Mutagenetix > Incidental Mutation

Incidental Mutation 'R4367:Vmn2r25'

325802

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r25

Ensembl Gene

ENSMUSG00000094672

Gene Name

vomeronasal 2, receptor 25

Synonyms

EG545874

MMRRC Submission

041673-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R4367 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

123799773-123830149 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 123805496 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glycine at position 454 (R454G)

Ref Sequence

ENSEMBL: ENSMUSP00000124342 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000162046]

AlphaFold

W4VSP2

Predicted Effect

probably damaging
Transcript: ENSMUST00000162046
AA Change: R454G

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000124342
Gene: ENSMUSG00000094672
AA Change: R454G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	473	6e-31	PFAM
Pfam:NCD3G	519	572	5.8e-25	PFAM
Pfam:7tm_3	603	840	4.8e-55	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.0%
20x: 94.4%

Validation Efficiency

98% (54/55)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adrb2	T	C	18: 62,312,127 (GRCm39)	I233V	probably damaging	Het
Alox5	T	A	6: 116,437,924 (GRCm39)	Y21F	possibly damaging	Het
Ank2	T	C	3: 126,739,798 (GRCm39)	T1942A	probably benign	Het
Aoc1l1	T	C	6: 48,953,064 (GRCm39)	S330P	probably damaging	Het
B4galt3	C	A	1: 171,101,613 (GRCm39)	H196N	probably damaging	Het
Bckdk	C	T	7: 127,505,591 (GRCm39)	A238V	probably benign	Het
Casp1	A	G	9: 5,299,333 (GRCm39)	T21A	probably benign	Het
Ccdc39	T	C	3: 33,880,671 (GRCm39)	H432R	probably benign	Het
Cttnbp2	A	C	6: 18,405,248 (GRCm39)	C574G	probably damaging	Het
Cyp1a1	T	C	9: 57,607,432 (GRCm39)	V20A	probably benign	Het
Dhx38	C	T	8: 110,279,763 (GRCm39)	V976I	probably damaging	Het
Dnah6	A	G	6: 73,126,467 (GRCm39)	S1287P	possibly damaging	Het
Dnttip2	T	C	3: 122,070,146 (GRCm39)	S454P	probably damaging	Het
Drp2	A	T	X: 133,335,884 (GRCm39)		probably benign	Het
Flcn	C	T	11: 59,694,610 (GRCm39)	V121I	possibly damaging	Het
Fmo1	G	C	1: 162,661,217 (GRCm39)	Y355*	probably null	Het
Git2	T	A	5: 114,902,727 (GRCm39)	H138L	probably damaging	Het
Gpr162	G	A	6: 124,838,658 (GRCm39)		probably benign	Het
Kcnd3	C	T	3: 105,566,082 (GRCm39)	A421V	probably damaging	Het
Kcnt1	T	C	2: 25,797,638 (GRCm39)	I881T	probably damaging	Het
Lama3	T	A	18: 12,646,747 (GRCm39)	C1754S	probably damaging	Het
Mpp3	A	T	11: 101,914,246 (GRCm39)	D116E	probably benign	Het
Myh11	T	C	16: 14,036,747 (GRCm39)	D985G	probably damaging	Het
Necap1	T	C	6: 122,864,337 (GRCm39)	V273A	probably damaging	Het
Nlrc5	T	C	8: 95,203,192 (GRCm39)	S431P	probably damaging	Het
Nutm2	A	T	13: 50,623,920 (GRCm39)	T206S	probably benign	Het
Or2d3	GAACAACAACAA	GAACAACAA	7: 106,490,567 (GRCm39)		probably benign	Het
Or5p79	C	T	7: 108,221,096 (GRCm39)	L26F	probably benign	Het
Or8g19	G	A	9: 39,055,725 (GRCm39)	A110T	probably damaging	Het
Phactr2	A	G	10: 13,129,564 (GRCm39)	S235P	probably damaging	Het
Podnl1	G	A	8: 84,853,897 (GRCm39)	R89H	probably benign	Het
Prpf38b	T	C	3: 108,818,487 (GRCm39)	Y91C	probably damaging	Het
Radil	C	T	5: 142,480,560 (GRCm39)	A632T	probably benign	Het
Rpap2	G	A	5: 107,749,661 (GRCm39)	V62I	possibly damaging	Het
Sdf2	C	T	11: 78,141,863 (GRCm39)	T66I	probably damaging	Het
Specc1	T	C	11: 62,009,356 (GRCm39)	S371P	probably damaging	Het
Suco	T	C	1: 161,674,799 (GRCm39)	E416G	probably damaging	Het
Sys1	T	C	2: 164,303,315 (GRCm39)	W10R	probably damaging	Het
Tars3	C	T	7: 65,332,567 (GRCm39)	T556M	probably damaging	Het
Tcirg1	C	T	19: 3,949,069 (GRCm39)	D407N	probably damaging	Het
Tefm	G	T	11: 80,031,156 (GRCm39)	L27I	probably benign	Het
Tenm2	A	G	11: 35,918,225 (GRCm39)	I1845T	probably benign	Het
Tfam	A	T	10: 71,069,233 (GRCm39)	I119N	probably damaging	Het
Tle1	ACAGGTTTCTTCAGGTTTCTT	ACAGGTTTCTT	4: 72,036,400 (GRCm39)		probably benign	Het
Trpm6	T	C	19: 18,804,889 (GRCm39)	I947T	probably damaging	Het
Ubqlnl	C	T	7: 103,798,925 (GRCm39)	V191M	probably benign	Het
Usp54	T	C	14: 20,611,202 (GRCm39)	T1205A	probably benign	Het
Xylb	T	C	9: 119,217,781 (GRCm39)	V477A	probably benign	Het

Other mutations in Vmn2r25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Vmn2r25	APN	6	123,830,130 (GRCm39)	missense	probably benign	0.25
IGL01781:Vmn2r25	APN	6	123,816,324 (GRCm39)	missense	possibly damaging	0.48
IGL01843:Vmn2r25	APN	6	123,829,962 (GRCm39)	missense	possibly damaging	0.67
IGL02023:Vmn2r25	APN	6	123,816,388 (GRCm39)	missense	probably damaging	0.96
IGL02502:Vmn2r25	APN	6	123,816,392 (GRCm39)	missense	probably damaging	0.96
IGL02709:Vmn2r25	APN	6	123,816,723 (GRCm39)	missense	possibly damaging	0.50
IGL03053:Vmn2r25	APN	6	123,800,077 (GRCm39)	missense	probably damaging	1.00
PIT4468001:Vmn2r25	UTSW	6	123,816,557 (GRCm39)	missense	probably benign	0.00
PIT4812001:Vmn2r25	UTSW	6	123,800,447 (GRCm39)	missense	probably damaging	1.00
R0054:Vmn2r25	UTSW	6	123,829,984 (GRCm39)	missense	probably benign	0.00
R0312:Vmn2r25	UTSW	6	123,805,539 (GRCm39)	splice site	probably benign
R0366:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R0390:Vmn2r25	UTSW	6	123,800,140 (GRCm39)	missense	probably damaging	1.00
R0466:Vmn2r25	UTSW	6	123,829,008 (GRCm39)	missense	probably benign	0.16
R0541:Vmn2r25	UTSW	6	123,816,786 (GRCm39)	missense	probably damaging	0.97
R0612:Vmn2r25	UTSW	6	123,816,481 (GRCm39)	missense	probably damaging	1.00
R0865:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R1219:Vmn2r25	UTSW	6	123,816,282 (GRCm39)	missense	probably benign	0.00
R1240:Vmn2r25	UTSW	6	123,828,864 (GRCm39)	missense	probably damaging	0.98
R1701:Vmn2r25	UTSW	6	123,828,754 (GRCm39)	splice site	probably null
R1780:Vmn2r25	UTSW	6	123,805,424 (GRCm39)	missense	probably damaging	1.00
R1809:Vmn2r25	UTSW	6	123,802,337 (GRCm39)	missense	probably benign	0.00
R1833:Vmn2r25	UTSW	6	123,816,643 (GRCm39)	missense	probably benign	0.01
R1964:Vmn2r25	UTSW	6	123,800,254 (GRCm39)	missense	possibly damaging	0.94
R2154:Vmn2r25	UTSW	6	123,816,805 (GRCm39)	missense	probably benign	0.01
R2164:Vmn2r25	UTSW	6	123,816,518 (GRCm39)	missense	possibly damaging	0.96
R3799:Vmn2r25	UTSW	6	123,830,143 (GRCm39)	missense	probably benign	0.12
R3836:Vmn2r25	UTSW	6	123,830,044 (GRCm39)	missense	probably damaging	1.00
R3946:Vmn2r25	UTSW	6	123,817,057 (GRCm39)	missense	probably damaging	0.97
R4282:Vmn2r25	UTSW	6	123,800,606 (GRCm39)	missense	probably damaging	1.00
R4438:Vmn2r25	UTSW	6	123,816,756 (GRCm39)	missense	probably benign	0.03
R4488:Vmn2r25	UTSW	6	123,799,819 (GRCm39)	missense	probably damaging	1.00
R4580:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.46
R4631:Vmn2r25	UTSW	6	123,829,962 (GRCm39)	missense	possibly damaging	0.94
R4765:Vmn2r25	UTSW	6	123,800,182 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r25	UTSW	6	123,805,406 (GRCm39)	missense	probably benign
R5207:Vmn2r25	UTSW	6	123,817,062 (GRCm39)	missense	probably damaging	1.00
R5254:Vmn2r25	UTSW	6	123,802,277 (GRCm39)	missense	probably damaging	1.00
R5444:Vmn2r25	UTSW	6	123,805,451 (GRCm39)	missense	probably benign	0.00
R5586:Vmn2r25	UTSW	6	123,802,255 (GRCm39)	missense	probably damaging	1.00
R5607:Vmn2r25	UTSW	6	123,805,318 (GRCm39)	missense	possibly damaging	0.49
R5985:Vmn2r25	UTSW	6	123,800,587 (GRCm39)	missense	probably benign
R6046:Vmn2r25	UTSW	6	123,799,876 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r25	UTSW	6	123,799,900 (GRCm39)	missense	possibly damaging	0.69
R6569:Vmn2r25	UTSW	6	123,828,941 (GRCm39)	missense	probably benign	0.01
R6826:Vmn2r25	UTSW	6	123,800,071 (GRCm39)	missense	probably damaging	1.00
R7054:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R7120:Vmn2r25	UTSW	6	123,805,394 (GRCm39)	missense	possibly damaging	0.51
R7177:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7287:Vmn2r25	UTSW	6	123,829,040 (GRCm39)	missense	possibly damaging	0.49
R7397:Vmn2r25	UTSW	6	123,800,498 (GRCm39)	missense	probably damaging	0.96
R7486:Vmn2r25	UTSW	6	123,800,101 (GRCm39)	missense	probably damaging	1.00
R7699:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7700:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7759:Vmn2r25	UTSW	6	123,800,339 (GRCm39)	missense	probably damaging	0.99
R7802:Vmn2r25	UTSW	6	123,828,791 (GRCm39)	missense	possibly damaging	0.88
R7850:Vmn2r25	UTSW	6	123,805,431 (GRCm39)	missense	probably damaging	1.00
R8064:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R8170:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R8340:Vmn2r25	UTSW	6	123,829,972 (GRCm39)	missense	probably benign	0.01
R8346:Vmn2r25	UTSW	6	123,802,350 (GRCm39)	missense	probably benign	0.00
R8395:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.81
R8889:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R9094:Vmn2r25	UTSW	6	123,805,391 (GRCm39)	missense	probably benign	0.44
R9204:Vmn2r25	UTSW	6	123,830,092 (GRCm39)	missense	probably benign	0.00
R9253:Vmn2r25	UTSW	6	123,816,960 (GRCm39)	missense	probably damaging	0.98
R9392:Vmn2r25	UTSW	6	123,816,937 (GRCm39)	missense	probably benign
R9520:Vmn2r25	UTSW	6	123,830,066 (GRCm39)	nonsense	probably null
R9525:Vmn2r25	UTSW	6	123,800,164 (GRCm39)	missense	probably damaging	1.00
R9622:Vmn2r25	UTSW	6	123,816,579 (GRCm39)	missense	probably damaging	0.99
X0020:Vmn2r25	UTSW	6	123,816,359 (GRCm39)	missense	possibly damaging	0.95
Z1176:Vmn2r25	UTSW	6	123,799,856 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCTGATTGTGGTGCTCAC -3'
(R):5'- TCTACATATAGACCTACTGCTAGCATG -3'

Sequencing Primer
(F):5'- TGCTCACCCCAAGTTATTATTTTATC -3'
(R):5'- AGACCTACTGCTAGCATGTATTAGCC -3'

Posted On

2015-07-06