Mutagenetix > Incidental Mutation

Incidental Mutation 'R4156:Leng9'

315535

Institutional Source

Beutler Lab

Gene Symbol

Leng9

Ensembl Gene

ENSMUSG00000043432

Gene Name

leukocyte receptor cluster (LRC) member 9

Synonyms

9530024C23Rik, F630035L11Rik

MMRRC Submission

040862-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R4156 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

4151182-4152871 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 4152433 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 81 (D81G)

Ref Sequence

ENSEMBL: ENSMUSP00000061079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037472] [ENSMUST00000058358] [ENSMUST00000076831] [ENSMUST00000121270] [ENSMUST00000140410] [ENSMUST00000143825]

AlphaFold

Q8BTN6

Predicted Effect

probably benign
Transcript: ENSMUST00000037472

SMART Domains

Protein: ENSMUSP00000046465
Gene: ENSMUSG00000035545

Domain	Start	End	E-Value	Type
low complexity region	47	69	N/A	INTRINSIC
low complexity region	73	118	N/A	INTRINSIC
low complexity region	156	174	N/A	INTRINSIC
low complexity region	383	396	N/A	INTRINSIC
low complexity region	413	447	N/A	INTRINSIC
low complexity region	453	468	N/A	INTRINSIC
Pfam:SAC3_GANP	567	762	8.2e-66	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000058358
AA Change: D81G

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000061079
Gene: ENSMUSG00000043432
AA Change: D81G

Domain	Start	End	E-Value	Type
ZnF_C3H1	8	34	1.72e-4	SMART
Pfam:DUF504	77	128	1.9e-11	PFAM
Pfam:AKAP7_NLS	305	484	2.5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000076831

SMART Domains

Protein: ENSMUSP00000092508
Gene: ENSMUSG00000063838

Domain	Start	End	E-Value	Type
PBD	23	57	5.86e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121270

SMART Domains

Protein: ENSMUSP00000112428
Gene: ENSMUSG00000035545

Domain	Start	End	E-Value	Type
low complexity region	47	69	N/A	INTRINSIC
low complexity region	73	118	N/A	INTRINSIC
low complexity region	156	174	N/A	INTRINSIC
low complexity region	383	396	N/A	INTRINSIC
low complexity region	413	447	N/A	INTRINSIC
low complexity region	453	468	N/A	INTRINSIC
Pfam:SAC3_GANP	567	764	7.4e-67	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127723

Predicted Effect

probably benign
Transcript: ENSMUST00000140410

Predicted Effect

probably benign
Transcript: ENSMUST00000143825

SMART Domains

Protein: ENSMUSP00000117257
Gene: ENSMUSG00000063838

Domain	Start	End	E-Value	Type
PBD	23	57	5.86e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146434

Meta Mutation Damage Score

0.0806

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.6%

Validation Efficiency

96% (44/46)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410004B18Rik	T	A	3: 145,644,018 (GRCm39)	F69I	possibly damaging	Het
Acot12	C	T	13: 91,932,882 (GRCm39)	L552F	probably benign	Het
Aff4	T	A	11: 53,301,726 (GRCm39)		probably benign	Het
Aldh18a1	A	G	19: 40,539,725 (GRCm39)	V750A	probably damaging	Het
Anapc1	A	G	2: 128,469,149 (GRCm39)		probably benign	Het
Arl6ip1	AAAATAAATAAATAAATAAATAAATA	AAAATAAATAAATAAATAAATAAATAAATA	7: 117,721,122 (GRCm39)		probably benign	Het
Bcl11b	A	T	12: 107,883,684 (GRCm39)		probably null	Het
Ccpg1	C	A	9: 72,919,449 (GRCm39)	Q355K	probably benign	Het
Cdc42bpb	G	A	12: 111,260,573 (GRCm39)	P1702S	probably benign	Het
Ddx20	G	T	3: 105,586,249 (GRCm39)	Q699K	probably benign	Het
Ecd	A	G	14: 20,374,632 (GRCm39)	S503P	probably damaging	Het
Etaa1	C	T	11: 17,890,281 (GRCm39)	R860Q	probably damaging	Het
Ffar2	T	A	7: 30,519,093 (GRCm39)	Y149F	probably damaging	Het
Gamt	T	A	10: 80,096,558 (GRCm39)	R60*	probably null	Het
Gm6871	T	C	7: 41,195,510 (GRCm39)	N302S	probably damaging	Het
Hps3	A	G	3: 20,083,393 (GRCm39)	S135P	probably damaging	Het
Ifi203	T	A	1: 173,764,106 (GRCm39)	N122I	probably damaging	Het
Lrrc23	T	A	6: 124,747,804 (GRCm39)	K262*	probably null	Het
Morc2b	T	A	17: 33,357,401 (GRCm39)	T124S	probably benign	Het
Mroh1	G	A	15: 76,286,326 (GRCm39)		probably null	Het
Naxe	T	C	3: 87,964,011 (GRCm39)	K240R	probably benign	Het
Ncan	C	A	8: 70,562,727 (GRCm39)	E510D	possibly damaging	Het
Ndufs4	A	T	13: 114,444,390 (GRCm39)	S129R	probably benign	Het
Oog2	A	G	4: 143,920,523 (GRCm39)		probably benign	Het
Or5h18	A	T	16: 58,847,931 (GRCm39)	F113Y	probably damaging	Het
Or8h8	T	C	2: 86,753,222 (GRCm39)	Y218C	probably damaging	Het
Or8j3c	C	A	2: 86,253,544 (GRCm39)	V159L	possibly damaging	Het
Papola	G	A	12: 105,767,010 (GRCm39)		probably null	Het
Pasd1	T	C	X: 70,983,161 (GRCm39)	C378R	possibly damaging	Het
Plec	A	G	15: 76,056,453 (GRCm39)	S4517P	probably damaging	Het
Rpap1	C	T	2: 119,604,660 (GRCm39)	R416H	probably damaging	Het
Rpl31-ps17	C	T	12: 54,748,397 (GRCm39)		noncoding transcript	Het
Rxfp2	G	A	5: 149,975,020 (GRCm39)	V210I	probably benign	Het
Ryr3	T	C	2: 112,484,020 (GRCm39)	D3909G	probably damaging	Het
Spata31d1a	T	A	13: 59,852,861 (GRCm39)	K76N	possibly damaging	Het
Srgn	A	G	10: 62,333,613 (GRCm39)	F55L	possibly damaging	Het
Tmem54	G	A	4: 129,004,504 (GRCm39)	R151Q	probably damaging	Het
Tns1	T	A	1: 73,953,790 (GRCm39)	N1848Y	probably damaging	Het
Trim33	G	T	3: 103,217,630 (GRCm39)	V192L	possibly damaging	Het
Trpm5	G	T	7: 142,642,792 (GRCm39)	L52I	probably benign	Het
Uaca	A	G	9: 60,779,035 (GRCm39)	S1141G	probably benign	Het
Vmn1r63	T	C	7: 5,806,531 (GRCm39)	T34A	possibly damaging	Het
Vmn2r50	T	C	7: 9,774,309 (GRCm39)	K529R	probably benign	Het
Vmn2r9	T	C	5: 108,995,743 (GRCm39)	T302A	possibly damaging	Het
Ylpm1	T	C	12: 85,104,177 (GRCm39)		probably benign	Het
Zfp410	G	A	12: 84,374,206 (GRCm39)	R181H	probably damaging	Het

Other mutations in Leng9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01903:Leng9	APN	7	4,151,746 (GRCm39)	missense	probably damaging	1.00
IGL01970:Leng9	APN	7	4,151,326 (GRCm39)	missense	probably damaging	0.99
IGL02197:Leng9	APN	7	4,151,723 (GRCm39)	missense	probably damaging	1.00
IGL03013:Leng9	APN	7	4,151,737 (GRCm39)	missense	probably damaging	1.00
R1616:Leng9	UTSW	7	4,151,902 (GRCm39)	missense	probably benign	0.00
R2352:Leng9	UTSW	7	4,152,409 (GRCm39)	missense	probably damaging	1.00
R2419:Leng9	UTSW	7	4,151,626 (GRCm39)	missense	probably benign	0.32
R4832:Leng9	UTSW	7	4,152,029 (GRCm39)	missense	probably damaging	0.99
R4841:Leng9	UTSW	7	4,152,385 (GRCm39)	missense	probably damaging	1.00
R4842:Leng9	UTSW	7	4,152,385 (GRCm39)	missense	probably damaging	1.00
R7468:Leng9	UTSW	7	4,151,800 (GRCm39)	missense	probably benign
R7627:Leng9	UTSW	7	4,151,617 (GRCm39)	missense	probably damaging	1.00
R7679:Leng9	UTSW	7	4,152,659 (GRCm39)	missense	probably benign	0.11
R8885:Leng9	UTSW	7	4,151,774 (GRCm39)	missense	possibly damaging	0.95
R8951:Leng9	UTSW	7	4,152,782 (GRCm39)	unclassified	probably benign
R9062:Leng9	UTSW	7	4,151,666 (GRCm39)	missense	probably damaging	1.00
R9140:Leng9	UTSW	7	4,152,657 (GRCm39)	missense	probably benign
R9275:Leng9	UTSW	7	4,151,447 (GRCm39)	missense	probably benign	0.02
R9418:Leng9	UTSW	7	4,151,354 (GRCm39)	missense	probably benign	0.01
R9582:Leng9	UTSW	7	4,152,263 (GRCm39)	missense	probably damaging	1.00
X0058:Leng9	UTSW	7	4,152,382 (GRCm39)	missense	possibly damaging	0.56

Predicted Primers

PCR Primer
(F):5'- ATCAAGGATAGTGGGTCCCC -3'
(R):5'- CAGACAGAGACCGCTTAGTG -3'

Sequencing Primer
(F):5'- GCCACAGAGCCCGATCC -3'
(R):5'- CTTAGTGCGTGAAGGCCCATG -3'

Posted On

2015-05-14