Incidental Mutation 'R4332:Gpr149'
| ID |
323613 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr149
|
| Ensembl Gene |
ENSMUSG00000043441 |
| Gene Name |
G protein-coupled receptor 149 |
| Synonyms |
PGR10, 9630018L10Rik, R35, Ieda |
| MMRRC Submission |
041099-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.074)
|
| Stock # |
R4332 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
3 |
| Chromosomal Location |
62436851-62512861 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to A
at 62511794 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Leucine to Phenylalanine
at position 68
(L68F)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000060893
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000058535]
|
| AlphaFold |
Q3UVY1 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000058535
AA Change: L68F
PolyPhen 2
Score 0.930 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000060893
Gene: ENSMUSG00000043441
AA Change: L68F
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_1
|
52 |
363 |
7.2e-7 |
PFAM |
|
coiled coil region
|
694 |
730 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000149007
|
| Meta Mutation Damage Score |
0.1795 |
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.4%
- 20x: 95.5%
|
| Validation Efficiency |
98% (56/57) |
| MGI Phenotype |
PHENOTYPE: Female mice homozygous for a knock-out allele exhibit increased fertility with increased litter size and frequency. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 47 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4933405L10Rik |
T |
C |
8: 106,436,356 (GRCm39) |
I175T |
possibly damaging |
Het |
| A2m |
A |
G |
6: 121,634,406 (GRCm39) |
D646G |
probably benign |
Het |
| Acat2 |
T |
C |
17: 13,181,782 (GRCm39) |
|
probably benign |
Het |
| Armc10 |
T |
A |
5: 21,866,579 (GRCm39) |
V281E |
probably damaging |
Het |
| Best3 |
T |
A |
10: 116,838,429 (GRCm39) |
F162L |
probably benign |
Het |
| Ces1g |
T |
C |
8: 94,046,446 (GRCm39) |
M360V |
probably benign |
Het |
| Chd7 |
G |
T |
4: 8,854,143 (GRCm39) |
R1905L |
probably damaging |
Het |
| Dhx36 |
C |
T |
3: 62,392,412 (GRCm39) |
R538Q |
probably damaging |
Het |
| Efna2 |
G |
A |
10: 80,024,315 (GRCm39) |
R161Q |
probably damaging |
Het |
| Farsb |
T |
C |
1: 78,445,903 (GRCm39) |
T159A |
possibly damaging |
Het |
| Fry |
C |
T |
5: 150,305,128 (GRCm39) |
A611V |
probably damaging |
Het |
| Fsip2 |
A |
G |
2: 82,808,201 (GRCm39) |
T1507A |
probably benign |
Het |
| Gm5592 |
G |
T |
7: 40,865,542 (GRCm39) |
|
probably benign |
Het |
| Gm7367 |
T |
C |
7: 59,805,364 (GRCm39) |
|
noncoding transcript |
Het |
| Gm9312 |
A |
T |
12: 24,302,095 (GRCm39) |
|
noncoding transcript |
Het |
| Gmfg |
A |
T |
7: 28,136,997 (GRCm39) |
M1L |
probably benign |
Het |
| Hmga2 |
T |
C |
10: 120,200,117 (GRCm39) |
|
probably benign |
Het |
| Il12a |
C |
A |
3: 68,602,594 (GRCm39) |
|
probably benign |
Het |
| Itprid1 |
G |
A |
6: 55,945,220 (GRCm39) |
G647D |
possibly damaging |
Het |
| Itsn2 |
T |
A |
12: 4,762,611 (GRCm39) |
M1597K |
possibly damaging |
Het |
| Kyat3 |
A |
G |
3: 142,431,187 (GRCm39) |
I154M |
probably damaging |
Het |
| Npas3 |
A |
C |
12: 54,108,852 (GRCm39) |
I419L |
probably damaging |
Het |
| Ogfrl1 |
T |
A |
1: 23,414,910 (GRCm39) |
Y199F |
probably damaging |
Het |
| Or14a259 |
A |
C |
7: 86,013,080 (GRCm39) |
V155G |
probably benign |
Het |
| Or5aq7 |
A |
G |
2: 86,938,089 (GRCm39) |
V214A |
possibly damaging |
Het |
| Or6b6 |
T |
C |
7: 106,571,354 (GRCm39) |
M66V |
probably benign |
Het |
| P2rx3 |
G |
A |
2: 84,855,205 (GRCm39) |
P84S |
probably benign |
Het |
| P3h3 |
A |
G |
6: 124,819,099 (GRCm39) |
V657A |
probably damaging |
Het |
| Pabpc2 |
A |
G |
18: 39,908,393 (GRCm39) |
M553V |
probably benign |
Het |
| Pcdhb1 |
T |
C |
18: 37,398,583 (GRCm39) |
F178S |
probably damaging |
Het |
| Plppr4 |
A |
T |
3: 117,116,474 (GRCm39) |
M403K |
probably benign |
Het |
| Ralgapa2 |
G |
A |
2: 146,102,288 (GRCm39) |
T1956M |
probably benign |
Het |
| Rbm12b1 |
G |
T |
4: 12,145,655 (GRCm39) |
K542N |
probably benign |
Het |
| Rdh8 |
C |
T |
9: 20,733,925 (GRCm39) |
A37V |
probably damaging |
Het |
| Rnf213 |
A |
G |
11: 119,327,502 (GRCm39) |
T1830A |
probably damaging |
Het |
| Sardh |
G |
T |
2: 27,105,126 (GRCm39) |
Q666K |
possibly damaging |
Het |
| Secisbp2l |
C |
T |
2: 125,582,657 (GRCm39) |
G933D |
possibly damaging |
Het |
| Septin4 |
T |
G |
11: 87,458,730 (GRCm39) |
L368R |
possibly damaging |
Het |
| Serpinb11 |
G |
A |
1: 107,297,294 (GRCm39) |
|
probably null |
Het |
| Slc6a6 |
G |
A |
6: 91,700,452 (GRCm39) |
G60D |
probably damaging |
Het |
| Tfr2 |
A |
G |
5: 137,569,996 (GRCm39) |
D134G |
probably damaging |
Het |
| Tmprss15 |
T |
C |
16: 78,831,222 (GRCm39) |
T378A |
probably benign |
Het |
| Tmprss7 |
T |
G |
16: 45,506,690 (GRCm39) |
K124T |
probably benign |
Het |
| Urb1 |
A |
G |
16: 90,571,425 (GRCm39) |
L1128P |
probably damaging |
Het |
| Usp32 |
C |
T |
11: 84,994,804 (GRCm39) |
C36Y |
possibly damaging |
Het |
| Vmn2r50 |
T |
A |
7: 9,786,922 (GRCm39) |
T62S |
probably benign |
Het |
| Zfp110 |
T |
A |
7: 12,578,498 (GRCm39) |
Y136* |
probably null |
Het |
|
| Other mutations in Gpr149 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00330:Gpr149
|
APN |
3 |
62,438,094 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr149
|
APN |
3 |
62,511,718 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01399:Gpr149
|
APN |
3 |
62,511,852 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Gpr149
|
APN |
3 |
62,438,348 (GRCm39) |
missense |
probably benign |
0.36 |
|
IGL02115:Gpr149
|
APN |
3 |
62,502,336 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL02218:Gpr149
|
APN |
3 |
62,437,952 (GRCm39) |
utr 3 prime |
probably benign |
|
|
IGL02592:Gpr149
|
APN |
3 |
62,511,231 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
IGL03393:Gpr149
|
APN |
3 |
62,511,366 (GRCm39) |
missense |
probably benign |
0.15 |
|
R0578:Gpr149
|
UTSW |
3 |
62,510,110 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R1173:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1174:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1175:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1432:Gpr149
|
UTSW |
3 |
62,438,439 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1484:Gpr149
|
UTSW |
3 |
62,502,592 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1972:Gpr149
|
UTSW |
3 |
62,438,216 (GRCm39) |
missense |
probably benign |
0.39 |
|
R1973:Gpr149
|
UTSW |
3 |
62,438,216 (GRCm39) |
missense |
probably benign |
0.39 |
|
R2180:Gpr149
|
UTSW |
3 |
62,511,489 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2241:Gpr149
|
UTSW |
3 |
62,511,474 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3118:Gpr149
|
UTSW |
3 |
62,502,443 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3547:Gpr149
|
UTSW |
3 |
62,502,549 (GRCm39) |
missense |
probably benign |
0.01 |
|
R3548:Gpr149
|
UTSW |
3 |
62,502,549 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4206:Gpr149
|
UTSW |
3 |
62,511,924 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4531:Gpr149
|
UTSW |
3 |
62,510,099 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4557:Gpr149
|
UTSW |
3 |
62,511,918 (GRCm39) |
missense |
probably benign |
0.02 |
|
R4557:Gpr149
|
UTSW |
3 |
62,438,291 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4593:Gpr149
|
UTSW |
3 |
62,510,151 (GRCm39) |
intron |
probably benign |
|
|
R5397:Gpr149
|
UTSW |
3 |
62,438,226 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6592:Gpr149
|
UTSW |
3 |
62,437,961 (GRCm39) |
missense |
probably benign |
0.02 |
|
R6642:Gpr149
|
UTSW |
3 |
62,437,995 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6845:Gpr149
|
UTSW |
3 |
62,511,942 (GRCm39) |
missense |
possibly damaging |
0.58 |
|
R7303:Gpr149
|
UTSW |
3 |
62,502,491 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
R7659:Gpr149
|
UTSW |
3 |
62,511,256 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7682:Gpr149
|
UTSW |
3 |
62,438,160 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7803:Gpr149
|
UTSW |
3 |
62,438,136 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7904:Gpr149
|
UTSW |
3 |
62,502,356 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7943:Gpr149
|
UTSW |
3 |
62,438,132 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8844:Gpr149
|
UTSW |
3 |
62,502,572 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8919:Gpr149
|
UTSW |
3 |
62,438,478 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9043:Gpr149
|
UTSW |
3 |
62,511,360 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9209:Gpr149
|
UTSW |
3 |
62,511,093 (GRCm39) |
missense |
probably benign |
0.40 |
|
Z1177:Gpr149
|
UTSW |
3 |
62,511,380 (GRCm39) |
frame shift |
probably null |
|
|
Z1190:Gpr149
|
UTSW |
3 |
62,511,972 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- GAGCTTGACTGACTCTCCAC -3'
(R):5'- GACTCTAACCTGTGGAAAGCAAG -3'
Sequencing Primer
(F):5'- CACCGTCCTGTTCATCGTATAAAAG -3'
(R):5'- GCAAGTCATAATTCTACGGAGAC -3'
|
| Posted On |
2015-06-24 |
Incidental Mutation