Mutagenetix > Incidental Mutation

Incidental Mutation 'R4294:Vmn2r74'

323219

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r74

Ensembl Gene

ENSMUSG00000090774

Gene Name

vomeronasal 2, receptor 74

Synonyms

EG546980

MMRRC Submission

041083-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R4294 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85601075-85610690 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 85606624 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 241 (I241F)

Ref Sequence

ENSEMBL: ENSMUSP00000126917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166355]

AlphaFold

E9PW21

Predicted Effect

probably benign
Transcript: ENSMUST00000166355
AA Change: I241F

PolyPhen 2 Score 0.137 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000126917
Gene: ENSMUSG00000090774
AA Change: I241F

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	467	7.3e-28	PFAM
Pfam:NCD3G	510	562	4.7e-20	PFAM
Pfam:7tm_3	592	830	1.3e-52	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059E24Rik	A	T	19: 21,576,113 (GRCm39)		probably null	Het
Abca3	A	G	17: 24,619,543 (GRCm39)	I960M	possibly damaging	Het
Bivm	A	T	1: 44,177,793 (GRCm39)	R364S	probably damaging	Het
Bsnd	C	T	4: 106,342,355 (GRCm39)	R271H	probably benign	Het
Cckar	A	G	5: 53,863,839 (GRCm39)	S41P	probably benign	Het
Clip2	A	C	5: 134,521,167 (GRCm39)	V957G	probably benign	Het
Cyp2c55	A	T	19: 39,000,235 (GRCm39)	I145F	probably damaging	Het
Cyp3a11	A	T	5: 145,806,005 (GRCm39)	S121T	probably benign	Het
Dlc1	A	G	8: 37,051,907 (GRCm39)	V608A	possibly damaging	Het
Dlg3	A	T	X: 99,840,288 (GRCm39)		probably benign	Het
Dock3	T	A	9: 106,807,242 (GRCm39)	R1362W	probably damaging	Het
Fhdc1	C	A	3: 84,352,133 (GRCm39)	V1031F	probably benign	Het
Gimap8	A	T	6: 48,635,891 (GRCm39)	H552L	probably benign	Het
Gpr151	T	C	18: 42,711,602 (GRCm39)	T359A	probably benign	Het
Gucy1a1	A	T	3: 82,002,066 (GRCm39)	F671Y	possibly damaging	Het
Kcnv1	G	A	15: 44,977,840 (GRCm39)	T66M	probably damaging	Het
Kif18a	T	C	2: 109,123,398 (GRCm39)	V224A	probably benign	Het
Lbr	C	T	1: 181,648,267 (GRCm39)	C398Y	probably damaging	Het
Magel2	T	G	7: 62,028,515 (GRCm39)	V473G	possibly damaging	Het
Mapkapk3	T	C	9: 107,136,131 (GRCm39)		probably benign	Het
Nat8f1	T	C	6: 85,887,637 (GRCm39)	T108A	probably benign	Het
Nphp3	T	A	9: 103,899,916 (GRCm39)	L502Q	probably damaging	Het
Or2v2	T	A	11: 49,004,254 (GRCm39)	I100L	probably benign	Het
Otud7a	A	G	7: 63,346,939 (GRCm39)	D171G	probably damaging	Het
Pcdhb5	T	A	18: 37,455,734 (GRCm39)	S705T	possibly damaging	Het
Phf14	C	T	6: 11,987,096 (GRCm39)	P559S	probably damaging	Het
Rpl27-ps3	T	A	18: 6,332,607 (GRCm39)		probably null	Het
Sec16a	A	G	2: 26,312,167 (GRCm39)	Y1998H	probably benign	Het
Setd5	AT	ATT	6: 113,088,281 (GRCm39)		probably benign	Het
Sgsm1	A	G	5: 113,433,270 (GRCm39)	Y182H	probably damaging	Het
Slc22a21	T	C	11: 53,860,329 (GRCm39)	D34G	probably damaging	Het
Spata13	T	A	14: 60,947,004 (GRCm39)	M684K	probably damaging	Het
Srsf6	T	C	2: 162,776,636 (GRCm39)		probably benign	Het
Thrb	C	T	14: 18,011,145 (GRCm38)	Q174*	probably null	Het
Ticam1	A	G	17: 56,578,339 (GRCm39)	I252T	probably benign	Het
Tmem237	A	G	1: 59,158,995 (GRCm39)		probably benign	Het
Trpv1	C	T	11: 73,131,290 (GRCm39)	A276V	probably damaging	Het
Vmn1r192	T	A	13: 22,371,465 (GRCm39)	I252F	probably damaging	Het

Other mutations in Vmn2r74

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00899:Vmn2r74	APN	7	85,606,338 (GRCm39)	missense	probably benign	0.03
IGL00904:Vmn2r74	APN	7	85,606,788 (GRCm39)	missense	probably benign	0.05
IGL01285:Vmn2r74	APN	7	85,606,692 (GRCm39)	missense	possibly damaging	0.54
IGL01300:Vmn2r74	APN	7	85,606,414 (GRCm39)	missense	probably benign	0.00
IGL01410:Vmn2r74	APN	7	85,610,500 (GRCm39)	missense	possibly damaging	0.83
IGL01827:Vmn2r74	APN	7	85,606,800 (GRCm39)	missense	probably benign	0.00
IGL02094:Vmn2r74	APN	7	85,610,669 (GRCm39)	missense	probably benign	0.01
IGL02252:Vmn2r74	APN	7	85,606,531 (GRCm39)	missense	probably benign	0.41
IGL02349:Vmn2r74	APN	7	85,601,724 (GRCm39)	missense	probably damaging	0.99
IGL02438:Vmn2r74	APN	7	85,601,824 (GRCm39)	missense	probably damaging	0.98
IGL02554:Vmn2r74	APN	7	85,606,581 (GRCm39)	missense	probably benign	0.00
IGL03036:Vmn2r74	APN	7	85,601,900 (GRCm39)	nonsense	probably null
IGL03370:Vmn2r74	APN	7	85,607,265 (GRCm39)	missense	probably benign
R0115:Vmn2r74	UTSW	7	85,606,564 (GRCm39)	missense	probably benign	0.00
R0333:Vmn2r74	UTSW	7	85,601,491 (GRCm39)	missense	probably benign	0.06
R0415:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R0571:Vmn2r74	UTSW	7	85,601,629 (GRCm39)	missense	probably damaging	1.00
R0626:Vmn2r74	UTSW	7	85,610,517 (GRCm39)	nonsense	probably null
R0659:Vmn2r74	UTSW	7	85,605,122 (GRCm39)	splice site	probably benign
R1202:Vmn2r74	UTSW	7	85,610,545 (GRCm39)	missense	possibly damaging	0.83
R1473:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R1908:Vmn2r74	UTSW	7	85,601,650 (GRCm39)	missense	probably benign
R2079:Vmn2r74	UTSW	7	85,606,383 (GRCm39)	missense	probably benign	0.00
R2368:Vmn2r74	UTSW	7	85,610,522 (GRCm39)	missense	probably benign	0.39
R3782:Vmn2r74	UTSW	7	85,605,322 (GRCm39)	missense	probably benign	0.01
R3824:Vmn2r74	UTSW	7	85,607,466 (GRCm39)	missense	probably damaging	1.00
R3977:Vmn2r74	UTSW	7	85,607,345 (GRCm39)	missense	probably benign	0.01
R4182:Vmn2r74	UTSW	7	85,606,395 (GRCm39)	missense	possibly damaging	0.87
R4289:Vmn2r74	UTSW	7	85,606,562 (GRCm39)	missense	probably benign
R4645:Vmn2r74	UTSW	7	85,606,317 (GRCm39)	missense	probably benign
R4646:Vmn2r74	UTSW	7	85,606,782 (GRCm39)	missense	probably benign	0.42
R4655:Vmn2r74	UTSW	7	85,610,555 (GRCm39)	missense	probably benign
R4901:Vmn2r74	UTSW	7	85,605,199 (GRCm39)	nonsense	probably null
R5532:Vmn2r74	UTSW	7	85,601,197 (GRCm39)	missense	probably benign	0.32
R5642:Vmn2r74	UTSW	7	85,606,588 (GRCm39)	missense	probably benign	0.00
R5913:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6170:Vmn2r74	UTSW	7	85,606,348 (GRCm39)	missense	probably benign	0.03
R6232:Vmn2r74	UTSW	7	85,607,498 (GRCm39)	missense	possibly damaging	0.82
R6238:Vmn2r74	UTSW	7	85,601,280 (GRCm39)	missense	probably damaging	1.00
R6255:Vmn2r74	UTSW	7	85,601,659 (GRCm39)	missense	possibly damaging	0.90
R6468:Vmn2r74	UTSW	7	85,610,599 (GRCm39)	missense	probably benign	0.34
R6732:Vmn2r74	UTSW	7	85,606,758 (GRCm39)	missense	probably damaging	1.00
R6816:Vmn2r74	UTSW	7	85,610,621 (GRCm39)	nonsense	probably null
R6836:Vmn2r74	UTSW	7	85,606,630 (GRCm39)	missense	probably benign	0.00
R6995:Vmn2r74	UTSW	7	85,606,860 (GRCm39)	critical splice acceptor site	probably null
R6995:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R7186:Vmn2r74	UTSW	7	85,601,150 (GRCm39)	nonsense	probably null
R7246:Vmn2r74	UTSW	7	85,605,173 (GRCm39)	missense	probably benign
R7374:Vmn2r74	UTSW	7	85,606,630 (GRCm39)	missense	probably benign	0.02
R7505:Vmn2r74	UTSW	7	85,606,279 (GRCm39)	nonsense	probably null
R7525:Vmn2r74	UTSW	7	85,610,510 (GRCm39)	missense	probably benign
R7569:Vmn2r74	UTSW	7	85,601,544 (GRCm39)	missense	probably damaging	0.99
R7644:Vmn2r74	UTSW	7	85,606,746 (GRCm39)	missense	probably benign	0.11
R7956:Vmn2r74	UTSW	7	85,605,166 (GRCm39)	missense	probably benign	0.09
R8119:Vmn2r74	UTSW	7	85,610,690 (GRCm39)	start codon destroyed	probably null	0.08
R8131:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R8147:Vmn2r74	UTSW	7	85,605,227 (GRCm39)	nonsense	probably null
R8181:Vmn2r74	UTSW	7	85,605,324 (GRCm39)	missense	probably damaging	1.00
R8184:Vmn2r74	UTSW	7	85,601,454 (GRCm39)	missense	probably benign	0.00
R8375:Vmn2r74	UTSW	7	85,601,914 (GRCm39)	missense	possibly damaging	0.64
R8948:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R8950:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R9033:Vmn2r74	UTSW	7	85,606,414 (GRCm39)	missense	probably benign
R9342:Vmn2r74	UTSW	7	85,606,624 (GRCm39)	missense	probably benign	0.14
R9578:Vmn2r74	UTSW	7	85,606,101 (GRCm39)	missense	probably benign	0.01
R9607:Vmn2r74	UTSW	7	85,610,619 (GRCm39)	missense	probably benign	0.02
R9776:Vmn2r74	UTSW	7	85,605,212 (GRCm39)	missense	possibly damaging	0.70
Z1176:Vmn2r74	UTSW	7	85,604,835 (GRCm39)	missense	probably damaging	1.00
Z31818:Vmn2r74	UTSW	7	85,604,729 (GRCm39)	splice site	probably null

Predicted Primers

PCR Primer
(F):5'- GTGGACGCATATAGCAAGAAATCTC -3'
(R):5'- TTCACCAGATATCTCGCAAGG -3'

Sequencing Primer
(F):5'- GCAAGAAATCTCCTAGAAATGTGATC -3'
(R):5'- GATATCTCGCAAGGACACATTTC -3'

Posted On

2015-06-20