Mutagenetix > Incidental Mutation

Incidental Mutation 'R4397:Dnajc15'

325560

Institutional Source

Beutler Lab

Gene Symbol

Dnajc15

Ensembl Gene

ENSMUSG00000022013

Gene Name

DnaJ heat shock protein family (Hsp40) member C15

Synonyms

Dnajd1, 1110003P16Rik

MMRRC Submission

041685-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4397 (G1)

Quality Score

181

Status

Validated

Chromosome

Chromosomal Location

78063657-78112357 bp(-) (GRCm39)

Type of Mutation

splice site (4 bp from exon)

DNA Base Change (assembly)

T to C at 78112234 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000154390 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022590] [ENSMUST00000226459]

AlphaFold

Q78YY6

Predicted Effect

probably benign
Transcript: ENSMUST00000022590

SMART Domains

Protein: ENSMUSP00000022590
Gene: ENSMUSG00000022013

Domain	Start	End	E-Value	Type
low complexity region	34	53	N/A	INTRINSIC
DnaJ	93	147	6.8e-7	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000226459

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

96% (55/57)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased mitochondrial activity that results in rapid metabolism in fasted mice or mice fed a high fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg4	T	G	X: 55,977,703 (GRCm39)	L2193V	probably damaging	Het
Agap2	G	A	10: 126,926,352 (GRCm39)	A866T	unknown	Het
Aig1	A	C	10: 13,528,726 (GRCm39)	S237A	probably benign	Het
Baz1b	C	T	5: 135,273,300 (GRCm39)	R1475W	probably damaging	Het
Bmp1	C	T	14: 70,727,982 (GRCm39)		probably null	Het
Crybg3	A	G	16: 59,380,458 (GRCm39)		probably benign	Het
Fam135b	T	A	15: 71,320,525 (GRCm39)	H1334L	probably benign	Het
Fancg	A	T	4: 43,008,897 (GRCm39)	H113Q	probably benign	Het
Gfap	G	A	11: 102,787,810 (GRCm39)	A45V	probably benign	Het
Gjd3	A	T	11: 98,873,247 (GRCm39)	L199Q	probably damaging	Het
Gvin-ps3	A	G	7: 105,682,130 (GRCm39)		noncoding transcript	Het
H13	A	G	2: 152,519,472 (GRCm39)	D65G	probably damaging	Het
Hcls1	T	C	16: 36,757,662 (GRCm39)	V5A	possibly damaging	Het
Hjurp	GT	GTT	1: 88,194,246 (GRCm39)		probably null	Het
Homer3	G	A	8: 70,742,793 (GRCm39)		probably null	Het
Iqgap3	T	C	3: 88,011,665 (GRCm39)	Y817H	probably damaging	Het
Iqsec2	C	T	X: 150,992,049 (GRCm39)	T562I	probably damaging	Het
Klb	A	G	5: 65,537,382 (GRCm39)	Y904C	probably damaging	Het
Kremen1	A	T	11: 5,149,610 (GRCm39)	S354T	probably benign	Het
Lamc3	A	G	2: 31,821,964 (GRCm39)	E1304G	probably benign	Het
Lrp4	T	C	2: 91,342,015 (GRCm39)	V1876A	probably benign	Het
Magi3	T	C	3: 104,127,030 (GRCm39)	T85A	probably damaging	Het
Map3k12	C	T	15: 102,409,694 (GRCm39)	A694T	probably benign	Het
Mex3b	T	C	7: 82,519,031 (GRCm39)	S449P	possibly damaging	Het
Naip6	C	T	13: 100,437,108 (GRCm39)	A472T	probably benign	Het
Nars2	T	C	7: 96,622,771 (GRCm39)		probably null	Het
Nlrp1a	T	G	11: 70,988,030 (GRCm39)	M1046L	probably benign	Het
Nphs1	T	G	7: 30,181,390 (GRCm39)		probably null	Het
Nup133	G	A	8: 124,671,040 (GRCm39)	T119M	probably benign	Het
Or6c201	G	T	10: 128,969,450 (GRCm39)	N62K	possibly damaging	Het
Pcdhga9	A	T	18: 37,871,694 (GRCm39)	I508F	probably damaging	Het
Phactr3	A	G	2: 177,817,199 (GRCm39)		probably benign	Het
Plcb3	T	C	19: 6,943,193 (GRCm39)	K155E	probably damaging	Het
Plxna2	C	T	1: 194,431,625 (GRCm39)	S538F	probably damaging	Het
Prss38	T	C	11: 59,263,854 (GRCm39)	Y286C	probably damaging	Het
Psg16	T	C	7: 16,824,623 (GRCm39)	S45P	possibly damaging	Het
Ptpn21	G	T	12: 98,654,507 (GRCm39)	P820Q	probably damaging	Het
Ptpn21	A	G	12: 98,681,319 (GRCm39)	V105A	probably damaging	Het
Rnf7	A	G	9: 96,360,463 (GRCm39)	M58T	probably benign	Het
Slc25a11	G	A	11: 70,535,677 (GRCm39)	A287V	probably benign	Het
Slit2	G	T	5: 48,377,423 (GRCm39)		probably null	Het
Suco	A	G	1: 161,672,421 (GRCm39)	Y460H	probably damaging	Het
Tnxb	C	T	17: 34,897,636 (GRCm39)	Q804*	probably null	Het
Trpv6	A	G	6: 41,602,172 (GRCm39)	I379T	possibly damaging	Het
Ugt1a1	CAGAGAGAGAGAGA	CAGAGAGAGAGA	1: 88,139,706 (GRCm39)		probably benign	Het
Virma	A	G	4: 11,513,901 (GRCm39)	E585G	possibly damaging	Het
Vmn2r79	A	G	7: 86,651,099 (GRCm39)	H166R	possibly damaging	Het
Vmn2r88	A	T	14: 51,655,435 (GRCm39)	D549V	probably damaging	Het

Other mutations in Dnajc15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02607:Dnajc15	APN	14	78,077,656 (GRCm39)	missense	probably damaging	1.00
R0321:Dnajc15	UTSW	14	78,112,273 (GRCm39)	missense	possibly damaging	0.90
R1574:Dnajc15	UTSW	14	78,063,854 (GRCm39)	missense	probably benign	0.00
R1574:Dnajc15	UTSW	14	78,063,854 (GRCm39)	missense	probably benign	0.00
R3774:Dnajc15	UTSW	14	78,094,377 (GRCm39)	critical splice donor site	probably null
R4747:Dnajc15	UTSW	14	78,081,896 (GRCm39)	missense	probably benign	0.13
R5704:Dnajc15	UTSW	14	78,063,898 (GRCm39)	missense	probably damaging	1.00
R7848:Dnajc15	UTSW	14	78,077,643 (GRCm39)	missense	probably damaging	0.99
R8179:Dnajc15	UTSW	14	78,090,393 (GRCm39)	missense
R8882:Dnajc15	UTSW	14	78,094,411 (GRCm39)	missense	probably damaging	1.00
R9260:Dnajc15	UTSW	14	78,081,839 (GRCm39)	missense	possibly damaging	0.86

Predicted Primers

PCR Primer
(F):5'- GCGCCCTGGTTCTTAACTAC -3'
(R):5'- ACCGGTCCGCACTAATTTG -3'

Sequencing Primer
(F):5'- GGTTCTTAACTACTTTTCCCCAAGGG -3'
(R):5'- GCACTAATTTGTCCCCGCGG -3'

Posted On

2015-07-06