Mutagenetix > Incidental Mutation

Incidental Mutation 'R4506:Gpr158'

332001

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

MMRRC Submission

041584-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4506 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

21372378-21835355 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 21831810 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 970 (M970K)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

probably damaging
Transcript: ENSMUST00000055946
AA Change: M970K

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: M970K

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Meta Mutation Damage Score

0.1418

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

98% (42/43)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	G	T	11: 109,685,087 (GRCm39)	P137T	probably damaging	Het
Abcc5	A	T	16: 20,152,445 (GRCm39)	I1367N	probably damaging	Het
Ace	T	A	11: 105,867,492 (GRCm39)	L152Q	probably damaging	Het
Adam19	G	A	11: 46,009,271 (GRCm39)	D232N	possibly damaging	Het
Anapc4	A	T	5: 52,993,072 (GRCm39)	N61I	possibly damaging	Het
Ap5m1	T	C	14: 49,311,218 (GRCm39)	V96A	probably damaging	Het
Atr	T	A	9: 95,747,290 (GRCm39)	C191S	probably benign	Het
Carmil3	GGACGA	GGA	14: 55,736,933 (GRCm39)		probably benign	Het
Cnot6l	A	T	5: 96,234,033 (GRCm39)	V326E	possibly damaging	Het
Fcer2a	A	T	8: 3,738,603 (GRCm39)		probably null	Het
Fgfr3	A	G	5: 33,887,343 (GRCm39)	T221A	probably damaging	Het
Gm6632	C	T	5: 59,211,821 (GRCm39)		noncoding transcript	Het
H2-T5	A	T	17: 36,472,372 (GRCm39)		probably benign	Het
Ip6k3	A	T	17: 27,364,154 (GRCm39)	L298Q	possibly damaging	Het
Itpr1	A	G	6: 108,409,647 (GRCm39)	D1727G	probably damaging	Het
Kdm2b	T	C	5: 123,026,688 (GRCm39)	T589A	possibly damaging	Het
Krtcap2	A	G	3: 89,153,563 (GRCm39)		probably benign	Het
Map3k13	A	G	16: 21,740,928 (GRCm39)	S752G	probably benign	Het
Mvb12a	G	T	8: 71,996,103 (GRCm39)	A86S	probably benign	Het
Nktr	T	A	9: 121,577,949 (GRCm39)		probably benign	Het
Or1e33	A	T	11: 73,738,521 (GRCm39)	C143*	probably null	Het
Or8a1b	C	T	9: 37,622,760 (GRCm39)	V272I	probably benign	Het
Parp4	T	A	14: 56,889,761 (GRCm39)	N1847K	unknown	Het
Pcdhga8	G	C	18: 37,949,816 (GRCm39)	V411L	probably damaging	Het
Piwil4	T	C	9: 14,637,259 (GRCm39)	T352A	probably damaging	Het
Ric8a	A	G	7: 140,438,429 (GRCm39)	I223V	probably benign	Het
Slc25a51	C	T	4: 45,399,768 (GRCm39)	V141M	probably benign	Het
Slc7a5	A	T	8: 122,614,234 (GRCm39)		probably null	Het
Slc7a9	A	T	7: 35,152,845 (GRCm39)	T88S	probably damaging	Het
Tbrg1	T	A	9: 37,565,691 (GRCm39)	E87V	probably damaging	Het
Tnfsf4	T	C	1: 161,244,745 (GRCm39)	S145P	probably damaging	Het
Tomm34	C	A	2: 163,896,292 (GRCm39)		probably null	Het
Trpv3	A	G	11: 73,186,150 (GRCm39)	N647S	probably benign	Het
Upf1	G	A	8: 70,790,216 (GRCm39)	R637C	probably damaging	Het
Vmn2r23	A	T	6: 123,679,884 (GRCm39)	Q35H	probably damaging	Het
Wdtc1	C	T	4: 133,036,130 (GRCm39)	V137M	probably damaging	Het
Zfp276	G	A	8: 123,991,666 (GRCm39)		probably null	Het
Zfp90	C	T	8: 107,151,496 (GRCm39)	P403L	possibly damaging	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21,373,494 (GRCm39)	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21,751,606 (GRCm39)	splice site	probably benign
IGL00706:Gpr158	APN	2	21,751,584 (GRCm39)	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21,831,629 (GRCm39)	nonsense	probably null
IGL00885:Gpr158	APN	2	21,653,832 (GRCm39)	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21,373,842 (GRCm39)	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21,831,909 (GRCm39)	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21,788,101 (GRCm39)	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21,373,511 (GRCm39)	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21,831,407 (GRCm39)	missense	probably benign
IGL02681:Gpr158	APN	2	21,820,441 (GRCm39)	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21,831,638 (GRCm39)	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21,831,890 (GRCm39)	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21,787,972 (GRCm39)	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21,831,057 (GRCm39)	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21,831,682 (GRCm39)	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21,815,479 (GRCm39)	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21,831,528 (GRCm39)	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21,830,019 (GRCm39)	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21,820,480 (GRCm39)	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21,831,125 (GRCm39)	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21,820,505 (GRCm39)	splice site	probably null
R1609:Gpr158	UTSW	2	21,788,104 (GRCm39)	missense	possibly damaging	0.61
R1741:Gpr158	UTSW	2	21,832,359 (GRCm39)	missense	probably benign	0.00
R1827:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R1854:Gpr158	UTSW	2	21,373,935 (GRCm39)	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21,820,426 (GRCm39)	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21,832,325 (GRCm39)	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R2275:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R3004:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21,581,771 (GRCm39)	missense	possibly damaging	0.68
R3943:Gpr158	UTSW	2	21,373,370 (GRCm39)	missense	possibly damaging	0.65
R4238:Gpr158	UTSW	2	21,373,362 (GRCm39)	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21,830,025 (GRCm39)	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21,832,403 (GRCm39)	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21,373,811 (GRCm39)	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21,831,864 (GRCm39)	missense	probably benign
R4798:Gpr158	UTSW	2	21,787,993 (GRCm39)	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21,830,059 (GRCm39)	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21,831,968 (GRCm39)	missense	probably damaging	1.00
R5334:Gpr158	UTSW	2	21,832,316 (GRCm39)	missense	probably benign	0.01
R5560:Gpr158	UTSW	2	21,831,101 (GRCm39)	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21,832,046 (GRCm39)	missense	probably benign
R5637:Gpr158	UTSW	2	21,788,083 (GRCm39)	missense	probably benign	0.00
R5701:Gpr158	UTSW	2	21,751,520 (GRCm39)	missense	probably damaging	1.00
R5744:Gpr158	UTSW	2	21,373,331 (GRCm39)	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21,373,932 (GRCm39)	missense	possibly damaging	0.95
R5991:Gpr158	UTSW	2	21,373,319 (GRCm39)	missense	probably damaging	0.99
R6200:Gpr158	UTSW	2	21,404,227 (GRCm39)	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21,820,422 (GRCm39)	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21,815,365 (GRCm39)	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21,831,099 (GRCm39)	missense	probably damaging	1.00
R6698:Gpr158	UTSW	2	21,831,921 (GRCm39)	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21,653,802 (GRCm39)	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21,831,386 (GRCm39)	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21,373,113 (GRCm39)	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21,815,412 (GRCm39)	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21,581,750 (GRCm39)	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R7515:Gpr158	UTSW	2	21,373,092 (GRCm39)	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21,831,158 (GRCm39)	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R8311:Gpr158	UTSW	2	21,373,701 (GRCm39)	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21,581,693 (GRCm39)	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21,404,149 (GRCm39)	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21,558,137 (GRCm39)	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21,581,751 (GRCm39)	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21,581,760 (GRCm39)	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21,830,078 (GRCm39)	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21,831,251 (GRCm39)	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9317:Gpr158	UTSW	2	21,832,037 (GRCm39)	missense	probably benign
R9379:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9428:Gpr158	UTSW	2	21,787,972 (GRCm39)	missense	probably benign
R9497:Gpr158	UTSW	2	21,831,825 (GRCm39)	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21,830,054 (GRCm39)	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21,831,315 (GRCm39)	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21,831,180 (GRCm39)	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21,815,501 (GRCm39)	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21,832,083 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GTCATTGCAAGTGCCAAGGAG -3'
(R):5'- AAAACGAAGCAGGGTTTTGTTCC -3'

Sequencing Primer
(F):5'- AGAAGACACTTGGCCTGGCTG -3'
(R):5'- CACTTCAGAAGCTGCGATTG -3'

Posted On

2015-07-21