Mutagenetix > Incidental Mutation

Incidental Mutation 'R4472:Wls'

330398

Institutional Source

Beutler Lab

Gene Symbol

Wls

Ensembl Gene

ENSMUSG00000028173

Gene Name

wntless WNT ligand secretion mediator

Synonyms

5031439A09Rik, Gpr177

MMRRC Submission

041729-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4472 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

159545309-159644300 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 159603020 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 144 (M144I)

Ref Sequence

ENSEMBL: ENSMUSP00000143475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068952] [ENSMUST00000198878] [ENSMUST00000200191]

AlphaFold

Q6DID7

Predicted Effect

probably benign
Transcript: ENSMUST00000068952
AA Change: M144I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000067898
Gene: ENSMUSG00000028173
AA Change: M144I

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	178	496	3.7e-97	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197328

Predicted Effect

probably benign
Transcript: ENSMUST00000198878
AA Change: M144I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000143475
Gene: ENSMUSG00000028173
AA Change: M144I

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	177	497	2.8e-94	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000200191

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200571

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

97% (66/68)

MGI Phenotype

PHENOTYPE: Mice homozygous for null alleles exhibit impaired body axis and triploblastic development dying prior to E10.5. Mice homozygous for a floxed allele activated in keratinocytes exhibit a psoriasiform dermatitis-like phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001J11Rik	A	T	9: 39,961,994 (GRCm39)		noncoding transcript	Het
Accsl	T	A	2: 93,694,336 (GRCm39)		probably null	Het
Accsl	G	T	2: 93,694,337 (GRCm39)		probably null	Het
Adcy1	G	A	11: 7,080,369 (GRCm39)	V371M	probably damaging	Het
Adgrb2	C	T	4: 129,902,146 (GRCm39)	A509V	probably benign	Het
Adgrg6	T	A	10: 14,312,525 (GRCm39)	Q754L	probably damaging	Het
Agap2	T	C	10: 126,927,082 (GRCm39)	I1008T	probably damaging	Het
Aph1c	T	G	9: 66,735,051 (GRCm39)	H150P	probably damaging	Het
Atcay	T	C	10: 81,048,361 (GRCm39)	R242G	possibly damaging	Het
Atg2a	G	A	19: 6,308,985 (GRCm39)	V1724M	probably damaging	Het
Bbs12	T	C	3: 37,373,369 (GRCm39)	V54A	possibly damaging	Het
Card14	A	G	11: 119,224,784 (GRCm39)	M604V	possibly damaging	Het
Cd28	A	T	1: 60,802,393 (GRCm39)	H104L	probably benign	Het
Cntn5	A	T	9: 10,048,776 (GRCm39)	D262E	probably damaging	Het
Col6a3	A	G	1: 90,749,736 (GRCm39)	V366A	probably benign	Het
Csnk1d	G	A	11: 120,855,800 (GRCm39)		probably benign	Het
Dcaf11	T	A	14: 55,803,063 (GRCm39)		probably benign	Het
Dpy19l4	G	A	4: 11,304,053 (GRCm39)	T119M	possibly damaging	Het
Eif3f	T	C	7: 108,540,153 (GRCm39)	V316A	possibly damaging	Het
Fbrsl1	G	A	5: 110,526,932 (GRCm39)		probably benign	Het
Fbxo10	C	T	4: 45,043,693 (GRCm39)	R710H	probably damaging	Het
Fbxw9	G	A	8: 85,786,829 (GRCm39)	D25N	probably damaging	Het
Gm14295	C	T	2: 176,501,386 (GRCm39)	T292I	possibly damaging	Het
Gm4884	C	G	7: 40,692,687 (GRCm39)	Q219E	probably benign	Het
Gpr150	A	G	13: 76,204,273 (GRCm39)	V224A	probably benign	Het
Hps1	A	T	19: 42,750,935 (GRCm39)	I355N	probably damaging	Het
Lmbr1l	A	G	15: 98,804,178 (GRCm39)	S374P	probably benign	Het
Macf1	A	T	4: 123,289,782 (GRCm39)	N5458K	probably damaging	Het
Man1b1	C	G	2: 25,222,867 (GRCm39)		probably benign	Het
Morc3	A	G	16: 93,671,645 (GRCm39)		probably null	Het
Mrgpra4	T	C	7: 47,631,539 (GRCm39)	T21A	probably benign	Het
Myo1a	G	T	10: 127,546,327 (GRCm39)	V277L	probably benign	Het
Nkain4	C	G	2: 180,596,415 (GRCm39)	M1I	probably null	Het
Nktr	T	C	9: 121,577,962 (GRCm39)		probably benign	Het
Nrxn3	T	C	12: 90,171,515 (GRCm39)	S276P	probably damaging	Het
Oas2	A	T	5: 120,879,220 (GRCm39)	D373E	possibly damaging	Het
Or8g37	T	A	9: 39,731,870 (GRCm39)	*312R	probably null	Het
Oser1	T	A	2: 163,257,500 (GRCm39)	E11V	probably null	Het
Oser1	C	T	2: 163,257,501 (GRCm39)	E11K	probably damaging	Het
Pacsin3	A	G	2: 91,093,288 (GRCm39)		probably null	Het
Pcdh20	T	C	14: 88,706,434 (GRCm39)	S289G	probably benign	Het
Phlpp1	A	G	1: 106,314,176 (GRCm39)	D1183G	probably damaging	Het
Pign	T	C	1: 105,575,945 (GRCm39)	K232E	probably benign	Het
Polk	A	G	13: 96,630,413 (GRCm39)	S383P	probably damaging	Het
Prom2	C	A	2: 127,382,111 (GRCm39)	R101L	probably benign	Het
Rest	T	C	5: 77,429,027 (GRCm39)	V482A	probably benign	Het
Rexo1	A	T	10: 80,378,492 (GRCm39)	S476T	probably damaging	Het
Rnf31	T	A	14: 55,840,777 (GRCm39)	Y1015N	probably damaging	Het
Spata3	T	C	1: 85,954,152 (GRCm39)	Y305H	probably benign	Het
Sv2a	G	A	3: 96,099,810 (GRCm39)	V587M	probably benign	Het
Synj1	A	G	16: 90,766,069 (GRCm39)		probably null	Het
Syt17	A	G	7: 118,036,040 (GRCm39)		probably null	Het
Taf2	T	C	15: 54,922,276 (GRCm39)	D337G	possibly damaging	Het
Tcaf1	A	C	6: 42,656,248 (GRCm39)	S243A	probably benign	Het
Tmem9	A	G	1: 135,955,234 (GRCm39)	T123A	probably benign	Het
Trav4-4-dv10	T	C	14: 53,921,187 (GRCm39)		probably benign	Het
Trbv15	G	A	6: 41,118,493 (GRCm39)	R83Q	probably damaging	Het
Trim27	A	G	13: 21,374,056 (GRCm39)	I268V	probably benign	Het
Tubgcp6	T	A	15: 88,987,857 (GRCm39)	S1031C	probably damaging	Het
Vmn1r89	C	A	7: 12,953,799 (GRCm39)	H110Q	probably benign	Het
Vmn2r30	T	C	7: 7,320,091 (GRCm39)	N545S	probably damaging	Het
Yme1l1	T	C	2: 23,076,344 (GRCm39)		probably null	Het
Zeb2	A	T	2: 44,913,023 (GRCm39)	L56Q	probably damaging	Het

Other mutations in Wls

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01924:Wls	APN	3	159,607,080 (GRCm39)	nonsense	probably null
IGL02065:Wls	APN	3	159,616,993 (GRCm39)	missense	probably damaging	0.99
IGL02073:Wls	APN	3	159,612,890 (GRCm39)	critical splice donor site	probably null
IGL02133:Wls	APN	3	159,603,007 (GRCm39)	missense	probably damaging	1.00
R0374:Wls	UTSW	3	159,603,074 (GRCm39)	nonsense	probably null
R0561:Wls	UTSW	3	159,578,705 (GRCm39)	missense	probably benign	0.32
R1697:Wls	UTSW	3	159,602,995 (GRCm39)	missense	probably benign	0.12
R1791:Wls	UTSW	3	159,617,450 (GRCm39)	missense	probably benign	0.17
R2444:Wls	UTSW	3	159,612,867 (GRCm39)	missense	probably damaging	1.00
R3161:Wls	UTSW	3	159,603,073 (GRCm39)	missense	probably damaging	1.00
R4285:Wls	UTSW	3	159,639,902 (GRCm39)	missense	probably benign
R4468:Wls	UTSW	3	159,578,564 (GRCm39)	missense	probably damaging	0.96
R4782:Wls	UTSW	3	159,603,082 (GRCm39)	missense	probably benign	0.03
R4799:Wls	UTSW	3	159,603,082 (GRCm39)	missense	probably benign	0.03
R4809:Wls	UTSW	3	159,603,082 (GRCm39)	missense	probably benign	0.03
R5006:Wls	UTSW	3	159,617,428 (GRCm39)	missense	possibly damaging	0.68
R5212:Wls	UTSW	3	159,578,645 (GRCm39)	missense	probably benign	0.15
R5434:Wls	UTSW	3	159,639,976 (GRCm39)	missense	probably damaging	0.97
R5694:Wls	UTSW	3	159,545,624 (GRCm39)	missense	probably benign	0.01
R6315:Wls	UTSW	3	159,640,007 (GRCm39)	critical splice donor site	probably null
R7069:Wls	UTSW	3	159,639,965 (GRCm39)	missense	probably damaging	1.00
R7243:Wls	UTSW	3	159,615,402 (GRCm39)	missense	possibly damaging	0.49
R7529:Wls	UTSW	3	159,578,644 (GRCm39)	missense	probably benign	0.43
R7697:Wls	UTSW	3	159,616,955 (GRCm39)	missense	probably benign	0.21
R7842:Wls	UTSW	3	159,578,816 (GRCm39)	missense	probably benign	0.09
R8136:Wls	UTSW	3	159,578,761 (GRCm39)	missense	probably damaging	1.00
R8536:Wls	UTSW	3	159,578,748 (GRCm39)	missense	probably damaging	0.96
R8816:Wls	UTSW	3	159,639,928 (GRCm39)	missense	possibly damaging	0.89
R9074:Wls	UTSW	3	159,615,403 (GRCm39)	missense	possibly damaging	0.86

Predicted Primers

PCR Primer
(F):5'- TTGACTGCAAAGGGTAGGC -3'
(R):5'- TTCAGTGGAAAGTCTAGCCTTTC -3'

Sequencing Primer
(F):5'- TAGGCTATGGAAGGAGATCTAACATC -3'
(R):5'- GAAAGTCTAGCCTTTCCTTCACTC -3'

Posted On

2015-07-21