Incidental Mutation 'R4495:Mrgpra3'
| ID |
330932 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mrgpra3
|
| Ensembl Gene |
ENSMUSG00000078698 |
| Gene Name |
MAS-related GPR, member A3 |
| Synonyms |
G protein-coupled receptor, MrgA3 |
| MMRRC Submission |
041583-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.048)
|
| Stock # |
R4495 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
47238698-47251120 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 47239813 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Valine
at position 38
(I38V)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000135437
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000176369]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176369
AA Change: I38V
PolyPhen 2
Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
|
| SMART Domains |
Protein: ENSMUSP00000135437
Gene: ENSMUSG00000078698
AA Change: I38V
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
14 |
31 |
N/A |
INTRINSIC |
|
Pfam:7TM_GPCR_Srx
|
48 |
234 |
6.3e-7 |
PFAM |
|
Pfam:7tm_1
|
57 |
286 |
2.7e-9 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 95.1%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 37 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Calcr |
A |
T |
6: 3,708,484 (GRCm39) |
|
probably null |
Het |
| Cdh4 |
T |
C |
2: 179,422,182 (GRCm39) |
V102A |
probably damaging |
Het |
| Cmya5 |
T |
C |
13: 93,230,573 (GRCm39) |
E1505G |
probably benign |
Het |
| Cyp2b9 |
T |
A |
7: 25,900,180 (GRCm39) |
D329E |
probably benign |
Het |
| Ddx47 |
T |
C |
6: 134,998,429 (GRCm39) |
F375S |
possibly damaging |
Het |
| Dnah7b |
T |
G |
1: 46,124,792 (GRCm39) |
S154A |
probably benign |
Het |
| Fry |
G |
T |
5: 150,233,928 (GRCm39) |
E133D |
probably damaging |
Het |
| Hydin |
T |
C |
8: 111,322,034 (GRCm39) |
L4562P |
probably damaging |
Het |
| Ifnlr1 |
A |
T |
4: 135,433,079 (GRCm39) |
E505V |
probably damaging |
Het |
| Igfn1 |
T |
C |
1: 135,897,416 (GRCm39) |
E1050G |
possibly damaging |
Het |
| Kidins220 |
T |
A |
12: 25,088,301 (GRCm39) |
|
probably null |
Het |
| Klra10 |
T |
G |
6: 130,256,311 (GRCm39) |
E114D |
probably benign |
Het |
| Lyst |
G |
A |
13: 13,809,968 (GRCm39) |
R546H |
probably damaging |
Het |
| Mrc2 |
G |
A |
11: 105,239,257 (GRCm39) |
|
probably null |
Het |
| Nfkb2 |
A |
G |
19: 46,296,878 (GRCm39) |
D316G |
probably damaging |
Het |
| Nrxn2 |
A |
T |
19: 6,581,429 (GRCm39) |
T412S |
probably benign |
Het |
| Or4k37 |
T |
A |
2: 111,159,365 (GRCm39) |
N200K |
probably benign |
Het |
| Palm3 |
C |
T |
8: 84,753,495 (GRCm39) |
R97C |
probably damaging |
Het |
| Pdcd11 |
T |
C |
19: 47,099,445 (GRCm39) |
V848A |
probably benign |
Het |
| Prdx1 |
T |
C |
4: 116,556,416 (GRCm39) |
V188A |
probably benign |
Het |
| Prep |
T |
C |
10: 44,996,915 (GRCm39) |
F398L |
probably benign |
Het |
| Prlhr |
A |
T |
19: 60,455,519 (GRCm39) |
M349K |
probably benign |
Het |
| Rwdd2b |
T |
C |
16: 87,231,450 (GRCm39) |
T235A |
probably benign |
Het |
| Scn1a |
T |
C |
2: 66,111,146 (GRCm39) |
|
probably null |
Het |
| Sidt1 |
C |
T |
16: 44,102,841 (GRCm39) |
V295M |
probably damaging |
Het |
| Sla |
T |
C |
15: 66,673,361 (GRCm39) |
T10A |
probably benign |
Het |
| Slc22a20 |
A |
G |
19: 6,034,952 (GRCm39) |
S170P |
probably benign |
Het |
| Syt6 |
C |
A |
3: 103,494,876 (GRCm39) |
C280* |
probably null |
Het |
| Thbs2 |
A |
G |
17: 14,891,675 (GRCm39) |
I954T |
probably damaging |
Het |
| Tmprss11b |
T |
A |
5: 86,812,922 (GRCm39) |
K125* |
probably null |
Het |
| Ugt1a6a |
T |
A |
1: 88,066,905 (GRCm39) |
L237H |
probably damaging |
Het |
| Vmn2r68 |
TCC |
TC |
7: 84,870,758 (GRCm39) |
|
probably null |
Het |
| Wdr53 |
A |
G |
16: 32,070,969 (GRCm39) |
T105A |
probably benign |
Het |
| Xkrx |
T |
C |
X: 133,051,745 (GRCm39) |
N302S |
possibly damaging |
Het |
| Zfp704 |
T |
A |
3: 9,536,137 (GRCm39) |
S128C |
probably benign |
Het |
| Zfp759 |
A |
G |
13: 67,286,989 (GRCm39) |
|
probably null |
Het |
| Zfyve16 |
A |
T |
13: 92,625,075 (GRCm39) |
D1494E |
probably benign |
Het |
|
| Other mutations in Mrgpra3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00162:Mrgpra3
|
APN |
7 |
47,239,267 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02097:Mrgpra3
|
APN |
7 |
47,239,204 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
IGL02101:Mrgpra3
|
APN |
7 |
47,239,300 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL03071:Mrgpra3
|
APN |
7 |
47,238,929 (GRCm39) |
utr 3 prime |
probably benign |
|
|
PIT4445001:Mrgpra3
|
UTSW |
7 |
47,239,908 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R0828:Mrgpra3
|
UTSW |
7 |
47,239,884 (GRCm39) |
missense |
probably benign |
0.12 |
|
R1118:Mrgpra3
|
UTSW |
7 |
47,239,039 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R1835:Mrgpra3
|
UTSW |
7 |
47,239,694 (GRCm39) |
nonsense |
probably null |
|
|
R2258:Mrgpra3
|
UTSW |
7 |
47,239,842 (GRCm39) |
missense |
probably benign |
0.35 |
|
R2393:Mrgpra3
|
UTSW |
7 |
47,239,365 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R3437:Mrgpra3
|
UTSW |
7 |
47,239,314 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3973:Mrgpra3
|
UTSW |
7 |
47,239,414 (GRCm39) |
missense |
probably benign |
0.22 |
|
R4273:Mrgpra3
|
UTSW |
7 |
47,239,180 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4768:Mrgpra3
|
UTSW |
7 |
47,239,476 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R4822:Mrgpra3
|
UTSW |
7 |
47,239,716 (GRCm39) |
missense |
possibly damaging |
0.69 |
|
R4967:Mrgpra3
|
UTSW |
7 |
47,239,267 (GRCm39) |
missense |
probably benign |
0.33 |
|
R5207:Mrgpra3
|
UTSW |
7 |
47,239,909 (GRCm39) |
missense |
probably benign |
0.06 |
|
R5569:Mrgpra3
|
UTSW |
7 |
47,239,759 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5763:Mrgpra3
|
UTSW |
7 |
47,239,355 (GRCm39) |
nonsense |
probably null |
|
|
R6102:Mrgpra3
|
UTSW |
7 |
47,239,897 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R6612:Mrgpra3
|
UTSW |
7 |
47,239,783 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6718:Mrgpra3
|
UTSW |
7 |
47,239,444 (GRCm39) |
missense |
probably benign |
0.25 |
|
R6859:Mrgpra3
|
UTSW |
7 |
47,239,781 (GRCm39) |
missense |
probably benign |
0.04 |
|
R7029:Mrgpra3
|
UTSW |
7 |
47,239,290 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7034:Mrgpra3
|
UTSW |
7 |
47,239,838 (GRCm39) |
missense |
possibly damaging |
0.49 |
|
R7036:Mrgpra3
|
UTSW |
7 |
47,239,838 (GRCm39) |
missense |
possibly damaging |
0.49 |
|
R7097:Mrgpra3
|
UTSW |
7 |
47,239,389 (GRCm39) |
missense |
probably benign |
|
|
R7475:Mrgpra3
|
UTSW |
7 |
47,239,695 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8289:Mrgpra3
|
UTSW |
7 |
47,239,468 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
R8315:Mrgpra3
|
UTSW |
7 |
47,251,051 (GRCm39) |
start codon destroyed |
probably null |
0.93 |
|
R9500:Mrgpra3
|
UTSW |
7 |
47,239,400 (GRCm39) |
nonsense |
probably null |
|
|
Z1177:Mrgpra3
|
UTSW |
7 |
47,251,049 (GRCm39) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- CCACTGTGGAATTTATGATGTGACAG -3'
(R):5'- GTTGACGAACACCCTCATAGAAGC -3'
Sequencing Primer
(F):5'- TGGAATTTATGATGTGACAGAGAAG -3'
(R):5'- CACCCTCATAGAAGCAGGGG -3'
|
| Posted On |
2015-07-21 |
Incidental Mutation