Mutagenetix > Incidental Mutation

Incidental Mutation 'R4082:Or13n4'

316937

Institutional Source

Beutler Lab

Gene Symbol

Or13n4

Ensembl Gene

ENSMUSG00000056863

Gene Name

olfactory receptor family 13 subfamily N member 4

Synonyms

GA_x6K02T2PBJ9-9202245-9201289, MOR260-4, Olfr702

MMRRC Submission

041624-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R4082 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

106422696-106425950 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 106423245 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 163 (T163A)

Ref Sequence

ENSEMBL: ENSMUSP00000151862 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075414] [ENSMUST00000080899] [ENSMUST00000166880] [ENSMUST00000208864] [ENSMUST00000208895] [ENSMUST00000217739] [ENSMUST00000219803]

AlphaFold

Q920Z2

Predicted Effect

probably benign
Transcript: ENSMUST00000075414

SMART Domains

Protein: ENSMUSP00000074868
Gene: ENSMUSG00000036744

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.9e-46	PFAM
Pfam:7tm_1	41	290	2.3e-24	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000080899
AA Change: T163A

PolyPhen 2 Score 0.780 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000079704
Gene: ENSMUSG00000056863
AA Change: T163A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	307	2.7e-53	PFAM
Pfam:7TM_GPCR_Srsx	35	300	2.9e-8	PFAM
Pfam:7tm_1	41	290	1.5e-26	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000166880

SMART Domains

Protein: ENSMUSP00000130958
Gene: ENSMUSG00000036744

Domain	Start	End	E-Value	Type
Pfam:7tm_1	41	290	5.8e-31	PFAM
Pfam:7tm_4	139	283	1.8e-44	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000208864

Predicted Effect

probably benign
Transcript: ENSMUST00000208895

Predicted Effect

probably benign
Transcript: ENSMUST00000217739

Predicted Effect

possibly damaging
Transcript: ENSMUST00000219803
AA Change: T163A

PolyPhen 2 Score 0.780 (Sensitivity: 0.85; Specificity: 0.93)

Meta Mutation Damage Score

0.4034

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

99% (73/74)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700021P04Rik	T	G	19: 24,043,366 (GRCm39)		noncoding transcript	Het
a	A	T	2: 154,887,678 (GRCm39)	D46V	probably damaging	Het
Aass	A	T	6: 23,109,497 (GRCm39)	D324E	possibly damaging	Het
Abca12	G	T	1: 71,306,622 (GRCm39)	T2028K	possibly damaging	Het
Abt1	T	C	13: 23,606,316 (GRCm39)	T213A	probably benign	Het
Adcy1	A	C	11: 7,014,117 (GRCm39)	Y173S	probably damaging	Het
Aim2	T	C	1: 173,287,417 (GRCm39)		probably null	Het
Akr1d1	G	A	6: 37,534,424 (GRCm39)	V193M	probably damaging	Het
Cars1	C	T	7: 143,123,234 (GRCm39)	E461K	probably damaging	Het
Ccdc80	T	C	16: 44,943,290 (GRCm39)	L800P	probably damaging	Het
Ccl22	A	G	8: 95,473,536 (GRCm39)	Y27C	probably damaging	Het
Cdc123	G	A	2: 5,815,566 (GRCm39)		probably benign	Het
Cldn11	A	T	3: 31,217,278 (GRCm39)	I149F	probably benign	Het
Col14a1	T	C	15: 55,300,429 (GRCm39)	Y986H	unknown	Het
Col6a3	G	A	1: 90,749,605 (GRCm39)	L410F	probably damaging	Het
Crocc	T	C	4: 140,761,282 (GRCm39)		probably null	Het
Cubn	A	G	2: 13,433,374 (GRCm39)		probably benign	Het
Cwc25	G	T	11: 97,644,744 (GRCm39)	Q205K	probably benign	Het
Cyp2e1	T	C	7: 140,350,991 (GRCm39)	I321T	possibly damaging	Het
Eps8l1	T	A	7: 4,473,797 (GRCm39)		probably null	Het
Fasl	C	T	1: 161,609,420 (GRCm39)	V189M	probably damaging	Het
Fbxw5	T	C	2: 25,394,643 (GRCm39)		probably null	Het
Flg2	A	C	3: 93,110,828 (GRCm39)	E952A	unknown	Het
Gpd1l	A	T	9: 114,746,146 (GRCm39)	L90Q	probably damaging	Het
Grik4	G	T	9: 42,509,180 (GRCm39)	F414L	probably benign	Het
Kcnh8	GAGACCAACGAGCAGCTGATGCTTCAGA	GAGA	17: 53,032,934 (GRCm39)	74	probably benign	Het
Klhl3	C	T	13: 58,166,611 (GRCm39)	G407S	probably null	Het
Lmbr1	T	C	5: 29,463,753 (GRCm39)	E157G	probably damaging	Het
Lrp2	T	A	2: 69,343,617 (GRCm39)	H914L	probably damaging	Het
Mrpl20	A	T	4: 155,892,970 (GRCm39)	D67V	probably damaging	Het
Myo15a	A	G	11: 60,378,022 (GRCm39)	T1346A	possibly damaging	Het
Naip5	A	T	13: 100,382,338 (GRCm39)	C124S	probably damaging	Het
Or10ag2	T	A	2: 87,248,801 (GRCm39)	Y134*	probably null	Het
Or52u1	T	A	7: 104,237,830 (GRCm39)	V290D	probably damaging	Het
Osbp	A	G	19: 11,956,030 (GRCm39)	D385G	probably benign	Het
Paip1	G	A	13: 119,593,540 (GRCm39)	D460N	probably damaging	Het
Pde3b	T	C	7: 114,093,823 (GRCm39)	S356P	probably benign	Het
Pms2	A	G	5: 143,867,837 (GRCm39)	M814V	probably damaging	Het
Polg	C	A	7: 79,114,576 (GRCm39)	K128N	probably damaging	Het
Polk	G	T	13: 96,620,181 (GRCm39)	T694K	probably benign	Het
Pom121	T	C	5: 135,417,491 (GRCm39)	K342R	unknown	Het
Pou5f2	T	C	13: 78,174,024 (GRCm39)	L322P	probably damaging	Het
Prorp	G	T	12: 55,351,398 (GRCm39)	V236F	possibly damaging	Het
Ptpn6	T	C	6: 124,705,382 (GRCm39)	D183G	probably damaging	Het
Pygb	G	A	2: 150,668,391 (GRCm39)		probably null	Het
Ralgds	C	T	2: 28,442,283 (GRCm39)		probably benign	Het
Ret	T	C	6: 118,130,927 (GRCm39)	T1079A	possibly damaging	Het
Rspo2	A	C	15: 42,885,933 (GRCm39)	V241G	probably benign	Het
Smg1	T	A	7: 117,759,469 (GRCm39)		probably benign	Het
Snph	T	C	2: 151,435,722 (GRCm39)	D402G	probably damaging	Het
Spta1	A	G	1: 174,041,632 (GRCm39)	D1334G	probably benign	Het
Stard13	C	A	5: 151,016,294 (GRCm39)		probably null	Het
Sufu	A	G	19: 46,413,541 (GRCm39)	M141V	probably damaging	Het
Sytl2	T	A	7: 90,057,635 (GRCm39)	V831D	possibly damaging	Het
Tc2n	T	C	12: 101,617,414 (GRCm39)	E335G	possibly damaging	Het
Tex11	C	A	X: 99,977,021 (GRCm39)	A487S	possibly damaging	Het
Tmcc1	T	A	6: 116,020,441 (GRCm39)	H118L	probably damaging	Het
Tulp4	C	T	17: 6,282,055 (GRCm39)	H695Y	probably damaging	Het
Vmn1r209	A	C	13: 22,989,785 (GRCm39)	L302V	probably null	Het
Vmn2r117	C	T	17: 23,679,080 (GRCm39)	V715I	probably benign	Het
Vopp1	A	T	6: 57,766,964 (GRCm39)	Y37*	probably null	Het
Xrn1	A	G	9: 95,863,973 (GRCm39)	T528A	probably benign	Het
Zfhx2	T	C	14: 55,302,662 (GRCm39)	D1774G	probably benign	Het
Zfp955b	T	A	17: 33,521,129 (GRCm39)	D199E	probably benign	Het
Zp2	T	C	7: 119,734,475 (GRCm39)	S525G	probably benign	Het

Other mutations in Or13n4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01549:Or13n4	APN	7	106,423,236 (GRCm39)	missense	probably benign	0.01
IGL02120:Or13n4	APN	7	106,422,905 (GRCm39)	missense	possibly damaging	0.94
R0025:Or13n4	UTSW	7	106,422,963 (GRCm39)	missense	possibly damaging	0.74
R1213:Or13n4	UTSW	7	106,423,404 (GRCm39)	missense	possibly damaging	0.48
R1830:Or13n4	UTSW	7	106,423,317 (GRCm39)	missense	probably benign	0.00
R2216:Or13n4	UTSW	7	106,423,205 (GRCm39)	missense	probably damaging	0.99
R2571:Or13n4	UTSW	7	106,422,933 (GRCm39)	missense	probably benign	0.09
R2876:Or13n4	UTSW	7	106,423,664 (GRCm39)	missense	probably benign	0.07
R2920:Or13n4	UTSW	7	106,423,571 (GRCm39)	missense	probably benign	0.11
R4130:Or13n4	UTSW	7	106,422,792 (GRCm39)	missense	probably benign	0.01
R4750:Or13n4	UTSW	7	106,423,514 (GRCm39)	missense	probably damaging	0.98
R5007:Or13n4	UTSW	7	106,423,364 (GRCm39)	missense	probably damaging	1.00
R5117:Or13n4	UTSW	7	106,422,869 (GRCm39)	missense	probably damaging	0.99
R5908:Or13n4	UTSW	7	106,423,404 (GRCm39)	missense	probably benign	0.09
R6824:Or13n4	UTSW	7	106,423,664 (GRCm39)	missense	probably benign	0.00
R7193:Or13n4	UTSW	7	106,423,798 (GRCm39)	start gained	probably benign
R7254:Or13n4	UTSW	7	106,422,777 (GRCm39)	makesense	probably null
R7827:Or13n4	UTSW	7	106,422,932 (GRCm39)	missense	probably benign	0.01
R8309:Or13n4	UTSW	7	106,423,620 (GRCm39)	missense	probably benign	0.00
R8410:Or13n4	UTSW	7	106,423,745 (GRCm39)	start gained	probably benign
R9353:Or13n4	UTSW	7	106,423,062 (GRCm39)	missense	probably benign	0.00
R9386:Or13n4	UTSW	7	106,423,707 (GRCm39)	missense	probably benign
R9562:Or13n4	UTSW	7	106,423,020 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGACCTTGAGCCTTCCCTC -3'
(R):5'- TCTTTCTCCTAGCAGGGTGC -3'

Sequencing Primer
(F):5'- ATCACAGTGGAGATGATGTTCCC -3'
(R):5'- GCAGGGTGCACAGAGTG -3'

Posted On

2015-05-15