Mutagenetix > Incidental Mutation

Incidental Mutation 'R4438:Vmn2r25'

329624

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r25

Ensembl Gene

ENSMUSG00000094672

Gene Name

vomeronasal 2, receptor 25

Synonyms

EG545874

MMRRC Submission

041703-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.140) question?

Stock #

R4438 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

123799773-123830149 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 123816756 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 275 (I275T)

Ref Sequence

ENSEMBL: ENSMUSP00000124342 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000162046]

AlphaFold

W4VSP2

Predicted Effect

probably benign
Transcript: ENSMUST00000162046
AA Change: I275T

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000124342
Gene: ENSMUSG00000094672
AA Change: I275T

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	473	6e-31	PFAM
Pfam:NCD3G	519	572	5.8e-25	PFAM
Pfam:7tm_3	603	840	4.8e-55	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acvr1	T	A	2: 58,367,739 (GRCm39)	I141F	probably benign	Het
Adam6b	A	C	12: 113,454,281 (GRCm39)	Q366P	probably damaging	Het
Adck1	C	T	12: 88,397,920 (GRCm39)	Q185*	probably null	Het
Adgrb3	T	C	1: 25,870,108 (GRCm39)		probably benign	Het
Als2cl	T	C	9: 110,714,466 (GRCm39)	S105P	probably damaging	Het
Asns	C	A	6: 7,675,320 (GRCm39)	A561S	probably benign	Het
Bcl2a1d	A	T	9: 88,613,753 (GRCm39)	M7K	probably benign	Het
Bst1	A	T	5: 43,982,682 (GRCm39)		probably null	Het
Cdc37l1	T	C	19: 28,985,021 (GRCm39)	F224L	probably damaging	Het
Csmd3	G	A	15: 47,763,191 (GRCm39)	T1215I	possibly damaging	Het
Csnk1e	A	T	15: 79,305,129 (GRCm39)	S323T	probably benign	Het
Cyp2j7	G	T	4: 96,105,646 (GRCm39)	T288K	probably benign	Het
Dnaaf5	T	A	5: 139,149,147 (GRCm39)	I482N	probably damaging	Het
Dpy19l3	A	G	7: 35,392,284 (GRCm39)	I636T	probably damaging	Het
Enpep	T	G	3: 129,077,740 (GRCm39)	T626P	possibly damaging	Het
Fgfr2	G	A	7: 129,774,660 (GRCm39)	R509*	probably null	Het
Gm5849	T	A	3: 90,685,182 (GRCm39)	K1M	probably null	Het
Gpr83	T	C	9: 14,776,134 (GRCm39)	Y152H	probably damaging	Het
Hdac7	T	C	15: 97,705,596 (GRCm39)	E400G	probably damaging	Het
Hypk	A	T	2: 121,288,475 (GRCm39)	E82V	probably damaging	Het
Iqub	C	A	6: 24,505,867 (GRCm39)	A14S	probably benign	Het
Lias	T	C	5: 65,552,787 (GRCm39)	M130T	probably damaging	Het
Lingo4	T	C	3: 94,310,204 (GRCm39)	S381P	possibly damaging	Het
Mdn1	A	G	4: 32,704,635 (GRCm39)	T1536A	probably damaging	Het
Moxd2	T	A	6: 40,860,996 (GRCm39)	D265V	probably damaging	Het
Mtbp	C	A	15: 55,466,611 (GRCm39)	S541R	probably benign	Het
Or10j27	C	A	1: 172,957,869 (GRCm39)	C305F	probably benign	Het
Or2a54	T	C	6: 43,093,221 (GRCm39)	S182P	probably benign	Het
Or4k15b	T	C	14: 50,272,287 (GRCm39)	D191G	probably damaging	Het
Pcsk9	G	T	4: 106,316,156 (GRCm39)	Q96K	probably benign	Het
Pramel14	T	C	4: 143,718,192 (GRCm39)	Y417C	probably damaging	Het
Rln1	A	T	19: 29,311,962 (GRCm39)	F12Y	possibly damaging	Het
Setd1a	A	G	7: 127,384,903 (GRCm39)	N585D	possibly damaging	Het
Siah1b	G	A	X: 162,854,688 (GRCm39)	P131S	probably damaging	Het
Smarcal1	T	C	1: 72,650,637 (GRCm39)		probably benign	Het
Snx19	G	T	9: 30,339,895 (GRCm39)	L344F	probably benign	Het
Srfbp1	T	C	18: 52,621,403 (GRCm39)	C155R	probably benign	Het
Sspo	C	T	6: 48,464,287 (GRCm39)	R3914C	probably damaging	Het
Tacc2	G	A	7: 130,225,271 (GRCm39)	S652N	probably damaging	Het
Tmub1	A	C	5: 24,651,068 (GRCm39)	L197R	probably damaging	Het
Tnc	T	C	4: 63,926,066 (GRCm39)	T905A	possibly damaging	Het
Tnfrsf21	G	T	17: 43,398,733 (GRCm39)	R613L	possibly damaging	Het
Ugt3a1	A	T	15: 9,351,283 (GRCm39)	E97D	probably benign	Het
Zfp1010	T	G	2: 176,956,889 (GRCm39)	Q203P	possibly damaging	Het
Zfp568	G	T	7: 29,721,721 (GRCm39)	C221F	probably benign	Het
Zfp91	C	A	19: 12,755,385 (GRCm39)	E217*	probably null	Het
Zswim3	T	A	2: 164,662,563 (GRCm39)	C348S	probably benign	Het

Other mutations in Vmn2r25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Vmn2r25	APN	6	123,830,130 (GRCm39)	missense	probably benign	0.25
IGL01781:Vmn2r25	APN	6	123,816,324 (GRCm39)	missense	possibly damaging	0.48
IGL01843:Vmn2r25	APN	6	123,829,962 (GRCm39)	missense	possibly damaging	0.67
IGL02023:Vmn2r25	APN	6	123,816,388 (GRCm39)	missense	probably damaging	0.96
IGL02502:Vmn2r25	APN	6	123,816,392 (GRCm39)	missense	probably damaging	0.96
IGL02709:Vmn2r25	APN	6	123,816,723 (GRCm39)	missense	possibly damaging	0.50
IGL03053:Vmn2r25	APN	6	123,800,077 (GRCm39)	missense	probably damaging	1.00
PIT4468001:Vmn2r25	UTSW	6	123,816,557 (GRCm39)	missense	probably benign	0.00
PIT4812001:Vmn2r25	UTSW	6	123,800,447 (GRCm39)	missense	probably damaging	1.00
R0054:Vmn2r25	UTSW	6	123,829,984 (GRCm39)	missense	probably benign	0.00
R0312:Vmn2r25	UTSW	6	123,805,539 (GRCm39)	splice site	probably benign
R0366:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R0390:Vmn2r25	UTSW	6	123,800,140 (GRCm39)	missense	probably damaging	1.00
R0466:Vmn2r25	UTSW	6	123,829,008 (GRCm39)	missense	probably benign	0.16
R0541:Vmn2r25	UTSW	6	123,816,786 (GRCm39)	missense	probably damaging	0.97
R0612:Vmn2r25	UTSW	6	123,816,481 (GRCm39)	missense	probably damaging	1.00
R0865:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R1219:Vmn2r25	UTSW	6	123,816,282 (GRCm39)	missense	probably benign	0.00
R1240:Vmn2r25	UTSW	6	123,828,864 (GRCm39)	missense	probably damaging	0.98
R1701:Vmn2r25	UTSW	6	123,828,754 (GRCm39)	splice site	probably null
R1780:Vmn2r25	UTSW	6	123,805,424 (GRCm39)	missense	probably damaging	1.00
R1809:Vmn2r25	UTSW	6	123,802,337 (GRCm39)	missense	probably benign	0.00
R1833:Vmn2r25	UTSW	6	123,816,643 (GRCm39)	missense	probably benign	0.01
R1964:Vmn2r25	UTSW	6	123,800,254 (GRCm39)	missense	possibly damaging	0.94
R2154:Vmn2r25	UTSW	6	123,816,805 (GRCm39)	missense	probably benign	0.01
R2164:Vmn2r25	UTSW	6	123,816,518 (GRCm39)	missense	possibly damaging	0.96
R3799:Vmn2r25	UTSW	6	123,830,143 (GRCm39)	missense	probably benign	0.12
R3836:Vmn2r25	UTSW	6	123,830,044 (GRCm39)	missense	probably damaging	1.00
R3946:Vmn2r25	UTSW	6	123,817,057 (GRCm39)	missense	probably damaging	0.97
R4282:Vmn2r25	UTSW	6	123,800,606 (GRCm39)	missense	probably damaging	1.00
R4367:Vmn2r25	UTSW	6	123,805,496 (GRCm39)	missense	probably damaging	1.00
R4488:Vmn2r25	UTSW	6	123,799,819 (GRCm39)	missense	probably damaging	1.00
R4580:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.46
R4631:Vmn2r25	UTSW	6	123,829,962 (GRCm39)	missense	possibly damaging	0.94
R4765:Vmn2r25	UTSW	6	123,800,182 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r25	UTSW	6	123,805,406 (GRCm39)	missense	probably benign
R5207:Vmn2r25	UTSW	6	123,817,062 (GRCm39)	missense	probably damaging	1.00
R5254:Vmn2r25	UTSW	6	123,802,277 (GRCm39)	missense	probably damaging	1.00
R5444:Vmn2r25	UTSW	6	123,805,451 (GRCm39)	missense	probably benign	0.00
R5586:Vmn2r25	UTSW	6	123,802,255 (GRCm39)	missense	probably damaging	1.00
R5607:Vmn2r25	UTSW	6	123,805,318 (GRCm39)	missense	possibly damaging	0.49
R5985:Vmn2r25	UTSW	6	123,800,587 (GRCm39)	missense	probably benign
R6046:Vmn2r25	UTSW	6	123,799,876 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r25	UTSW	6	123,799,900 (GRCm39)	missense	possibly damaging	0.69
R6569:Vmn2r25	UTSW	6	123,828,941 (GRCm39)	missense	probably benign	0.01
R6826:Vmn2r25	UTSW	6	123,800,071 (GRCm39)	missense	probably damaging	1.00
R7054:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R7120:Vmn2r25	UTSW	6	123,805,394 (GRCm39)	missense	possibly damaging	0.51
R7177:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7287:Vmn2r25	UTSW	6	123,829,040 (GRCm39)	missense	possibly damaging	0.49
R7397:Vmn2r25	UTSW	6	123,800,498 (GRCm39)	missense	probably damaging	0.96
R7486:Vmn2r25	UTSW	6	123,800,101 (GRCm39)	missense	probably damaging	1.00
R7699:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7700:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7759:Vmn2r25	UTSW	6	123,800,339 (GRCm39)	missense	probably damaging	0.99
R7802:Vmn2r25	UTSW	6	123,828,791 (GRCm39)	missense	possibly damaging	0.88
R7850:Vmn2r25	UTSW	6	123,805,431 (GRCm39)	missense	probably damaging	1.00
R8064:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R8170:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R8340:Vmn2r25	UTSW	6	123,829,972 (GRCm39)	missense	probably benign	0.01
R8346:Vmn2r25	UTSW	6	123,802,350 (GRCm39)	missense	probably benign	0.00
R8395:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.81
R8889:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R9094:Vmn2r25	UTSW	6	123,805,391 (GRCm39)	missense	probably benign	0.44
R9204:Vmn2r25	UTSW	6	123,830,092 (GRCm39)	missense	probably benign	0.00
R9253:Vmn2r25	UTSW	6	123,816,960 (GRCm39)	missense	probably damaging	0.98
R9392:Vmn2r25	UTSW	6	123,816,937 (GRCm39)	missense	probably benign
R9520:Vmn2r25	UTSW	6	123,830,066 (GRCm39)	nonsense	probably null
R9525:Vmn2r25	UTSW	6	123,800,164 (GRCm39)	missense	probably damaging	1.00
R9622:Vmn2r25	UTSW	6	123,816,579 (GRCm39)	missense	probably damaging	0.99
X0020:Vmn2r25	UTSW	6	123,816,359 (GRCm39)	missense	possibly damaging	0.95
Z1176:Vmn2r25	UTSW	6	123,799,856 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAATCCCAGAATTTCATCCATGTG -3'
(R):5'- GCAGCTCTGTATCAGGGAATTG -3'

Sequencing Primer
(F):5'- CCCAGAATTTCATCCATGTGAAATG -3'
(R):5'- GTATCAGGGAATTGTCCAACTGATG -3'

Posted On

2015-07-21