Mutagenetix > Incidental Mutation

Incidental Mutation 'R4229:Gm10735'

320138

Institutional Source

Beutler Lab

Gene Symbol

Gm10735

Ensembl Gene

ENSMUSG00000074650

Gene Name

predicted gene 10735

Synonyms

MMRRC Submission

041048-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R4229 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

113175835-113178777 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to C at 113177743 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000156385 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022282] [ENSMUST00000099162] [ENSMUST00000109244] [ENSMUST00000180543] [ENSMUST00000181117] [ENSMUST00000181568] [ENSMUST00000231962]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000022282

SMART Domains

Protein: ENSMUSP00000022282
Gene: ENSMUSG00000021760

Domain	Start	End	E-Value	Type
Pfam:AhpC-TSA	44	143	1.3e-8	PFAM
Pfam:GSHPx	47	155	5.3e-36	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000083641

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000099162

SMART Domains

Protein: ENSMUSP00000096766
Gene: ENSMUSG00000074650

Domain	Start	End	E-Value	Type
low complexity region	3	12	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000104833

Predicted Effect

probably benign
Transcript: ENSMUST00000109244

SMART Domains

Protein: ENSMUSP00000104867
Gene: ENSMUSG00000078926

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	391	432	3.55e1	SMART
WD40	473	517	2.01e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000180543

Predicted Effect

probably benign
Transcript: ENSMUST00000181117

SMART Domains

Protein: ENSMUSP00000137915
Gene: ENSMUSG00000078926

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	391	432	3.55e1	SMART
WD40	473	513	1.78e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181568

SMART Domains

Protein: ENSMUSP00000137849
Gene: ENSMUSG00000078926

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	431	475	2.01e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000231962

Predicted Effect

probably benign
Transcript: ENSMUST00000181741

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.0%
20x: 94.5%

Validation Efficiency

98% (42/43)

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	C	T	6: 128,557,349 (GRCm39)	A115T	probably benign	Het
Aadac	A	G	3: 59,939,234 (GRCm39)	K3E	possibly damaging	Het
Cdc14a	A	G	3: 116,087,413 (GRCm39)	S533P	probably damaging	Het
Dsc3	G	A	18: 20,098,878 (GRCm39)	T767I	probably damaging	Het
Dse	A	T	10: 34,038,740 (GRCm39)	M221K	probably damaging	Het
Etfb	C	A	7: 43,105,984 (GRCm39)	R174S	probably damaging	Het
Gm27013	A	T	6: 130,654,308 (GRCm39)	S385T	possibly damaging	Het
Gsap	A	G	5: 21,451,975 (GRCm39)	D339G	probably benign	Het
H2-T24	G	A	17: 36,325,721 (GRCm39)	A290V	probably benign	Het
Ifi44l	A	T	3: 151,468,514 (GRCm39)	C5*	probably null	Het
Lipk	A	G	19: 33,997,687 (GRCm39)	Y56C	probably damaging	Het
Mrpl21	A	G	19: 3,336,901 (GRCm39)	I91V	probably damaging	Het
Nin	T	C	12: 70,097,984 (GRCm39)	E492G	probably damaging	Het
Nxpe4	A	G	9: 48,304,122 (GRCm39)	R70G	possibly damaging	Het
Or1e1	A	T	11: 73,245,058 (GRCm39)	T160S	probably damaging	Het
Or4k36	A	G	2: 111,146,681 (GRCm39)	I286V	probably damaging	Het
Or4p18	T	A	2: 88,233,227 (GRCm39)	Q17L	possibly damaging	Het
Or52e18	A	T	7: 104,609,801 (GRCm39)	I46N	probably benign	Het
Plaur	T	C	7: 24,166,208 (GRCm39)	V93A	probably damaging	Het
Ptprv	C	A	1: 135,053,945 (GRCm39)		noncoding transcript	Het
Rabep1	A	G	11: 70,799,260 (GRCm39)	T295A	probably benign	Het
Sec24b	G	T	3: 129,834,368 (GRCm39)	Q141K	probably benign	Het
Set	A	G	2: 29,959,531 (GRCm39)	Y139C	probably damaging	Het
Sspo	T	C	6: 48,467,868 (GRCm39)	S4272P	probably benign	Het
Tmc3	T	C	7: 83,246,610 (GRCm39)		probably benign	Het
Tmem229a	T	A	6: 24,954,831 (GRCm39)	I308F	probably damaging	Het
Vmn1r223	A	T	13: 23,433,585 (GRCm39)	M60L	probably benign	Het
Zfp148	A	G	16: 33,255,133 (GRCm39)	E41G	probably benign	Het
Zfp677	T	C	17: 21,618,544 (GRCm39)	S534P	probably damaging	Het

Other mutations in Gm10735

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL00157:Gm10735	APN	13	113,178,018 (GRCm39)	unclassified	probably benign
R4230:Gm10735	UTSW	13	113,177,743 (GRCm39)	unclassified	probably benign
R6220:Gm10735	UTSW	13	113,178,030 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- TCAGCGAAGGTACATGCAAC -3'
(R):5'- ATTCTGATGTCCTCAAGTCACC -3'

Sequencing Primer
(F):5'- GGTACATGCAACCACATCTCTG -3'
(R):5'- GGGGCCATATCAAGAGCTTC -3'

Posted On

2015-06-12