Mutagenetix > Incidental Mutation

Incidental Mutation 'R0069:Lzts3'

33083

Institutional Source

Beutler Lab

Gene Symbol

Lzts3

Ensembl Gene

ENSMUSG00000037703

Gene Name

leucine zipper, putative tumor suppressor family member 3

Synonyms

Prosapip1

MMRRC Submission

038360-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.206) question?

Stock #

R0069 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

130474759-130484723 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 130478460 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 213 (T213S)

Ref Sequence

ENSEMBL: ENSMUSP00000086990 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045761] [ENSMUST00000089561] [ENSMUST00000110260]

AlphaFold

A2AHG0

Predicted Effect

probably benign
Transcript: ENSMUST00000045761
AA Change: T213S

PolyPhen 2 Score 0.163 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000037109
Gene: ENSMUSG00000037703
AA Change: T213S

Domain	Start	End	E-Value	Type
low complexity region	86	107	N/A	INTRINSIC
low complexity region	147	158	N/A	INTRINSIC
low complexity region	240	250	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	285	297	N/A	INTRINSIC
low complexity region	301	333	N/A	INTRINSIC
low complexity region	337	346	N/A	INTRINSIC
low complexity region	349	361	N/A	INTRINSIC
low complexity region	402	436	N/A	INTRINSIC
Pfam:Fez1	465	665	1.4e-62	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000089561
AA Change: T213S

PolyPhen 2 Score 0.163 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000086990
Gene: ENSMUSG00000037703
AA Change: T213S

Domain	Start	End	E-Value	Type
low complexity region	86	107	N/A	INTRINSIC
low complexity region	147	158	N/A	INTRINSIC
low complexity region	240	250	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	285	297	N/A	INTRINSIC
low complexity region	301	333	N/A	INTRINSIC
low complexity region	337	346	N/A	INTRINSIC
low complexity region	349	361	N/A	INTRINSIC
low complexity region	402	436	N/A	INTRINSIC
Pfam:Fez1	465	666	2.1e-74	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000110260
AA Change: T99S

PolyPhen 2 Score 0.044 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000105889
Gene: ENSMUSG00000037703
AA Change: T99S

Domain	Start	End	E-Value	Type
low complexity region	126	136	N/A	INTRINSIC
low complexity region	139	151	N/A	INTRINSIC
low complexity region	171	183	N/A	INTRINSIC
low complexity region	187	219	N/A	INTRINSIC
low complexity region	223	232	N/A	INTRINSIC
low complexity region	235	247	N/A	INTRINSIC
low complexity region	288	322	N/A	INTRINSIC
Pfam:Fez1	351	552	1.5e-74	PFAM

Meta Mutation Damage Score

0.0582

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.2%
20x: 95.4%

Validation Efficiency

96% (52/54)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	A	T	6: 128,538,525 (GRCm39)	C632S	probably damaging	Het
Antxr2	T	A	5: 98,096,109 (GRCm39)	M392L	possibly damaging	Het
Cd101	A	G	3: 100,915,533 (GRCm39)	V678A	probably benign	Het
Clec2g	T	C	6: 128,957,274 (GRCm39)		probably null	Het
Clec2g	T	A	6: 128,925,716 (GRCm39)	S42T	probably benign	Het
Creb1	A	G	1: 64,615,367 (GRCm39)	I240V	possibly damaging	Het
D2hgdh	G	T	1: 93,763,009 (GRCm39)	V265L	possibly damaging	Het
Dctn2	A	T	10: 127,113,354 (GRCm39)		probably null	Het
Diablo	A	T	5: 123,656,087 (GRCm39)	S117R	probably damaging	Het
Ebf2	A	T	14: 67,647,499 (GRCm39)	R349S	probably damaging	Het
Fam168a	C	T	7: 100,484,618 (GRCm39)	A252V	probably benign	Het
Fbn2	T	C	18: 58,202,256 (GRCm39)	Y1299C	probably damaging	Het
Gne	A	C	4: 44,060,099 (GRCm39)	V98G	probably damaging	Het
Hk2	A	G	6: 82,713,509 (GRCm39)		probably null	Het
Ifi206	A	T	1: 173,314,413 (GRCm39)	V9D	probably damaging	Het
Ints3	A	G	3: 90,307,954 (GRCm39)		probably benign	Het
Itgal	A	G	7: 126,909,503 (GRCm39)	T56A	probably benign	Het
Map1b	A	G	13: 99,566,356 (GRCm39)	S2122P	unknown	Het
Mei4	C	T	9: 81,907,635 (GRCm39)	Q223*	probably null	Het
Mpzl3	T	C	9: 44,979,550 (GRCm39)	V167A	probably damaging	Het
Myo1d	A	G	11: 80,528,779 (GRCm39)	I681T	probably damaging	Het
Myom2	A	G	8: 15,167,624 (GRCm39)	T1070A	probably benign	Het
Nacc1	T	A	8: 85,403,828 (GRCm39)	I16F	probably damaging	Het
Nfx1	T	C	4: 40,986,688 (GRCm39)		probably benign	Het
Or10ak12	A	T	4: 118,666,887 (GRCm39)	V58D	probably damaging	Het
Or8g33	A	G	9: 39,338,188 (GRCm39)	Y60H	probably damaging	Het
Ostm1	A	C	10: 42,568,952 (GRCm39)	D37A	probably benign	Het
Pde8a	T	C	7: 80,968,871 (GRCm39)		probably benign	Het
Pole2	A	T	12: 69,256,661 (GRCm39)	V288E	probably damaging	Het
Poteg	T	C	8: 27,937,849 (GRCm39)	S2P	probably benign	Het
Ppp2r5c	A	T	12: 110,534,204 (GRCm39)	M356L	probably benign	Het
Prkdc	G	A	16: 15,544,368 (GRCm39)	S1786N	probably benign	Het
Prox1	A	G	1: 189,893,116 (GRCm39)	V443A	possibly damaging	Het
Prpf6	T	A	2: 181,257,756 (GRCm39)		probably null	Het
Ptger1	A	T	8: 84,394,948 (GRCm39)	T142S	possibly damaging	Het
Rad54l2	C	A	9: 106,587,564 (GRCm39)	V734L	possibly damaging	Het
Rnpepl1	T	A	1: 92,846,620 (GRCm39)	N507K	possibly damaging	Het
Slc38a10	A	T	11: 119,997,328 (GRCm39)	V722E	probably damaging	Het
Slfn10-ps	A	G	11: 82,926,368 (GRCm39)		noncoding transcript	Het
Slitrk6	A	T	14: 110,987,364 (GRCm39)	L781H	probably damaging	Het
Sult1e1	A	T	5: 87,727,756 (GRCm39)	H175Q	probably damaging	Het
Ube2e3	C	A	2: 78,750,293 (GRCm39)		probably benign	Het
Vmn1r208	A	T	13: 22,956,595 (GRCm39)	W301R	probably benign	Het
Vps13d	A	G	4: 144,789,133 (GRCm39)	I746T	probably benign	Het
Xpnpep3	T	C	15: 81,314,999 (GRCm39)	V233A	probably benign	Het
Zfp329	A	T	7: 12,544,859 (GRCm39)	S222T	probably damaging	Het
Zswim6	T	C	13: 107,875,098 (GRCm39)		noncoding transcript	Het

Other mutations in Lzts3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01072:Lzts3	APN	2	130,477,365 (GRCm39)	missense	probably damaging	0.99
IGL01541:Lzts3	APN	2	130,478,126 (GRCm39)	missense	probably damaging	0.99
IGL01649:Lzts3	APN	2	130,477,351 (GRCm39)	missense	probably damaging	0.98
IGL02746:Lzts3	APN	2	130,478,261 (GRCm39)	missense	probably damaging	0.98
IGL02927:Lzts3	APN	2	130,479,877 (GRCm39)	utr 5 prime	probably benign
R0069:Lzts3	UTSW	2	130,478,460 (GRCm39)	missense	probably benign	0.16
R0173:Lzts3	UTSW	2	130,476,688 (GRCm39)	makesense	probably null
R1381:Lzts3	UTSW	2	130,477,219 (GRCm39)	missense	probably damaging	0.99
R4127:Lzts3	UTSW	2	130,477,285 (GRCm39)	missense	probably damaging	0.99
R4301:Lzts3	UTSW	2	130,478,358 (GRCm39)	missense	probably damaging	0.99
R4588:Lzts3	UTSW	2	130,476,686 (GRCm39)	makesense	probably null
R5289:Lzts3	UTSW	2	130,478,021 (GRCm39)	missense	probably benign	0.18
R5878:Lzts3	UTSW	2	130,478,459 (GRCm39)	missense	probably damaging	1.00
R5964:Lzts3	UTSW	2	130,478,208 (GRCm39)	missense	probably damaging	0.99
R6193:Lzts3	UTSW	2	130,479,306 (GRCm39)	missense	probably damaging	0.97
R7692:Lzts3	UTSW	2	130,477,306 (GRCm39)	missense	probably benign	0.27
R8195:Lzts3	UTSW	2	130,477,105 (GRCm39)	missense	probably benign	0.01
R8273:Lzts3	UTSW	2	130,476,801 (GRCm39)	missense	possibly damaging	0.66
R9126:Lzts3	UTSW	2	130,477,248 (GRCm39)	missense	possibly damaging	0.66
R9129:Lzts3	UTSW	2	130,476,865 (GRCm39)	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- ACTATAGCCAGCGGTGCCAATACG -3'
(R):5'- GCAGGAGGTGGTTCTTGAACAAGTC -3'

Sequencing Primer
(F):5'- CGGTGCCAATACGGTTGATATG -3'
(R):5'- CTTGAACAAGTCTTGGGCG -3'

Posted On

2013-05-09