	A	2: 87,465,499 (GRCm39)	L197F	probably damaging	Het
Plekhn1	A	G	4: 156,309,207 (GRCm39)	V233A	probably damaging	Het
Ppp3r1	T	C	11: 17,144,786 (GRCm39)	V133A	probably damaging	Het
Sash1	T	C	10: 8,605,681 (GRCm39)	D903G	probably benign	Het
Scn8a	A	G	15: 100,937,674 (GRCm39)	D1681G	probably damaging	Het
Slk	A	G	19: 47,610,809 (GRCm39)		probably null	Het
Tasor2	T	C	13: 3,634,554 (GRCm39)	D751G	probably damaging	Het
Tlr11	C	T	14: 50,600,303 (GRCm39)	T763I	probably benign	Het
Ttbk2	T	C	2: 120,590,736 (GRCm39)	T308A	possibly damaging	Het
Tyk2	G	A	9: 21,027,215 (GRCm39)	L552F	probably damaging	Het
Ubp1	A	G	9: 113,773,951 (GRCm39)	D50G	probably benign	Het
Ugcg	C	T	4: 59,207,798 (GRCm39)	P46S	probably benign	Het
Ulk4	A	T	9: 120,873,915 (GRCm39)	I1172N	possibly damaging	Het
Usf3	T	A	16: 44,036,528 (GRCm39)	V336E	possibly damaging	Het
Vangl2	T	C	1: 171,835,608 (GRCm39)	S355G	probably benign	Het
Vmn1r216	A	G	13: 23,284,061 (GRCm39)	D248G	probably damaging	Het
Vmn1r34	T	A	6: 66,614,688 (GRCm39)	M17L	probably benign	Het
Vmn2r120	T	A	17: 57,843,718 (GRCm39)	D42V	possibly damaging	Het
Vsig10l	T	C	7: 43,117,510 (GRCm39)	V701A	probably benign	Het
Wdfy3	A	G	5: 102,071,961 (GRCm39)		probably benign	Het
Zfp808	T	A	13: 62,319,544 (GRCm39)	C258S	possibly damaging	Het
Zfp988	A	C	4: 147,417,242 (GRCm39)	K559Q	probably benign	Het

Other mutations in Vmn2r6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01547:Vmn2r6	APN	3	64,445,525 (GRCm39)	missense	probably damaging	1.00
IGL01968:Vmn2r6	APN	3	64,463,766 (GRCm39)	missense	possibly damaging	0.94
IGL02009:Vmn2r6	APN	3	64,445,323 (GRCm39)	missense	possibly damaging	0.61
IGL02039:Vmn2r6	APN	3	64,463,610 (GRCm39)	missense	probably damaging	1.00
IGL02652:Vmn2r6	APN	3	64,463,749 (GRCm39)	missense	probably benign	0.24
IGL02737:Vmn2r6	APN	3	64,463,911 (GRCm39)	missense	possibly damaging	0.55
IGL02808:Vmn2r6	APN	3	64,463,917 (GRCm39)	missense	probably damaging	1.00
IGL03066:Vmn2r6	APN	3	64,472,574 (GRCm39)	missense	probably damaging	0.99
IGL03331:Vmn2r6	APN	3	64,445,428 (GRCm39)	missense	probably damaging	1.00
BB010:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
BB020:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
R0010:Vmn2r6	UTSW	3	64,466,966 (GRCm39)	nonsense	probably null
R0206:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0206:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0208:Vmn2r6	UTSW	3	64,447,333 (GRCm39)	missense	probably benign
R0427:Vmn2r6	UTSW	3	64,467,008 (GRCm39)	missense	probably damaging	1.00
R0466:Vmn2r6	UTSW	3	64,463,723 (GRCm39)	missense	probably damaging	1.00
R1018:Vmn2r6	UTSW	3	64,464,261 (GRCm39)	missense	probably benign	0.00
R1104:Vmn2r6	UTSW	3	64,445,487 (GRCm39)	missense	possibly damaging	0.93
R1186:Vmn2r6	UTSW	3	64,472,488 (GRCm39)	missense	probably benign	0.01
R1245:Vmn2r6	UTSW	3	64,464,211 (GRCm39)	missense	possibly damaging	0.53
R1295:Vmn2r6	UTSW	3	64,445,694 (GRCm39)	missense	probably damaging	1.00
R1473:Vmn2r6	UTSW	3	64,445,579 (GRCm39)	nonsense	probably null
R1498:Vmn2r6	UTSW	3	64,463,890 (GRCm39)	missense	probably damaging	1.00
R1925:Vmn2r6	UTSW	3	64,463,698 (GRCm39)	missense	possibly damaging	0.87
R2044:Vmn2r6	UTSW	3	64,445,262 (GRCm39)	missense	probably damaging	0.96
R2069:Vmn2r6	UTSW	3	64,463,519 (GRCm39)	missense	possibly damaging	0.89
R2253:Vmn2r6	UTSW	3	64,467,139 (GRCm39)	missense	probably damaging	1.00
R2261:Vmn2r6	UTSW	3	64,464,090 (GRCm39)	missense	probably benign	0.24
R2262:Vmn2r6	UTSW	3	64,464,090 (GRCm39)	missense	probably benign	0.24
R2350:Vmn2r6	UTSW	3	64,463,773 (GRCm39)	missense	probably benign	0.01
R2680:Vmn2r6	UTSW	3	64,445,707 (GRCm39)	missense	possibly damaging	0.91
R2846:Vmn2r6	UTSW	3	64,464,211 (GRCm39)	missense	possibly damaging	0.53
R2860:Vmn2r6	UTSW	3	64,454,760 (GRCm39)	missense	probably benign	0.00
R2861:Vmn2r6	UTSW	3	64,454,760 (GRCm39)	missense	probably benign	0.00
R3766:Vmn2r6	UTSW	3	64,463,929 (GRCm39)	missense	probably benign	0.19
R3870:Vmn2r6	UTSW	3	64,464,042 (GRCm39)	missense	probably damaging	0.96
R4018:Vmn2r6	UTSW	3	64,463,893 (GRCm39)	missense	probably benign	0.05
R4026:Vmn2r6	UTSW	3	64,445,671 (GRCm39)	missense	possibly damaging	0.73
R4227:Vmn2r6	UTSW	3	64,445,369 (GRCm39)	missense	probably damaging	0.99
R4526:Vmn2r6	UTSW	3	64,445,145 (GRCm39)	missense	probably benign	0.32
R4570:Vmn2r6	UTSW	3	64,467,068 (GRCm39)	missense	probably benign	0.31
R4894:Vmn2r6	UTSW	3	64,454,829 (GRCm39)	missense	probably benign
R4934:Vmn2r6	UTSW	3	64,463,766 (GRCm39)	missense	probably damaging	0.99
R5057:Vmn2r6	UTSW	3	64,445,207 (GRCm39)	missense	probably damaging	1.00
R5059:Vmn2r6	UTSW	3	64,445,044 (GRCm39)	missense	possibly damaging	0.89
R5148:Vmn2r6	UTSW	3	64,464,015 (GRCm39)	missense	probably damaging	0.99
R5155:Vmn2r6	UTSW	3	64,445,935 (GRCm39)	missense	probably benign	0.44
R5179:Vmn2r6	UTSW	3	64,445,411 (GRCm39)	missense	probably benign	0.00
R5256:Vmn2r6	UTSW	3	64,464,263 (GRCm39)	missense	probably benign	0.33
R5861:Vmn2r6	UTSW	3	64,463,454 (GRCm39)	missense	probably benign	0.00
R5950:Vmn2r6	UTSW	3	64,472,652 (GRCm39)	missense	probably benign	0.05
R6081:Vmn2r6	UTSW	3	64,463,953 (GRCm39)	missense	probably benign	0.25
R6173:Vmn2r6	UTSW	3	64,467,176 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r6	UTSW	3	64,445,424 (GRCm39)	missense	probably benign	0.04
R6240:Vmn2r6	UTSW	3	64,464,226 (GRCm39)	missense	probably damaging	1.00
R6433:Vmn2r6	UTSW	3	64,454,801 (GRCm39)	nonsense	probably null
R6645:Vmn2r6	UTSW	3	64,464,297 (GRCm39)	missense	probably damaging	1.00
R6791:Vmn2r6	UTSW	3	64,445,580 (GRCm39)	missense	probably damaging	1.00
R7265:Vmn2r6	UTSW	3	64,464,195 (GRCm39)	missense	probably benign	0.00
R7503:Vmn2r6	UTSW	3	64,447,372 (GRCm39)	nonsense	probably null
R7562:Vmn2r6	UTSW	3	64,463,941 (GRCm39)	missense	probably benign	0.00
R7584:Vmn2r6	UTSW	3	64,472,683 (GRCm39)	missense	probably benign	0.07
R7611:Vmn2r6	UTSW	3	64,472,563 (GRCm39)	missense	probably damaging	0.98
R7759:Vmn2r6	UTSW	3	64,463,991 (GRCm39)	missense	probably damaging	1.00
R7834:Vmn2r6	UTSW	3	64,445,443 (GRCm39)	missense	probably damaging	1.00
R7933:Vmn2r6	UTSW	3	64,467,224 (GRCm39)	missense	probably benign	0.02
R7982:Vmn2r6	UTSW	3	64,467,241 (GRCm39)	missense	probably damaging	1.00
R8024:Vmn2r6	UTSW	3	64,467,245 (GRCm39)	missense	probably benign	0.40
R8074:Vmn2r6	UTSW	3	64,455,064 (GRCm39)	intron	probably benign
R8169:Vmn2r6	UTSW	3	64,447,310 (GRCm39)	missense	probably benign	0.01
R8337:Vmn2r6	UTSW	3	64,463,526 (GRCm39)	nonsense	probably null
R8736:Vmn2r6	UTSW	3	64,467,221 (GRCm39)	missense	probably damaging	1.00
R8962:Vmn2r6	UTSW	3	64,463,576 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn2r6	UTSW	3	64,464,277 (GRCm39)	missense	probably benign	0.12
R9206:Vmn2r6	UTSW	3	64,467,032 (GRCm39)	missense	probably damaging	0.97
R9295:Vmn2r6	UTSW	3	64,463,484 (GRCm39)	missense	probably benign	0.00
R9332:Vmn2r6	UTSW	3	64,454,671 (GRCm39)	missense	probably benign	0.01
R9616:Vmn2r6	UTSW	3	64,445,724 (GRCm39)	missense	probably damaging	1.00
R9663:Vmn2r6	UTSW	3	64,463,549 (GRCm39)	missense	possibly damaging	0.90
R9685:Vmn2r6	UTSW	3	64,464,081 (GRCm39)	missense	probably benign	0.19
X0020:Vmn2r6	UTSW	3	64,445,871 (GRCm39)	missense	probably benign
X0066:Vmn2r6	UTSW	3	64,454,799 (GRCm39)	missense	probably damaging	1.00
Z1176:Vmn2r6	UTSW	3	64,463,746 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GTTGTGCATACACCAATCTCCC -3'
(R):5'- TTGCTTATGAGGAGGCCCTG -3'

Sequencing Primer
(F):5'- TCTCCCCTACAACACAGATCAG -3'
(R):5'- AGGAGGCCCTGGGGTTC -3'

Posted On

2015-04-30