Incidental Mutation 'R4347:Igkv10-96'
ID328365
Institutional Source Beutler Lab
Gene Symbol Igkv10-96
Ensembl Gene ENSMUSG00000094420
Gene Nameimmunoglobulin kappa variable 10-96
SynonymsGm16637
MMRRC Submission 041102-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.237) question?
Stock #R4347 (G1)
Quality Score225
Status Validated
Chromosome6
Chromosomal Location68631965-68632430 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 68632180 bp
ZygosityHeterozygous
Amino Acid Change Arginine to Tryptophan at position 44 (R44W)
Ref Sequence ENSEMBL: ENSMUSP00000100129 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000103328]
Predicted Effect probably benign
Transcript: ENSMUST00000103328
AA Change: R44W

PolyPhen 2 Score 0.100 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000100129
Gene: ENSMUSG00000094420
AA Change: R44W

DomainStartEndE-ValueType
low complexity region 8 13 N/A INTRINSIC
IGv 38 110 1.94e-19 SMART
Meta Mutation Damage Score 0.152 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 94.9%
Validation Efficiency 100% (32/32)
Allele List at MGI
Other mutations in this stock
Total: 25 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4921536K21Rik C T 11: 3,890,122 V92M probably damaging Het
4930503E14Rik C A 14: 44,171,178 R66S probably damaging Het
Abca5 A T 11: 110,299,968 I772N probably damaging Het
Acox1 T C 11: 116,198,661 N15S probably benign Het
Arhgap28 C T 17: 67,873,142 V233I probably benign Het
Chil4 T C 3: 106,202,828 I317V probably benign Het
Dvl3 G A 16: 20,531,299 R645H possibly damaging Het
Efl1 A T 7: 82,697,966 M567L probably damaging Het
Gal3st2b C T 1: 93,939,808 T59I probably damaging Het
Gm7135 T A 1: 97,348,310 noncoding transcript Het
Gpihbp1 T G 15: 75,598,168 *124G probably null Het
Igkv13-84 T A 6: 68,939,776 I19K probably benign Het
Kcnab1 A G 3: 65,297,475 probably benign Het
Kif1b C A 4: 149,247,234 G545C probably damaging Het
Mprip T C 11: 59,759,453 S1328P possibly damaging Het
Nrp1 T C 8: 128,480,991 probably null Het
Olfml1 C T 7: 107,567,833 P23L probably benign Het
Plekhh2 G A 17: 84,619,702 A1457T probably benign Het
Prr27 A G 5: 87,842,672 I40V possibly damaging Het
Slc25a54 A G 3: 109,102,739 T185A possibly damaging Het
Srek1 C T 13: 103,748,759 G396D probably null Het
Syvn1 C T 19: 6,049,921 probably benign Het
Trim3 C T 7: 105,619,387 G120D probably damaging Het
Usp5 T A 6: 124,821,195 Q409L probably damaging Het
Vim T G 2: 13,575,518 probably benign Het
Other mutations in Igkv10-96
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01456:Igkv10-96 APN 6 68632102 missense probably benign 0.04
R1555:Igkv10-96 UTSW 6 68632381 critical splice donor site probably benign
R5229:Igkv10-96 UTSW 6 68632239 missense possibly damaging 0.88
R6396:Igkv10-96 UTSW 6 68631985 nonsense probably null
R6623:Igkv10-96 UTSW 6 68632174 missense probably damaging 0.97
R7183:Igkv10-96 UTSW 6 68632216 missense probably benign 0.39
R7194:Igkv10-96 UTSW 6 68632044 missense possibly damaging 0.80
Predicted Primers PCR Primer
(F):5'- GCTCCAGGTTGCTAATGGTG -3'
(R):5'- GACATGATGTCCTCTGCTCAG -3'

Sequencing Primer
(F):5'- GTGAGAGAATAATCTGTTCCAGACCC -3'
(R):5'- ACTACAATGGGAATTTTGCTGTTGC -3'
Posted On2015-07-07