Mutagenetix > Incidental Mutation

Incidental Mutation 'R4152:Rsad1'

314963

Institutional Source

Beutler Lab

Gene Symbol

Rsad1

Ensembl Gene

ENSMUSG00000039096

Gene Name

radical S-adenosyl methionine domain containing 1

Synonyms

MMRRC Submission

040996-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R4152 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

94430624-94440081 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

T to C at 94439449 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000037361 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040487]

AlphaFold

Q5SUV1

Predicted Effect

probably benign
Transcript: ENSMUST00000040487

SMART Domains

Protein: ENSMUSP00000037361
Gene: ENSMUSG00000039096

Domain	Start	End	E-Value	Type
low complexity region	6	24	N/A	INTRINSIC
Elp3	39	259	6.54e-40	SMART
Pfam:HemN_C	346	414	7.7e-13	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132274

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143299

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148888

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.7%

Validation Efficiency

96% (45/47)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acaca	T	G	11: 84,183,752 (GRCm39)	M31R	possibly damaging	Het
Akap6	A	C	12: 53,187,190 (GRCm39)	S1535R	probably benign	Het
Ap3b2	A	G	7: 81,127,765 (GRCm39)	I137T	probably damaging	Het
Cdc16	A	G	8: 13,812,857 (GRCm39)	S36G	probably damaging	Het
Clcn4	T	C	7: 7,297,833 (GRCm39)	N67D	probably benign	Het
Col4a1	T	C	8: 11,267,227 (GRCm39)		probably null	Het
Crem	G	T	18: 3,288,055 (GRCm39)	N179K	probably damaging	Het
Fam78b	T	C	1: 166,906,369 (GRCm39)	M176T	probably benign	Het
Fcgbpl1	T	A	7: 27,856,322 (GRCm39)	H2036Q	possibly damaging	Het
Gcn1	A	G	5: 115,751,413 (GRCm39)		probably null	Het
Gm6483	T	C	8: 19,737,926 (GRCm39)		noncoding transcript	Het
Klk14	G	A	7: 43,341,501 (GRCm39)	C51Y	probably damaging	Het
Klk1b16	T	C	7: 43,789,973 (GRCm39)	F81S	probably benign	Het
Lpgat1	C	A	1: 191,451,600 (GRCm39)	Y36*	probably null	Het
Mavs	A	G	2: 131,088,528 (GRCm39)	D444G	probably benign	Het
Ndst3	T	C	3: 123,465,876 (GRCm39)	Y32C	probably damaging	Het
Nemp2	A	G	1: 52,680,210 (GRCm39)	S145G	probably benign	Het
Or52b1	T	C	7: 104,978,592 (GRCm39)	N269S	probably damaging	Het
Or7g20	T	A	9: 18,946,816 (GRCm39)	Y132*	probably null	Het
Or8g52	A	C	9: 39,631,296 (GRCm39)	M258L	probably benign	Het
Pds5a	A	T	5: 65,823,514 (GRCm39)	C92*	probably null	Het
Pgk2	A	G	17: 40,519,149 (GRCm39)	V93A	probably damaging	Het
Phf3	C	T	1: 30,870,539 (GRCm39)	V116I	probably benign	Het
Prl8a2	T	C	13: 27,534,985 (GRCm39)	Y86H	possibly damaging	Het
Rab4b	T	C	7: 26,875,551 (GRCm39)		probably benign	Het
Sim1	G	A	10: 50,859,950 (GRCm39)	C604Y	probably damaging	Het
Slc5a3	T	A	16: 91,874,696 (GRCm39)	L251*	probably null	Het
Slit3	A	G	11: 35,589,147 (GRCm39)	N1234S	probably damaging	Het
Sntg1	A	T	1: 8,653,569 (GRCm39)		probably null	Het
Snx31	T	C	15: 36,525,785 (GRCm39)	N305D	probably benign	Het
St14	T	C	9: 31,001,802 (GRCm39)	I768V	probably benign	Het
Tep1	G	A	14: 51,075,051 (GRCm39)	H1755Y	possibly damaging	Het
Tlr6	A	C	5: 65,110,555 (GRCm39)	F784C	probably damaging	Het
Tmem132a	A	G	19: 10,836,427 (GRCm39)	V701A	probably benign	Het
Tspan15	A	T	10: 62,025,621 (GRCm39)	M197K	possibly damaging	Het
Upf1	C	T	8: 70,791,110 (GRCm39)	R544H	probably damaging	Het
Vegfb	T	C	19: 6,963,446 (GRCm39)	Y106C	probably damaging	Het
Vmn2r100	AAAACAGGAGTATTGATTGGAAAC	AAAAC	17: 19,743,681 (GRCm39)		probably null	Het
Vmn2r18	T	A	5: 151,485,730 (GRCm39)	Q588L	probably damaging	Het
Vmn2r66	T	C	7: 84,654,800 (GRCm39)	D503G	probably benign	Het
Zc3h15	T	C	2: 83,488,913 (GRCm39)	V161A	probably benign	Het

Other mutations in Rsad1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01104:Rsad1	APN	11	94,434,466 (GRCm39)	missense	possibly damaging	0.65
IGL01915:Rsad1	APN	11	94,439,803 (GRCm39)	splice site	probably null
R0271:Rsad1	UTSW	11	94,439,290 (GRCm39)	splice site	probably benign
R0619:Rsad1	UTSW	11	94,433,465 (GRCm39)	missense	probably damaging	1.00
R1147:Rsad1	UTSW	11	94,434,966 (GRCm39)	missense	probably damaging	1.00
R1147:Rsad1	UTSW	11	94,434,966 (GRCm39)	missense	probably damaging	1.00
R2069:Rsad1	UTSW	11	94,439,951 (GRCm39)	start gained	probably benign
R3831:Rsad1	UTSW	11	94,434,130 (GRCm39)	missense	probably benign	0.45
R3833:Rsad1	UTSW	11	94,434,130 (GRCm39)	missense	probably benign	0.45
R4467:Rsad1	UTSW	11	94,435,356 (GRCm39)	missense	probably benign
R4672:Rsad1	UTSW	11	94,434,444 (GRCm39)	missense	probably damaging	0.99
R5452:Rsad1	UTSW	11	94,434,515 (GRCm39)	missense	probably damaging	0.98
R6190:Rsad1	UTSW	11	94,439,062 (GRCm39)	missense	probably damaging	1.00
R6608:Rsad1	UTSW	11	94,433,435 (GRCm39)	missense	probably damaging	1.00
R6749:Rsad1	UTSW	11	94,434,166 (GRCm39)	missense	probably damaging	1.00
R7821:Rsad1	UTSW	11	94,435,288 (GRCm39)	missense	probably benign
R8818:Rsad1	UTSW	11	94,439,100 (GRCm39)	missense	probably benign	0.20
R8984:Rsad1	UTSW	11	94,439,010 (GRCm39)	missense	probably damaging	0.99
R9714:Rsad1	UTSW	11	94,435,298 (GRCm39)	missense	probably benign	0.00
X0024:Rsad1	UTSW	11	94,439,807 (GRCm39)	critical splice donor site	probably null
Z1177:Rsad1	UTSW	11	94,433,811 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- ATGTCCCCAGTACTGACCTCTG -3'
(R):5'- ACCGGAATAAGTGTTTGGCC -3'

Sequencing Primer
(F):5'- AGTACTGACCTCTGCACCC -3'
(R):5'- AACCTCAAGTCTGGTGCTTACTAG -3'

Posted On

2015-05-14