Incidental Mutation 'R4410:Spag7'
| ID |
327872 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Spag7
|
| Ensembl Gene |
ENSMUSG00000018287 |
| Gene Name |
sperm associated antigen 7 |
| Synonyms |
FSA-1, Fsa1l |
| MMRRC Submission |
041692-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R4410 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
11 |
| Chromosomal Location |
70554597-70560242 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 70555688 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Aspartic acid to Glycine
at position 83
(D83G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000018431
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000018431]
[ENSMUST00000036299]
[ENSMUST00000072841]
[ENSMUST00000100933]
[ENSMUST00000108544]
[ENSMUST00000108545]
[ENSMUST00000108548]
[ENSMUST00000129434]
[ENSMUST00000119120]
[ENSMUST00000157027]
[ENSMUST00000120261]
[ENSMUST00000170716]
[ENSMUST00000145823]
|
| AlphaFold |
Q7TNE3 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000018431
AA Change: D83G
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000018431
Gene: ENSMUSG00000018287
AA Change: D83G
| Domain | Start | End | E-Value | Type |
|---|
|
R3H
|
31 |
109 |
3.85e-21 |
SMART |
|
low complexity region
|
130 |
152 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000036299
|
| SMART Domains |
Protein: ENSMUSP00000043792
Gene: ENSMUSG00000040712
| Domain | Start | End | E-Value | Type |
|---|
|
CG-1
|
34 |
155 |
1.07e-83 |
SMART |
|
low complexity region
|
232 |
243 |
N/A |
INTRINSIC |
|
low complexity region
|
273 |
291 |
N/A |
INTRINSIC |
|
low complexity region
|
294 |
305 |
N/A |
INTRINSIC |
|
low complexity region
|
314 |
329 |
N/A |
INTRINSIC |
|
low complexity region
|
370 |
380 |
N/A |
INTRINSIC |
|
low complexity region
|
417 |
435 |
N/A |
INTRINSIC |
|
low complexity region
|
461 |
485 |
N/A |
INTRINSIC |
|
low complexity region
|
501 |
514 |
N/A |
INTRINSIC |
|
Pfam:TIG
|
541 |
621 |
6.2e-13 |
PFAM |
|
low complexity region
|
660 |
679 |
N/A |
INTRINSIC |
|
Blast:ANK
|
717 |
750 |
7e-12 |
BLAST |
|
SCOP:d1myo__
|
718 |
816 |
2e-15 |
SMART |
|
Blast:ANK
|
762 |
792 |
4e-11 |
BLAST |
|
low complexity region
|
829 |
839 |
N/A |
INTRINSIC |
|
low complexity region
|
844 |
853 |
N/A |
INTRINSIC |
|
low complexity region
|
861 |
882 |
N/A |
INTRINSIC |
|
IQ
|
1053 |
1075 |
2.59e2 |
SMART |
|
IQ
|
1076 |
1092 |
2.38e2 |
SMART |
|
IQ
|
1106 |
1128 |
5.42e0 |
SMART |
|
low complexity region
|
1140 |
1157 |
N/A |
INTRINSIC |
|
low complexity region
|
1180 |
1190 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000072841
|
| SMART Domains |
Protein: ENSMUSP00000072620
Gene: ENSMUSG00000060600
| Domain | Start | End | E-Value | Type |
|---|
|
Enolase_N
|
3 |
134 |
6.26e-91 |
SMART |
|
Enolase_C
|
142 |
431 |
8.8e-200 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000100933
|
| SMART Domains |
Protein: ENSMUSP00000098493
Gene: ENSMUSG00000040712
| Domain | Start | End | E-Value | Type |
|---|
|
CG-1
|
36 |
152 |
8.08e-88 |
SMART |
|
low complexity region
|
229 |
240 |
N/A |
INTRINSIC |
|
low complexity region
|
270 |
288 |
N/A |
INTRINSIC |
|
low complexity region
|
291 |
302 |
N/A |
INTRINSIC |
|
low complexity region
|
311 |
326 |
N/A |
INTRINSIC |
|
low complexity region
|
367 |
377 |
N/A |
INTRINSIC |
|
low complexity region
|
414 |
432 |
N/A |
INTRINSIC |
|
low complexity region
|
458 |
482 |
N/A |
INTRINSIC |
|
low complexity region
|
498 |
511 |
N/A |
INTRINSIC |
|
Pfam:TIG
|
538 |
618 |
1.2e-8 |
PFAM |
|
low complexity region
|
657 |
676 |
N/A |
INTRINSIC |
|
Blast:ANK
|
714 |
747 |
8e-12 |
BLAST |
|
SCOP:d1myo__
|
715 |
813 |
2e-15 |
SMART |
|
Blast:ANK
|
759 |
789 |
4e-11 |
BLAST |
|
low complexity region
|
826 |
836 |
N/A |
INTRINSIC |
|
low complexity region
|
841 |
850 |
N/A |
INTRINSIC |
|
low complexity region
|
858 |
879 |
N/A |
INTRINSIC |
|
IQ
|
1050 |
1072 |
2.59e2 |
SMART |
|
IQ
|
1073 |
1095 |
1.18e1 |
SMART |
|
IQ
|
1096 |
1118 |
5.42e0 |
SMART |
|
low complexity region
|
1130 |
1147 |
N/A |
INTRINSIC |
|
low complexity region
|
1170 |
1180 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108544
|
| SMART Domains |
Protein: ENSMUSP00000104184
Gene: ENSMUSG00000040712
| Domain | Start | End | E-Value | Type |
|---|
|
CG-1
|
34 |
150 |
8.08e-88 |
SMART |
|
low complexity region
|
227 |
238 |
N/A |
INTRINSIC |
|
low complexity region
|
268 |
286 |
N/A |
INTRINSIC |
|
low complexity region
|
289 |
300 |
N/A |
INTRINSIC |
|
low complexity region
|
309 |
324 |
N/A |
INTRINSIC |
|
low complexity region
|
365 |
375 |
N/A |
INTRINSIC |
|
low complexity region
|
412 |
430 |
N/A |
INTRINSIC |
|
low complexity region
|
456 |
480 |
N/A |
INTRINSIC |
|
low complexity region
|
496 |
509 |
N/A |
INTRINSIC |
|
Pfam:TIG
|
536 |
616 |
1.2e-8 |
PFAM |
|
low complexity region
|
655 |
674 |
N/A |
INTRINSIC |
|
Blast:ANK
|
712 |
745 |
7e-12 |
BLAST |
|
SCOP:d1myo__
|
713 |
811 |
2e-15 |
SMART |
|
Blast:ANK
|
757 |
787 |
4e-11 |
BLAST |
|
low complexity region
|
824 |
834 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
848 |
N/A |
INTRINSIC |
|
low complexity region
|
856 |
877 |
N/A |
INTRINSIC |
|
IQ
|
1048 |
1070 |
2.59e2 |
SMART |
|
IQ
|
1071 |
1087 |
2.38e2 |
SMART |
|
IQ
|
1101 |
1123 |
5.42e0 |
SMART |
|
low complexity region
|
1135 |
1152 |
N/A |
INTRINSIC |
|
low complexity region
|
1175 |
1185 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108545
|
| SMART Domains |
Protein: ENSMUSP00000104185
Gene: ENSMUSG00000040712
| Domain | Start | End | E-Value | Type |
|---|
|
CG-1
|
34 |
126 |
3.23e-55 |
SMART |
|
low complexity region
|
203 |
214 |
N/A |
INTRINSIC |
|
low complexity region
|
244 |
262 |
N/A |
INTRINSIC |
|
low complexity region
|
265 |
276 |
N/A |
INTRINSIC |
|
low complexity region
|
285 |
300 |
N/A |
INTRINSIC |
|
low complexity region
|
341 |
351 |
N/A |
INTRINSIC |
|
low complexity region
|
388 |
406 |
N/A |
INTRINSIC |
|
low complexity region
|
432 |
456 |
N/A |
INTRINSIC |
|
low complexity region
|
472 |
485 |
N/A |
INTRINSIC |
|
Pfam:TIG
|
512 |
592 |
1.1e-8 |
PFAM |
|
low complexity region
|
631 |
650 |
N/A |
INTRINSIC |
|
Blast:ANK
|
688 |
721 |
7e-12 |
BLAST |
|
SCOP:d1myo__
|
689 |
787 |
2e-15 |
SMART |
|
Blast:ANK
|
733 |
763 |
5e-13 |
BLAST |
|
low complexity region
|
800 |
810 |
N/A |
INTRINSIC |
|
low complexity region
|
815 |
824 |
N/A |
INTRINSIC |
|
low complexity region
|
832 |
853 |
N/A |
INTRINSIC |
|
IQ
|
1024 |
1046 |
2.59e2 |
SMART |
|
IQ
|
1047 |
1069 |
1.18e1 |
SMART |
|
IQ
|
1070 |
1092 |
5.42e0 |
SMART |
|
low complexity region
|
1104 |
1121 |
N/A |
INTRINSIC |
|
low complexity region
|
1144 |
1154 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108548
|
| SMART Domains |
Protein: ENSMUSP00000104188
Gene: ENSMUSG00000060600
| Domain | Start | End | E-Value | Type |
|---|
|
Enolase_N
|
3 |
134 |
6.26e-91 |
SMART |
|
Enolase_C
|
142 |
431 |
8.8e-200 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000129434
AA Change: D73G
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000115098
Gene: ENSMUSG00000018287
AA Change: D73G
| Domain | Start | End | E-Value | Type |
|---|
|
R3H
|
22 |
99 |
3.06e-15 |
SMART |
|
low complexity region
|
120 |
142 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000124691
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000137691
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000125687
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000179738
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000119120
|
| SMART Domains |
Protein: ENSMUSP00000113847
Gene: ENSMUSG00000040712
| Domain | Start | End | E-Value | Type |
|---|
|
CG-1
|
34 |
150 |
8.08e-88 |
SMART |
|
low complexity region
|
227 |
238 |
N/A |
INTRINSIC |
|
low complexity region
|
268 |
286 |
N/A |
INTRINSIC |
|
low complexity region
|
289 |
300 |
N/A |
INTRINSIC |
|
low complexity region
|
309 |
324 |
N/A |
INTRINSIC |
|
low complexity region
|
365 |
375 |
N/A |
INTRINSIC |
|
low complexity region
|
412 |
430 |
N/A |
INTRINSIC |
|
low complexity region
|
456 |
480 |
N/A |
INTRINSIC |
|
low complexity region
|
496 |
509 |
N/A |
INTRINSIC |
|
Pfam:TIG
|
536 |
616 |
1.1e-8 |
PFAM |
|
low complexity region
|
655 |
674 |
N/A |
INTRINSIC |
|
Blast:ANK
|
712 |
745 |
7e-12 |
BLAST |
|
SCOP:d1myo__
|
713 |
811 |
2e-15 |
SMART |
|
Blast:ANK
|
757 |
787 |
8e-13 |
BLAST |
|
low complexity region
|
824 |
834 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
848 |
N/A |
INTRINSIC |
|
low complexity region
|
856 |
877 |
N/A |
INTRINSIC |
|
IQ
|
1048 |
1070 |
2.59e2 |
SMART |
|
IQ
|
1071 |
1093 |
1.18e1 |
SMART |
|
IQ
|
1094 |
1116 |
5.42e0 |
SMART |
|
low complexity region
|
1128 |
1145 |
N/A |
INTRINSIC |
|
low complexity region
|
1168 |
1178 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000157027
|
| SMART Domains |
Protein: ENSMUSP00000115726
Gene: ENSMUSG00000060600
| Domain | Start | End | E-Value | Type |
|---|
|
Enolase_N
|
3 |
134 |
6.26e-91 |
SMART |
|
Pfam:Enolase_C
|
142 |
196 |
1e-29 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000120261
|
| SMART Domains |
Protein: ENSMUSP00000113667
Gene: ENSMUSG00000040712
| Domain | Start | End | E-Value | Type |
|---|
|
CG-1
|
34 |
126 |
3.23e-55 |
SMART |
|
low complexity region
|
203 |
214 |
N/A |
INTRINSIC |
|
low complexity region
|
244 |
262 |
N/A |
INTRINSIC |
|
low complexity region
|
265 |
276 |
N/A |
INTRINSIC |
|
low complexity region
|
285 |
300 |
N/A |
INTRINSIC |
|
low complexity region
|
341 |
351 |
N/A |
INTRINSIC |
|
low complexity region
|
388 |
406 |
N/A |
INTRINSIC |
|
low complexity region
|
432 |
456 |
N/A |
INTRINSIC |
|
low complexity region
|
472 |
485 |
N/A |
INTRINSIC |
|
Pfam:TIG
|
512 |
592 |
1e-8 |
PFAM |
|
low complexity region
|
631 |
650 |
N/A |
INTRINSIC |
|
Blast:ANK
|
688 |
721 |
7e-12 |
BLAST |
|
SCOP:d1myo__
|
689 |
787 |
2e-15 |
SMART |
|
Blast:ANK
|
733 |
763 |
7e-13 |
BLAST |
|
low complexity region
|
800 |
810 |
N/A |
INTRINSIC |
|
low complexity region
|
815 |
824 |
N/A |
INTRINSIC |
|
low complexity region
|
832 |
853 |
N/A |
INTRINSIC |
|
IQ
|
1024 |
1046 |
2.59e2 |
SMART |
|
IQ
|
1047 |
1063 |
2.38e2 |
SMART |
|
IQ
|
1077 |
1099 |
5.42e0 |
SMART |
|
low complexity region
|
1111 |
1128 |
N/A |
INTRINSIC |
|
low complexity region
|
1151 |
1161 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000179055
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000170716
|
| SMART Domains |
Protein: ENSMUSP00000128714
Gene: ENSMUSG00000060600
| Domain | Start | End | E-Value | Type |
|---|
|
Enolase_N
|
3 |
134 |
6.26e-91 |
SMART |
|
Enolase_C
|
142 |
431 |
8.8e-200 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000145823
|
| SMART Domains |
Protein: ENSMUSP00000123602
Gene: ENSMUSG00000040712
| Domain | Start | End | E-Value | Type |
|---|
|
CG-1
|
34 |
137 |
2.55e-44 |
SMART |
|
low complexity region
|
146 |
165 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.9491 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 95.0%
|
| Validation Efficiency |
96% (46/48) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 43 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Arhgap39 |
A |
T |
15: 76,609,712 (GRCm39) |
|
probably benign |
Het |
| Arrb1 |
G |
T |
7: 99,247,503 (GRCm39) |
|
probably benign |
Het |
| Cadps |
A |
G |
14: 12,822,323 (GRCm38) |
M139T |
probably damaging |
Het |
| Casr |
A |
G |
16: 36,320,703 (GRCm39) |
C482R |
probably benign |
Het |
| Cdca4 |
A |
T |
12: 112,785,499 (GRCm39) |
H76Q |
probably benign |
Het |
| Ddias |
A |
G |
7: 92,507,287 (GRCm39) |
L876P |
probably benign |
Het |
| Dnah9 |
T |
C |
11: 65,976,303 (GRCm39) |
S1249G |
possibly damaging |
Het |
| Dnttip1 |
A |
G |
2: 164,609,739 (GRCm39) |
|
probably benign |
Het |
| Eme2 |
A |
G |
17: 25,112,598 (GRCm39) |
S160P |
probably benign |
Het |
| Fbxw24 |
A |
T |
9: 109,437,256 (GRCm39) |
D210E |
probably damaging |
Het |
| Folr2 |
T |
C |
7: 101,489,881 (GRCm39) |
E129G |
probably damaging |
Het |
| Herc6 |
T |
A |
6: 57,636,664 (GRCm39) |
N793K |
possibly damaging |
Het |
| Iqcg |
T |
G |
16: 32,851,186 (GRCm39) |
K262Q |
possibly damaging |
Het |
| Lhfpl3 |
A |
G |
5: 22,980,690 (GRCm39) |
|
probably benign |
Het |
| Lmod2 |
A |
C |
6: 24,604,629 (GRCm39) |
S535R |
probably damaging |
Het |
| Lrp1b |
T |
A |
2: 40,555,094 (GRCm39) |
S342C |
possibly damaging |
Het |
| Lrrn3 |
T |
A |
12: 41,502,583 (GRCm39) |
Y578F |
possibly damaging |
Het |
| Map3k4 |
T |
A |
17: 12,467,885 (GRCm39) |
R1050W |
probably damaging |
Het |
| Muc6 |
T |
A |
7: 141,217,576 (GRCm39) |
T2301S |
possibly damaging |
Het |
| Mycbp2 |
T |
C |
14: 103,372,702 (GRCm39) |
E4048G |
probably damaging |
Het |
| Myh3 |
G |
C |
11: 66,975,858 (GRCm39) |
E297Q |
possibly damaging |
Het |
| Nkain3 |
A |
G |
4: 20,778,284 (GRCm39) |
V11A |
probably benign |
Het |
| Or4c102 |
G |
A |
2: 88,422,765 (GRCm39) |
V206I |
probably benign |
Het |
| P3h2 |
G |
C |
16: 25,924,040 (GRCm39) |
R132G |
possibly damaging |
Het |
| Pals2 |
C |
T |
6: 50,175,248 (GRCm39) |
Q520* |
probably null |
Het |
| Phgdh |
A |
G |
3: 98,221,591 (GRCm39) |
M447T |
probably benign |
Het |
| Pmfbp1 |
G |
A |
8: 110,258,695 (GRCm39) |
A667T |
probably benign |
Het |
| Pramel41 |
A |
T |
5: 94,593,720 (GRCm39) |
Q15L |
probably benign |
Het |
| Psmd2 |
T |
G |
16: 20,473,776 (GRCm39) |
C230G |
probably damaging |
Het |
| Rin2 |
C |
T |
2: 145,702,366 (GRCm39) |
T354I |
probably benign |
Het |
| Slc37a3 |
T |
A |
6: 39,315,747 (GRCm39) |
Y443F |
probably benign |
Het |
| Sorl1 |
C |
A |
9: 41,915,288 (GRCm39) |
G1314* |
probably null |
Het |
| St7 |
C |
T |
6: 17,854,932 (GRCm39) |
R267* |
probably null |
Het |
| Syne2 |
C |
T |
12: 76,141,167 (GRCm39) |
S99L |
probably damaging |
Het |
| Tacc2 |
T |
G |
7: 130,343,941 (GRCm39) |
S2533R |
possibly damaging |
Het |
| Tmem59l |
A |
G |
8: 70,939,951 (GRCm39) |
L6S |
unknown |
Het |
| Uaca |
T |
G |
9: 60,777,173 (GRCm39) |
V518G |
probably damaging |
Het |
| Usp43 |
T |
C |
11: 67,746,716 (GRCm39) |
E992G |
probably benign |
Het |
| Vmn1r55 |
A |
G |
7: 5,150,075 (GRCm39) |
V116A |
probably benign |
Het |
| Wdr3 |
G |
A |
3: 100,047,543 (GRCm39) |
T844M |
probably benign |
Het |
| Wdr7 |
T |
A |
18: 63,911,320 (GRCm39) |
M904K |
probably damaging |
Het |
| Zbtb39 |
A |
G |
10: 127,578,696 (GRCm39) |
I423M |
possibly damaging |
Het |
| Zmym1 |
A |
T |
4: 126,941,897 (GRCm39) |
C830* |
probably null |
Het |
|
| Other mutations in Spag7 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01764:Spag7
|
APN |
11 |
70,554,933 (GRCm39) |
unclassified |
probably benign |
|
|
life_savers
|
UTSW |
11 |
70,555,688 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0371:Spag7
|
UTSW |
11 |
70,555,622 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0376:Spag7
|
UTSW |
11 |
70,560,016 (GRCm39) |
unclassified |
probably benign |
|
|
R0973:Spag7
|
UTSW |
11 |
70,560,008 (GRCm39) |
unclassified |
probably benign |
|
|
R1622:Spag7
|
UTSW |
11 |
70,555,688 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1955:Spag7
|
UTSW |
11 |
70,555,844 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4025:Spag7
|
UTSW |
11 |
70,555,300 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4385:Spag7
|
UTSW |
11 |
70,560,029 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4409:Spag7
|
UTSW |
11 |
70,555,688 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4561:Spag7
|
UTSW |
11 |
70,555,816 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5493:Spag7
|
UTSW |
11 |
70,560,059 (GRCm39) |
missense |
probably null |
1.00 |
|
R6366:Spag7
|
UTSW |
11 |
70,555,418 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R7283:Spag7
|
UTSW |
11 |
70,556,139 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7653:Spag7
|
UTSW |
11 |
70,555,691 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8404:Spag7
|
UTSW |
11 |
70,560,059 (GRCm39) |
missense |
probably benign |
0.15 |
|
R8502:Spag7
|
UTSW |
11 |
70,560,059 (GRCm39) |
missense |
probably benign |
0.15 |
|
R9628:Spag7
|
UTSW |
11 |
70,555,186 (GRCm39) |
missense |
probably benign |
0.04 |
|
| Predicted Primers |
PCR Primer
(F):5'- GTGCAAACTCCTGGAGATGAAG -3'
(R):5'- GTCTGATTTCATCCAGGACAGTG -3'
Sequencing Primer
(F):5'- CTCCTGGAGATGAAGAGAAAGGTAG -3'
(R):5'- TCCAGGACAGTGGACAGGTC -3'
|
| Posted On |
2015-07-07 |
Incidental Mutation