Mutagenetix > Incidental Mutation

Incidental Mutation 'R4037:Ogfrl1'

313736

Institutional Source

Beutler Lab

Gene Symbol

Ogfrl1

Ensembl Gene

ENSMUSG00000026158

Gene Name

opioid growth factor receptor-like 1

Synonyms

2210417C17Rik

MMRRC Submission

040964-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R4037 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23405505-23422282 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 23418045 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000139453 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027343] [ENSMUST00000188677]

AlphaFold

Q8VE52

Predicted Effect

probably benign
Transcript: ENSMUST00000027343

SMART Domains

Protein: ENSMUSP00000027343
Gene: ENSMUSG00000026158

Domain	Start	End	E-Value	Type
low complexity region	28	44	N/A	INTRINSIC
low complexity region	72	86	N/A	INTRINSIC
Pfam:OGFr_N	114	320	1.7e-104	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186064

Predicted Effect

probably benign
Transcript: ENSMUST00000188677

SMART Domains

Protein: ENSMUSP00000139453
Gene: ENSMUSG00000026158

Domain	Start	End	E-Value	Type
Pfam:OGFr_N	1	92	1.6e-38	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.5%

Validation Efficiency

93% (37/40)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy8	A	G	15: 64,597,319 (GRCm39)	F881L	probably benign	Het
Capn6	A	G	X: 142,590,859 (GRCm39)	W291R	probably damaging	Het
Cdc27	T	C	11: 104,398,033 (GRCm39)	I776V	probably damaging	Het
Cpne5	C	T	17: 29,378,087 (GRCm39)	R564H	unknown	Het
Crnkl1	T	A	2: 145,774,247 (GRCm39)	D72V	possibly damaging	Het
Dcc	A	G	18: 72,483,468 (GRCm39)	L17P	possibly damaging	Het
Dhcr24	T	C	4: 106,431,075 (GRCm39)	F255L	probably benign	Het
Eef2kmt	A	T	16: 5,063,135 (GRCm39)	V335D	probably damaging	Het
Efcc1	C	T	6: 87,708,490 (GRCm39)	Q206*	probably null	Het
Glb1l3	A	C	9: 26,740,343 (GRCm39)	M329R	probably damaging	Het
Gpr137c	C	A	14: 45,457,687 (GRCm39)	L80I	probably damaging	Het
Hcls1	A	G	16: 36,776,987 (GRCm39)	T226A	possibly damaging	Het
Hmcn1	T	A	1: 150,648,253 (GRCm39)	T678S	probably benign	Het
Hspa2	T	C	12: 76,452,542 (GRCm39)	V412A	probably damaging	Het
Hspb8	A	G	5: 116,547,403 (GRCm39)	V193A	probably benign	Het
Man1c1	C	T	4: 134,320,650 (GRCm39)	D217N	probably damaging	Het
Metrn	T	C	17: 26,013,984 (GRCm39)	T281A	probably benign	Het
Mmachc	T	A	4: 116,563,215 (GRCm39)	T47S	probably damaging	Het
Ncoa6	G	A	2: 155,249,290 (GRCm39)	S1338L	probably damaging	Het
Or4a73	C	T	2: 89,421,379 (GRCm39)	V27I	probably benign	Het
Or4c113	A	G	2: 88,885,032 (GRCm39)	V246A	probably damaging	Het
Pax4	A	G	6: 28,443,882 (GRCm39)	I241T	probably benign	Het
Prkaa2	T	C	4: 104,908,444 (GRCm39)	N144D	probably damaging	Het
Rims1	T	C	1: 22,514,793 (GRCm39)	S537G	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Ruvbl1	T	A	6: 88,450,117 (GRCm39)	M96K	probably damaging	Het
Sdk2	T	C	11: 113,685,881 (GRCm39)	I1880V	probably damaging	Het
Sis	T	C	3: 72,835,935 (GRCm39)	N885D	probably benign	Het
Skint5	G	T	4: 113,743,011 (GRCm39)	T352K	unknown	Het
Slc16a10	G	C	10: 39,932,620 (GRCm39)	H314D	possibly damaging	Het
Slc36a2	T	C	11: 55,055,101 (GRCm39)	D318G	probably benign	Het
Slc38a4	G	A	15: 96,894,923 (GRCm39)	A531V	probably benign	Het
Stat3	C	T	11: 100,783,951 (GRCm39)	G658R	probably damaging	Het
Tnfrsf11a	A	G	1: 105,755,464 (GRCm39)		probably null	Het
Tnk2	G	T	16: 32,489,614 (GRCm39)	A298S	probably damaging	Het
Vmn1r218	T	C	13: 23,320,971 (GRCm39)	V26A	possibly damaging	Het
Wipf3	G	A	6: 54,458,813 (GRCm39)	G56D	probably damaging	Het

Other mutations in Ogfrl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00913:Ogfrl1	APN	1	23,409,171 (GRCm39)	missense	probably benign
IGL02200:Ogfrl1	APN	1	23,409,150 (GRCm39)	missense	probably benign	0.00
PIT4508001:Ogfrl1	UTSW	1	23,409,351 (GRCm39)	nonsense	probably null
R0012:Ogfrl1	UTSW	1	23,409,206 (GRCm39)	missense	possibly damaging	0.83
R0735:Ogfrl1	UTSW	1	23,414,835 (GRCm39)	missense	possibly damaging	0.76
R1474:Ogfrl1	UTSW	1	23,414,890 (GRCm39)	missense	probably damaging	1.00
R3837:Ogfrl1	UTSW	1	23,409,041 (GRCm39)	missense	probably benign	0.03
R4039:Ogfrl1	UTSW	1	23,418,045 (GRCm39)	splice site	probably benign
R4332:Ogfrl1	UTSW	1	23,414,910 (GRCm39)	missense	probably damaging	1.00
R4780:Ogfrl1	UTSW	1	23,409,402 (GRCm39)	missense	probably damaging	1.00
R5056:Ogfrl1	UTSW	1	23,418,130 (GRCm39)	missense	probably damaging	0.97
R5994:Ogfrl1	UTSW	1	23,418,070 (GRCm39)	missense	probably damaging	1.00
R6167:Ogfrl1	UTSW	1	23,415,309 (GRCm39)	missense	probably damaging	1.00
R6340:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6341:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6342:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6343:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6363:Ogfrl1	UTSW	1	23,409,194 (GRCm39)	missense	probably benign	0.01
R6584:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6586:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R7419:Ogfrl1	UTSW	1	23,422,063 (GRCm39)	nonsense	probably null
R8364:Ogfrl1	UTSW	1	23,414,824 (GRCm39)	nonsense	probably null
R8749:Ogfrl1	UTSW	1	23,409,399 (GRCm39)	missense	probably damaging	1.00
R9539:Ogfrl1	UTSW	1	23,415,322 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTTGCTCAAGTCATTTTGATATCGG -3'
(R):5'- GGTTGGAAATGCTCAGGGATC -3'

Sequencing Primer
(F):5'- CGGATATCTTTGAAGTTCTGCTAGAG -3'
(R):5'- AAATGCTCAGGGATCTTTATGGAG -3'

Posted On

2015-04-30