Mutagenetix > Incidental Mutation

Incidental Mutation 'R4405:Vmn1r20'

327610

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r20

Ensembl Gene

ENSMUSG00000115253

Gene Name

vomeronasal 1 receptor 20

Synonyms

Gm5569

MMRRC Submission

041134-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.416) question?

Stock #

R4405 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

57408676-57409587 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 57409042 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 123 (M123V)

Ref Sequence

ENSEMBL: ENSMUSP00000153815 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176085] [ENSMUST00000226808] [ENSMUST00000227909]

AlphaFold

K7N778

Predicted Effect

probably benign
Transcript: ENSMUST00000176085
AA Change: M123V

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000134973
Gene: ENSMUSG00000093679
AA Change: M123V

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	2.3e-51	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000226808
AA Change: M123V

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

Predicted Effect

probably benign
Transcript: ENSMUST00000227909
AA Change: M123V

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg3	C	T	8: 95,763,536 (GRCm39)	A279V	probably benign	Het
Aggf1	G	A	13: 95,508,102 (GRCm39)	P88L	probably benign	Het
Asb18	T	C	1: 89,896,411 (GRCm39)	T210A	probably benign	Het
Cd8b1	C	T	6: 71,303,006 (GRCm39)	S27L	possibly damaging	Het
Cfap43	C	T	19: 47,728,236 (GRCm39)	S1507N	possibly damaging	Het
Col12a1	A	T	9: 79,547,247 (GRCm39)		probably null	Het
Col19a1	G	A	1: 24,573,190 (GRCm39)	T194M	unknown	Het
Cpb1	T	A	3: 20,317,733 (GRCm39)	T237S	probably benign	Het
Cubn	G	A	2: 13,470,841 (GRCm39)	T517M	probably damaging	Het
Dnah6	T	C	6: 73,106,274 (GRCm39)	N1769S	probably benign	Het
Dnm1	A	G	2: 32,225,984 (GRCm39)	I365T	probably damaging	Het
E2f5	T	A	3: 14,668,823 (GRCm39)	D238E	probably benign	Het
Etv6	A	G	6: 134,210,497 (GRCm39)	E115G	probably damaging	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Gapvd1	C	T	2: 34,618,747 (GRCm39)	V209I	probably damaging	Het
Gbp8	G	A	5: 105,198,928 (GRCm39)		probably benign	Het
Gprc6a	T	C	10: 51,504,639 (GRCm39)	I68M	probably benign	Het
Grb14	T	C	2: 64,783,966 (GRCm39)	N54D	probably damaging	Het
Gucy2g	A	T	19: 55,226,269 (GRCm39)	F216I	probably benign	Het
Herc2	T	A	7: 55,820,225 (GRCm39)	L2849Q	probably damaging	Het
Hsd17b4	G	T	18: 50,261,381 (GRCm39)		probably benign	Het
Igkv9-129	T	A	6: 67,816,784 (GRCm39)	M3K	probably damaging	Het
Iqcd	A	T	5: 120,740,485 (GRCm39)	E272V	probably damaging	Het
Itih2	A	T	2: 10,111,548 (GRCm39)	Y463*	probably null	Het
Kcna10	G	A	3: 107,102,781 (GRCm39)	E471K	possibly damaging	Het
Klf3	A	T	5: 64,986,347 (GRCm39)	E294V	possibly damaging	Het
Lancl1	A	T	1: 67,060,015 (GRCm39)		probably null	Het
Ldaf1	T	G	7: 119,714,714 (GRCm39)	F68V	probably damaging	Het
Lrig3	T	C	10: 125,846,877 (GRCm39)	F882L	probably benign	Het
Man2c1	A	G	9: 57,046,367 (GRCm39)	N474S	probably damaging	Het
Map4k3	A	G	17: 80,922,444 (GRCm39)		probably null	Het
Mast1	C	A	8: 85,647,520 (GRCm39)	W572C	probably damaging	Het
Mbd4	T	A	6: 115,826,076 (GRCm39)	Q305L	possibly damaging	Het
Mbl2	T	A	19: 30,216,916 (GRCm39)	S243T	probably benign	Het
Mcph1	C	T	8: 18,682,557 (GRCm39)	P565S	probably benign	Het
Mfsd1	G	C	3: 67,507,943 (GRCm39)	V435L	probably benign	Het
Mycbp2	C	T	14: 103,360,881 (GRCm39)	G1183D	probably damaging	Het
Myof	A	G	19: 37,911,426 (GRCm39)	S1502P	probably damaging	Het
Noa1	A	T	5: 77,454,219 (GRCm39)	S474T	probably benign	Het
Nrde2	A	G	12: 100,096,843 (GRCm39)	Y986H	probably benign	Het
Oca2	G	T	7: 56,064,182 (GRCm39)	V726F	possibly damaging	Het
Or2ag13	T	A	7: 106,472,580 (GRCm39)	S291C	probably damaging	Het
Or7a41	A	T	10: 78,871,244 (GRCm39)	M205L	probably benign	Het
Pip5k1a	A	G	3: 94,975,370 (GRCm39)		probably null	Het
Plekhn1	A	T	4: 156,309,730 (GRCm39)		probably null	Het
Sec61a2	T	C	2: 5,887,670 (GRCm39)	D104G	probably benign	Het
Skic3	A	T	13: 76,303,784 (GRCm39)	Y1234F	probably damaging	Het
Slc25a36	T	C	9: 96,967,171 (GRCm39)	T131A	probably benign	Het
Slc5a11	A	T	7: 122,857,700 (GRCm39)	D263V	probably damaging	Het
Sorbs1	T	C	19: 40,384,189 (GRCm39)	I35V	probably benign	Het
Speer3	C	G	5: 13,846,394 (GRCm39)	A238G	possibly damaging	Het
Tbc1d1	T	C	5: 64,331,013 (GRCm39)	V64A	possibly damaging	Het
Ubqlnl	C	T	7: 103,798,925 (GRCm39)	V191M	probably benign	Het
Zwint	T	C	10: 72,492,095 (GRCm39)	L59P	probably damaging	Het

Other mutations in Vmn1r20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01761:Vmn1r20	APN	6	57,408,725 (GRCm39)	missense	probably damaging	1.00
IGL02622:Vmn1r20	APN	6	57,409,583 (GRCm39)	missense	probably damaging	0.99
IGL02636:Vmn1r20	APN	6	57,408,746 (GRCm39)	missense	probably benign
R0006:Vmn1r20	UTSW	6	57,409,290 (GRCm39)	missense	probably damaging	0.99
R0079:Vmn1r20	UTSW	6	57,408,777 (GRCm39)	missense	possibly damaging	0.70
R0200:Vmn1r20	UTSW	6	57,409,084 (GRCm39)	missense	probably damaging	0.96
R1675:Vmn1r20	UTSW	6	57,408,937 (GRCm39)	missense	probably benign	0.00
R1734:Vmn1r20	UTSW	6	57,409,285 (GRCm39)	missense	probably damaging	1.00
R1883:Vmn1r20	UTSW	6	57,409,306 (GRCm39)	missense	probably benign	0.14
R2049:Vmn1r20	UTSW	6	57,408,943 (GRCm39)	missense	probably damaging	1.00
R2307:Vmn1r20	UTSW	6	57,409,121 (GRCm39)	missense	probably benign	0.03
R2346:Vmn1r20	UTSW	6	57,408,931 (GRCm39)	missense	possibly damaging	0.83
R4946:Vmn1r20	UTSW	6	57,409,159 (GRCm39)	missense	probably damaging	0.98
R5259:Vmn1r20	UTSW	6	57,409,050 (GRCm39)	nonsense	probably null
R5321:Vmn1r20	UTSW	6	57,409,427 (GRCm39)	missense	probably benign	0.21
R5911:Vmn1r20	UTSW	6	57,408,774 (GRCm39)	missense	probably benign	0.16
R6042:Vmn1r20	UTSW	6	57,409,391 (GRCm39)	missense	possibly damaging	0.90
R6298:Vmn1r20	UTSW	6	57,409,112 (GRCm39)	missense	probably benign
R6314:Vmn1r20	UTSW	6	57,409,042 (GRCm39)	missense	probably benign	0.00
R6331:Vmn1r20	UTSW	6	57,408,655 (GRCm39)	splice site	probably null
R6482:Vmn1r20	UTSW	6	57,409,093 (GRCm39)	missense	probably benign	0.01
R9161:Vmn1r20	UTSW	6	57,408,759 (GRCm39)	missense	probably benign	0.00
R9165:Vmn1r20	UTSW	6	57,409,246 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCATTCACATAATGGTGTTCCTCAC -3'
(R):5'- ACATCTCTGGAGGTTGTCACTG -3'

Sequencing Primer
(F):5'- GCTTACAGACATATTTGAGTCACTG -3'
(R):5'- CTCTGGAGGTTGTCACTGTAAAAATG -3'

Posted On

2015-07-07