Mutagenetix > Incidental Mutation

Incidental Mutation 'R4324:Vmn2r52'

323962

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r52

Ensembl Gene

ENSMUSG00000091930

Gene Name

vomeronasal 2, receptor 52

Synonyms

EG384534

MMRRC Submission

041095-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R4324 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

9892579-9910213 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 9904940 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 300 (T300S)

Ref Sequence

ENSEMBL: ENSMUSP00000129352 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164918]

AlphaFold

L7N2B2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000164918
AA Change: T300S

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000129352
Gene: ENSMUSG00000091930
AA Change: T300S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	469	8.1e-29	PFAM
Pfam:NCD3G	512	565	1.5e-19	PFAM
Pfam:7tm_3	596	833	1.1e-55	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Afp	A	G	5: 90,655,764 (GRCm39)	D583G	probably benign	Het
Alg9	T	G	9: 50,716,643 (GRCm39)	H22Q	probably damaging	Het
Aqp6	G	A	15: 99,499,291 (GRCm39)	M1I	probably null	Het
Arhgef10	T	A	8: 14,990,335 (GRCm39)	I270N	possibly damaging	Het
Asb13	G	T	13: 3,695,012 (GRCm39)	R160L	possibly damaging	Het
Atg10	T	A	13: 91,189,085 (GRCm39)	D75V	probably damaging	Het
Atxn7l2	A	T	3: 108,113,148 (GRCm39)	D218E	probably damaging	Het
Ccdc191	A	G	16: 43,767,872 (GRCm39)	E624G	probably damaging	Het
Cdan1	T	C	2: 120,555,460 (GRCm39)	I760M	probably damaging	Het
Cdk14	T	A	5: 5,086,532 (GRCm39)	K263*	probably null	Het
Cdk5rap2	A	T	4: 70,271,851 (GRCm39)	I287N	probably damaging	Het
Cdkn2aip	A	T	8: 48,165,208 (GRCm39)	S168R	probably benign	Het
Dnah17	C	T	11: 117,985,039 (GRCm39)	V1555M	probably benign	Het
Dsel	T	C	1: 111,789,123 (GRCm39)	T471A	probably damaging	Het
Dzank1	T	C	2: 144,330,618 (GRCm39)	E478G	possibly damaging	Het
Enc1	T	C	13: 97,382,405 (GRCm39)	F305S	probably benign	Het
Epn1	T	A	7: 5,100,210 (GRCm39)	M441K	probably benign	Het
Fbxw24	C	T	9: 109,434,013 (GRCm39)		probably null	Het
Gad1	G	A	2: 70,420,174 (GRCm39)	D353N	probably damaging	Het
Ggcx	A	G	6: 72,405,803 (GRCm39)	S545G	probably benign	Het
H2-K2	C	T	17: 34,219,014 (GRCm39)	V30M	possibly damaging	Het
Hipk3	C	A	2: 104,276,916 (GRCm39)	V388L	probably damaging	Het
Krt82	T	A	15: 101,450,182 (GRCm39)	M505L	probably benign	Het
Man2a1	A	G	17: 64,973,788 (GRCm39)	I355V	probably benign	Het
Or5p53	C	T	7: 107,532,900 (GRCm39)	P58S	probably damaging	Het
Pcbp3	G	A	10: 76,599,177 (GRCm39)	R109*	probably null	Het
Pcgf1	T	C	6: 83,056,938 (GRCm39)		probably null	Het
Plekhm2	A	T	4: 141,359,168 (GRCm39)	V533E	possibly damaging	Het
Prss3	T	G	6: 41,350,779 (GRCm39)	D237A	probably benign	Het
Rnf125	G	A	18: 21,110,817 (GRCm39)	R25K	probably benign	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Shc4	T	A	2: 125,520,670 (GRCm39)	M260L	probably benign	Het
Spag6l	T	C	16: 16,605,099 (GRCm39)	Y151C	probably benign	Het
Stac3	C	T	10: 127,339,118 (GRCm39)	P76S	probably damaging	Het
Trpc7	T	C	13: 57,035,169 (GRCm39)	I255V	probably damaging	Het
Ttn	T	C	2: 76,738,923 (GRCm39)	E3872G	probably benign	Het
Uqcrfs1	A	G	13: 30,725,141 (GRCm39)	V133A	probably benign	Het
Wipi1	A	T	11: 109,494,662 (GRCm39)	I57N	possibly damaging	Het

Other mutations in Vmn2r52

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r52	APN	7	9,903,023 (GRCm39)	missense	probably benign	0.30
IGL00328:Vmn2r52	APN	7	9,905,344 (GRCm39)	missense	probably benign	0.12
IGL00980:Vmn2r52	APN	7	9,905,017 (GRCm39)	missense	probably damaging	1.00
IGL01468:Vmn2r52	APN	7	9,892,868 (GRCm39)	missense	probably damaging	1.00
IGL01660:Vmn2r52	APN	7	9,893,107 (GRCm39)	missense	probably damaging	0.97
IGL02215:Vmn2r52	APN	7	9,905,029 (GRCm39)	missense	probably damaging	0.97
IGL03030:Vmn2r52	APN	7	9,892,799 (GRCm39)	missense	probably benign	0.12
IGL03212:Vmn2r52	APN	7	9,893,474 (GRCm39)	missense	possibly damaging	0.47
FR4589:Vmn2r52	UTSW	7	9,892,947 (GRCm39)	missense	probably damaging	0.97
PIT4283001:Vmn2r52	UTSW	7	9,904,756 (GRCm39)	missense	possibly damaging	0.89
R0184:Vmn2r52	UTSW	7	9,893,265 (GRCm39)	missense	probably damaging	1.00
R0190:Vmn2r52	UTSW	7	9,905,315 (GRCm39)	missense	probably benign	0.00
R0240:Vmn2r52	UTSW	7	9,893,327 (GRCm39)	missense	probably damaging	0.99
R0240:Vmn2r52	UTSW	7	9,893,327 (GRCm39)	missense	probably damaging	0.99
R0257:Vmn2r52	UTSW	7	9,904,982 (GRCm39)	nonsense	probably null
R0310:Vmn2r52	UTSW	7	9,893,393 (GRCm39)	missense	probably damaging	1.00
R1831:Vmn2r52	UTSW	7	9,893,415 (GRCm39)	missense	probably damaging	1.00
R1862:Vmn2r52	UTSW	7	9,907,333 (GRCm39)	missense	possibly damaging	0.94
R2484:Vmn2r52	UTSW	7	9,903,058 (GRCm39)	missense	probably damaging	0.96
R2510:Vmn2r52	UTSW	7	9,904,795 (GRCm39)	missense	probably benign
R3625:Vmn2r52	UTSW	7	9,893,105 (GRCm39)	missense	probably damaging	1.00
R3803:Vmn2r52	UTSW	7	9,907,439 (GRCm39)	missense	probably damaging	1.00
R4013:Vmn2r52	UTSW	7	9,904,603 (GRCm39)	missense	probably benign	0.00
R4283:Vmn2r52	UTSW	7	9,904,565 (GRCm39)	missense	possibly damaging	0.60
R4578:Vmn2r52	UTSW	7	9,904,617 (GRCm39)	missense	probably damaging	1.00
R4806:Vmn2r52	UTSW	7	9,893,169 (GRCm39)	missense	probably damaging	1.00
R5083:Vmn2r52	UTSW	7	9,893,392 (GRCm39)	nonsense	probably null
R5249:Vmn2r52	UTSW	7	9,910,197 (GRCm39)	missense	probably benign
R5306:Vmn2r52	UTSW	7	9,904,672 (GRCm39)	missense	possibly damaging	0.88
R5332:Vmn2r52	UTSW	7	9,903,052 (GRCm39)	missense	probably benign	0.17
R5617:Vmn2r52	UTSW	7	9,904,861 (GRCm39)	missense	probably damaging	0.99
R5643:Vmn2r52	UTSW	7	9,905,059 (GRCm39)	missense	probably damaging	1.00
R5749:Vmn2r52	UTSW	7	9,892,959 (GRCm39)	missense	probably damaging	1.00
R5763:Vmn2r52	UTSW	7	9,905,231 (GRCm39)	missense	probably benign	0.01
R6103:Vmn2r52	UTSW	7	9,905,327 (GRCm39)	missense	probably benign	0.36
R6148:Vmn2r52	UTSW	7	9,905,090 (GRCm39)	missense	probably benign	0.00
R6356:Vmn2r52	UTSW	7	9,902,926 (GRCm39)	missense	probably benign	0.01
R6412:Vmn2r52	UTSW	7	9,904,936 (GRCm39)	missense	probably benign
R6657:Vmn2r52	UTSW	7	9,893,090 (GRCm39)	missense	probably damaging	0.99
R6997:Vmn2r52	UTSW	7	9,902,998 (GRCm39)	missense	probably benign	0.06
R7395:Vmn2r52	UTSW	7	9,904,744 (GRCm39)	missense	probably benign	0.00
R7621:Vmn2r52	UTSW	7	9,907,274 (GRCm39)	missense	probably benign	0.00
R7691:Vmn2r52	UTSW	7	9,893,109 (GRCm39)	missense	probably damaging	0.97
R7852:Vmn2r52	UTSW	7	9,892,895 (GRCm39)	missense	probably damaging	1.00
R7908:Vmn2r52	UTSW	7	9,896,877 (GRCm39)	missense	probably benign
R7909:Vmn2r52	UTSW	7	9,896,877 (GRCm39)	missense	probably benign
R7912:Vmn2r52	UTSW	7	9,896,877 (GRCm39)	missense	probably benign
R7913:Vmn2r52	UTSW	7	9,896,877 (GRCm39)	missense	probably benign
R7938:Vmn2r52	UTSW	7	9,893,300 (GRCm39)	missense	probably benign	0.12
R8884:Vmn2r52	UTSW	7	9,892,734 (GRCm39)	missense	probably damaging	1.00
R9003:Vmn2r52	UTSW	7	9,905,181 (GRCm39)	missense	probably benign	0.07
R9140:Vmn2r52	UTSW	7	9,892,643 (GRCm39)	missense	probably damaging	0.99
R9141:Vmn2r52	UTSW	7	9,905,331 (GRCm39)	nonsense	probably null
R9500:Vmn2r52	UTSW	7	9,905,281 (GRCm39)	missense	probably damaging	1.00
R9562:Vmn2r52	UTSW	7	9,893,476 (GRCm39)	missense	probably benign	0.22
R9564:Vmn2r52	UTSW	7	9,905,182 (GRCm39)	missense	probably benign	0.15
R9565:Vmn2r52	UTSW	7	9,893,476 (GRCm39)	missense	probably benign	0.22
R9597:Vmn2r52	UTSW	7	9,904,719 (GRCm39)	nonsense	probably null
R9743:Vmn2r52	UTSW	7	9,904,606 (GRCm39)	missense	possibly damaging	0.81
Z1176:Vmn2r52	UTSW	7	9,905,127 (GRCm39)	missense	probably damaging	0.97
Z1177:Vmn2r52	UTSW	7	9,903,117 (GRCm39)	missense	possibly damaging	0.93

Predicted Primers

PCR Primer
(F):5'- TTACAGTTACATGCTGAGTCTTCAC -3'
(R):5'- CAAGGAAATTTGCTTTGCCTTTGTG -3'

Sequencing Primer
(F):5'- CACAGTTAAAATATTTCCACTCTGGC -3'
(R):5'- GCCTTTGTGAAAATGATAGGAATCTC -3'

Posted On

2015-06-24