Incidental Mutation 'R4224:Mettl21a'
ID319946
Institutional Source Beutler Lab
Gene Symbol Mettl21a
Ensembl Gene ENSMUSG00000025956
Gene Namemethyltransferase like 21A
Synonyms2310038H17Rik, Fam119a
MMRRC Submission 041044-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.055) question?
Stock #R4224 (G1)
Quality Score225
Status Validated
Chromosome1
Chromosomal Location64606473-64617242 bp(-) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) G to T at 64607956 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Stop codon at position 147 (Y147*)
Ref Sequence ENSEMBL: ENSMUSP00000109713 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053469] [ENSMUST00000087366] [ENSMUST00000114079]
Predicted Effect probably null
Transcript: ENSMUST00000053469
AA Change: Y147*
SMART Domains Protein: ENSMUSP00000050424
Gene: ENSMUSG00000025956
AA Change: Y147*

DomainStartEndE-ValueType
Pfam:Methyltransf_16 25 190 1.8e-53 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000087366
SMART Domains Protein: ENSMUSP00000084624
Gene: ENSMUSG00000025958

DomainStartEndE-ValueType
internal_repeat_1 16 90 1.46e-5 PROSPERO
Pfam:pKID 99 141 5.3e-24 PFAM
low complexity region 148 160 N/A INTRINSIC
internal_repeat_1 170 247 1.46e-5 PROSPERO
BRLZ 267 325 1.25e-6 SMART
Predicted Effect probably null
Transcript: ENSMUST00000114079
AA Change: Y147*
SMART Domains Protein: ENSMUSP00000109713
Gene: ENSMUSG00000025956
AA Change: Y147*

DomainStartEndE-ValueType
Pfam:Methyltransf_16 25 190 1e-53 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000162806
Meta Mutation Damage Score 0.6252 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 96.0%
Validation Efficiency 95% (42/44)
Allele List at MGI
Other mutations in this stock
Total: 38 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9330182O14Rik T C 15: 40,142,276 probably null Het
Abl2 A G 1: 156,633,847 T440A probably damaging Het
Acat2 T C 17: 12,962,885 probably benign Het
Acsbg1 C T 9: 54,616,041 R434H possibly damaging Het
Aif1l A T 2: 31,962,239 S40C probably damaging Het
Atp6v1a C A 16: 44,101,811 A355S probably damaging Het
Corin T C 5: 72,343,108 E391G probably damaging Het
Dnah11 T G 12: 118,130,892 S814R probably benign Het
Fam214a T A 9: 75,008,726 N209K probably damaging Het
Fer1l4 A C 2: 156,020,389 V1788G probably benign Het
Gm7964 T G 7: 83,756,822 N281K probably damaging Het
Grin1 T C 2: 25,297,320 probably benign Het
Ica1 T C 6: 8,659,960 K112R probably benign Het
Itpr3 T C 17: 27,107,258 V1334A probably damaging Het
Khdc3 C G 9: 73,102,871 H70D possibly damaging Het
Lama3 T C 18: 12,450,403 C683R probably damaging Het
Mpp5 A G 12: 78,829,718 K479E probably damaging Het
Mr1 A T 1: 155,130,719 I294N possibly damaging Het
Olfr617 A G 7: 103,584,759 T246A probably damaging Het
Olfr862 T A 9: 19,883,600 Y235F probably benign Het
Pcdhgb7 T A 18: 37,753,803 D675E probably benign Het
Ptbp1 T C 10: 79,859,213 I125T probably benign Het
Rnf213 C T 11: 119,436,823 R1879* probably null Het
Slc8a1 A G 17: 81,649,352 F86L probably damaging Het
Spdya G A 17: 71,562,524 V105I probably benign Het
Sspo G T 6: 48,451,157 V313L possibly damaging Het
Stard9 A G 2: 120,664,991 T116A possibly damaging Het
Tnnt1 T C 7: 4,510,007 H92R probably damaging Het
Trim37 T A 11: 87,216,463 C907S probably damaging Het
Trim43b T A 9: 89,090,639 Q154L probably benign Het
Tssk3 T C 4: 129,490,599 D3G probably benign Het
Ubash3a T C 17: 31,237,928 Y521H probably damaging Het
Vmn1r73 T C 7: 11,756,579 I108T probably damaging Het
Vmn2r14 A G 5: 109,216,283 M589T probably benign Het
Vmn2r60 A T 7: 42,116,528 T20S probably benign Het
Vps13b A G 15: 35,876,419 T2799A probably damaging Het
Zcchc18 A T X: 136,994,666 N10I probably damaging Het
Znrd1as T C 17: 36,958,725 probably benign Het
Other mutations in Mettl21a
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02500:Mettl21a APN 1 64608054 missense probably benign 0.13
R2219:Mettl21a UTSW 1 64616283 missense probably damaging 1.00
R2220:Mettl21a UTSW 1 64616283 missense probably damaging 1.00
R3902:Mettl21a UTSW 1 64608081 missense probably benign 0.03
R5715:Mettl21a UTSW 1 64615155 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- AAATGTGTACGTCCTTCTCAGG -3'
(R):5'- GGTCGTACGATGTTCACCTTC -3'

Sequencing Primer
(F):5'- GGATCGTAGTGAACCTTACTCACAG -3'
(R):5'- CCACATTGTAGGTGCTCAGG -3'
Posted On2015-06-12