Mutagenetix > Incidental Mutation

Incidental Mutation 'R4061:Vmn2r84'

315870

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r84

Ensembl Gene

ENSMUSG00000070601

Gene Name

vomeronasal 2, receptor 84

Synonyms

EG625068

MMRRC Submission

040852-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R4061 (G1)

Quality Score

194

Status

Not validated

Chromosome

Chromosomal Location

130221669-130230110 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 130221898 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Valine at position 774 (E774V)

Ref Sequence

ENSEMBL: ENSMUSP00000092079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094502]

AlphaFold

D3YWE3

Predicted Effect

probably damaging
Transcript: ENSMUST00000094502
AA Change: E774V

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000092079
Gene: ENSMUSG00000070601
AA Change: E774V

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	77	448	1.3e-27	PFAM
Pfam:NCD3G	508	561	6.9e-21	PFAM
Pfam:7tm_3	594	830	4.6e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

94% (51/54)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700019A02Rik	A	T	1: 53,197,928 (GRCm39)	L140Q	probably damaging	Het
Adam25	A	G	8: 41,206,819 (GRCm39)	I28M	possibly damaging	Het
Anks1b	A	G	10: 90,143,484 (GRCm39)	S464G	probably damaging	Het
AU040320	T	A	4: 126,729,488 (GRCm39)	M550K	probably damaging	Het
Cab39l	A	G	14: 59,737,056 (GRCm39)	K59E	possibly damaging	Het
Cdc5l	C	T	17: 45,721,816 (GRCm39)	A485T	probably benign	Het
Csmd1	A	G	8: 15,995,158 (GRCm39)	S2626P	probably benign	Het
Ctss	C	T	3: 95,450,345 (GRCm39)	R99W	probably benign	Het
Deptor	A	T	15: 55,072,177 (GRCm39)	M219L	probably benign	Het
Disp1	A	G	1: 182,869,264 (GRCm39)	V1052A	probably damaging	Het
Esyt3	C	T	9: 99,202,891 (GRCm39)	S504N	probably damaging	Het
Fat1	A	T	8: 45,478,518 (GRCm39)	E2521D	probably benign	Het
Folh1	T	A	7: 86,406,170 (GRCm39)	Y301F	possibly damaging	Het
Gm14443	G	A	2: 175,011,402 (GRCm39)	T348I	probably benign	Het
Gtpbp2	G	A	17: 46,478,253 (GRCm39)	R467H	probably damaging	Het
Hnrnpll	T	C	17: 80,340,201 (GRCm39)	H526R	probably benign	Het
Iars2	A	T	1: 185,035,583 (GRCm39)	H552Q	possibly damaging	Het
Il18r1	G	A	1: 40,514,096 (GRCm39)	V101I	probably benign	Het
Impdh2	A	G	9: 108,440,003 (GRCm39)	R182G	possibly damaging	Het
Krt12	A	T	11: 99,306,841 (GRCm39)	M487K	unknown	Het
Lmln	C	A	16: 32,886,761 (GRCm39)	Y89*	probably null	Het
Lrrc38	T	A	4: 143,077,076 (GRCm39)	L113Q	probably damaging	Het
Mast3	A	G	8: 71,233,838 (GRCm39)	V969A	probably damaging	Het
Muc5ac	A	G	7: 141,364,867 (GRCm39)	D1947G	possibly damaging	Het
Myh13	A	T	11: 67,221,715 (GRCm39)	I177F	possibly damaging	Het
Nfasc	T	C	1: 132,525,583 (GRCm39)	Y904C	probably damaging	Het
Obscn	G	A	11: 58,899,358 (GRCm39)	P1000S	probably damaging	Het
Or52n4	T	A	7: 104,293,680 (GRCm39)	K298*	probably null	Het
Or5t7	T	A	2: 86,507,162 (GRCm39)	I172F	probably damaging	Het
Otop2	G	A	11: 115,220,201 (GRCm39)	G347D	probably damaging	Het
Pclo	A	G	5: 14,590,580 (GRCm39)	E960G	unknown	Het
Plagl1	TGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCC	TGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCC	10: 13,004,515 (GRCm39)		probably benign	Het
Prl7a1	T	C	13: 27,819,832 (GRCm39)	I141V	possibly damaging	Het
Ptpn18	A	T	1: 34,512,011 (GRCm39)	H45L	possibly damaging	Het
Sbno1	A	T	5: 124,526,635 (GRCm39)	M960K	probably damaging	Het
Slx4ip	T	A	2: 136,846,937 (GRCm39)	S67R	probably benign	Het
Spata18	A	G	5: 73,828,509 (GRCm39)	K243E	probably damaging	Het
Tcp1	A	G	17: 13,139,750 (GRCm39)	Q265R	probably benign	Het
Tec	C	T	5: 72,980,752 (GRCm39)		probably benign	Het
Thbs4	A	G	13: 92,912,605 (GRCm39)		probably null	Het
Tln1	T	A	4: 43,549,177 (GRCm39)	Q635L	probably damaging	Het
Tshz2	A	G	2: 169,804,245 (GRCm39)		probably benign	Het
Uap1	T	C	1: 169,986,415 (GRCm39)	E189G	possibly damaging	Het
Usp21	G	A	1: 171,112,974 (GRCm39)		probably benign	Het
Vmn1r118	G	T	7: 20,645,933 (GRCm39)	Q114K	probably damaging	Het
Vmn1r38	A	T	6: 66,753,832 (GRCm39)	C95S	possibly damaging	Het
Vmn2r12	A	T	5: 109,240,058 (GRCm39)	N168K	possibly damaging	Het

Other mutations in Vmn2r84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Vmn2r84	APN	10	130,227,094 (GRCm39)	missense	possibly damaging	0.65
IGL01590:Vmn2r84	APN	10	130,221,964 (GRCm39)	missense	probably damaging	1.00
IGL01639:Vmn2r84	APN	10	130,225,141 (GRCm39)	nonsense	probably null
IGL01843:Vmn2r84	APN	10	130,222,148 (GRCm39)	missense	probably benign
IGL01911:Vmn2r84	APN	10	130,222,277 (GRCm39)	missense	probably damaging	0.99
IGL01937:Vmn2r84	APN	10	130,221,755 (GRCm39)	missense	probably damaging	1.00
IGL01977:Vmn2r84	APN	10	130,229,935 (GRCm39)	missense	probably benign	0.11
IGL02177:Vmn2r84	APN	10	130,227,881 (GRCm39)	missense	probably benign	0.00
IGL02291:Vmn2r84	APN	10	130,226,617 (GRCm39)	missense	probably damaging	1.00
IGL02590:Vmn2r84	APN	10	130,227,356 (GRCm39)	splice site	probably benign
IGL02727:Vmn2r84	APN	10	130,229,995 (GRCm39)	missense	possibly damaging	0.95
IGL02900:Vmn2r84	APN	10	130,223,861 (GRCm39)	splice site	probably benign
IGL03383:Vmn2r84	APN	10	130,222,556 (GRCm39)	missense	probably damaging	1.00
PIT4378001:Vmn2r84	UTSW	10	130,221,784 (GRCm39)	missense	probably damaging	1.00
R0076:Vmn2r84	UTSW	10	130,230,062 (GRCm39)	missense	probably damaging	1.00
R0089:Vmn2r84	UTSW	10	130,222,588 (GRCm39)	splice site	probably benign
R0153:Vmn2r84	UTSW	10	130,227,877 (GRCm39)	missense	probably benign	0.06
R0611:Vmn2r84	UTSW	10	130,221,991 (GRCm39)	missense	probably damaging	1.00
R0883:Vmn2r84	UTSW	10	130,226,984 (GRCm39)	missense	probably damaging	0.99
R1237:Vmn2r84	UTSW	10	130,223,725 (GRCm39)	splice site	probably null
R1295:Vmn2r84	UTSW	10	130,225,008 (GRCm39)	missense	probably benign	0.12
R1401:Vmn2r84	UTSW	10	130,227,859 (GRCm39)	missense	possibly damaging	0.89
R1521:Vmn2r84	UTSW	10	130,225,137 (GRCm39)	missense	probably benign	0.10
R1590:Vmn2r84	UTSW	10	130,227,349 (GRCm39)	critical splice acceptor site	probably null
R1710:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R1891:Vmn2r84	UTSW	10	130,221,938 (GRCm39)	missense	possibly damaging	0.78
R1956:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1957:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1962:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
R1994:Vmn2r84	UTSW	10	130,221,878 (GRCm39)	missense	probably damaging	1.00
R2124:Vmn2r84	UTSW	10	130,227,100 (GRCm39)	missense	probably damaging	0.99
R2409:Vmn2r84	UTSW	10	130,227,940 (GRCm39)	missense	probably damaging	0.99
R2474:Vmn2r84	UTSW	10	130,222,392 (GRCm39)	missense	possibly damaging	0.50
R2851:Vmn2r84	UTSW	10	130,230,036 (GRCm39)	missense	probably benign	0.05
R3508:Vmn2r84	UTSW	10	130,226,777 (GRCm39)	missense	probably damaging	1.00
R3792:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R4051:Vmn2r84	UTSW	10	130,226,767 (GRCm39)	missense	probably damaging	1.00
R4091:Vmn2r84	UTSW	10	130,227,238 (GRCm39)	missense	probably damaging	1.00
R4190:Vmn2r84	UTSW	10	130,227,163 (GRCm39)	nonsense	probably null
R4520:Vmn2r84	UTSW	10	130,222,391 (GRCm39)	missense	probably damaging	1.00
R4584:Vmn2r84	UTSW	10	130,226,582 (GRCm39)	missense	probably benign	0.00
R4588:Vmn2r84	UTSW	10	130,221,809 (GRCm39)	missense	probably damaging	0.98
R4655:Vmn2r84	UTSW	10	130,229,973 (GRCm39)	nonsense	probably null
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R5022:Vmn2r84	UTSW	10	130,222,417 (GRCm39)	missense	probably damaging	1.00
R5146:Vmn2r84	UTSW	10	130,221,971 (GRCm39)	missense	probably damaging	1.00
R5237:Vmn2r84	UTSW	10	130,221,863 (GRCm39)	missense	probably damaging	0.99
R5695:Vmn2r84	UTSW	10	130,225,064 (GRCm39)	missense	probably benign	0.12
R5793:Vmn2r84	UTSW	10	130,221,754 (GRCm39)	missense	probably damaging	0.99
R6210:Vmn2r84	UTSW	10	130,222,114 (GRCm39)	missense	probably damaging	1.00
R6286:Vmn2r84	UTSW	10	130,226,737 (GRCm39)	missense	possibly damaging	0.65
R6580:Vmn2r84	UTSW	10	130,225,110 (GRCm39)	missense	possibly damaging	0.93
R6607:Vmn2r84	UTSW	10	130,226,731 (GRCm39)	missense	possibly damaging	0.87
R6818:Vmn2r84	UTSW	10	130,222,147 (GRCm39)	missense	probably benign	0.09
R6956:Vmn2r84	UTSW	10	130,225,136 (GRCm39)	missense	probably damaging	0.98
R6994:Vmn2r84	UTSW	10	130,226,876 (GRCm39)	missense	possibly damaging	0.90
R7075:Vmn2r84	UTSW	10	130,226,941 (GRCm39)	missense	probably damaging	0.99
R7225:Vmn2r84	UTSW	10	130,222,552 (GRCm39)	missense	probably damaging	0.99
R7252:Vmn2r84	UTSW	10	130,222,279 (GRCm39)	missense	probably damaging	1.00
R7263:Vmn2r84	UTSW	10	130,225,077 (GRCm39)	missense	probably damaging	1.00
R7297:Vmn2r84	UTSW	10	130,227,119 (GRCm39)	missense	probably benign	0.19
R7439:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7441:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7857:Vmn2r84	UTSW	10	130,226,738 (GRCm39)	missense	probably benign	0.00
R8263:Vmn2r84	UTSW	10	130,227,037 (GRCm39)	missense	probably damaging	1.00
R8555:Vmn2r84	UTSW	10	130,230,100 (GRCm39)	missense	probably benign	0.28
R8766:Vmn2r84	UTSW	10	130,222,241 (GRCm39)	missense	probably damaging	0.98
R8821:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8831:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8970:Vmn2r84	UTSW	10	130,222,244 (GRCm39)	missense	probably damaging	0.98
R9164:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R9190:Vmn2r84	UTSW	10	130,226,843 (GRCm39)	missense	probably benign	0.03
R9261:Vmn2r84	UTSW	10	130,229,976 (GRCm39)	missense	probably benign	0.00
R9310:Vmn2r84	UTSW	10	130,227,993 (GRCm39)	missense	possibly damaging	0.81
R9434:Vmn2r84	UTSW	10	130,221,745 (GRCm39)	missense	possibly damaging	0.65
R9613:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r84	UTSW	10	130,227,771 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGGACAGTGCTATCCTGTTTG -3'
(R):5'- AACATCTCCTCCCTTTATTGACAGAG -3'

Sequencing Primer
(F):5'- GACAGTGCTATCCTGTTTGTAAGTCC -3'
(R):5'- TCCTCCCTTTATTGACAGAGATATAC -3'

Posted On

2015-05-15