Mutagenetix > Incidental Mutation

Incidental Mutation 'R4378:Vmn2r88'

325212

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r88

Ensembl Gene

ENSMUSG00000000606

Gene Name

vomeronasal 2, receptor 88

Synonyms

V2r13, V2r3

MMRRC Submission

041121-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.110) question?

Stock #

R4378 (G1)

Quality Score

107

Status

Validated

Chromosome

Chromosomal Location

51648458-51656984 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 51650746 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 153 (L153*)

Ref Sequence

ENSEMBL: ENSMUSP00000154310 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022438] [ENSMUST00000159674] [ENSMUST00000162998] [ENSMUST00000228139]

AlphaFold

L7N1W8

Predicted Effect

probably null
Transcript: ENSMUST00000022438
AA Change: L153*

SMART Domains

Protein: ENSMUSP00000022438
Gene: ENSMUSG00000000606
AA Change: L153*

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	457	8.3e-27	PFAM
Pfam:NCD3G	516	570	1.2e-18	PFAM
Pfam:7tm_3	603	838	1.9e-55	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000159674
AA Change: L153*

SMART Domains

Protein: ENSMUSP00000125126
Gene: ENSMUSG00000000606
AA Change: L153*

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	30	408	3.2e-30	PFAM
Pfam:NCD3G	463	516	1.2e-19	PFAM
Pfam:7tm_3	546	785	3.7e-81	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161565

Predicted Effect

probably benign
Transcript: ENSMUST00000162998

SMART Domains

Protein: ENSMUSP00000125409
Gene: ENSMUSG00000068399

Domain	Start	End	E-Value	Type
Pfam:Takusan	35	115	2.2e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000163019
AA Change: L128*

SMART Domains

Protein: ENSMUSP00000124837
Gene: ENSMUSG00000000606
AA Change: L128*

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	52	399	3.7e-30	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000228139
AA Change: L153*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

96% (47/49)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	G	11: 9,243,644 (GRCm39)	K1836E	probably benign	Het
Adora1	A	G	1: 134,130,948 (GRCm39)	F241S	probably damaging	Het
Akt1s1	C	T	7: 44,503,384 (GRCm39)	T168M	probably damaging	Het
Amigo1	C	T	3: 108,099,069 (GRCm39)		probably benign	Het
Amigo2	A	G	15: 97,143,859 (GRCm39)	F188L	possibly damaging	Het
Armc2	T	C	10: 41,869,078 (GRCm39)	T29A	possibly damaging	Het
Axl	G	A	7: 25,458,262 (GRCm39)	A822V	probably benign	Het
Borcs5	T	C	6: 134,621,292 (GRCm39)	V21A	probably benign	Het
Cdc42ep3	A	G	17: 79,642,408 (GRCm39)	S171P	probably benign	Het
Cmas	T	C	6: 142,718,011 (GRCm39)		probably benign	Het
Cyp4f40	A	G	17: 32,887,003 (GRCm39)	N158S	probably null	Het
Dnah6	G	T	6: 73,095,009 (GRCm39)	N2139K	probably benign	Het
Elmo1	A	T	13: 20,557,286 (GRCm39)	H409L	possibly damaging	Het
Exoc1l	A	G	5: 76,648,380 (GRCm39)	K62R	probably damaging	Het
Exoc4	T	A	6: 33,792,622 (GRCm39)	V584E	probably damaging	Het
Gm9116	A	G	3: 93,817,786 (GRCm39)		noncoding transcript	Het
Gxylt2	A	G	6: 100,710,161 (GRCm39)	S101G	probably benign	Het
Hsph1	C	A	5: 149,559,472 (GRCm39)	E24*	probably null	Het
Ighv1-56	C	T	12: 115,206,568 (GRCm39)	E50K	probably benign	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Lrriq1	A	T	10: 103,038,225 (GRCm39)	D859E	probably damaging	Het
Mapk7	G	A	11: 61,384,493 (GRCm39)	S71L	probably damaging	Het
Nars1	A	G	18: 64,634,424 (GRCm39)	Y500H	probably damaging	Het
Or1j1	T	C	2: 36,702,481 (GRCm39)	M208V	probably benign	Het
Or5d20-ps1	G	T	2: 87,931,829 (GRCm39)	N167K	unknown	Het
Phc1	T	C	6: 122,311,966 (GRCm39)	N64S	possibly damaging	Het
Ppp4r3b	T	A	11: 29,159,450 (GRCm39)	N180K	possibly damaging	Het
Primpol	G	A	8: 47,029,218 (GRCm39)		probably benign	Het
Prtg	T	C	9: 72,750,042 (GRCm39)	S149P	possibly damaging	Het
Qrich2	T	C	11: 116,337,741 (GRCm39)	S1916G	probably damaging	Het
Rps6ka5	A	T	12: 100,564,196 (GRCm39)	Y218N	probably damaging	Het
Setbp1	T	C	18: 78,899,833 (GRCm39)	D1278G	possibly damaging	Het
Sirt1	C	T	10: 63,174,728 (GRCm39)	A8T	probably benign	Het
Sobp	C	T	10: 42,897,300 (GRCm39)	V762I	probably damaging	Het
Sptan1	T	C	2: 29,915,581 (GRCm39)	S1994P	probably damaging	Het
Taok3	T	C	5: 117,347,636 (GRCm39)	I87T	probably damaging	Het
Tecta	T	C	9: 42,278,004 (GRCm39)	Y1168C	probably damaging	Het
Trhr	A	G	15: 44,061,023 (GRCm39)	Y181C	probably damaging	Het
Ube4b	C	T	4: 149,468,255 (GRCm39)	D174N	probably damaging	Het
Ubr4	C	T	4: 139,137,751 (GRCm39)	H1067Y	possibly damaging	Het
Vmn2r86	A	G	10: 130,288,469 (GRCm39)	I344T	possibly damaging	Het
Zfp516	T	A	18: 83,005,305 (GRCm39)	D736E	probably benign	Het
Zfp612	G	T	8: 110,815,683 (GRCm39)	V258F	possibly damaging	Het

Other mutations in Vmn2r88

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00990:Vmn2r88	APN	14	51,650,713 (GRCm39)	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51,650,517 (GRCm39)	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51,650,582 (GRCm39)	missense	probably benign	0.00
IGL00990:Vmn2r88	APN	14	51,654,259 (GRCm39)	missense	possibly damaging	0.59
IGL02308:Vmn2r88	APN	14	51,655,437 (GRCm39)	missense	possibly damaging	0.96
IGL02481:Vmn2r88	APN	14	51,651,611 (GRCm39)	missense	probably benign
IGL02483:Vmn2r88	APN	14	51,651,611 (GRCm39)	missense	probably benign
IGL03241:Vmn2r88	APN	14	51,655,830 (GRCm39)	missense	probably benign	0.03
R0052:Vmn2r88	UTSW	14	51,656,157 (GRCm39)	missense	possibly damaging	0.88
R0070:Vmn2r88	UTSW	14	51,651,597 (GRCm39)	missense	probably benign	0.08
R0799:Vmn2r88	UTSW	14	51,651,959 (GRCm39)	missense	possibly damaging	0.61
R0906:Vmn2r88	UTSW	14	51,655,666 (GRCm39)	missense	probably damaging	1.00
R1322:Vmn2r88	UTSW	14	51,651,565 (GRCm39)	missense	probably damaging	1.00
R1352:Vmn2r88	UTSW	14	51,656,007 (GRCm39)	missense	probably damaging	1.00
R1639:Vmn2r88	UTSW	14	51,654,244 (GRCm39)	missense	probably damaging	0.98
R1780:Vmn2r88	UTSW	14	51,656,029 (GRCm39)	missense	probably damaging	1.00
R1834:Vmn2r88	UTSW	14	51,650,487 (GRCm39)	splice site	probably benign
R1911:Vmn2r88	UTSW	14	51,655,671 (GRCm39)	missense	probably damaging	1.00
R2113:Vmn2r88	UTSW	14	51,655,651 (GRCm39)	missense	probably damaging	1.00
R2120:Vmn2r88	UTSW	14	51,650,665 (GRCm39)	missense	probably benign	0.00
R2126:Vmn2r88	UTSW	14	51,651,264 (GRCm39)	missense	probably benign	0.01
R2348:Vmn2r88	UTSW	14	51,651,461 (GRCm39)	missense	probably benign	0.00
R2881:Vmn2r88	UTSW	14	51,656,146 (GRCm39)	missense	probably damaging	0.97
R2884:Vmn2r88	UTSW	14	51,651,391 (GRCm39)	missense	probably damaging	1.00
R3081:Vmn2r88	UTSW	14	51,656,089 (GRCm39)	missense	probably damaging	0.99
R3933:Vmn2r88	UTSW	14	51,651,435 (GRCm39)	missense	probably benign	0.44
R3967:Vmn2r88	UTSW	14	51,650,647 (GRCm39)	missense	probably benign	0.06
R4091:Vmn2r88	UTSW	14	51,652,883 (GRCm39)	missense	probably damaging	1.00
R4397:Vmn2r88	UTSW	14	51,655,435 (GRCm39)	missense	probably damaging	1.00
R4418:Vmn2r88	UTSW	14	51,655,538 (GRCm39)	missense	probably damaging	1.00
R4609:Vmn2r88	UTSW	14	51,655,531 (GRCm39)	missense	probably damaging	0.98
R4647:Vmn2r88	UTSW	14	51,656,250 (GRCm39)	missense	probably benign	0.02
R4672:Vmn2r88	UTSW	14	51,655,612 (GRCm39)	missense	probably damaging	1.00
R4684:Vmn2r88	UTSW	14	51,650,791 (GRCm39)	missense	possibly damaging	0.95
R4686:Vmn2r88	UTSW	14	51,650,796 (GRCm39)	missense	probably benign	0.03
R4720:Vmn2r88	UTSW	14	51,650,702 (GRCm39)	missense	probably benign	0.01
R5046:Vmn2r88	UTSW	14	51,650,638 (GRCm39)	missense	probably benign	0.03
R5063:Vmn2r88	UTSW	14	51,648,603 (GRCm39)	missense	probably damaging	0.96
R5619:Vmn2r88	UTSW	14	51,651,367 (GRCm39)	missense	probably damaging	0.99
R5652:Vmn2r88	UTSW	14	51,656,029 (GRCm39)	missense	probably damaging	0.98
R6020:Vmn2r88	UTSW	14	51,655,606 (GRCm39)	nonsense	probably null
R6103:Vmn2r88	UTSW	14	51,652,826 (GRCm39)	missense	probably benign	0.17
R6674:Vmn2r88	UTSW	14	51,651,795 (GRCm39)	missense	probably benign	0.01
R6799:Vmn2r88	UTSW	14	51,651,426 (GRCm39)	missense	probably benign	0.05
R7089:Vmn2r88	UTSW	14	51,656,100 (GRCm39)	missense
R7104:Vmn2r88	UTSW	14	51,651,253 (GRCm39)	missense
R7265:Vmn2r88	UTSW	14	51,655,776 (GRCm39)	missense
R7316:Vmn2r88	UTSW	14	51,651,712 (GRCm39)	missense
R7552:Vmn2r88	UTSW	14	51,648,315 (GRCm39)	splice site	probably null
R7611:Vmn2r88	UTSW	14	51,651,454 (GRCm39)	missense
R7667:Vmn2r88	UTSW	14	51,655,446 (GRCm39)	missense
R7682:Vmn2r88	UTSW	14	51,655,906 (GRCm39)	missense
R7755:Vmn2r88	UTSW	14	51,650,503 (GRCm39)	missense	probably benign	0.00
R7811:Vmn2r88	UTSW	14	51,656,160 (GRCm39)	missense
R7882:Vmn2r88	UTSW	14	51,650,503 (GRCm39)	missense	probably benign	0.00
R7957:Vmn2r88	UTSW	14	51,650,589 (GRCm39)	missense
R7998:Vmn2r88	UTSW	14	51,651,565 (GRCm39)	missense
R8142:Vmn2r88	UTSW	14	51,651,564 (GRCm39)	missense
R8186:Vmn2r88	UTSW	14	51,656,157 (GRCm39)	missense
R8348:Vmn2r88	UTSW	14	51,656,253 (GRCm39)	missense	probably damaging	0.97
R8448:Vmn2r88	UTSW	14	51,656,253 (GRCm39)	missense	probably damaging	0.97
R8483:Vmn2r88	UTSW	14	51,650,530 (GRCm39)	missense	possibly damaging	0.48
R8783:Vmn2r88	UTSW	14	51,651,523 (GRCm39)	missense
R8859:Vmn2r88	UTSW	14	51,656,263 (GRCm39)	missense	probably damaging	0.97
R8916:Vmn2r88	UTSW	14	51,648,593 (GRCm39)	missense
R8936:Vmn2r88	UTSW	14	51,655,983 (GRCm39)	missense	possibly damaging	0.88
R9004:Vmn2r88	UTSW	14	51,650,624 (GRCm39)	missense
R9038:Vmn2r88	UTSW	14	51,651,490 (GRCm39)	missense
R9063:Vmn2r88	UTSW	14	51,648,329 (GRCm39)	start gained	probably benign
R9311:Vmn2r88	UTSW	14	51,650,503 (GRCm39)	missense	probably benign	0.00
R9382:Vmn2r88	UTSW	14	51,656,197 (GRCm39)	missense
R9483:Vmn2r88	UTSW	14	51,648,641 (GRCm39)	missense
R9602:Vmn2r88	UTSW	14	51,651,189 (GRCm39)	missense
V5622:Vmn2r88	UTSW	14	51,650,584 (GRCm39)	missense	probably benign
X0024:Vmn2r88	UTSW	14	51,651,289 (GRCm39)	missense	possibly damaging	0.79
X0025:Vmn2r88	UTSW	14	51,654,259 (GRCm39)	missense	possibly damaging	0.59
Z1177:Vmn2r88	UTSW	14	51,655,644 (GRCm39)	missense
Z1177:Vmn2r88	UTSW	14	51,655,503 (GRCm39)	frame shift	probably null
Z1190:Vmn2r88	UTSW	14	51,650,658 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ACCCAACATAACTTTGATGTTCTCC -3'
(R):5'- CTAGAACACAGTTTTACCTGTTTGC -3'

Sequencing Primer
(F):5'- CCATCATTGGTGGAAACTGTC -3'
(R):5'- CCTGTTTGCAGTGTACATCTACGAAG -3'

Posted On

2015-07-06