Incidental Mutation 'R4482:Rasl10a'
| ID |
331468 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Rasl10a
|
| Ensembl Gene |
ENSMUSG00000034209 |
| Gene Name |
RAS-like, family 10, member A |
| Synonyms |
2210403B10Rik |
| MMRRC Submission |
041738-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.116)
|
| Stock # |
R4482 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
11 |
| Chromosomal Location |
5008128-5010385 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 5008429 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Asparagine
at position 42
(Y42N)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000048453
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000037146]
[ENSMUST00000037218]
[ENSMUST00000056649]
[ENSMUST00000109895]
|
| AlphaFold |
Q8K5A4 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000037146
|
| SMART Domains |
Protein: ENSMUSP00000043709
Gene: ENSMUSG00000034201
| Domain | Start | End | E-Value | Type |
|---|
|
CH
|
29 |
143 |
2.69e-16 |
SMART |
|
GAS2
|
206 |
278 |
7.69e-52 |
SMART |
|
low complexity region
|
292 |
318 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000037218
AA Change: Y42N
PolyPhen 2
Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000048453
Gene: ENSMUSG00000034209
AA Change: Y42N
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Ras
|
6 |
180 |
1.5e-20 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000056649
|
| SMART Domains |
Protein: ENSMUSP00000050275
Gene: ENSMUSG00000034201
| Domain | Start | End | E-Value | Type |
|---|
|
CH
|
29 |
143 |
2.69e-16 |
SMART |
|
GAS2
|
206 |
278 |
7.69e-52 |
SMART |
|
low complexity region
|
292 |
318 |
N/A |
INTRINSIC |
|
low complexity region
|
335 |
365 |
N/A |
INTRINSIC |
|
low complexity region
|
392 |
406 |
N/A |
INTRINSIC |
|
low complexity region
|
411 |
423 |
N/A |
INTRINSIC |
|
low complexity region
|
460 |
473 |
N/A |
INTRINSIC |
|
low complexity region
|
478 |
496 |
N/A |
INTRINSIC |
|
low complexity region
|
509 |
539 |
N/A |
INTRINSIC |
|
low complexity region
|
546 |
568 |
N/A |
INTRINSIC |
|
low complexity region
|
641 |
657 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000109895
|
| SMART Domains |
Protein: ENSMUSP00000105521
Gene: ENSMUSG00000034201
| Domain | Start | End | E-Value | Type |
|---|
|
CH
|
29 |
143 |
2.69e-16 |
SMART |
|
GAS2
|
206 |
278 |
7.69e-52 |
SMART |
|
low complexity region
|
292 |
318 |
N/A |
INTRINSIC |
|
low complexity region
|
335 |
365 |
N/A |
INTRINSIC |
|
low complexity region
|
392 |
406 |
N/A |
INTRINSIC |
|
low complexity region
|
411 |
423 |
N/A |
INTRINSIC |
|
low complexity region
|
460 |
473 |
N/A |
INTRINSIC |
|
low complexity region
|
478 |
496 |
N/A |
INTRINSIC |
|
low complexity region
|
509 |
539 |
N/A |
INTRINSIC |
|
low complexity region
|
546 |
568 |
N/A |
INTRINSIC |
|
low complexity region
|
641 |
657 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000122886
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000126974
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000156196
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 97.1%
- 20x: 94.9%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 31 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4921536K21Rik |
A |
G |
11: 3,845,008 (GRCm39) |
V27A |
possibly damaging |
Het |
| Actn3 |
A |
T |
19: 4,913,436 (GRCm39) |
|
probably null |
Het |
| Adamts20 |
T |
C |
15: 94,243,801 (GRCm39) |
Y642C |
probably damaging |
Het |
| Ankrd11 |
T |
C |
8: 123,620,228 (GRCm39) |
D1187G |
probably damaging |
Het |
| Bag4 |
T |
C |
8: 26,275,072 (GRCm39) |
|
probably benign |
Het |
| Bsn |
C |
A |
9: 107,991,863 (GRCm39) |
K1296N |
probably damaging |
Het |
| Cct5 |
T |
C |
15: 31,597,715 (GRCm39) |
D58G |
probably damaging |
Het |
| Cyp4a10 |
T |
C |
4: 115,389,795 (GRCm39) |
F467L |
probably damaging |
Het |
| Dicer1 |
A |
G |
12: 104,672,536 (GRCm39) |
Y904H |
probably damaging |
Het |
| Fbn1 |
A |
T |
2: 125,205,530 (GRCm39) |
|
probably null |
Het |
| Fhip1b |
A |
T |
7: 105,038,881 (GRCm39) |
F119L |
probably benign |
Het |
| Git1 |
T |
A |
11: 77,391,333 (GRCm39) |
Y189N |
possibly damaging |
Het |
| Homer3 |
G |
A |
8: 70,742,793 (GRCm39) |
|
probably null |
Het |
| Itga6 |
T |
A |
2: 71,686,259 (GRCm39) |
I1059N |
probably damaging |
Het |
| Kalrn |
C |
T |
16: 33,810,180 (GRCm39) |
D2525N |
possibly damaging |
Het |
| Kbtbd3 |
A |
T |
9: 4,331,051 (GRCm39) |
Y475F |
probably damaging |
Het |
| Map3k6 |
T |
C |
4: 132,970,710 (GRCm39) |
I199T |
probably benign |
Het |
| Mtmr7 |
T |
C |
8: 41,007,425 (GRCm39) |
M503V |
probably benign |
Het |
| Muc4 |
A |
G |
16: 32,577,075 (GRCm39) |
T2192A |
unknown |
Het |
| Nexn |
T |
C |
3: 151,948,390 (GRCm39) |
E391G |
probably damaging |
Het |
| Or5h22 |
G |
T |
16: 58,895,286 (GRCm39) |
H52Q |
probably benign |
Het |
| Parg |
A |
G |
14: 31,984,731 (GRCm39) |
I254V |
probably damaging |
Het |
| Plxna2 |
C |
T |
1: 194,431,625 (GRCm39) |
S538F |
probably damaging |
Het |
| Pomt2 |
G |
A |
12: 87,178,604 (GRCm39) |
P290S |
probably benign |
Het |
| Slc4a8 |
C |
A |
15: 100,708,480 (GRCm39) |
F871L |
probably damaging |
Het |
| Tdrd9 |
T |
C |
12: 111,980,935 (GRCm39) |
|
probably null |
Het |
| Trim21 |
G |
A |
7: 102,213,140 (GRCm39) |
Q53* |
probably null |
Het |
| Triobp |
T |
C |
15: 78,850,763 (GRCm39) |
S306P |
possibly damaging |
Het |
| Vasn |
A |
T |
16: 4,466,190 (GRCm39) |
T46S |
possibly damaging |
Het |
| Wmp |
T |
A |
X: 106,990,237 (GRCm39) |
L225F |
possibly damaging |
Het |
| Zfp236 |
A |
T |
18: 82,662,346 (GRCm39) |
F529Y |
probably benign |
Het |
|
| Other mutations in Rasl10a |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02233:Rasl10a
|
APN |
11 |
5,008,333 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL03149:Rasl10a
|
APN |
11 |
5,008,429 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
R1630:Rasl10a
|
UTSW |
11 |
5,009,542 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1678:Rasl10a
|
UTSW |
11 |
5,009,815 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1925:Rasl10a
|
UTSW |
11 |
5,009,473 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R2086:Rasl10a
|
UTSW |
11 |
5,009,431 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
R3792:Rasl10a
|
UTSW |
11 |
5,009,461 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4719:Rasl10a
|
UTSW |
11 |
5,008,517 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5743:Rasl10a
|
UTSW |
11 |
5,009,519 (GRCm39) |
missense |
probably benign |
0.02 |
|
R6168:Rasl10a
|
UTSW |
11 |
5,008,442 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
R6530:Rasl10a
|
UTSW |
11 |
5,008,367 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6684:Rasl10a
|
UTSW |
11 |
5,008,396 (GRCm39) |
missense |
possibly damaging |
0.49 |
|
R8049:Rasl10a
|
UTSW |
11 |
5,009,823 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9144:Rasl10a
|
UTSW |
11 |
5,008,473 (GRCm39) |
missense |
probably benign |
0.31 |
|
| Predicted Primers |
PCR Primer
(F):5'- GCGGCTTCGGTTTTGAAAC -3'
(R):5'- GCAGGAAGCTCGTATGCAAAC -3'
Sequencing Primer
(F):5'- GACAGTTACCTCATCGCCG -3'
(R):5'- GGAAGCTCGTATGCAAACTACCTAG -3'
|
| Posted On |
2015-07-21 |
Incidental Mutation