Mutagenetix > Incidental Mutation

Incidental Mutation 'R4084:Ifi44'

317012

Institutional Source

Beutler Lab

Gene Symbol

Ifi44

Ensembl Gene

ENSMUSG00000028037

Gene Name

interferon-induced protein 44

Synonyms

A430056A10Rik, p44, MTAP44

MMRRC Submission

040857-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4084 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

151436559-151455580 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 151451126 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000029671 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029671]

AlphaFold

Q8BV66

Predicted Effect

probably null
Transcript: ENSMUST00000029671

SMART Domains

Protein: ENSMUSP00000029671
Gene: ENSMUSG00000028037

Domain	Start	End	E-Value	Type
Pfam:TLD	26	147	2.8e-7	PFAM
low complexity region	193	208	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127733

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133888

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149919

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Amotl2	A	T	9: 102,601,884 (GRCm39)		probably null	Het
Arhgdig	T	C	17: 26,418,799 (GRCm39)	D114G	possibly damaging	Het
Btnl1	C	T	17: 34,600,133 (GRCm39)	T212I	possibly damaging	Het
Camkk1	C	T	11: 72,928,691 (GRCm39)	T410I	probably damaging	Het
Capn13	C	T	17: 73,644,444 (GRCm39)	G362R	probably benign	Het
Catsperd	A	G	17: 56,961,453 (GRCm39)	T392A	probably benign	Het
Ccdc180	A	G	4: 45,950,632 (GRCm39)	I1626V	probably benign	Het
Cdon	C	A	9: 35,389,427 (GRCm39)	T844K	probably damaging	Het
Col28a1	G	A	6: 8,013,131 (GRCm39)	Q974*	probably null	Het
Col28a1	C	G	6: 8,013,132 (GRCm39)	K973N	possibly damaging	Het
Dnhd1	T	C	7: 105,358,795 (GRCm39)	L3428P	probably damaging	Het
Ecm1	A	T	3: 95,641,676 (GRCm39)	N519K	probably damaging	Het
Fbxw11	T	C	11: 32,689,248 (GRCm39)	V457A	probably damaging	Het
Flna	C	T	X: 73,280,531 (GRCm39)	V1009M	possibly damaging	Het
Fmnl2	A	T	2: 52,997,507 (GRCm39)	K486I	possibly damaging	Het
Gja1	A	C	10: 56,264,607 (GRCm39)	Q322P	possibly damaging	Het
Gtpbp3	A	G	8: 71,943,156 (GRCm39)	Q189R	probably benign	Het
H2-Eb1	T	C	17: 34,533,417 (GRCm39)	V213A	probably damaging	Het
Herc4	G	T	10: 63,119,016 (GRCm39)	G322V	probably damaging	Het
Hgf	G	T	5: 16,820,856 (GRCm39)	G668*	probably null	Het
Htra1	T	C	7: 130,538,074 (GRCm39)	S25P	probably benign	Het
Klhl24	T	A	16: 19,933,312 (GRCm39)	S308T	probably damaging	Het
Lamb2	A	G	9: 108,365,217 (GRCm39)	N1291S	probably benign	Het
Lgals9	A	T	11: 78,860,589 (GRCm39)	F162Y	possibly damaging	Het
Lig3	T	A	11: 82,686,250 (GRCm39)	I634N	probably damaging	Het
Lipn	T	C	19: 34,056,340 (GRCm39)	F229L	probably benign	Het
Lmtk3	T	A	7: 45,442,716 (GRCm39)	S466R	probably damaging	Het
Lonrf2	G	A	1: 38,860,232 (GRCm39)	T22I	probably benign	Het
Macf1	T	C	4: 123,343,865 (GRCm39)	H2119R	probably damaging	Het
Muc6	C	T	7: 141,234,920 (GRCm39)	C634Y	probably damaging	Het
Nap1l1	G	A	10: 111,325,938 (GRCm39)	V86I	possibly damaging	Het
Noxred1	A	G	12: 87,280,258 (GRCm39)	Y25H	possibly damaging	Het
Nphp4	T	C	4: 152,573,248 (GRCm39)	L62P	probably damaging	Het
Or11h6	T	A	14: 50,880,305 (GRCm39)	I189N	probably damaging	Het
Or2b2	G	A	13: 21,887,238 (GRCm39)	W22*	probably null	Het
Or2b2	C	A	13: 21,887,239 (GRCm39)	L23M	probably damaging	Het
Or52ab4	T	G	7: 102,987,527 (GRCm39)	F89V	probably damaging	Het
Pcdh10	A	C	3: 45,347,142 (GRCm39)	D979A	probably damaging	Het
Pla2g4f	C	G	2: 120,142,806 (GRCm39)	Q101H	probably benign	Het
Ppp1r15b	T	C	1: 133,060,805 (GRCm39)	F441L	probably damaging	Het
Prkaca	C	A	8: 84,721,939 (GRCm39)	P309T	probably damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Ripor3	A	G	2: 167,826,386 (GRCm39)	Y720H	possibly damaging	Het
Rpgrip1	A	G	14: 52,386,808 (GRCm39)	E751G	possibly damaging	Het
Rsf1	GCGGCGGCGGCGGCGGC	GCGGCGGCGGCGGCGGCGGCGGCGGC	7: 97,229,126 (GRCm39)		probably benign	Het
Ryr3	A	G	2: 112,731,253 (GRCm39)	S686P	probably damaging	Het
Seh1l	T	C	18: 67,921,860 (GRCm39)	V240A	possibly damaging	Het
Slc10a2	T	G	8: 5,139,126 (GRCm39)	I273L	possibly damaging	Het
Slc23a2	A	T	2: 131,933,137 (GRCm39)	L107*	probably null	Het
Slc44a4	T	C	17: 35,136,323 (GRCm39)	L38P	probably damaging	Het
Slc6a18	C	T	13: 73,815,148 (GRCm39)	V387I	probably benign	Het
Slu7	G	A	11: 43,334,218 (GRCm39)	A415T	probably benign	Het
Tlr5	A	T	1: 182,802,413 (GRCm39)	R572S	possibly damaging	Het
Tmem45a2	C	T	16: 56,891,387 (GRCm39)	G3D	probably benign	Het
Trim24	A	G	6: 37,892,192 (GRCm39)	T242A	probably damaging	Het
Triobp	T	A	15: 78,857,871 (GRCm39)	N1157K	probably benign	Het
Ugt1a6a	A	T	1: 88,066,899 (GRCm39)	D235V	probably benign	Het
Vmn2r37	C	T	7: 9,218,984 (GRCm39)	V467I	probably benign	Het
Vmn2r7	C	T	3: 64,600,414 (GRCm39)	E495K	probably benign	Het
Vstm2a	A	G	11: 16,213,098 (GRCm39)	E161G	probably damaging	Het
Ypel3	T	C	7: 126,377,537 (GRCm39)	V74A	possibly damaging	Het
Zfp1005	T	A	2: 150,108,122 (GRCm39)	N27K	possibly damaging	Het
Zfp27	C	T	7: 29,594,792 (GRCm39)	R391H	possibly damaging	Het

Other mutations in Ifi44

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00858:Ifi44	APN	3	151,455,217 (GRCm39)	missense	probably benign	0.03
IGL01477:Ifi44	APN	3	151,451,635 (GRCm39)	splice site	probably benign
IGL01571:Ifi44	APN	3	151,451,174 (GRCm39)	missense	probably damaging	0.97
IGL02165:Ifi44	APN	3	151,455,067 (GRCm39)	missense	probably damaging	1.00
IGL02238:Ifi44	APN	3	151,438,019 (GRCm39)	makesense	probably null
IGL02609:Ifi44	APN	3	151,438,134 (GRCm39)	missense	probably damaging	1.00
IGL02650:Ifi44	APN	3	151,451,492 (GRCm39)	missense	probably damaging	1.00
IGL02726:Ifi44	APN	3	151,455,233 (GRCm39)	start gained	probably benign
IGL02977:Ifi44	APN	3	151,445,016 (GRCm39)	missense	probably benign	0.00
R0201:Ifi44	UTSW	3	151,451,273 (GRCm39)	missense	probably damaging	1.00
R0454:Ifi44	UTSW	3	151,451,134 (GRCm39)	missense	possibly damaging	0.78
R0763:Ifi44	UTSW	3	151,455,135 (GRCm39)	missense	probably damaging	1.00
R1640:Ifi44	UTSW	3	151,438,171 (GRCm39)	missense	probably benign	0.00
R1747:Ifi44	UTSW	3	151,454,922 (GRCm39)	missense	probably benign	0.00
R2278:Ifi44	UTSW	3	151,438,025 (GRCm39)	missense	probably benign
R3816:Ifi44	UTSW	3	151,454,894 (GRCm39)	missense	possibly damaging	0.92
R4075:Ifi44	UTSW	3	151,451,613 (GRCm39)	missense	probably benign	0.00
R4782:Ifi44	UTSW	3	151,451,229 (GRCm39)	missense	probably damaging	0.99
R5071:Ifi44	UTSW	3	151,455,269 (GRCm39)	start gained	probably benign
R5074:Ifi44	UTSW	3	151,455,269 (GRCm39)	start gained	probably benign
R6030:Ifi44	UTSW	3	151,455,195 (GRCm39)	missense	probably benign	0.00
R6030:Ifi44	UTSW	3	151,455,195 (GRCm39)	missense	probably benign	0.00
R6128:Ifi44	UTSW	3	151,454,823 (GRCm39)	missense	probably benign	0.00
R6192:Ifi44	UTSW	3	151,451,276 (GRCm39)	critical splice acceptor site	probably null
R6434:Ifi44	UTSW	3	151,454,826 (GRCm39)	missense	probably benign	0.02
R6536:Ifi44	UTSW	3	151,438,126 (GRCm39)	missense	probably benign	0.03
R6902:Ifi44	UTSW	3	151,451,536 (GRCm39)	missense	possibly damaging	0.92
R6946:Ifi44	UTSW	3	151,451,536 (GRCm39)	missense	possibly damaging	0.92
R7346:Ifi44	UTSW	3	151,438,094 (GRCm39)	missense	probably benign
R7608:Ifi44	UTSW	3	151,438,045 (GRCm39)	missense	probably damaging	0.97
R7704:Ifi44	UTSW	3	151,438,061 (GRCm39)	missense	probably benign	0.44
R7971:Ifi44	UTSW	3	151,454,857 (GRCm39)	missense	possibly damaging	0.89
R8255:Ifi44	UTSW	3	151,451,619 (GRCm39)	missense	probably benign	0.00
R8428:Ifi44	UTSW	3	151,444,978 (GRCm39)	nonsense	probably null
R8940:Ifi44	UTSW	3	151,454,946 (GRCm39)	missense	probably benign	0.00
R9087:Ifi44	UTSW	3	151,451,517 (GRCm39)	missense	probably damaging	1.00
R9259:Ifi44	UTSW	3	151,454,875 (GRCm39)	missense	possibly damaging	0.75
R9436:Ifi44	UTSW	3	151,454,886 (GRCm39)	missense	probably benign	0.00
R9516:Ifi44	UTSW	3	151,438,108 (GRCm39)	missense	probably damaging	0.97
R9619:Ifi44	UTSW	3	151,451,509 (GRCm39)	missense	probably damaging	0.97
Z1176:Ifi44	UTSW	3	151,438,090 (GRCm39)	missense	probably damaging	1.00
Z1177:Ifi44	UTSW	3	151,455,075 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCCCTTATAATAGGTGTAACAGAG -3'
(R):5'- TGGCCAGTCAATCTTATGTAGAC -3'

Sequencing Primer
(F):5'- GTGCAGTTTAGCATGCTC -3'
(R):5'- CATCAAGGCCAAGGATGA -3'

Posted On

2015-05-15