Mutagenetix > Incidental Mutation

Incidental Mutation 'R4434:Or10j5'

328732

Institutional Source

Beutler Lab

Gene Symbol

Or10j5

Ensembl Gene

ENSMUSG00000037924

Gene Name

olfactory receptor family 10 subfamily J member 5

Synonyms

Olfr16, MOR267-13, GA_x6K02T2R7CC-893157-892228, MOR23

MMRRC Submission

041148-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R4434 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

172784335-172785384 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 172785111 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Tyrosine at position 250 (H250Y)

Ref Sequence

ENSEMBL: ENSMUSP00000149249 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038432] [ENSMUST00000215254]

AlphaFold

Q62007

Predicted Effect

probably damaging
Transcript: ENSMUST00000038432
AA Change: H250Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000041524
Gene: ENSMUSG00000037924
AA Change: H250Y

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	7.5e-51	PFAM
Pfam:7tm_1	41	289	3.4e-22	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215254
AA Change: H250Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars1	A	T	8: 111,781,253 (GRCm39)	Q907L	probably null	Het
Adam6b	A	C	12: 113,454,281 (GRCm39)	Q366P	probably damaging	Het
AI597479	C	T	1: 43,139,959 (GRCm39)	Q62*	probably null	Het
Akap9	T	A	5: 4,082,708 (GRCm39)	M1944K	probably damaging	Het
Akr1c19	T	C	13: 4,292,615 (GRCm39)	V212A	probably benign	Het
Ank3	C	T	10: 69,822,900 (GRCm39)	S523L	probably damaging	Het
Antxrl	G	A	14: 33,793,574 (GRCm39)		probably benign	Het
Arhgap21	A	G	2: 20,972,146 (GRCm39)	C26R	probably benign	Het
Cdc37l1	T	C	19: 28,985,021 (GRCm39)	F224L	probably damaging	Het
Csmd3	G	A	15: 47,763,191 (GRCm39)	T1215I	possibly damaging	Het
Dnah7c	G	A	1: 46,705,442 (GRCm39)	R2485H	probably damaging	Het
Dnah9	T	A	11: 65,998,901 (GRCm39)	N1049I	possibly damaging	Het
Dnaja3	T	G	16: 4,507,859 (GRCm39)	Y120*	probably null	Het
Ehbp1l1	G	T	19: 5,766,276 (GRCm39)	R412S	possibly damaging	Het
Fam169a	A	G	13: 97,263,248 (GRCm39)	D567G	probably damaging	Het
Gcnt4	T	A	13: 97,082,850 (GRCm39)	Y49N	probably benign	Het
Gm5849	T	A	3: 90,685,182 (GRCm39)	K1M	probably null	Het
Gngt1	A	G	6: 3,994,282 (GRCm39)	D20G	probably benign	Het
Gnptab	A	G	10: 88,248,484 (GRCm39)	N104S	probably damaging	Het
Gpn3	A	G	5: 122,520,115 (GRCm39)	D223G	probably benign	Het
Hectd1	A	T	12: 51,798,835 (GRCm39)	I2095K	probably damaging	Het
Hephl1	C	T	9: 14,988,092 (GRCm39)	R672H	probably damaging	Het
Hpse2	A	G	19: 43,282,708 (GRCm39)	S182P	probably benign	Het
Itgb4	T	A	11: 115,890,640 (GRCm39)	S1109R	probably benign	Het
Itgbl1	A	T	14: 124,209,611 (GRCm39)	D330V	probably damaging	Het
Krt77	A	T	15: 101,773,904 (GRCm39)	V250E	probably damaging	Het
Mycbp2	T	A	14: 103,371,225 (GRCm39)	N4108Y	probably damaging	Het
Nampt	T	C	12: 32,888,362 (GRCm39)	I171T	probably damaging	Het
Nckap5l	A	C	15: 99,320,744 (GRCm39)	S1270A	probably benign	Het
Opa1	A	G	16: 29,430,801 (GRCm39)	I500M	probably damaging	Het
Pax8	G	A	2: 24,319,621 (GRCm39)	P350L	possibly damaging	Het
Plxnb2	A	G	15: 89,047,006 (GRCm39)	C772R	probably damaging	Het
Prh1	A	T	6: 132,548,841 (GRCm39)	H116L	unknown	Het
Rgsl1	C	T	1: 153,678,087 (GRCm39)	A114T	possibly damaging	Het
Rln1	A	T	19: 29,311,962 (GRCm39)	F12Y	possibly damaging	Het
Robo4	CGG	CG	9: 37,322,786 (GRCm39)		probably null	Het
Scube1	A	T	15: 83,606,125 (GRCm39)	I49N	probably damaging	Het
Shank3	C	A	15: 89,387,562 (GRCm39)	L244I	probably damaging	Het
Shc3	T	C	13: 51,603,302 (GRCm39)	T268A	probably benign	Het
Sp7	A	G	15: 102,267,536 (GRCm39)	L90P	probably damaging	Het
Tacc2	G	A	7: 130,225,271 (GRCm39)	S652N	probably damaging	Het
Tacstd2	A	G	6: 67,512,128 (GRCm39)	V188A	possibly damaging	Het
Tasor	G	A	14: 27,171,818 (GRCm39)		probably null	Het
Tex26	T	C	5: 149,376,820 (GRCm39)	S70P	probably benign	Het
Tmc4	C	T	7: 3,675,006 (GRCm39)	V222M	probably benign	Het
Tnc	T	C	4: 63,926,066 (GRCm39)	T905A	possibly damaging	Het
Trim65	G	A	11: 116,018,435 (GRCm39)	Q253*	probably null	Het
Tuba3a	A	T	6: 125,258,506 (GRCm39)	Y161*	probably null	Het
Zfand2b	A	G	1: 75,147,330 (GRCm39)	S197G	possibly damaging	Het
Zfp109	T	A	7: 23,928,771 (GRCm39)	T213S	probably benign	Het
Zfp985	A	C	4: 147,668,368 (GRCm39)	D412A	probably benign	Het
Zswim3	T	A	2: 164,662,563 (GRCm39)	C348S	probably benign	Het

Other mutations in Or10j5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00334:Or10j5	APN	1	172,785,158 (GRCm39)	missense	possibly damaging	0.66
IGL00336:Or10j5	APN	1	172,785,045 (GRCm39)	missense	probably benign	0.30
IGL01155:Or10j5	APN	1	172,784,491 (GRCm39)	missense	probably benign	0.43
IGL01549:Or10j5	APN	1	172,784,541 (GRCm39)	missense	probably damaging	1.00
IGL02679:Or10j5	APN	1	172,784,743 (GRCm39)	missense	probably damaging	1.00
IGL03071:Or10j5	APN	1	172,784,502 (GRCm39)	missense	probably benign	0.01
IGL03352:Or10j5	APN	1	172,784,850 (GRCm39)	missense	probably benign	0.00
R0449:Or10j5	UTSW	1	172,784,965 (GRCm39)	missense	probably damaging	1.00
R1725:Or10j5	UTSW	1	172,784,908 (GRCm39)	missense	possibly damaging	0.90
R1726:Or10j5	UTSW	1	172,784,658 (GRCm39)	missense	probably benign	0.00
R1735:Or10j5	UTSW	1	172,784,374 (GRCm39)	missense	probably benign
R1928:Or10j5	UTSW	1	172,784,881 (GRCm39)	missense	probably damaging	0.98
R4258:Or10j5	UTSW	1	172,785,205 (GRCm39)	missense	possibly damaging	0.88
R4359:Or10j5	UTSW	1	172,784,647 (GRCm39)	missense	probably benign
R4666:Or10j5	UTSW	1	172,785,157 (GRCm39)	missense	probably benign
R4874:Or10j5	UTSW	1	172,785,166 (GRCm39)	missense	probably benign	0.00
R5063:Or10j5	UTSW	1	172,785,009 (GRCm39)	missense	possibly damaging	0.48
R5988:Or10j5	UTSW	1	172,784,723 (GRCm39)	nonsense	probably null
R6074:Or10j5	UTSW	1	172,784,945 (GRCm39)	missense	probably benign	0.10
R7021:Or10j5	UTSW	1	172,784,494 (GRCm39)	missense	probably benign	0.01
R7234:Or10j5	UTSW	1	172,784,673 (GRCm39)	missense	probably damaging	0.96
R7527:Or10j5	UTSW	1	172,784,511 (GRCm39)	missense	probably benign	0.00
R8271:Or10j5	UTSW	1	172,784,744 (GRCm39)	nonsense	probably null
R8890:Or10j5	UTSW	1	172,785,045 (GRCm39)	missense	probably benign	0.30
R8906:Or10j5	UTSW	1	172,784,186 (GRCm39)	start gained	probably benign
R9048:Or10j5	UTSW	1	172,784,929 (GRCm39)	missense	probably benign
R9583:Or10j5	UTSW	1	172,784,893 (GRCm39)	missense	probably damaging	1.00
Z1088:Or10j5	UTSW	1	172,784,891 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATTTGCCCTTTTGTGGCACG -3'
(R):5'- AGAAGTGTTTCTGCCCACAGC -3'

Sequencing Primer
(F):5'- CCCAGTAATGAAGCTTTCTTGTG -3'
(R):5'- TGCCCACAGCTCTGCATAG -3'

Posted On

2015-07-21