Mutagenetix > Incidental Mutation

Incidental Mutation 'R2061:Akap9'

316237

Institutional Source

Beutler Lab

Gene Symbol

Akap9

Ensembl Gene

ENSMUSG00000040407

Gene Name

A kinase anchor protein 9

Synonyms

mei2-5, repro12, AKAP450, G1-448-15, 5730481H23Rik

MMRRC Submission

040066-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.436) question?

Stock #

R2061 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

3977410-4130204 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 4011010 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 571 (V571E)

Ref Sequence

ENSEMBL: ENSMUSP00000046129 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044492] [ENSMUST00000143365]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000044492
AA Change: V571E

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000046129
Gene: ENSMUSG00000040407
AA Change: V571E

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	98	115	N/A	INTRINSIC
Blast:HPT	126	197	6e-21	BLAST
low complexity region	237	249	N/A	INTRINSIC
low complexity region	297	315	N/A	INTRINSIC
coiled coil region	404	593	N/A	INTRINSIC
coiled coil region	622	756	N/A	INTRINSIC
coiled coil region	777	843	N/A	INTRINSIC
coiled coil region	888	958	N/A	INTRINSIC
low complexity region	982	997	N/A	INTRINSIC
coiled coil region	1037	1065	N/A	INTRINSIC
low complexity region	1233	1246	N/A	INTRINSIC
internal_repeat_2	1247	1312	7.75e-5	PROSPERO
internal_repeat_1	1377	1485	2.63e-5	PROSPERO
coiled coil region	1522	1589	N/A	INTRINSIC
coiled coil region	1789	2107	N/A	INTRINSIC
coiled coil region	2132	2318	N/A	INTRINSIC
internal_repeat_1	2322	2445	2.63e-5	PROSPERO
coiled coil region	2455	2494	N/A	INTRINSIC
low complexity region	2587	2598	N/A	INTRINSIC
low complexity region	2627	2640	N/A	INTRINSIC
internal_repeat_2	2934	2997	7.75e-5	PROSPERO
low complexity region	3000	3016	N/A	INTRINSIC
coiled coil region	3109	3307	N/A	INTRINSIC
coiled coil region	3455	3493	N/A	INTRINSIC
coiled coil region	3521	3556	N/A	INTRINSIC
Pfam:PACT_coil_coil	3576	3657	1.2e-27	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000140977

Predicted Effect

probably benign
Transcript: ENSMUST00000143365

SMART Domains

Protein: ENSMUSP00000117209
Gene: ENSMUSG00000040407

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	122	133	N/A	INTRINSIC
Blast:HPT	144	215	9e-22	BLAST
low complexity region	255	267	N/A	INTRINSIC
low complexity region	315	333	N/A	INTRINSIC
coiled coil region	422	571	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177448

Meta Mutation Damage Score

0.1774

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

99% (124/125)

MGI Phenotype

PHENOTYPE: Mice homozygous for a chemically induced allele exhibit male infertily with abnormal spermatogenesis and Sertoli maturation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 122 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4gnt	T	C	9: 99,502,412 (GRCm39)	S191P	probably damaging	Het
Ak2	C	T	4: 128,901,990 (GRCm39)	A221V	probably damaging	Het
Amph	G	T	13: 19,309,205 (GRCm39)	E428*	probably null	Het
Armh3	C	T	19: 45,967,106 (GRCm39)	R12Q	probably damaging	Het
Arnt2	T	A	7: 83,993,078 (GRCm39)	D154V	probably damaging	Het
Arrdc1	G	A	2: 24,816,364 (GRCm39)	Q202*	probably null	Het
Ate1	A	G	7: 130,112,643 (GRCm39)	C72R	probably damaging	Het
Atox1	A	G	11: 55,345,724 (GRCm39)	V22A	possibly damaging	Het
Bbs12	A	T	3: 37,373,215 (GRCm39)	M3L	probably damaging	Het
Bfsp1	A	G	2: 143,704,598 (GRCm39)	V85A	probably benign	Het
Bltp2	T	A	11: 78,159,575 (GRCm39)	C541*	probably null	Het
Caskin2	C	A	11: 115,694,456 (GRCm39)	V382F	probably benign	Het
Ccdc39	T	A	3: 33,874,045 (GRCm39)	M596L	probably damaging	Het
Cd22	C	T	7: 30,569,530 (GRCm39)	V529M	probably damaging	Het
Cd22	A	C	7: 30,575,581 (GRCm39)	Y154D	probably benign	Het
Cdadc1	T	A	14: 59,818,783 (GRCm39)	E348D	probably damaging	Het
Cdhr2	T	C	13: 54,868,631 (GRCm39)	V531A	probably damaging	Het
Cdk11b	A	G	4: 155,726,061 (GRCm39)		probably benign	Het
Cebpa	G	T	7: 34,818,947 (GRCm39)	R35L	probably damaging	Het
Chat	T	C	14: 32,168,830 (GRCm39)	N235S	probably benign	Het
Col12a1	T	A	9: 79,524,987 (GRCm39)	I2725F	possibly damaging	Het
Cracr2b	A	G	7: 141,045,193 (GRCm39)	E231G	probably damaging	Het
Cryaa	G	T	17: 31,900,029 (GRCm39)	A151S	probably benign	Het
Cspg4b	T	A	13: 113,454,628 (GRCm39)	W225R	probably damaging	Het
Dab1	A	T	4: 104,535,938 (GRCm39)	I116F	probably damaging	Het
Ddx43	C	A	9: 78,303,386 (GRCm39)	N75K	probably benign	Het
Dmbt1	T	G	7: 130,700,863 (GRCm39)	C1014G	possibly damaging	Het
Dner	C	A	1: 84,383,710 (GRCm39)	C558F	probably damaging	Het
Dsc2	A	G	18: 20,165,456 (GRCm39)	V839A	possibly damaging	Het
Dsg3	C	T	18: 20,660,794 (GRCm39)	R378*	probably null	Het
Ecpas	G	T	4: 58,824,270 (GRCm39)	P1116T	probably damaging	Het
Emilin1	C	T	5: 31,075,082 (GRCm39)	P441L	possibly damaging	Het
Epha6	T	C	16: 59,476,160 (GRCm39)	M1069V	probably damaging	Het
F930017D23Rik	A	C	10: 43,480,416 (GRCm39)		noncoding transcript	Het
Faf1	A	T	4: 109,568,005 (GRCm39)	N22Y	probably damaging	Het
Flrt3	A	T	2: 140,503,373 (GRCm39)	V85E	probably damaging	Het
Gadl1	T	A	9: 115,770,448 (GRCm39)	I87N	probably damaging	Het
Galnt14	A	G	17: 73,819,148 (GRCm39)	F314S	probably damaging	Het
Gba2	A	T	4: 43,574,029 (GRCm39)	Y141*	probably null	Het
Gdap1	A	T	1: 17,215,689 (GRCm39)		probably benign	Het
Gfod1	A	T	13: 43,456,719 (GRCm39)		probably null	Het
Gm14295	C	T	2: 176,502,474 (GRCm39)	R655*	probably null	Het
Gm4353	A	G	7: 115,682,934 (GRCm39)	S216P	probably damaging	Het
Gm6605	T	A	7: 38,147,706 (GRCm39)		noncoding transcript	Het
Hectd1	A	T	12: 51,841,227 (GRCm39)	D634E	probably damaging	Het
Helz2	A	G	2: 180,882,337 (GRCm39)	I152T	probably damaging	Het
Hivep1	A	G	13: 42,313,600 (GRCm39)	K1947E	possibly damaging	Het
Ifrd1	A	T	12: 40,263,244 (GRCm39)	F144L	probably benign	Het
Inhca	T	C	9: 103,145,513 (GRCm39)	M395V	probably benign	Het
Itgae	A	T	11: 73,009,448 (GRCm39)	Q544L	probably benign	Het
Jmjd1c	A	T	10: 67,054,205 (GRCm39)	E323D	probably damaging	Het
Kdm5a	G	T	6: 120,358,578 (GRCm39)	R207L	probably benign	Het
Kif5b	C	T	18: 6,226,377 (GRCm39)		probably null	Het
Lbp	T	C	2: 158,166,499 (GRCm39)	V351A	probably benign	Het
Lss	A	T	10: 76,381,932 (GRCm39)		probably null	Het
Madd	A	C	2: 90,991,831 (GRCm39)		probably benign	Het
Map6	G	A	7: 98,966,679 (GRCm39)	V503I	probably damaging	Het
Mark1	A	G	1: 184,660,260 (GRCm39)	L22P	probably damaging	Het
Mcfd2	T	C	17: 87,563,404 (GRCm39)	N130D	probably damaging	Het
Mcm3ap	A	G	10: 76,305,902 (GRCm39)	N5S	probably benign	Het
Mdm4	A	T	1: 132,940,389 (GRCm39)	F48I	probably damaging	Het
Mga	A	T	2: 119,795,461 (GRCm39)		probably benign	Het
Mknk2	A	T	10: 80,507,391 (GRCm39)		probably null	Het
Mmp15	T	C	8: 96,097,407 (GRCm39)	Y459H	possibly damaging	Het
Mthfd1l	A	G	10: 4,053,288 (GRCm39)	K879R	probably benign	Het
Mycbp2	T	C	14: 103,524,696 (GRCm39)	K655E	probably damaging	Het
Nasp	T	C	4: 116,468,323 (GRCm39)	N221D	probably benign	Het
Ndc80	T	C	17: 71,821,213 (GRCm39)	E245G	probably benign	Het
Nmral1	C	T	16: 4,534,193 (GRCm39)	E83K	probably damaging	Het
Noa1	T	A	5: 77,452,034 (GRCm39)	Q550L	possibly damaging	Het
Nutm1	A	T	2: 112,086,097 (GRCm39)	Y211*	probably null	Het
Or10n1	G	T	9: 39,525,071 (GRCm39)	M69I	probably benign	Het
Or10v1	T	A	19: 11,873,921 (GRCm39)	Y179N	probably damaging	Het
Or2ag18	A	C	7: 106,404,975 (GRCm39)	H231Q	probably benign	Het
Or4l1	T	C	14: 50,166,478 (GRCm39)	I174M	possibly damaging	Het
Or5b118	A	G	19: 13,448,605 (GRCm39)	I90M	probably damaging	Het
Otoa	T	C	7: 120,730,551 (GRCm39)	F584L	probably damaging	Het
Palb2	G	A	7: 121,723,748 (GRCm39)	T304I	possibly damaging	Het
Pcolce2	A	T	9: 95,552,229 (GRCm39)	M121L	probably benign	Het
Pcsk5	T	C	19: 17,432,236 (GRCm39)	T1460A	probably benign	Het
Pdzrn4	A	T	15: 92,668,041 (GRCm39)	D731V	probably damaging	Het
Pex11b	C	A	3: 96,543,037 (GRCm39)	Q12K	possibly damaging	Het
Pigw	G	C	11: 84,768,136 (GRCm39)	Q398E	probably benign	Het
Pkd1	A	G	17: 24,788,888 (GRCm39)	E882G	possibly damaging	Het
Pkhd1	A	T	1: 20,683,036 (GRCm39)	N55K	possibly damaging	Het
Plch2	A	T	4: 155,127,298 (GRCm39)		probably benign	Het
Plcl1	T	A	1: 55,790,504 (GRCm39)	L1058Q	probably benign	Het
Ppfia2	T	C	10: 106,673,190 (GRCm39)	S511P	possibly damaging	Het
Ppfibp2	T	C	7: 107,338,437 (GRCm39)	L676P	probably damaging	Het
Ppp6c	T	G	2: 39,116,186 (GRCm39)	D23A	probably damaging	Het
Prss30	G	A	17: 24,193,642 (GRCm39)		probably benign	Het
Ptprz1	T	C	6: 23,049,674 (GRCm39)		probably null	Het
Rec114	T	A	9: 58,560,188 (GRCm39)		probably benign	Het
Ryr2	T	C	13: 11,680,764 (GRCm39)		probably null	Het
Ryr3	A	T	2: 112,493,349 (GRCm39)	I3715N	possibly damaging	Het
Scin	T	C	12: 40,130,947 (GRCm39)	Y322C	probably damaging	Het
Scn3a	A	G	2: 65,291,652 (GRCm39)	V1698A	probably damaging	Het
Scn5a	G	A	9: 119,314,717 (GRCm39)	S1996L	probably damaging	Het
Serpinb2	A	G	1: 107,450,525 (GRCm39)	K174R	possibly damaging	Het
Slc16a4	T	C	3: 107,208,027 (GRCm39)	I179T	probably benign	Het
Slc35b1	T	G	11: 95,276,718 (GRCm39)	F102V	possibly damaging	Het
Slc66a2	G	T	18: 80,334,930 (GRCm39)	A232S	probably benign	Het
Slc6a15	A	G	10: 103,245,595 (GRCm39)	D526G	probably benign	Het
Spata16	A	G	3: 26,978,519 (GRCm39)	D495G	probably damaging	Het
Stk11ip	G	A	1: 75,506,228 (GRCm39)	E583K	possibly damaging	Het
Stk-ps1	T	G	17: 36,709,044 (GRCm39)		noncoding transcript	Het
Sufu	T	A	19: 46,385,651 (GRCm39)	I37N	probably damaging	Het
Tacr1	C	T	6: 82,469,535 (GRCm39)	P140S	probably damaging	Het
Tas1r3	G	A	4: 155,944,927 (GRCm39)	R765C	probably damaging	Het
Tenm3	C	T	8: 48,795,291 (GRCm39)		probably null	Het
Tex36	A	T	7: 133,196,952 (GRCm39)	I55N	probably damaging	Het
Tmem150c	T	C	5: 100,227,887 (GRCm39)	Y192C	probably damaging	Het
Tmem237	A	G	1: 59,159,445 (GRCm39)		probably benign	Het
Trim11	A	G	11: 58,872,889 (GRCm39)	E191G	probably damaging	Het
Ttll3	C	T	6: 113,386,003 (GRCm39)	A612V	possibly damaging	Het
Ttn	G	A	2: 76,807,466 (GRCm39)	A89V	probably damaging	Het
Usp37	A	G	1: 74,507,431 (GRCm39)	F529L	probably damaging	Het
Vmn1r217	A	C	13: 23,298,698 (GRCm39)	V68G	probably benign	Het
Vwf	T	C	6: 125,568,151 (GRCm39)	S349P	probably damaging	Het
Wt1	G	A	2: 104,961,502 (GRCm39)		probably null	Het
Zcchc7	T	A	4: 44,895,838 (GRCm39)	L262H	probably damaging	Het
Zfp873	A	G	10: 81,895,991 (GRCm39)	S241G	probably benign	Het

Other mutations in Akap9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00478:Akap9	APN	5	4,096,639 (GRCm39)	missense	probably damaging	0.97
IGL00642:Akap9	APN	5	4,010,842 (GRCm39)	missense	probably damaging	0.99
IGL00786:Akap9	APN	5	4,120,522 (GRCm39)	missense	probably damaging	1.00
IGL00788:Akap9	APN	5	4,110,480 (GRCm39)	missense	probably damaging	1.00
IGL00969:Akap9	APN	5	4,051,550 (GRCm39)	missense	probably benign
IGL01014:Akap9	APN	5	4,018,683 (GRCm39)	missense	probably benign	0.41
IGL01302:Akap9	APN	5	4,020,711 (GRCm39)	missense	probably benign	0.27
IGL01610:Akap9	APN	5	4,082,839 (GRCm39)	missense	possibly damaging	0.95
IGL01620:Akap9	APN	5	4,010,218 (GRCm39)	missense	probably benign	0.11
IGL01862:Akap9	APN	5	4,115,856 (GRCm39)	missense	probably damaging	0.99
IGL01862:Akap9	APN	5	4,001,705 (GRCm39)	missense	probably damaging	0.99
IGL02151:Akap9	APN	5	4,082,728 (GRCm39)	nonsense	probably null
IGL02635:Akap9	APN	5	4,120,500 (GRCm39)	missense	possibly damaging	0.59
IGL02858:Akap9	APN	5	4,119,130 (GRCm39)	missense	possibly damaging	0.88
IGL02967:Akap9	APN	5	4,026,164 (GRCm39)	missense	probably benign	0.07
IGL03064:Akap9	APN	5	4,018,755 (GRCm39)	missense	probably damaging	1.00
IGL03289:Akap9	APN	5	4,127,261 (GRCm39)	missense	probably damaging	1.00
Andy	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
blimey	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
hoarder	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
marinarum	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
miser	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
naviculus	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
thrifty	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
wee_one	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
FR4449:Akap9	UTSW	5	4,031,214 (GRCm39)	unclassified	probably benign
PIT1430001:Akap9	UTSW	5	4,079,849 (GRCm39)	missense	probably damaging	1.00
PIT4366001:Akap9	UTSW	5	4,096,221 (GRCm39)	missense	probably benign	0.24
R0088:Akap9	UTSW	5	4,011,946 (GRCm39)	missense	probably benign	0.22
R0309:Akap9	UTSW	5	4,119,038 (GRCm39)	missense	probably benign	0.01
R0387:Akap9	UTSW	5	4,001,678 (GRCm39)	splice site	probably benign
R0440:Akap9	UTSW	5	4,114,569 (GRCm39)	missense	probably damaging	0.99
R0441:Akap9	UTSW	5	4,011,714 (GRCm39)	missense	probably benign	0.15
R0491:Akap9	UTSW	5	4,022,851 (GRCm39)	unclassified	probably benign
R0501:Akap9	UTSW	5	4,020,685 (GRCm39)	missense	probably damaging	1.00
R0507:Akap9	UTSW	5	4,119,043 (GRCm39)	missense	probably benign	0.41
R0544:Akap9	UTSW	5	4,119,185 (GRCm39)	missense	probably benign	0.22
R0581:Akap9	UTSW	5	4,100,620 (GRCm39)	missense	probably benign	0.03
R0611:Akap9	UTSW	5	4,004,870 (GRCm39)	missense	probably benign	0.00
R0620:Akap9	UTSW	5	4,114,136 (GRCm39)	missense	probably damaging	0.98
R0639:Akap9	UTSW	5	4,110,318 (GRCm39)	missense	probably damaging	1.00
R0932:Akap9	UTSW	5	4,096,492 (GRCm39)	missense	possibly damaging	0.77
R0944:Akap9	UTSW	5	4,114,742 (GRCm39)	splice site	probably null
R1101:Akap9	UTSW	5	4,096,205 (GRCm39)	missense	probably benign	0.00
R1159:Akap9	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
R1170:Akap9	UTSW	5	4,105,671 (GRCm39)	missense	probably benign
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1453:Akap9	UTSW	5	4,025,614 (GRCm39)	splice site	probably null
R1551:Akap9	UTSW	5	4,119,174 (GRCm39)	missense	probably benign	0.02
R1608:Akap9	UTSW	5	4,011,783 (GRCm39)	missense	probably damaging	1.00
R1652:Akap9	UTSW	5	4,127,210 (GRCm39)	missense	probably damaging	1.00
R1659:Akap9	UTSW	5	4,114,633 (GRCm39)	missense	probably damaging	1.00
R1713:Akap9	UTSW	5	4,089,345 (GRCm39)	critical splice donor site	probably null
R1719:Akap9	UTSW	5	4,007,645 (GRCm39)	nonsense	probably null
R1720:Akap9	UTSW	5	4,022,791 (GRCm39)	missense	possibly damaging	0.63
R1757:Akap9	UTSW	5	4,051,667 (GRCm39)	missense	probably benign	0.41
R1872:Akap9	UTSW	5	4,051,406 (GRCm39)	missense	probably damaging	1.00
R1876:Akap9	UTSW	5	4,011,809 (GRCm39)	missense	probably benign	0.28
R1881:Akap9	UTSW	5	4,100,173 (GRCm39)	missense	probably benign
R1950:Akap9	UTSW	5	4,010,677 (GRCm39)	missense	probably damaging	1.00
R1980:Akap9	UTSW	5	4,022,771 (GRCm39)	missense	probably damaging	0.99
R1993:Akap9	UTSW	5	4,088,520 (GRCm39)	splice site	probably null
R2008:Akap9	UTSW	5	4,010,131 (GRCm39)	missense	possibly damaging	0.47
R2020:Akap9	UTSW	5	4,011,967 (GRCm39)	missense	probably damaging	1.00
R2051:Akap9	UTSW	5	4,025,685 (GRCm39)	nonsense	probably null
R2109:Akap9	UTSW	5	4,094,847 (GRCm39)	missense	possibly damaging	0.47
R2135:Akap9	UTSW	5	4,114,509 (GRCm39)	missense	probably damaging	1.00
R2225:Akap9	UTSW	5	4,127,271 (GRCm39)	missense	probably damaging	0.96
R2232:Akap9	UTSW	5	4,096,603 (GRCm39)	missense	probably damaging	1.00
R2424:Akap9	UTSW	5	4,115,279 (GRCm39)	missense	probably damaging	0.97
R2483:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R2879:Akap9	UTSW	5	4,026,353 (GRCm39)	intron	probably benign
R3622:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3623:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3624:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3722:Akap9	UTSW	5	4,120,351 (GRCm39)	missense	probably damaging	1.00
R3806:Akap9	UTSW	5	4,004,410 (GRCm39)	missense	probably benign	0.00
R3919:Akap9	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
R4023:Akap9	UTSW	5	4,042,077 (GRCm39)	missense	possibly damaging	0.66
R4093:Akap9	UTSW	5	4,093,996 (GRCm39)	missense	probably damaging	0.99
R4434:Akap9	UTSW	5	4,082,708 (GRCm39)	missense	probably damaging	0.99
R4529:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4530:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4532:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4533:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4585:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4586:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4655:Akap9	UTSW	5	4,096,403 (GRCm39)	missense	probably benign	0.14
R4676:Akap9	UTSW	5	4,114,515 (GRCm39)	nonsense	probably null
R4676:Akap9	UTSW	5	4,082,774 (GRCm39)	missense	probably damaging	1.00
R4724:Akap9	UTSW	5	4,105,339 (GRCm39)	missense	probably benign
R4731:Akap9	UTSW	5	4,012,266 (GRCm39)	missense	possibly damaging	0.54
R4732:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4733:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4743:Akap9	UTSW	5	4,011,013 (GRCm39)	missense	probably damaging	1.00
R4749:Akap9	UTSW	5	4,018,737 (GRCm39)	missense	probably benign	0.41
R4756:Akap9	UTSW	5	4,051,418 (GRCm39)	missense	probably damaging	0.99
R4757:Akap9	UTSW	5	4,058,382 (GRCm39)	missense	probably damaging	1.00
R4860:Akap9	UTSW	5	4,084,916 (GRCm39)	intron	probably benign
R4937:Akap9	UTSW	5	4,100,145 (GRCm39)	splice site	probably null
R4960:Akap9	UTSW	5	4,007,664 (GRCm39)	missense	probably benign	0.15
R4974:Akap9	UTSW	5	4,011,466 (GRCm39)	missense	possibly damaging	0.81
R5101:Akap9	UTSW	5	4,051,748 (GRCm39)	missense	probably damaging	0.96
R5160:Akap9	UTSW	5	4,080,007 (GRCm39)	missense	probably damaging	1.00
R5200:Akap9	UTSW	5	4,010,734 (GRCm39)	missense	probably benign	0.00
R5245:Akap9	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
R5293:Akap9	UTSW	5	3,998,687 (GRCm39)	missense	probably damaging	0.99
R5408:Akap9	UTSW	5	4,108,458 (GRCm39)	missense	possibly damaging	0.84
R5507:Akap9	UTSW	5	4,018,683 (GRCm39)	missense	probably benign	0.41
R5517:Akap9	UTSW	5	4,051,665 (GRCm39)	missense	possibly damaging	0.76
R5579:Akap9	UTSW	5	4,114,714 (GRCm39)	missense	possibly damaging	0.93
R5619:Akap9	UTSW	5	4,004,760 (GRCm39)	intron	probably benign
R5645:Akap9	UTSW	5	4,100,590 (GRCm39)	missense	probably benign	0.09
R5669:Akap9	UTSW	5	4,100,540 (GRCm39)	nonsense	probably null
R5686:Akap9	UTSW	5	4,021,926 (GRCm39)	missense	probably benign	0.00
R5697:Akap9	UTSW	5	4,010,170 (GRCm39)	missense	possibly damaging	0.92
R5821:Akap9	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
R5875:Akap9	UTSW	5	4,127,285 (GRCm39)	missense	probably benign	0.01
R5897:Akap9	UTSW	5	4,127,904 (GRCm39)	missense	probably benign	0.23
R5999:Akap9	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
R6025:Akap9	UTSW	5	4,082,801 (GRCm39)	missense	probably damaging	1.00
R6078:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6138:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6225:Akap9	UTSW	5	4,012,105 (GRCm39)	missense	probably damaging	1.00
R6243:Akap9	UTSW	5	4,115,000 (GRCm39)	splice site	probably null
R6326:Akap9	UTSW	5	4,012,061 (GRCm39)	missense	probably damaging	1.00
R6564:Akap9	UTSW	5	4,078,491 (GRCm39)	missense	probably damaging	0.98
R6617:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6625:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6632:Akap9	UTSW	5	4,063,842 (GRCm39)	splice site	probably null
R6677:Akap9	UTSW	5	4,079,869 (GRCm39)	missense	probably benign	0.21
R6717:Akap9	UTSW	5	4,114,086 (GRCm39)	missense	probably damaging	1.00
R6893:Akap9	UTSW	5	4,011,709 (GRCm39)	missense	probably benign	0.32
R6915:Akap9	UTSW	5	4,010,551 (GRCm39)	missense	probably benign	0.03
R6938:Akap9	UTSW	5	4,096,628 (GRCm39)	missense	possibly damaging	0.91
R6972:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6973:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6993:Akap9	UTSW	5	4,115,866 (GRCm39)	missense	possibly damaging	0.65
R7032:Akap9	UTSW	5	4,004,896 (GRCm39)	missense	probably benign
R7164:Akap9	UTSW	5	4,110,364 (GRCm39)	missense	probably damaging	0.96
R7170:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7192:Akap9	UTSW	5	4,055,723 (GRCm39)	splice site	probably null
R7284:Akap9	UTSW	5	4,006,246 (GRCm39)	missense	probably damaging	1.00
R7299:Akap9	UTSW	5	4,082,696 (GRCm39)	missense	probably damaging	1.00
R7313:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7326:Akap9	UTSW	5	4,095,930 (GRCm39)	missense	possibly damaging	0.47
R7343:Akap9	UTSW	5	4,096,364 (GRCm39)	missense	probably damaging	0.99
R7455:Akap9	UTSW	5	4,022,792 (GRCm39)	missense	probably benign	0.03
R7482:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7489:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7525:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7528:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7576:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7577:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7578:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7610:Akap9	UTSW	5	4,007,677 (GRCm39)	missense	possibly damaging	0.95
R7658:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7754:Akap9	UTSW	5	4,096,736 (GRCm39)	missense	probably benign	0.03
R7818:Akap9	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
R7979:Akap9	UTSW	5	4,100,381 (GRCm39)	missense	probably benign
R7991:Akap9	UTSW	5	4,114,949 (GRCm39)	splice site	probably null
R8036:Akap9	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
R8054:Akap9	UTSW	5	4,088,707 (GRCm39)	critical splice donor site	probably null
R8116:Akap9	UTSW	5	4,111,183 (GRCm39)	missense	probably benign	0.04
R8150:Akap9	UTSW	5	4,011,982 (GRCm39)	missense	probably damaging	1.00
R8234:Akap9	UTSW	5	4,094,845 (GRCm39)	missense	probably benign	0.18
R8348:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8365:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8366:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8448:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8466:Akap9	UTSW	5	4,088,659 (GRCm39)	missense	probably damaging	1.00
R8772:Akap9	UTSW	5	4,096,255 (GRCm39)	missense	probably damaging	1.00
R8881:Akap9	UTSW	5	4,011,279 (GRCm39)	missense
R8937:Akap9	UTSW	5	4,094,048 (GRCm39)	missense	possibly damaging	0.78
R8956:Akap9	UTSW	5	3,998,805 (GRCm39)	missense	possibly damaging	0.79
R9000:Akap9	UTSW	5	4,105,650 (GRCm39)	missense	probably benign
R9049:Akap9	UTSW	5	4,114,597 (GRCm39)	missense
R9074:Akap9	UTSW	5	4,127,959 (GRCm39)	missense	probably benign	0.40
R9124:Akap9	UTSW	5	4,111,284 (GRCm39)	missense	probably damaging	0.99
R9129:Akap9	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
R9371:Akap9	UTSW	5	4,011,852 (GRCm39)	missense	possibly damaging	0.83
R9424:Akap9	UTSW	5	4,012,224 (GRCm39)	nonsense	probably null
R9424:Akap9	UTSW	5	4,012,223 (GRCm39)	nonsense	probably null
R9509:Akap9	UTSW	5	4,096,349 (GRCm39)	missense	probably benign
R9515:Akap9	UTSW	5	4,105,709 (GRCm39)	missense	probably damaging	1.00
R9567:Akap9	UTSW	5	4,127,311 (GRCm39)	missense	possibly damaging	0.89
R9587:Akap9	UTSW	5	4,119,149 (GRCm39)	missense	probably damaging	1.00
R9619:Akap9	UTSW	5	4,094,833 (GRCm39)	missense	probably damaging	1.00
R9635:Akap9	UTSW	5	4,100,545 (GRCm39)	missense	probably benign	0.20
R9680:Akap9	UTSW	5	4,011,587 (GRCm39)	missense	probably benign	0.03
R9691:Akap9	UTSW	5	4,010,491 (GRCm39)	missense	probably damaging	1.00
R9726:Akap9	UTSW	5	4,053,757 (GRCm39)	missense	probably benign	0.39
U15987:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
X0026:Akap9	UTSW	5	4,064,039 (GRCm39)	missense	probably damaging	1.00
X0057:Akap9	UTSW	5	4,025,598 (GRCm39)	critical splice acceptor site	probably null
Z1176:Akap9	UTSW	5	4,012,251 (GRCm39)	missense	probably damaging	0.96
Z1177:Akap9	UTSW	5	4,096,189 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAGGCAGACAATCGCAGAGC -3'
(R):5'- TCAGGTTGTCTGTTTCACACTG -3'

Sequencing Primer
(F):5'- CAATCGCAGAGCAAGAGAAC -3'
(R):5'- AGGTTGTCTGTTTCACACTGCATAC -3'

Posted On

2015-05-15