Mutagenetix > Incidental Mutation

Incidental Mutation 'R4457:Or51g2'

329951

Institutional Source

Beutler Lab

Gene Symbol

Or51g2

Ensembl Gene

ENSMUSG00000043354

Gene Name

olfactory receptor family 51 subfamily G member 2

Synonyms

Olfr577, GA_x6K02T2PBJ9-5685322-5684384, MOR7-2

MMRRC Submission

041717-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R4457 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

102622259-102623197 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 102622734 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Asparagine at position 155 (S155N)

Ref Sequence

ENSEMBL: ENSMUSP00000150712 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051505] [ENSMUST00000185326] [ENSMUST00000214080] [ENSMUST00000215237]

AlphaFold

Q8VH11

Predicted Effect

probably damaging
Transcript: ENSMUST00000051505
AA Change: S155N

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000059586
Gene: ENSMUSG00000043354
AA Change: S155N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	312	3.3e-140	PFAM
Pfam:7TM_GPCR_Srsx	38	310	1.2e-6	PFAM
Pfam:7tm_1	44	295	7.5e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000185326

SMART Domains

Protein: ENSMUSP00000142459
Gene: ENSMUSG00000073962

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	35	300	9.7e-12	PFAM
Pfam:7tm_1	41	291	1.8e-29	PFAM
Pfam:7tm_4	140	284	2.6e-28	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000214080
AA Change: S155N

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215237
AA Change: S155N

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Meta Mutation Damage Score

0.5366

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

97% (62/64)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008E08Rik	A	G	16: 90,351,260 (GRCm39)		noncoding transcript	Het
1700025G04Rik	T	C	1: 151,796,805 (GRCm39)	R87G	probably damaging	Het
9030619P08Rik	T	A	15: 75,303,249 (GRCm39)		noncoding transcript	Het
Akap13	A	G	7: 75,389,213 (GRCm39)	D2377G	probably damaging	Het
Arhgef5	T	C	6: 43,251,027 (GRCm39)	S593P	probably damaging	Het
Atp4a	G	A	7: 30,419,650 (GRCm39)	R671Q	probably benign	Het
Cdkn2d	C	G	9: 21,202,185 (GRCm39)	V21L	probably benign	Het
Cfap73	T	C	5: 120,768,215 (GRCm39)	K181R	possibly damaging	Het
Chn1	A	G	2: 73,443,427 (GRCm39)	I383T	probably damaging	Het
Cmtr2	A	G	8: 110,948,884 (GRCm39)	D398G	probably benign	Het
Dnah12	T	C	14: 26,537,464 (GRCm39)	Y2238H	probably damaging	Het
Dnah7c	A	G	1: 46,779,781 (GRCm39)	N3161S	probably damaging	Het
Dnah8	C	A	17: 31,032,125 (GRCm39)	H4148Q	probably benign	Het
Ehbp1l1	T	C	19: 5,766,321 (GRCm39)	S397G	possibly damaging	Het
Eya4	A	T	10: 22,992,566 (GRCm39)	S462R	probably damaging	Het
Fam227b	T	A	2: 125,988,188 (GRCm39)		probably benign	Het
Frrs1	G	A	3: 116,690,377 (GRCm39)	V7I	probably benign	Het
Fsip2	G	T	2: 82,821,120 (GRCm39)	A5618S	possibly damaging	Het
Gja10	A	T	4: 32,601,073 (GRCm39)	M437K	probably benign	Het
Gm2840	T	G	5: 96,322,187 (GRCm39)		noncoding transcript	Het
Gm4956	A	G	1: 21,368,319 (GRCm39)		noncoding transcript	Het
Gria4	A	G	9: 4,427,074 (GRCm39)	W789R	probably damaging	Het
Hoxb5	A	G	11: 96,194,546 (GRCm39)	D36G	probably damaging	Het
Hps5	A	T	7: 46,433,037 (GRCm39)	C228S	probably benign	Het
Hspa4	A	T	11: 53,171,395 (GRCm39)	C270S	probably damaging	Het
Htra4	C	A	8: 25,528,674 (GRCm39)	A73S	possibly damaging	Het
Ikzf2	T	C	1: 69,723,347 (GRCm39)		probably benign	Het
Ivl	G	A	3: 92,479,673 (GRCm39)	H131Y	probably benign	Het
Kat6a	A	G	8: 23,422,129 (GRCm39)		probably null	Het
Lamp3	T	A	16: 19,492,279 (GRCm39)	M322L	probably benign	Het
Letmd1	T	C	15: 100,373,011 (GRCm39)	V37A	possibly damaging	Het
Mslnl	G	A	17: 25,961,908 (GRCm39)	V128M	probably damaging	Het
Myh1	G	A	11: 67,111,441 (GRCm39)	G1627R	probably benign	Het
Myo9b	A	G	8: 71,743,643 (GRCm39)	I235V	probably damaging	Het
Ndor1	A	G	2: 25,138,128 (GRCm39)		probably null	Het
Ndufa12	A	T	10: 94,056,680 (GRCm39)	K136M	probably damaging	Het
Or2t49	A	G	11: 58,392,944 (GRCm39)	L146P	probably damaging	Het
Or4a78	A	T	2: 89,497,427 (GRCm39)	S268T	probably benign	Het
Or5d44	A	G	2: 88,141,173 (GRCm39)		probably benign	Het
Pcdha2	A	G	18: 37,073,599 (GRCm39)	D410G	probably damaging	Het
Pcyox1l	T	A	18: 61,830,939 (GRCm39)	N311I	probably benign	Het
Pif1	A	G	9: 65,495,058 (GRCm39)		probably benign	Het
Pkp1	CTCTTCTT	CTCTT	1: 135,803,362 (GRCm39)		probably null	Het
Pogz	G	A	3: 94,763,374 (GRCm39)	V49I	probably benign	Het
Rab36	G	A	10: 74,880,328 (GRCm39)	V63I	probably damaging	Het
Rnf4	A	G	5: 34,508,705 (GRCm39)	Y189C	probably benign	Het
Rpl31-ps17	C	T	12: 54,748,397 (GRCm39)		noncoding transcript	Het
Sgms2	A	C	3: 131,118,665 (GRCm39)	Y273D	probably damaging	Het
Slc25a10	G	A	11: 120,387,915 (GRCm39)	V203I	probably benign	Het
Slc4a2	T	A	5: 24,639,328 (GRCm39)		probably benign	Het
Tbc1d13	A	G	2: 30,025,450 (GRCm39)		probably benign	Het
Tdrd7	A	G	4: 46,007,526 (GRCm39)	N526S	probably benign	Het
Tet2	C	T	3: 133,191,324 (GRCm39)	D1037N	possibly damaging	Het
Thrb	T	A	14: 18,011,187 (GRCm38)	W188R	probably damaging	Het
Ttn	T	C	2: 76,777,257 (GRCm39)	M1382V	probably benign	Het
Usp9y	T	A	Y: 1,394,078 (GRCm39)	I551L	possibly damaging	Het
Vwde	T	C	6: 13,196,100 (GRCm39)	I308M	probably damaging	Het
Zan	T	C	5: 137,409,778 (GRCm39)	I3488V	unknown	Het
Zgrf1	A	T	3: 127,389,578 (GRCm39)	I375F	probably damaging	Het
Zic4	A	G	9: 91,261,315 (GRCm39)	K183R	probably damaging	Het

Other mutations in Or51g2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02246:Or51g2	APN	7	102,622,951 (GRCm39)	missense	possibly damaging	0.62
IGL03111:Or51g2	APN	7	102,622,738 (GRCm39)	missense	probably damaging	1.00
R1529:Or51g2	UTSW	7	102,623,086 (GRCm39)	missense	probably damaging	1.00
R1753:Or51g2	UTSW	7	102,622,263 (GRCm39)	missense	probably benign
R3005:Or51g2	UTSW	7	102,622,465 (GRCm39)	missense	possibly damaging	0.56
R4675:Or51g2	UTSW	7	102,623,013 (GRCm39)	missense	probably damaging	0.99
R4808:Or51g2	UTSW	7	102,623,118 (GRCm39)	missense	probably damaging	0.99
R4891:Or51g2	UTSW	7	102,622,759 (GRCm39)	missense	probably benign	0.12
R4917:Or51g2	UTSW	7	102,622,614 (GRCm39)	missense	possibly damaging	0.93
R4918:Or51g2	UTSW	7	102,622,614 (GRCm39)	missense	possibly damaging	0.93
R5328:Or51g2	UTSW	7	102,623,175 (GRCm39)	missense	possibly damaging	0.46
R6375:Or51g2	UTSW	7	102,622,960 (GRCm39)	missense	probably damaging	1.00
R6683:Or51g2	UTSW	7	102,622,920 (GRCm39)	missense	probably benign	0.05
R6958:Or51g2	UTSW	7	102,623,091 (GRCm39)	missense	possibly damaging	0.67
R7022:Or51g2	UTSW	7	102,623,175 (GRCm39)	missense	possibly damaging	0.46
R7429:Or51g2	UTSW	7	102,622,969 (GRCm39)	missense	probably damaging	1.00
R7430:Or51g2	UTSW	7	102,622,969 (GRCm39)	missense	probably damaging	1.00
R7490:Or51g2	UTSW	7	102,623,017 (GRCm39)	missense	probably damaging	1.00
R7808:Or51g2	UTSW	7	102,622,317 (GRCm39)	missense	possibly damaging	0.56
R8169:Or51g2	UTSW	7	102,622,545 (GRCm39)	missense	probably damaging	0.99
R8544:Or51g2	UTSW	7	102,622,938 (GRCm39)	missense	probably damaging	1.00
R9027:Or51g2	UTSW	7	102,622,560 (GRCm39)	missense	probably damaging	1.00
R9265:Or51g2	UTSW	7	102,623,112 (GRCm39)	nonsense	probably null
X0027:Or51g2	UTSW	7	102,622,893 (GRCm39)	missense	probably benign	0.05
Z1176:Or51g2	UTSW	7	102,622,516 (GRCm39)	missense	not run
Z1177:Or51g2	UTSW	7	102,622,516 (GRCm39)	missense	not run

Predicted Primers

PCR Primer
(F):5'- AATGGACAATACGGTGCGC -3'
(R):5'- TGATGCTTGCTTTACCCAGC -3'

Sequencing Primer
(F):5'- GGATGAGCAAAGAGTCCACTCC -3'
(R):5'- AGCTCTTTTTCATCCACTGCTTG -3'

Posted On

2015-07-21