Mutagenetix > Incidental Mutation

Incidental Mutation 'R4485:Vmn2r87'

331582

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r87

Ensembl Gene

ENSMUSG00000091511

Gene Name

vomeronasal 2, receptor 87

Synonyms

EG625131

MMRRC Submission

041741-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R4485 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

130307690-130333248 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 130315678 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 129 (Y129*)

Ref Sequence

ENSEMBL: ENSMUSP00000129215 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164227]

AlphaFold

E9PZX4

Predicted Effect

probably null
Transcript: ENSMUST00000164227
AA Change: Y129*

SMART Domains

Protein: ENSMUSP00000129215
Gene: ENSMUSG00000091511
AA Change: Y129*

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	422	1.8e-27	PFAM
Pfam:NCD3G	508	562	1.8e-19	PFAM
Pfam:7tm_3	595	829	8.8e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agxt2	A	T	15: 10,378,968 (GRCm39)	I190L	possibly damaging	Het
Ahnak2	A	G	12: 112,745,944 (GRCm39)		probably benign	Het
Apol7b	C	A	15: 77,307,866 (GRCm39)	V210L	probably benign	Het
Banf2	C	T	2: 143,915,772 (GRCm39)	T71M	probably damaging	Het
Cacna1s	A	G	1: 136,004,590 (GRCm39)	D130G	probably damaging	Het
Capn8	T	C	1: 182,426,306 (GRCm39)	F214L	possibly damaging	Het
Ccdc168	A	G	1: 44,099,283 (GRCm39)	L605S	probably benign	Het
Cdk5rap2	A	T	4: 70,157,520 (GRCm39)		probably null	Het
Cldn8	A	C	16: 88,359,619 (GRCm39)	M102R	probably damaging	Het
Cpb1	T	G	3: 20,303,865 (GRCm39)	M400L	probably benign	Het
Eps15l1	A	T	8: 73,153,531 (GRCm39)	I52N	possibly damaging	Het
Gbp9	C	G	5: 105,231,674 (GRCm39)	G304A	probably damaging	Het
Gm14226	G	A	2: 154,867,191 (GRCm39)	V383I	probably benign	Het
Itprid1	A	G	6: 55,864,051 (GRCm39)	T126A	probably benign	Het
Npm2	A	T	14: 70,885,749 (GRCm39)	V152D	possibly damaging	Het
Nsd1	T	C	13: 55,393,434 (GRCm39)	V345A	probably benign	Het
Or56a3	T	A	7: 104,735,808 (GRCm39)	V295D	probably damaging	Het
Or5b107	T	C	19: 13,142,855 (GRCm39)	V159A	possibly damaging	Het
Or5b118	T	C	19: 13,448,919 (GRCm39)	I195T	probably benign	Het
Or5p58	T	C	7: 107,694,222 (GRCm39)	D185G	probably benign	Het
Otof	T	A	5: 30,532,344 (GRCm39)	H1601L	possibly damaging	Het
Plat	G	A	8: 23,262,228 (GRCm39)	S84N	probably benign	Het
Prss30	T	C	17: 24,192,130 (GRCm39)	D224G	probably damaging	Het
Psg25	C	A	7: 18,260,203 (GRCm39)	V232F	probably damaging	Het
Ptch1	C	T	13: 63,682,143 (GRCm39)	R537H	probably damaging	Het
Pus7l	G	T	15: 94,421,371 (GRCm39)	H646Q	probably benign	Het
Ryr1	C	T	7: 28,789,581 (GRCm39)	S1511N	probably damaging	Het
Slc16a1	A	G	3: 104,562,794 (GRCm39)	K413R	probably benign	Het
Snrnp48	T	A	13: 38,400,304 (GRCm39)	M137K	probably benign	Het
Tecta	T	A	9: 42,248,570 (GRCm39)	H1944L	possibly damaging	Het
Tmem145	A	G	7: 25,006,587 (GRCm39)	E65G	possibly damaging	Het
Wdr7	A	G	18: 63,910,621 (GRCm39)	H671R	possibly damaging	Het
Zfp219	A	G	14: 52,244,841 (GRCm39)	V518A	probably damaging	Het
Zfp472	T	C	17: 33,196,542 (GRCm39)	W206R	possibly damaging	Het

Other mutations in Vmn2r87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01090:Vmn2r87	APN	10	130,333,247 (GRCm39)	start codon destroyed	probably null	1.00
IGL01295:Vmn2r87	APN	10	130,307,878 (GRCm39)	missense	probably damaging	1.00
IGL01411:Vmn2r87	APN	10	130,308,429 (GRCm39)	missense	probably benign	0.03
IGL01680:Vmn2r87	APN	10	130,315,586 (GRCm39)	nonsense	probably null
IGL01822:Vmn2r87	APN	10	130,307,991 (GRCm39)	missense	probably damaging	1.00
IGL01835:Vmn2r87	APN	10	130,314,978 (GRCm39)	missense	probably damaging	1.00
IGL01965:Vmn2r87	APN	10	130,314,924 (GRCm39)	missense	possibly damaging	0.49
IGL02562:Vmn2r87	APN	10	130,314,513 (GRCm39)	missense	probably damaging	1.00
IGL02665:Vmn2r87	APN	10	130,333,049 (GRCm39)	missense	probably benign	0.16
IGL03202:Vmn2r87	APN	10	130,333,091 (GRCm39)	missense	probably benign
FR4304:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4340:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4342:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4589:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
LCD18:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
R0344:Vmn2r87	UTSW	10	130,315,806 (GRCm39)	missense	probably damaging	1.00
R0374:Vmn2r87	UTSW	10	130,307,848 (GRCm39)	missense	probably damaging	1.00
R0384:Vmn2r87	UTSW	10	130,307,712 (GRCm39)	missense	probably benign
R1144:Vmn2r87	UTSW	10	130,312,098 (GRCm39)	splice site	probably benign
R1172:Vmn2r87	UTSW	10	130,313,453 (GRCm39)	missense	probably benign	0.03
R1860:Vmn2r87	UTSW	10	130,315,755 (GRCm39)	missense	probably benign	0.00
R1866:Vmn2r87	UTSW	10	130,308,441 (GRCm39)	missense	possibly damaging	0.88
R1897:Vmn2r87	UTSW	10	130,307,829 (GRCm39)	missense	probably damaging	1.00
R2360:Vmn2r87	UTSW	10	130,315,631 (GRCm39)	missense	probably damaging	0.99
R2909:Vmn2r87	UTSW	10	130,314,865 (GRCm39)	missense	probably damaging	0.99
R3874:Vmn2r87	UTSW	10	130,315,856 (GRCm39)	missense	possibly damaging	0.62
R4113:Vmn2r87	UTSW	10	130,315,691 (GRCm39)	missense	probably benign
R4190:Vmn2r87	UTSW	10	130,308,556 (GRCm39)	missense	probably damaging	1.00
R4197:Vmn2r87	UTSW	10	130,315,779 (GRCm39)	missense	possibly damaging	0.55
R4201:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4202:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4368:Vmn2r87	UTSW	10	130,315,676 (GRCm39)	missense	probably benign	0.44
R4537:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably benign	0.12
R4590:Vmn2r87	UTSW	10	130,315,014 (GRCm39)	missense	possibly damaging	0.69
R4752:Vmn2r87	UTSW	10	130,314,336 (GRCm39)	nonsense	probably null
R4873:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4875:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4923:Vmn2r87	UTSW	10	130,314,435 (GRCm39)	missense	probably damaging	0.99
R4970:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5049:Vmn2r87	UTSW	10	130,308,298 (GRCm39)	missense	probably damaging	0.96
R5112:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5187:Vmn2r87	UTSW	10	130,333,208 (GRCm39)	missense	probably null	0.99
R5618:Vmn2r87	UTSW	10	130,315,817 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r87	UTSW	10	130,308,226 (GRCm39)	missense	probably benign	0.02
R6220:Vmn2r87	UTSW	10	130,315,807 (GRCm39)	missense	probably benign	0.01
R6287:Vmn2r87	UTSW	10	130,314,291 (GRCm39)	critical splice donor site	probably null
R6383:Vmn2r87	UTSW	10	130,314,869 (GRCm39)	missense	probably damaging	1.00
R6576:Vmn2r87	UTSW	10	130,314,654 (GRCm39)	missense	probably benign	0.05
R6742:Vmn2r87	UTSW	10	130,308,396 (GRCm39)	missense	probably damaging	1.00
R7086:Vmn2r87	UTSW	10	130,333,178 (GRCm39)	missense	probably benign	0.00
R7162:Vmn2r87	UTSW	10	130,313,416 (GRCm39)	missense	probably benign	0.08
R7419:Vmn2r87	UTSW	10	130,307,992 (GRCm39)	missense	probably damaging	1.00
R7425:Vmn2r87	UTSW	10	130,314,761 (GRCm39)	missense	probably damaging	1.00
R7443:Vmn2r87	UTSW	10	130,308,588 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r87	UTSW	10	130,314,940 (GRCm39)	missense	probably damaging	0.99
R7663:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably damaging	0.97
R7716:Vmn2r87	UTSW	10	130,308,018 (GRCm39)	missense	probably benign	0.09
R7793:Vmn2r87	UTSW	10	130,313,413 (GRCm39)	missense	probably benign	0.05
R7806:Vmn2r87	UTSW	10	130,315,679 (GRCm39)	missense	probably benign
R7841:Vmn2r87	UTSW	10	130,333,095 (GRCm39)	missense	probably benign	0.31
R8326:Vmn2r87	UTSW	10	130,308,180 (GRCm39)	missense	possibly damaging	0.67
R8411:Vmn2r87	UTSW	10	130,308,126 (GRCm39)	missense	probably damaging	0.99
R8445:Vmn2r87	UTSW	10	130,313,335 (GRCm39)	missense	probably damaging	1.00
R8540:Vmn2r87	UTSW	10	130,314,762 (GRCm39)	missense	possibly damaging	0.74
R8892:Vmn2r87	UTSW	10	130,308,105 (GRCm39)	missense	probably damaging	0.99
R9093:Vmn2r87	UTSW	10	130,308,165 (GRCm39)	missense	probably benign	0.08
R9623:Vmn2r87	UTSW	10	130,315,794 (GRCm39)	missense	probably damaging	1.00
R9667:Vmn2r87	UTSW	10	130,314,776 (GRCm39)	missense	probably damaging	1.00
R9797:Vmn2r87	UTSW	10	130,312,064 (GRCm39)	missense	probably benign	0.44
R9797:Vmn2r87	UTSW	10	130,308,138 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r87	UTSW	10	130,308,183 (GRCm39)	missense	probably damaging	0.98
Z1176:Vmn2r87	UTSW	10	130,307,713 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- GCTGTCCAACTTTTCTTGTAGG -3'
(R):5'- TCATACTATGAACTTTCCCGCAATG -3'

Sequencing Primer
(F):5'- TCTTGTAGGCACCTGTAAATGG -3'
(R):5'- GACTTGTTTTAGAATACCAGCAAGTG -3'

Posted On

2015-07-21