Mutagenetix > Incidental Mutation

Incidental Mutation 'R4332:Or6b6'

323634

Institutional Source

Beutler Lab

Gene Symbol

Or6b6

Ensembl Gene

ENSMUSG00000045013

Gene Name

olfactory receptor family 6 subfamily B member 6

Synonyms

MOR103-4, Olfr711, GA_x6K02T2PBJ9-9352783-9351839

MMRRC Submission

041099-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R4332 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

106570575-106574658 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 106571354 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 66 (M66V)

Ref Sequence

ENSEMBL: ENSMUSP00000149016 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051715] [ENSMUST00000207200] [ENSMUST00000216335]

AlphaFold

Q9EPG2

Predicted Effect

probably benign
Transcript: ENSMUST00000051715
AA Change: M66V

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000055724
Gene: ENSMUSG00000045013
AA Change: M66V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.5e-58	PFAM
Pfam:7tm_1	41	290	1.2e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000207200
AA Change: M66V

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

Predicted Effect

probably benign
Transcript: ENSMUST00000216335
AA Change: M66V

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

Meta Mutation Damage Score

0.1791

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

98% (56/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933405L10Rik	T	C	8: 106,436,356 (GRCm39)	I175T	possibly damaging	Het
A2m	A	G	6: 121,634,406 (GRCm39)	D646G	probably benign	Het
Acat2	T	C	17: 13,181,782 (GRCm39)		probably benign	Het
Armc10	T	A	5: 21,866,579 (GRCm39)	V281E	probably damaging	Het
Best3	T	A	10: 116,838,429 (GRCm39)	F162L	probably benign	Het
Ces1g	T	C	8: 94,046,446 (GRCm39)	M360V	probably benign	Het
Chd7	G	T	4: 8,854,143 (GRCm39)	R1905L	probably damaging	Het
Dhx36	C	T	3: 62,392,412 (GRCm39)	R538Q	probably damaging	Het
Efna2	G	A	10: 80,024,315 (GRCm39)	R161Q	probably damaging	Het
Farsb	T	C	1: 78,445,903 (GRCm39)	T159A	possibly damaging	Het
Fry	C	T	5: 150,305,128 (GRCm39)	A611V	probably damaging	Het
Fsip2	A	G	2: 82,808,201 (GRCm39)	T1507A	probably benign	Het
Gm5592	G	T	7: 40,865,542 (GRCm39)		probably benign	Het
Gm7367	T	C	7: 59,805,364 (GRCm39)		noncoding transcript	Het
Gm9312	A	T	12: 24,302,095 (GRCm39)		noncoding transcript	Het
Gmfg	A	T	7: 28,136,997 (GRCm39)	M1L	probably benign	Het
Gpr149	C	A	3: 62,511,794 (GRCm39)	L68F	possibly damaging	Het
Hmga2	T	C	10: 120,200,117 (GRCm39)		probably benign	Het
Il12a	C	A	3: 68,602,594 (GRCm39)		probably benign	Het
Itprid1	G	A	6: 55,945,220 (GRCm39)	G647D	possibly damaging	Het
Itsn2	T	A	12: 4,762,611 (GRCm39)	M1597K	possibly damaging	Het
Kyat3	A	G	3: 142,431,187 (GRCm39)	I154M	probably damaging	Het
Npas3	A	C	12: 54,108,852 (GRCm39)	I419L	probably damaging	Het
Ogfrl1	T	A	1: 23,414,910 (GRCm39)	Y199F	probably damaging	Het
Or14a259	A	C	7: 86,013,080 (GRCm39)	V155G	probably benign	Het
Or5aq7	A	G	2: 86,938,089 (GRCm39)	V214A	possibly damaging	Het
P2rx3	G	A	2: 84,855,205 (GRCm39)	P84S	probably benign	Het
P3h3	A	G	6: 124,819,099 (GRCm39)	V657A	probably damaging	Het
Pabpc2	A	G	18: 39,908,393 (GRCm39)	M553V	probably benign	Het
Pcdhb1	T	C	18: 37,398,583 (GRCm39)	F178S	probably damaging	Het
Plppr4	A	T	3: 117,116,474 (GRCm39)	M403K	probably benign	Het
Ralgapa2	G	A	2: 146,102,288 (GRCm39)	T1956M	probably benign	Het
Rbm12b1	G	T	4: 12,145,655 (GRCm39)	K542N	probably benign	Het
Rdh8	C	T	9: 20,733,925 (GRCm39)	A37V	probably damaging	Het
Rnf213	A	G	11: 119,327,502 (GRCm39)	T1830A	probably damaging	Het
Sardh	G	T	2: 27,105,126 (GRCm39)	Q666K	possibly damaging	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Septin4	T	G	11: 87,458,730 (GRCm39)	L368R	possibly damaging	Het
Serpinb11	G	A	1: 107,297,294 (GRCm39)		probably null	Het
Slc6a6	G	A	6: 91,700,452 (GRCm39)	G60D	probably damaging	Het
Tfr2	A	G	5: 137,569,996 (GRCm39)	D134G	probably damaging	Het
Tmprss15	T	C	16: 78,831,222 (GRCm39)	T378A	probably benign	Het
Tmprss7	T	G	16: 45,506,690 (GRCm39)	K124T	probably benign	Het
Urb1	A	G	16: 90,571,425 (GRCm39)	L1128P	probably damaging	Het
Usp32	C	T	11: 84,994,804 (GRCm39)	C36Y	possibly damaging	Het
Vmn2r50	T	A	7: 9,786,922 (GRCm39)	T62S	probably benign	Het
Zfp110	T	A	7: 12,578,498 (GRCm39)	Y136*	probably null	Het

Other mutations in Or6b6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02600:Or6b6	APN	7	106,570,756 (GRCm39)	missense	possibly damaging	0.51
R0087:Or6b6	UTSW	7	106,571,323 (GRCm39)	missense	probably benign	0.01
R0580:Or6b6	UTSW	7	106,571,447 (GRCm39)	missense	probably damaging	1.00
R1375:Or6b6	UTSW	7	106,571,305 (GRCm39)	missense	probably damaging	1.00
R1538:Or6b6	UTSW	7	106,571,190 (GRCm39)	nonsense	probably null
R1875:Or6b6	UTSW	7	106,571,389 (GRCm39)	missense	possibly damaging	0.86
R2156:Or6b6	UTSW	7	106,570,775 (GRCm39)	missense	probably damaging	1.00
R4290:Or6b6	UTSW	7	106,570,918 (GRCm39)	missense	probably damaging	0.97
R4400:Or6b6	UTSW	7	106,571,209 (GRCm39)	missense	probably damaging	1.00
R4688:Or6b6	UTSW	7	106,571,068 (GRCm39)	missense	probably benign	0.02
R4868:Or6b6	UTSW	7	106,570,974 (GRCm39)	missense	probably benign
R4970:Or6b6	UTSW	7	106,570,778 (GRCm39)	missense	probably benign	0.35
R5006:Or6b6	UTSW	7	106,570,808 (GRCm39)	missense	probably damaging	1.00
R5082:Or6b6	UTSW	7	106,570,871 (GRCm39)	missense	probably benign	0.00
R5121:Or6b6	UTSW	7	106,571,438 (GRCm39)	missense	probably benign
R6465:Or6b6	UTSW	7	106,571,419 (GRCm39)	missense	possibly damaging	0.63
R6541:Or6b6	UTSW	7	106,571,410 (GRCm39)	missense	probably benign	0.20
R7419:Or6b6	UTSW	7	106,571,353 (GRCm39)	missense	probably benign	0.01
R8048:Or6b6	UTSW	7	106,571,671 (GRCm39)	start gained	probably benign
R9310:Or6b6	UTSW	7	106,570,678 (GRCm39)	missense	probably damaging	1.00
R9470:Or6b6	UTSW	7	106,571,461 (GRCm39)	missense	probably benign	0.26
R9603:Or6b6	UTSW	7	106,571,103 (GRCm39)	nonsense	probably null
Z1177:Or6b6	UTSW	7	106,571,122 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATGGCCACATAGCGGTCATAG -3'
(R):5'- AATATCTCCCAGTGCCTTTGG -3'

Sequencing Primer
(F):5'- CATAGCGGTCATAGGCCATAGCTG -3'
(R):5'- GCTGGATATGAACATTACTCTGGTC -3'

Posted On

2015-06-24