Mutagenetix > Incidental Mutation

Incidental Mutation 'R4474:Vmn2r95'

330501

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r95

Ensembl Gene

ENSMUSG00000091631

Gene Name

vomeronasal 2, receptor 95

Synonyms

MMRRC Submission

041731-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R4474 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

18644366-18672586 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 18672507 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 820 (L820P)

Ref Sequence

ENSEMBL: ENSMUSP00000156117 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166327] [ENSMUST00000232090] [ENSMUST00000232464]

AlphaFold

A0A338P6T0

Predicted Effect

probably benign
Transcript: ENSMUST00000166327

SMART Domains

Protein: ENSMUSP00000126106
Gene: ENSMUSG00000091631

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	82	462	1.8e-35	PFAM
Pfam:NCD3G	509	562	3.2e-20	PFAM
Pfam:7tm_3	594	830	3.2e-51	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000232090
AA Change: L820P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000232464
AA Change: L748P

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.9%
20x: 94.2%

Validation Efficiency

95% (36/38)

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	A	T	11: 110,124,598 (GRCm39)	I397K	possibly damaging	Het
Alpk1	T	C	3: 127,473,667 (GRCm39)	T779A	probably damaging	Het
Cdh23	G	T	10: 60,146,865 (GRCm39)	A2795D	probably damaging	Het
Cdk19	G	A	10: 40,345,952 (GRCm39)		probably null	Het
Cep104	T	A	4: 154,073,693 (GRCm39)	M476K	possibly damaging	Het
Csf1	T	C	3: 107,661,172 (GRCm39)	D115G	probably damaging	Het
Dclre1b	C	A	3: 103,714,559 (GRCm39)		probably benign	Het
Fbp1	A	G	13: 63,023,075 (GRCm39)	L74P	probably damaging	Het
Hivep2	C	A	10: 14,004,713 (GRCm39)	T437K	probably benign	Het
Hydin	G	T	8: 111,290,497 (GRCm39)	V3319L	probably benign	Het
Kcna4	A	G	2: 107,126,352 (GRCm39)	N362S	probably benign	Het
Kif13a	C	T	13: 46,967,631 (GRCm39)		probably null	Het
Lingo2	T	C	4: 35,708,810 (GRCm39)	E390G	probably benign	Het
Mdh1	T	G	11: 21,516,624 (GRCm39)	D33A	possibly damaging	Het
Mov10	C	T	3: 104,725,781 (GRCm39)	G21D	probably damaging	Het
Muc21	G	T	17: 35,931,496 (GRCm39)		probably benign	Het
Or4f56	G	A	2: 111,703,784 (GRCm39)	R139W	possibly damaging	Het
Or7g32	T	A	9: 19,408,173 (GRCm39)	L43Q	probably damaging	Het
Parn	A	G	16: 13,482,549 (GRCm39)	S100P	probably benign	Het
Pik3c2g	T	A	6: 139,610,749 (GRCm39)	Y332N	probably damaging	Het
Rab22a	C	T	2: 173,537,056 (GRCm39)	T85M	probably damaging	Het
Rims2	A	G	15: 39,325,956 (GRCm39)	S763G	probably damaging	Het
Sarm1	T	A	11: 78,387,927 (GRCm39)	I120L	probably benign	Het
Siah1b	G	A	X: 162,854,688 (GRCm39)	P131S	probably damaging	Het
Snx31	A	G	15: 36,546,256 (GRCm39)		probably benign	Het
Tmem150a	C	T	6: 72,334,035 (GRCm39)	S39F	probably benign	Het
Tsc2	T	A	17: 24,816,238 (GRCm39)	H1605L	probably damaging	Het
Ugt1a10	TAAAAAAAAA	TAAAAAAA	1: 88,143,650 (GRCm39)		probably benign	Het
Zfp36l3	T	C	X: 52,777,924 (GRCm39)	N97D	possibly damaging	Het

Other mutations in Vmn2r95

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01024:Vmn2r95	APN	17	18,672,590 (GRCm39)	utr 3 prime	probably benign
IGL01479:Vmn2r95	APN	17	18,664,124 (GRCm39)	missense	probably damaging	1.00
IGL01890:Vmn2r95	APN	17	18,671,737 (GRCm39)	missense	probably damaging	1.00
IGL01986:Vmn2r95	APN	17	18,660,473 (GRCm39)	missense	probably benign	0.06
IGL02113:Vmn2r95	APN	17	18,660,169 (GRCm39)	missense	possibly damaging	0.47
IGL02154:Vmn2r95	APN	17	18,672,248 (GRCm39)	missense	probably benign	0.16
IGL02190:Vmn2r95	APN	17	18,672,038 (GRCm39)	missense	probably benign	0.00
IGL02412:Vmn2r95	APN	17	18,660,218 (GRCm39)	missense	probably damaging	1.00
IGL02550:Vmn2r95	APN	17	18,671,994 (GRCm39)	missense	probably damaging	1.00
IGL02679:Vmn2r95	APN	17	18,664,116 (GRCm39)	missense	probably damaging	1.00
IGL02691:Vmn2r95	APN	17	18,672,120 (GRCm39)	missense	probably benign	0.07
IGL02990:Vmn2r95	APN	17	18,672,298 (GRCm39)	nonsense	probably null
IGL03032:Vmn2r95	APN	17	18,672,575 (GRCm39)	missense	probably benign	0.00
R0416:Vmn2r95	UTSW	17	18,661,664 (GRCm39)	missense	probably damaging	1.00
R0448:Vmn2r95	UTSW	17	18,672,005 (GRCm39)	missense	possibly damaging	0.92
R0514:Vmn2r95	UTSW	17	18,671,844 (GRCm39)	missense	probably benign
R0519:Vmn2r95	UTSW	17	18,659,765 (GRCm39)	missense	probably damaging	1.00
R0539:Vmn2r95	UTSW	17	18,672,362 (GRCm39)	missense	probably damaging	1.00
R1501:Vmn2r95	UTSW	17	18,660,118 (GRCm39)	missense	probably damaging	0.99
R1598:Vmn2r95	UTSW	17	18,672,575 (GRCm39)	missense	probably benign	0.03
R1613:Vmn2r95	UTSW	17	18,660,901 (GRCm39)	splice site	probably benign
R1861:Vmn2r95	UTSW	17	18,672,530 (GRCm39)	missense	probably damaging	1.00
R1921:Vmn2r95	UTSW	17	18,644,575 (GRCm39)	missense	probably benign	0.11
R1986:Vmn2r95	UTSW	17	18,671,805 (GRCm39)	missense	probably benign
R2031:Vmn2r95	UTSW	17	18,659,717 (GRCm39)	missense	possibly damaging	0.94
R2040:Vmn2r95	UTSW	17	18,661,561 (GRCm39)	missense	probably damaging	1.00
R3608:Vmn2r95	UTSW	17	18,660,235 (GRCm39)	missense	possibly damaging	0.47
R3727:Vmn2r95	UTSW	17	18,661,744 (GRCm39)	nonsense	probably null
R3953:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3955:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3957:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R4672:Vmn2r95	UTSW	17	18,672,413 (GRCm39)	missense	probably damaging	1.00
R4850:Vmn2r95	UTSW	17	18,671,915 (GRCm39)	missense	probably damaging	1.00
R5054:Vmn2r95	UTSW	17	18,671,708 (GRCm39)	missense	possibly damaging	0.63
R5178:Vmn2r95	UTSW	17	18,660,337 (GRCm39)	missense	probably benign	0.01
R5980:Vmn2r95	UTSW	17	18,661,624 (GRCm39)	missense	probably benign
R6183:Vmn2r95	UTSW	17	18,664,192 (GRCm39)	missense	probably damaging	0.99
R6276:Vmn2r95	UTSW	17	18,671,732 (GRCm39)	missense	possibly damaging	0.96
R6651:Vmn2r95	UTSW	17	18,660,622 (GRCm39)	missense	probably damaging	1.00
R6682:Vmn2r95	UTSW	17	18,660,489 (GRCm39)	missense	probably damaging	1.00
R6797:Vmn2r95	UTSW	17	18,672,551 (GRCm39)	utr 3 prime	probably benign
R6799:Vmn2r95	UTSW	17	18,659,555 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,182 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,181 (GRCm39)	missense	probably damaging	1.00
R6982:Vmn2r95	UTSW	17	18,672,323 (GRCm39)	missense	probably damaging	1.00
R7203:Vmn2r95	UTSW	17	18,661,577 (GRCm39)	missense	probably benign	0.01
R7226:Vmn2r95	UTSW	17	18,672,245 (GRCm39)	missense	possibly damaging	0.90
R7240:Vmn2r95	UTSW	17	18,672,225 (GRCm39)	missense	probably benign	0.15
R7383:Vmn2r95	UTSW	17	18,660,734 (GRCm39)	missense	probably benign	0.06
R7614:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R7755:Vmn2r95	UTSW	17	18,644,367 (GRCm39)	start codon destroyed	probably null	0.99
R7942:Vmn2r95	UTSW	17	18,660,529 (GRCm39)	missense	possibly damaging	0.74
R8355:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8455:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8478:Vmn2r95	UTSW	17	18,672,544 (GRCm39)	missense	probably damaging	1.00
R8547:Vmn2r95	UTSW	17	18,664,161 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r95	UTSW	17	18,661,738 (GRCm39)	missense	probably damaging	0.98
R8788:Vmn2r95	UTSW	17	18,671,790 (GRCm39)	missense	probably benign	0.09
R8852:Vmn2r95	UTSW	17	18,664,113 (GRCm39)	missense	possibly damaging	0.95
R9098:Vmn2r95	UTSW	17	18,660,167 (GRCm39)	missense	possibly damaging	0.88
R9202:Vmn2r95	UTSW	17	18,644,394 (GRCm39)	missense	probably benign	0.00
R9244:Vmn2r95	UTSW	17	18,672,189 (GRCm39)	missense	possibly damaging	0.91
R9546:Vmn2r95	UTSW	17	18,661,721 (GRCm39)	missense	probably benign	0.01
R9665:Vmn2r95	UTSW	17	18,660,607 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r95	UTSW	17	18,660,663 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTTATGCCATGGCCTTCTTG -3'
(R):5'- AGGTCTTAGACTCAGAGCAGC -3'

Sequencing Primer
(F):5'- CTTGTCTAGGAATTTGCCAGATAC -3'
(R):5'- CTTAGACTCAGAGCAGCTGTATAAC -3'

Posted On

2015-07-21