Mutagenetix > Incidental Mutation

Incidental Mutation 'R4323:Vmn2r63'

323914

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

041094-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R4323 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42552675-42583213 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 42576406 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 469 (F469S)

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803]

AlphaFold

E9Q0K5

Predicted Effect

probably benign
Transcript: ENSMUST00000163803
AA Change: F469S

PolyPhen 2 Score 0.040 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751
AA Change: F469S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

95% (59/62)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933430I17Rik	A	G	4: 62,465,548 (GRCm39)	Y414C	probably damaging	Het
Akr1b1	T	C	6: 34,287,862 (GRCm39)	T166A	probably benign	Het
Ankhd1	C	T	18: 36,711,686 (GRCm39)	S94L	probably damaging	Het
B4galt3	G	T	1: 171,103,515 (GRCm39)	M68I	possibly damaging	Het
Bpnt1	A	C	1: 185,088,786 (GRCm39)	H312P	probably benign	Het
Ccdc178	T	A	18: 22,166,600 (GRCm39)	K530*	probably null	Het
Ccdc191	A	G	16: 43,767,872 (GRCm39)	E624G	probably damaging	Het
Clasp2	T	C	9: 113,719,027 (GRCm39)	V724A	possibly damaging	Het
Copa	G	T	1: 171,946,831 (GRCm39)	C1022F	probably damaging	Het
Cwf19l2	T	G	9: 3,430,452 (GRCm39)	F261L	probably damaging	Het
Esr1	G	A	10: 4,951,307 (GRCm39)	V562M	possibly damaging	Het
Fap	T	C	2: 62,333,716 (GRCm39)	H643R	probably damaging	Het
Fbxo38	T	A	18: 62,648,232 (GRCm39)	M769L	probably benign	Het
Fgfr1	A	G	8: 26,063,915 (GRCm39)	N814S	probably benign	Het
Hipk3	C	A	2: 104,276,916 (GRCm39)	V388L	probably damaging	Het
Hspa1l	T	A	17: 35,196,832 (GRCm39)	Y290*	probably null	Het
Itsn1	G	A	16: 91,615,440 (GRCm39)		probably benign	Het
Jam2	T	C	16: 84,619,744 (GRCm39)		probably benign	Het
Kprp	G	A	3: 92,732,163 (GRCm39)	R296W	probably damaging	Het
Med23	T	C	10: 24,746,603 (GRCm39)	I14T	probably benign	Het
Mitf	A	G	6: 97,968,910 (GRCm39)	Y10C	probably benign	Het
Mpo	T	C	11: 87,686,865 (GRCm39)	S165P	probably damaging	Het
Neb	T	G	2: 52,154,122 (GRCm39)	M2330L	possibly damaging	Het
Nup214	T	C	2: 31,884,696 (GRCm39)	S486P	probably benign	Het
Or2y1f	G	A	11: 49,184,503 (GRCm39)	M118I	probably damaging	Het
Parp6	G	A	9: 59,537,969 (GRCm39)	V205I	possibly damaging	Het
Pate2	A	T	9: 35,581,767 (GRCm39)		probably benign	Het
Pdss1	T	C	2: 22,802,608 (GRCm39)		probably benign	Het
Pira13	A	G	7: 3,825,754 (GRCm39)	S372P	possibly damaging	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Septin1	G	A	7: 126,816,200 (GRCm39)	P77S	probably damaging	Het
Slitrk3	A	G	3: 72,958,118 (GRCm39)	L218P	probably damaging	Het
Sltm	T	C	9: 70,487,529 (GRCm39)	I521T	probably benign	Het
Smchd1	A	G	17: 71,735,270 (GRCm39)	I618T	probably benign	Het
Sox6	A	G	7: 115,179,798 (GRCm39)		probably null	Het
Sp8	A	G	12: 118,812,171 (GRCm39)	I9V	probably benign	Het
Usp9y	T	A	Y: 1,434,407 (GRCm39)	M352L	possibly damaging	Het
Vmn1r46	T	C	6: 89,953,349 (GRCm39)	M66T	probably benign	Het
Vmn2r111	A	T	17: 22,792,159 (GRCm39)	N32K	probably benign	Het
Vps13d	T	A	4: 144,879,348 (GRCm39)	T1486S	probably benign	Het
Wdr55	A	G	18: 36,896,153 (GRCm39)	N281S	probably benign	Het
Zswim6	G	A	13: 108,025,938 (GRCm39)		noncoding transcript	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42,553,543 (GRCm39)	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42,552,788 (GRCm39)	missense	probably benign
IGL02203:Vmn2r63	APN	7	42,553,432 (GRCm39)	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42,576,274 (GRCm39)	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42,552,878 (GRCm39)	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42,578,616 (GRCm39)	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42,577,368 (GRCm39)	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42,553,409 (GRCm39)	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42,576,514 (GRCm39)	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42,552,699 (GRCm39)	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42,553,042 (GRCm39)	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42,583,129 (GRCm39)	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42,577,952 (GRCm39)	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42,577,434 (GRCm39)	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42,577,459 (GRCm39)	missense	probably damaging	1.00
R1349:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1372:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42,577,339 (GRCm39)	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42,578,015 (GRCm39)	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42,553,550 (GRCm39)	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42,577,743 (GRCm39)	missense	probably benign
R1698:Vmn2r63	UTSW	7	42,583,038 (GRCm39)	missense	probably benign
R1753:Vmn2r63	UTSW	7	42,577,669 (GRCm39)	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42,576,297 (GRCm39)	missense	probably damaging	0.99
R2175:Vmn2r63	UTSW	7	42,583,004 (GRCm39)	critical splice donor site	probably null
R2261:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2263:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3427:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42,552,829 (GRCm39)	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42,577,537 (GRCm39)	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42,583,250 (GRCm39)	splice site	probably null
R4649:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42,576,314 (GRCm39)	missense	probably benign	0.34
R4933:Vmn2r63	UTSW	7	42,553,402 (GRCm39)	missense	probably damaging	1.00
R5198:Vmn2r63	UTSW	7	42,553,169 (GRCm39)	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42,577,701 (GRCm39)	missense	probably benign
R5400:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R6158:Vmn2r63	UTSW	7	42,583,104 (GRCm39)	missense	probably damaging	1.00
R6185:Vmn2r63	UTSW	7	42,578,435 (GRCm39)	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42,578,059 (GRCm39)	splice site	probably null
R6362:Vmn2r63	UTSW	7	42,552,721 (GRCm39)	missense	probably benign
R6706:Vmn2r63	UTSW	7	42,578,001 (GRCm39)	missense	probably damaging	1.00
R6764:Vmn2r63	UTSW	7	42,552,695 (GRCm39)	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42,577,959 (GRCm39)	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42,583,014 (GRCm39)	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42,576,391 (GRCm39)	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42,574,693 (GRCm39)	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42,576,466 (GRCm39)	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42,577,553 (GRCm39)	missense	possibly damaging	0.46
R7918:Vmn2r63	UTSW	7	42,552,955 (GRCm39)	missense	probably damaging	1.00
R8826:Vmn2r63	UTSW	7	42,576,441 (GRCm39)	missense	probably benign	0.38
R8973:Vmn2r63	UTSW	7	42,577,919 (GRCm39)	missense	probably benign	0.19
R9024:Vmn2r63	UTSW	7	42,577,874 (GRCm39)	missense	probably benign	0.14
R9154:Vmn2r63	UTSW	7	42,576,413 (GRCm39)	missense	probably damaging	0.96
R9731:Vmn2r63	UTSW	7	42,553,361 (GRCm39)	missense	probably benign	0.32
Z1088:Vmn2r63	UTSW	7	42,577,983 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- GAGTGTCCAATTAACACCATATCCTG -3'
(R):5'- TTCATCATGAGGGGATAAAAGACAC -3'

Sequencing Primer
(F):5'- GTGTCGGCCATTGTATCA -3'
(R):5'- GGGATAAAAGACACTGAGATTGTTTG -3'

Posted On

2015-06-24