Mutagenetix > Incidental Mutation

Incidental Mutation 'R4450:Lzts2'

328947

Institutional Source

Beutler Lab

Gene Symbol

Lzts2

Ensembl Gene

ENSMUSG00000035342

Gene Name

leucine zipper, putative tumor suppressor 2

Synonyms

MMRRC Submission

041711-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R4450 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

45003615-45015554 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 45012032 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 154 (K154*)

Ref Sequence

ENSEMBL: ENSMUSP00000137571 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039016] [ENSMUST00000145391] [ENSMUST00000169459] [ENSMUST00000178087] [ENSMUST00000179108]

AlphaFold

Q91YU6

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000038901

Predicted Effect

probably null
Transcript: ENSMUST00000039016
AA Change: K154*

SMART Domains

Protein: ENSMUSP00000045478
Gene: ENSMUSG00000035342
AA Change: K154*

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	639	4.2e-82	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000145391

SMART Domains

Protein: ENSMUSP00000119002
Gene: ENSMUSG00000074818

Domain	Start	End	E-Value	Type
low complexity region	12	35	N/A	INTRINSIC
PDZ	95	167	3.51e-19	SMART
PDZ	220	292	2.47e-14	SMART
low complexity region	319	344	N/A	INTRINSIC
low complexity region	442	459	N/A	INTRINSIC
low complexity region	521	533	N/A	INTRINSIC
low complexity region	724	744	N/A	INTRINSIC
low complexity region	768	809	N/A	INTRINSIC
low complexity region	812	824	N/A	INTRINSIC
PDZ	866	947	1.96e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169459

SMART Domains

Protein: ENSMUSP00000133273
Gene: ENSMUSG00000074818

Domain	Start	End	E-Value	Type
low complexity region	12	35	N/A	INTRINSIC
PDZ	95	167	3.51e-19	SMART
PDZ	220	292	2.47e-14	SMART
low complexity region	319	344	N/A	INTRINSIC
low complexity region	442	459	N/A	INTRINSIC
low complexity region	521	533	N/A	INTRINSIC
low complexity region	724	744	N/A	INTRINSIC
low complexity region	768	809	N/A	INTRINSIC
low complexity region	812	824	N/A	INTRINSIC
PDZ	866	947	1.96e-8	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000178087
AA Change: K154*

SMART Domains

Protein: ENSMUSP00000136405
Gene: ENSMUSG00000035342
AA Change: K154*

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	638	2.2e-66	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000179108
AA Change: K154*

SMART Domains

Protein: ENSMUSP00000137571
Gene: ENSMUSG00000035342
AA Change: K154*

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	639	4.2e-82	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

98% (43/44)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the leucine zipper tumor suppressor family of proteins, which function in transcription regulation and cell cycle control. This family member can repress beta-catenin-mediated transcriptional activation and is a negative regulator of the Wnt signaling pathway. It negatively regulates microtubule severing at centrosomes, and is necessary for central spindle formation and cytokinesis completion. It is implicated in cancer, where it may inhibit cell proliferation and decrease susceptibility to tumor development. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for a null mutation display defects in urinary tract development. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3930402G23Rik	T	A	8: 10,976,129 (GRCm39)		noncoding transcript	Het
Acsl6	A	G	11: 54,219,229 (GRCm39)	D278G	probably damaging	Het
Adamdec1	C	T	14: 68,810,568 (GRCm39)	R196Q	probably benign	Het
Akap13	T	A	7: 75,392,508 (GRCm39)	F2450L	probably damaging	Het
Alg11	C	A	8: 22,558,095 (GRCm39)	A469E	probably benign	Het
Angel1	A	T	12: 86,768,698 (GRCm39)	Y262N	probably damaging	Het
Arhgef18	A	G	8: 3,487,097 (GRCm39)	E272G	probably damaging	Het
Bpifb6	A	G	2: 153,748,688 (GRCm39)	E228G	possibly damaging	Het
Brca2	T	G	5: 150,459,518 (GRCm39)	D264E	probably damaging	Het
Cdca4	T	C	12: 112,785,278 (GRCm39)	N150S	probably benign	Het
Cep162	T	C	9: 87,107,861 (GRCm39)	S510G	probably damaging	Het
Cldn12	T	C	5: 5,558,398 (GRCm39)	T10A	probably damaging	Het
Clip2	C	T	5: 134,531,807 (GRCm39)	G631D	possibly damaging	Het
Col19a1	T	A	1: 24,361,116 (GRCm39)	T625S	probably damaging	Het
Col6a5	C	T	9: 105,781,720 (GRCm39)	G1635D	unknown	Het
Dcp1b	A	G	6: 119,183,437 (GRCm39)	T175A	probably benign	Het
Eln	C	T	5: 134,754,635 (GRCm39)		probably benign	Het
Galnt2	A	G	8: 125,022,116 (GRCm39)	D14G	probably benign	Het
Gm9894	T	A	13: 67,913,199 (GRCm39)		noncoding transcript	Het
Herc2	T	C	7: 55,877,640 (GRCm39)	L4569P	probably damaging	Het
Iqcm	T	C	8: 76,356,394 (GRCm39)	S176P	probably damaging	Het
Klhl42	G	A	6: 146,993,169 (GRCm39)	G47D	probably benign	Het
Lmo2	T	C	2: 103,811,407 (GRCm39)	Y147H	probably damaging	Het
Map4k3	C	T	17: 80,911,411 (GRCm39)		probably null	Het
Mlc1	A	G	15: 88,847,693 (GRCm39)	F285S	probably benign	Het
Myo5a	T	A	9: 75,074,458 (GRCm39)	M789K	probably benign	Het
Nbeal1	T	A	1: 60,306,933 (GRCm39)	S319T	probably damaging	Het
Nsun4	A	T	4: 115,908,453 (GRCm39)	Y702*	probably null	Het
Or8b52	A	T	9: 38,577,050 (GRCm39)	V30E	probably benign	Het
Or8g34	G	A	9: 39,373,328 (GRCm39)	M200I	probably benign	Het
Osbpl5	T	A	7: 143,248,643 (GRCm39)	T640S	probably benign	Het
Plaat1	C	T	16: 29,046,976 (GRCm39)	T165M	possibly damaging	Het
Rangrf	A	T	11: 68,866,010 (GRCm39)		probably benign	Het
Rnpc3	T	C	3: 113,404,786 (GRCm39)		probably benign	Het
Ros1	C	T	10: 51,954,038 (GRCm39)	G1867D	probably damaging	Het
Slc11a1	C	A	1: 74,424,694 (GRCm39)		probably benign	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Speer3	C	G	5: 13,846,394 (GRCm39)	A238G	possibly damaging	Het
Syt6	A	G	3: 103,492,961 (GRCm39)	H156R	probably benign	Het
Tln2	C	T	9: 67,251,347 (GRCm39)		probably null	Het
Trim58	T	C	11: 58,542,191 (GRCm39)	W384R	probably benign	Het
Wwc2	A	G	8: 48,321,702 (GRCm39)	Y471H	unknown	Het

Other mutations in Lzts2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02115:Lzts2	APN	19	45,014,809 (GRCm39)	unclassified	probably benign
R0053:Lzts2	UTSW	19	45,014,746 (GRCm39)	unclassified	probably benign
R0135:Lzts2	UTSW	19	45,014,626 (GRCm39)	unclassified	probably benign
R0433:Lzts2	UTSW	19	45,010,115 (GRCm39)	missense	possibly damaging	0.87
R1079:Lzts2	UTSW	19	45,011,983 (GRCm39)	missense	probably damaging	1.00
R1459:Lzts2	UTSW	19	45,009,893 (GRCm39)	missense	probably damaging	1.00
R2869:Lzts2	UTSW	19	45,012,534 (GRCm39)	nonsense	probably null
R2869:Lzts2	UTSW	19	45,012,534 (GRCm39)	nonsense	probably null
R5806:Lzts2	UTSW	19	45,014,806 (GRCm39)	unclassified	probably benign
R6958:Lzts2	UTSW	19	45,012,582 (GRCm39)	unclassified	probably benign
R7598:Lzts2	UTSW	19	45,012,272 (GRCm39)	nonsense	probably null
R9398:Lzts2	UTSW	19	45,013,208 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GGGCTTGGTATGACACACC -3'
(R):5'- ACTGGCCCAACCACTCAGT -3'

Sequencing Primer
(F):5'- GTAGCTGTCTTTGAACACACCAG -3'
(R):5'- CACTCAGTGCCAAGGGTTTGTC -3'

Posted On

2015-07-21