Incidental Mutation 'R4401:Olfr447'
ID326628
Institutional Source Beutler Lab
Gene Symbol Olfr447
Ensembl Gene ENSMUSG00000045708
Gene Nameolfactory receptor 447
SynonymsMOR261-1, GA_x6K02T2P3E9-4647978-4647046
MMRRC Submission 041132-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.238) question?
Stock #R4401 (G1)
Quality Score225
Status Not validated
Chromosome6
Chromosomal Location42908878-42914273 bp(+) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) G to T at 42912326 bp
ZygosityHeterozygous
Amino Acid Change Glutamic Acid to Stop codon at position 268 (E268*)
Ref Sequence ENSEMBL: ENSMUSP00000149325 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000055763] [ENSMUST00000216408]
Predicted Effect probably null
Transcript: ENSMUST00000055763
AA Change: E268*
SMART Domains Protein: ENSMUSP00000052312
Gene: ENSMUSG00000045708
AA Change: E268*

DomainStartEndE-ValueType
Pfam:7tm_4 30 307 1.8e-60 PFAM
Pfam:7tm_1 40 289 6.3e-27 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000216408
AA Change: E268*
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.1%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 32 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb1a A G 5: 8,702,390 I454V possibly damaging Het
Acvr2a A G 2: 48,899,702 T486A probably benign Het
Ap3b1 C A 13: 94,418,099 L248I probably damaging Het
Atad2b T A 12: 4,940,145 C157S probably damaging Het
C530008M17Rik G A 5: 76,848,916 V74I probably damaging Het
Cage1 T A 13: 38,023,102 I256F probably damaging Het
Cct2 A G 10: 117,057,809 I287T possibly damaging Het
Cntn4 A G 6: 106,489,664 T176A possibly damaging Het
Cyp2c54 T C 19: 40,072,171 N122S probably benign Het
Cytip T C 2: 58,133,935 D291G probably benign Het
Eef1d C T 15: 75,902,920 V213I probably benign Het
Fnbp4 A G 2: 90,746,758 T145A possibly damaging Het
Fras1 C A 5: 96,642,620 T951K probably damaging Het
Gm11937 C T 11: 99,610,075 V39M probably damaging Het
Gm6712 C T 17: 17,318,104 noncoding transcript Het
Hira A G 16: 18,925,720 I352V probably damaging Het
Homer3 G A 8: 70,290,143 probably null Het
Lpcat2 T C 8: 92,873,055 V217A possibly damaging Het
Mroh2a G T 1: 88,254,935 R1195L possibly damaging Het
Olfr1277 A G 2: 111,269,833 F178S probably damaging Het
Olfr561 C T 7: 102,774,799 R92* probably null Het
Pla2g1b T A 5: 115,470,888 Y47* probably null Het
Pqlc2 T C 4: 139,306,543 I22V probably benign Het
Rfng A T 11: 120,782,480 V245E possibly damaging Het
Rpf2 A G 10: 40,236,128 V104A possibly damaging Het
Rps6kc1 G T 1: 190,799,958 H616N probably benign Het
Slc9b2 T C 3: 135,336,544 V528A probably benign Het
Srgap1 T C 10: 121,804,921 probably null Het
Trrap A G 5: 144,843,318 T3240A possibly damaging Het
Vmn2r68 TCC TC 7: 85,221,550 probably null Het
Zfhx4 C A 3: 5,403,345 Y2854* probably null Het
Zfp986 C T 4: 145,898,943 R58C probably benign Het
Other mutations in Olfr447
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02302:Olfr447 APN 6 42912338 missense probably damaging 1.00
IGL02604:Olfr447 APN 6 42912058 nonsense probably null
IGL02894:Olfr447 APN 6 42911517 splice site probably benign
R0122:Olfr447 UTSW 6 42911955 missense probably benign 0.00
R0172:Olfr447 UTSW 6 42911979 missense probably benign 0.00
R0371:Olfr447 UTSW 6 42911938 missense probably benign 0.14
R1709:Olfr447 UTSW 6 42912144 missense possibly damaging 0.94
R2431:Olfr447 UTSW 6 42912012 missense probably damaging 0.99
R3809:Olfr447 UTSW 6 42912337 missense probably damaging 1.00
R4032:Olfr447 UTSW 6 42911625 missense probably benign 0.08
R4242:Olfr447 UTSW 6 42911546 missense possibly damaging 0.77
R4369:Olfr447 UTSW 6 42912277 nonsense probably null
R4565:Olfr447 UTSW 6 42911538 missense probably benign
R5646:Olfr447 UTSW 6 42911523 unclassified probably null
R5968:Olfr447 UTSW 6 42911546 missense probably benign
R6804:Olfr447 UTSW 6 42911918 missense probably benign
R6925:Olfr447 UTSW 6 42911857 nonsense probably null
Predicted Primers PCR Primer
(F):5'- GTGCTTGTCGGACCATTTTC -3'
(R):5'- GATGAACAAGTTACATGTCCTGGTC -3'

Sequencing Primer
(F):5'- GTCGGACCATTTTCTTCAATTGTAG -3'
(R):5'- AGTGTCCTCTTCAGAGCA -3'
Posted On2015-07-07