|Institutional Source||Beutler Lab|
|Gene Name||argonaute RISC catalytic subunit 4|
|Synonyms||argonaute 4, Eif2c4, 5730550L01Rik|
|Is this an essential gene?||Possibly essential (E-score: 0.613)|
|Stock #||R4063 (G1)|
|Chromosomal Location||126489541-126533472 bp(-) (GRCm38)|
|Type of Mutation||intron|
|DNA Base Change (assembly)||A to T at 126515862 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000081312 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000084289]|
|Predicted Effect||probably benign
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.058|
|Coding Region Coverage||
|Validation Efficiency||97% (59/61)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Argonaute family of proteins which contain PAZ and PIWI domains and play an integral role in RNA interference and short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of related family members. [provided by RefSeq, Mar 2017]
PHENOTYPE: Male mice homozygous for a null mutation display oligozoospermia, decreased testis weight, premature entry into meiosis and disruption of sex body formation. However both males and females are fertile. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ago4||
(F):5'- TAGCTTCCTCTGTTATCAAACGGA -3'
(R):5'- AGAATTCTTTCCAGAGTAATGCAC -3'
(F):5'- TGGAACTCACTCTGTAGACCAGG -3'
(R):5'- GAGCTCACTCTATAGATCAGGCTG -3'