Mutagenetix > Incidental Mutation

Incidental Mutation 'R4491:Or10ag60'

330705

Institutional Source

Beutler Lab

Gene Symbol

Or10ag60

Ensembl Gene

ENSMUSG00000059205

Gene Name

olfactory receptor family 10 subfamily AG member 60

Synonyms

GA_x6K02T2Q125-49112575-49113519, Olfr1130, MOR264-4

MMRRC Submission

041747-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.186) question?

Stock #

R4491 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

87436556-87438678 bp(+) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

G to A at 87437736 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 1 (M1I)

Ref Sequence

ENSEMBL: ENSMUSP00000150905 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079711] [ENSMUST00000213103] [ENSMUST00000216580]

AlphaFold

A0A1L1SQT2

Predicted Effect

probably null
Transcript: ENSMUST00000079711
AA Change: M1I

PolyPhen 2 Score 0.752 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000078649
Gene: ENSMUSG00000068814
AA Change: M1I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	314	3.4e-52	PFAM
Pfam:7tm_1	47	296	1.3e-19	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213103
AA Change: M1I

PolyPhen 2 Score 0.840 (Sensitivity: 0.84; Specificity: 0.93)

Predicted Effect

probably null
Transcript: ENSMUST00000216580
AA Change: M1I

PolyPhen 2 Score 0.840 (Sensitivity: 0.84; Specificity: 0.93)

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

98% (64/65)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	C	A	5: 64,055,812 (GRCm39)	P183T	probably damaging	Het
Bcan	G	A	3: 87,897,540 (GRCm39)	R682*	probably null	Het
Bzw1	T	A	1: 58,443,418 (GRCm39)	L410Q	probably damaging	Het
Cdh5	A	T	8: 104,839,672 (GRCm39)	I48F	probably damaging	Het
Cdhr5	T	C	7: 140,853,970 (GRCm39)	N173D	possibly damaging	Het
Cfap74	T	C	4: 155,513,628 (GRCm39)	M480T	probably benign	Het
Col6a5	C	T	9: 105,817,211 (GRCm39)	A367T	unknown	Het
Cpsf1	A	C	15: 76,481,922 (GRCm39)	Y1064D	possibly damaging	Het
Defb6	A	T	8: 19,278,090 (GRCm39)	H54L	probably benign	Het
Dmtf1	A	G	5: 9,190,379 (GRCm39)		probably benign	Het
Dnah12	T	C	14: 26,455,758 (GRCm39)	L827S	possibly damaging	Het
Dnai4	T	C	4: 102,923,596 (GRCm39)	E411G	probably benign	Het
Dsc3	T	C	18: 20,134,922 (GRCm39)	T21A	probably benign	Het
Epb41l1	G	T	2: 156,364,088 (GRCm39)	D866Y	probably benign	Het
Epha1	T	C	6: 42,337,600 (GRCm39)	M860V	probably damaging	Het
Far2	T	C	6: 148,074,907 (GRCm39)	L380P	possibly damaging	Het
Fgd5	A	G	6: 91,966,280 (GRCm39)	I680V	possibly damaging	Het
Fnbp4	T	C	2: 90,583,312 (GRCm39)		probably null	Het
Focad	G	A	4: 88,278,142 (GRCm39)		probably null	Het
Gm14180	T	A	11: 99,621,139 (GRCm39)		probably benign	Het
Hbb-bh2	T	C	7: 103,489,622 (GRCm39)	T5A	probably benign	Het
Ighv7-2	A	G	12: 113,876,100 (GRCm39)	F2L	probably benign	Het
Igsf5	A	G	16: 96,165,281 (GRCm39)	T19A	probably benign	Het
Kcnd3	C	T	3: 105,566,082 (GRCm39)	A421V	probably damaging	Het
Klb	A	G	5: 65,533,137 (GRCm39)	N482S	probably benign	Het
Lipo2	A	C	19: 33,699,100 (GRCm39)	L310R	probably damaging	Het
Mc3r	A	G	2: 172,091,123 (GRCm39)	H115R	possibly damaging	Het
Meioc	A	G	11: 102,565,746 (GRCm39)	D398G	possibly damaging	Het
Or56a5	A	T	7: 104,792,983 (GRCm39)	C172*	probably null	Het
Or7a37	T	C	10: 78,806,151 (GRCm39)	S223P	probably damaging	Het
Pds5a	T	C	5: 65,792,780 (GRCm39)	T718A	probably benign	Het
Pdzd2	C	T	15: 12,385,723 (GRCm39)	D1016N	possibly damaging	Het
Pla2g2c	T	C	4: 138,461,719 (GRCm39)		probably null	Het
Plch1	T	C	3: 63,648,160 (GRCm39)	I404V	probably damaging	Het
Pou2af3	A	G	9: 51,181,955 (GRCm39)	F206L	probably benign	Het
Ppp2r5c	A	T	12: 110,546,956 (GRCm39)	D522V	possibly damaging	Het
Prex2	T	A	1: 11,232,487 (GRCm39)	S851R	probably benign	Het
Rapgef1	C	T	2: 29,609,668 (GRCm39)	P702S	possibly damaging	Het
Rasal3	T	A	17: 32,610,359 (GRCm39)	D976V	probably damaging	Het
Rasef	A	C	4: 73,652,740 (GRCm39)	L587R	probably damaging	Het
Rptn	A	T	3: 93,303,818 (GRCm39)	R384*	probably null	Het
Sema6a	G	A	18: 47,439,524 (GRCm39)		probably benign	Het
Sycp2	G	T	2: 178,016,778 (GRCm39)	T608K	probably damaging	Het
Syde1	C	T	10: 78,426,062 (GRCm39)	R35H	probably benign	Het
Taf1	G	T	X: 100,586,665 (GRCm39)	M313I	possibly damaging	Het
Taf15	A	T	11: 83,375,520 (GRCm39)	T31S	probably benign	Het
Tktl2	T	C	8: 66,964,664 (GRCm39)	V74A	probably damaging	Het
Tle4	A	G	19: 14,432,229 (GRCm39)	V489A	probably damaging	Het
Tmem30a	T	C	9: 79,684,567 (GRCm39)	H95R	probably damaging	Het
Trmt9b	G	A	8: 36,972,760 (GRCm39)	C70Y	probably damaging	Het
Vmn1r75	C	A	7: 11,614,909 (GRCm39)	Q214K	probably damaging	Het
Vps13c	C	T	9: 67,817,475 (GRCm39)	T1049M	probably benign	Het
Zfp37	T	C	4: 62,110,365 (GRCm39)	Q274R	probably benign	Het
Zfp648	T	G	1: 154,080,873 (GRCm39)	L344R	probably damaging	Het

Other mutations in Or10ag60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01151:Or10ag60	APN	2	87,438,323 (GRCm39)	missense	probably damaging	1.00
IGL01310:Or10ag60	APN	2	87,437,852 (GRCm39)	missense	possibly damaging	0.68
IGL02558:Or10ag60	APN	2	87,437,903 (GRCm39)	missense	probably benign	0.00
R0323:Or10ag60	UTSW	2	87,437,841 (GRCm39)	missense	probably benign
R0590:Or10ag60	UTSW	2	87,438,338 (GRCm39)	missense	probably damaging	0.98
R0718:Or10ag60	UTSW	2	87,438,271 (GRCm39)	missense	probably benign	0.08
R2445:Or10ag60	UTSW	2	87,438,302 (GRCm39)	missense	probably damaging	0.99
R3408:Or10ag60	UTSW	2	87,438,220 (GRCm39)	missense	probably benign	0.03
R4280:Or10ag60	UTSW	2	87,438,595 (GRCm39)	missense	possibly damaging	0.90
R4928:Or10ag60	UTSW	2	87,438,487 (GRCm39)	missense	probably benign	0.06
R5033:Or10ag60	UTSW	2	87,438,055 (GRCm39)	missense	probably damaging	1.00
R5061:Or10ag60	UTSW	2	87,438,176 (GRCm39)	missense	probably benign	0.00
R5109:Or10ag60	UTSW	2	87,438,319 (GRCm39)	missense	possibly damaging	0.78
R5109:Or10ag60	UTSW	2	87,437,755 (GRCm39)	missense	possibly damaging	0.64
R5772:Or10ag60	UTSW	2	87,438,517 (GRCm39)	missense	probably benign	0.12
R6004:Or10ag60	UTSW	2	87,438,253 (GRCm39)	missense	probably damaging	1.00
R6005:Or10ag60	UTSW	2	87,438,424 (GRCm39)	missense	probably damaging	0.97
R6411:Or10ag60	UTSW	2	87,438,317 (GRCm39)	missense	probably damaging	1.00
R6964:Or10ag60	UTSW	2	87,437,957 (GRCm39)	missense	probably damaging	1.00
R7085:Or10ag60	UTSW	2	87,437,750 (GRCm39)	missense	probably benign	0.00
R7484:Or10ag60	UTSW	2	87,438,281 (GRCm39)	missense	probably damaging	1.00
R8429:Or10ag60	UTSW	2	87,437,868 (GRCm39)	missense	probably benign	0.06
R8471:Or10ag60	UTSW	2	87,437,989 (GRCm39)	missense	probably damaging	0.99
R8485:Or10ag60	UTSW	2	87,438,601 (GRCm39)	missense	probably benign	0.00
R8890:Or10ag60	UTSW	2	87,438,412 (GRCm39)	missense	probably damaging	1.00
R8935:Or10ag60	UTSW	2	87,438,421 (GRCm39)	missense	possibly damaging	0.72
X0060:Or10ag60	UTSW	2	87,438,386 (GRCm39)	missense	probably benign	0.11
Z1176:Or10ag60	UTSW	2	87,438,098 (GRCm39)	missense	probably benign	0.34

Predicted Primers

PCR Primer
(F):5'- AGGGGCTACTATCAAACTCCAC -3'
(R):5'- CTTCTGTAGTGCAGGATCAATTCTAG -3'

Sequencing Primer
(F):5'- CTCCACAAAATACACAACATTTAGTG -3'
(R):5'- GTGCAGGATCAATTCTAGTTATCAC -3'

Posted On

2015-07-21