Mutagenetix > Incidental Mutation

Incidental Mutation 'R4433:Or4g7'

328676

Institutional Source

Beutler Lab

Gene Symbol

Or4g7

Ensembl Gene

ENSMUSG00000044039

Gene Name

olfactory receptor family 4 subfamily G member 7

Synonyms

Olfr1288, GA_x6K02T2Q125-72530279-72531217, MOR245-9

MMRRC Submission

041147-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R4433 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

111309131-111310069 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 111309757 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 209 (C209*)

Ref Sequence

ENSEMBL: ENSMUSP00000150331 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000104889] [ENSMUST00000120021] [ENSMUST00000207494] [ENSMUST00000214816]

AlphaFold

Q7TQY0

Predicted Effect

probably null
Transcript: ENSMUST00000104889
AA Change: C209*

SMART Domains

Protein: ENSMUSP00000100485
Gene: ENSMUSG00000044039
AA Change: C209*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	1.3e-43	PFAM
Pfam:7tm_1	41	287	2.5e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000120021

Predicted Effect

probably benign
Transcript: ENSMUST00000207494

Predicted Effect

probably null
Transcript: ENSMUST00000214816
AA Change: C209*

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225425

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

98% (59/60)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc5	A	T	16: 20,186,937 (GRCm39)		probably null	Het
Acsm2	A	G	7: 119,153,732 (GRCm39)	H14R	unknown	Het
Adamtsl4	T	C	3: 95,589,069 (GRCm39)		probably null	Het
Ak5	A	G	3: 152,361,517 (GRCm39)	I135T	probably damaging	Het
Alk	G	T	17: 72,206,236 (GRCm39)	S1038*	probably null	Het
Ank2	A	T	3: 126,741,455 (GRCm39)		probably benign	Het
Ap2m1	A	T	16: 20,362,134 (GRCm39)	H414L	possibly damaging	Het
Atp13a5	A	T	16: 29,100,842 (GRCm39)	M649K	probably damaging	Het
Atp4a	G	A	7: 30,419,650 (GRCm39)	R671Q	probably benign	Het
Calhm6	T	A	10: 34,003,827 (GRCm39)	T27S	probably damaging	Het
Cdc25b	A	G	2: 131,033,618 (GRCm39)	S186G	probably benign	Het
Ceacam16	A	G	7: 19,587,514 (GRCm39)	V418A	possibly damaging	Het
Cntnap3	A	C	13: 64,926,667 (GRCm39)	S568A	possibly damaging	Het
Col24a1	G	T	3: 145,020,144 (GRCm39)	V172F	possibly damaging	Het
Dynlt4	C	T	4: 116,985,320 (GRCm39)	R48C	probably damaging	Het
Eef2	CCC	CCCC	10: 81,014,602 (GRCm39)		probably null	Het
Esp36	T	A	17: 38,729,847 (GRCm39)	T15S	unknown	Het
Fat2	A	T	11: 55,200,466 (GRCm39)	H869Q	possibly damaging	Het
Fat3	A	G	9: 15,942,448 (GRCm39)	V1308A	probably damaging	Het
Gimap3	T	C	6: 48,742,880 (GRCm39)	T17A	possibly damaging	Het
Hnrnpr	A	G	4: 136,044,459 (GRCm39)	K13R	probably benign	Het
Hycc1	A	C	5: 24,184,579 (GRCm39)	C218G	possibly damaging	Het
Kdr	A	G	5: 76,104,585 (GRCm39)	M1133T	possibly damaging	Het
Mgarp	A	G	3: 51,303,681 (GRCm39)		probably benign	Het
Neto2	G	A	8: 86,367,712 (GRCm39)	T337I	probably damaging	Het
Nfib	C	T	4: 82,416,672 (GRCm39)	R137Q	probably damaging	Het
Nherf4	T	C	9: 44,159,285 (GRCm39)	*499W	probably null	Het
Nr3c2	A	G	8: 77,944,096 (GRCm39)	E890G	probably damaging	Het
Nsun4	A	G	4: 115,897,327 (GRCm39)	V228A	possibly damaging	Het
Nt5c1a	T	A	4: 123,109,689 (GRCm39)	S263T	probably benign	Het
Ntm	A	T	9: 28,923,516 (GRCm39)	Y45*	probably null	Het
Nts	A	G	10: 102,320,888 (GRCm39)	V67A	probably benign	Het
Or1e19	A	T	11: 73,316,537 (GRCm39)	S91T	possibly damaging	Het
Or1e25	A	G	11: 73,493,712 (GRCm39)	Y102C	probably damaging	Het
Or51ag1	T	A	7: 103,155,346 (GRCm39)	K269M	probably benign	Het
Ostm1	C	A	10: 42,555,119 (GRCm39)	A47E	probably benign	Het
Otol1	G	A	3: 69,925,881 (GRCm39)	V19M	probably benign	Het
Pcdhb15	A	G	18: 37,608,565 (GRCm39)	N599S	probably damaging	Het
Pcdhgb1	T	C	18: 37,814,304 (GRCm39)	I265T	probably damaging	Het
Pex14	T	C	4: 149,045,967 (GRCm39)	E321G	possibly damaging	Het
Phactr3	C	A	2: 177,924,925 (GRCm39)	R251S	probably damaging	Het
Pkdcc	C	T	17: 83,528,570 (GRCm39)	T313M	probably benign	Het
Plce1	A	T	19: 38,755,745 (GRCm39)	E1911V	probably damaging	Het
Ptprv	G	T	1: 135,042,308 (GRCm39)		noncoding transcript	Het
Rab36	G	A	10: 74,880,328 (GRCm39)	V63I	probably damaging	Het
Rhob	A	G	12: 8,549,533 (GRCm39)	Y34H	possibly damaging	Het
Slc27a3	G	A	3: 90,294,647 (GRCm39)	T408M	probably damaging	Het
Slc9c1	A	T	16: 45,419,829 (GRCm39)	I1000F	possibly damaging	Het
Tcf7	G	T	11: 52,152,442 (GRCm39)	P36T	probably benign	Het
Tcf7l1	C	G	6: 72,765,752 (GRCm39)	E62Q	probably damaging	Het
Tll2	A	G	19: 41,109,787 (GRCm39)	S326P	probably benign	Het
Tubgcp4	A	G	2: 121,014,954 (GRCm39)	N288S	probably benign	Het
Zfhx3	G	A	8: 109,682,269 (GRCm39)	R3236H	unknown	Het
Zfp764l1	T	G	7: 126,992,174 (GRCm39)	Q87P	possibly damaging	Het
Zgrf1	A	G	3: 127,355,727 (GRCm39)	T318A	probably benign	Het

Other mutations in Or4g7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Or4g7	APN	2	111,309,209 (GRCm39)	missense	probably benign
IGL02021:Or4g7	APN	2	111,309,825 (GRCm39)	missense	probably benign	0.03
IGL02061:Or4g7	APN	2	111,309,614 (GRCm39)	missense	possibly damaging	0.94
R2016:Or4g7	UTSW	2	111,309,532 (GRCm39)	missense	probably benign	0.00
R2017:Or4g7	UTSW	2	111,309,532 (GRCm39)	missense	probably benign	0.00
R2848:Or4g7	UTSW	2	111,309,699 (GRCm39)	missense	probably benign	0.00
R2849:Or4g7	UTSW	2	111,309,699 (GRCm39)	missense	probably benign	0.00
R3421:Or4g7	UTSW	2	111,309,297 (GRCm39)	missense	probably benign	0.12
R4223:Or4g7	UTSW	2	111,309,489 (GRCm39)	missense	probably benign	0.00
R4432:Or4g7	UTSW	2	111,309,757 (GRCm39)	nonsense	probably null
R4476:Or4g7	UTSW	2	111,310,009 (GRCm39)	missense	possibly damaging	0.58
R4631:Or4g7	UTSW	2	111,309,908 (GRCm39)	missense	probably damaging	1.00
R6029:Or4g7	UTSW	2	111,309,310 (GRCm39)	nonsense	probably null
R6036:Or4g7	UTSW	2	111,309,333 (GRCm39)	missense	probably damaging	1.00
R6036:Or4g7	UTSW	2	111,309,333 (GRCm39)	missense	probably damaging	1.00
R6084:Or4g7	UTSW	2	111,309,734 (GRCm39)	missense	probably damaging	1.00
R6329:Or4g7	UTSW	2	111,309,573 (GRCm39)	missense	possibly damaging	0.90
R7307:Or4g7	UTSW	2	111,309,105 (GRCm39)	start gained	probably benign
R7516:Or4g7	UTSW	2	111,309,282 (GRCm39)	missense	probably benign	0.01
R7577:Or4g7	UTSW	2	111,309,477 (GRCm39)	missense	probably damaging	0.98
R8108:Or4g7	UTSW	2	111,309,579 (GRCm39)	missense	possibly damaging	0.90
R8210:Or4g7	UTSW	2	111,309,753 (GRCm39)	missense	possibly damaging	0.89
R8465:Or4g7	UTSW	2	111,309,425 (GRCm39)	missense	probably benign	0.01
R8717:Or4g7	UTSW	2	111,309,992 (GRCm39)	missense	probably damaging	1.00
R8730:Or4g7	UTSW	2	111,309,934 (GRCm39)	missense	probably damaging	1.00
R9360:Or4g7	UTSW	2	111,309,684 (GRCm39)	missense	probably benign	0.10
Z1177:Or4g7	UTSW	2	111,309,552 (GRCm39)	missense	probably damaging	1.00
Z1177:Or4g7	UTSW	2	111,309,159 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- AAGCCTCTTCACTACCTGATGATC -3'
(R):5'- ACTTGGTCGGAAATGGCCATAC -3'

Sequencing Primer
(F):5'- TCACTACCTGATGATCATGAATCC -3'
(R):5'- TCGGAAATGGCCATACATACAG -3'

Posted On

2015-07-21