Mutagenetix > Incidental Mutation

Incidental Mutation 'R4514:F5'

332773

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000026579

Gene Name

coagulation factor V

Synonyms

Cf-5, Cf5

MMRRC Submission

041588-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4514 (G1)

Quality Score

116

Status

Not validated

Chromosome

Chromosomal Location

163979407-164047846 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to A at 163979566 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000123924 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086040] [ENSMUST00000162746]

AlphaFold

O88783

PDB Structure

Structure of the SNX17 atypical FERM domain bound to the NPxY motif of P-selectin [X-RAY DIFFRACTION]

Predicted Effect

unknown
Transcript: ENSMUST00000086040
AA Change: V12E

SMART Domains

Protein: ENSMUSP00000083204
Gene: ENSMUSG00000026579
AA Change: V12E

Domain	Start	End	E-Value	Type
low complexity region	2	14	N/A	INTRINSIC
Pfam:Cu-oxidase_3	67	196	4.4e-10	PFAM
low complexity region	282	300	N/A	INTRINSIC
Pfam:Cu-oxidase_3	397	527	1.5e-7	PFAM
low complexity region	1013	1019	N/A	INTRINSIC
low complexity region	1045	1058	N/A	INTRINSIC
low complexity region	1156	1173	N/A	INTRINSIC
low complexity region	1352	1366	N/A	INTRINSIC
low complexity region	1368	1382	N/A	INTRINSIC
low complexity region	1440	1464	N/A	INTRINSIC
Pfam:Cu-oxidase_3	1600	1714	9.1e-8	PFAM
FA58C	1865	2020	8.03e-36	SMART
FA58C	2024	2180	1.96e-30	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133341

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161020

Predicted Effect

probably benign
Transcript: ENSMUST00000161152

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162102

Predicted Effect

probably benign
Transcript: ENSMUST00000162746

SMART Domains

Protein: ENSMUSP00000123924
Gene: ENSMUSG00000026580

Domain	Start	End	E-Value	Type
CLECT	30	159	2.89e-16	SMART
EGF	162	195	1.97e-4	SMART
CCP	200	257	1.31e-14	SMART
CCP	262	319	4.02e-15	SMART
CCP	324	381	5.91e-13	SMART
CCP	386	443	1.46e-12	SMART
CCP	448	505	3.9e-13	SMART
CCP	510	567	1.95e-13	SMART
CCP	580	637	1.97e-9	SMART
CCP	642	699	3.9e-13	SMART
transmembrane domain	711	733	N/A	INTRINSIC
PDB:4GXB\|B	741	768	2e-12	PDB

Coding Region Coverage

1x: 99.4%
3x: 98.6%
10x: 96.9%
20x: 94.1%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]
PHENOTYPE: Half of mice homozygous for a null allele die at E9-E10 with defects in yolk-sac vasculature and somite formation; the remaining half develop to term but die of massive hemorrhage within hours of birth. Mice homozygous for a knock-in (F5 Leiden) allele develop strain-specific perinatal thrombosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam5	T	G	8: 25,308,152 (GRCm39)	T51P	probably damaging	Het
Akr1c20	A	C	13: 4,557,843 (GRCm39)	V201G	probably damaging	Het
Alg9	C	T	9: 50,716,654 (GRCm39)	T409M	possibly damaging	Het
Arpp21	T	A	9: 112,006,745 (GRCm39)	T155S	probably damaging	Het
Atm	T	A	9: 53,404,339 (GRCm39)	Q1334L	probably damaging	Het
Bptf	G	A	11: 106,968,518 (GRCm39)	T1055M	probably damaging	Het
Cd3g	C	A	9: 44,884,882 (GRCm39)	A121S	possibly damaging	Het
Cenpn	A	G	8: 117,660,135 (GRCm39)	Y68C	probably damaging	Het
Clock	A	G	5: 76,378,046 (GRCm39)	I618T	probably benign	Het
Cp	A	G	3: 20,042,177 (GRCm39)	M982V	probably damaging	Het
Csf3r	T	A	4: 125,933,653 (GRCm39)	S611T	possibly damaging	Het
Csn3	A	G	5: 88,077,997 (GRCm39)	T168A	unknown	Het
Cylc2	C	G	4: 51,229,651 (GRCm39)	T331R	unknown	Het
D630003M21Rik	T	C	2: 158,046,722 (GRCm39)	T752A	probably benign	Het
Defb34	A	T	8: 19,176,522 (GRCm39)	D71V	probably damaging	Het
Dync1h1	T	A	12: 110,623,573 (GRCm39)	D3615E	possibly damaging	Het
Etl4	A	T	2: 20,666,709 (GRCm39)	T167S	probably damaging	Het
Got1l1	C	T	8: 27,688,513 (GRCm39)	M279I	probably benign	Het
Grm7	A	G	6: 111,335,265 (GRCm39)	T559A	possibly damaging	Het
Ifit1	A	T	19: 34,625,913 (GRCm39)	R350*	probably null	Het
Ighv2-5	T	C	12: 113,649,216 (GRCm39)	N79S	possibly damaging	Het
Igkv17-127	A	G	6: 67,838,498 (GRCm39)	I70V	possibly damaging	Het
Itga8	T	C	2: 12,187,547 (GRCm39)	S711G	probably benign	Het
Kndc1	A	G	7: 139,490,202 (GRCm39)	T235A	probably benign	Het
Lct	T	C	1: 128,228,251 (GRCm39)	I1081V	probably benign	Het
Lrrc8b	G	T	5: 105,627,819 (GRCm39)	C55F	probably damaging	Het
Lrwd1	T	C	5: 136,160,402 (GRCm39)	T311A	probably benign	Het
Mapk14	T	C	17: 28,943,798 (GRCm39)	F129S	probably damaging	Het
Mdga2	A	G	12: 66,763,496 (GRCm39)	I200T	probably damaging	Het
Mocos	T	A	18: 24,816,269 (GRCm39)	S615R	probably damaging	Het
Myh4	T	C	11: 67,146,395 (GRCm39)	V1456A	probably benign	Het
Myh9	T	C	15: 77,648,200 (GRCm39)	I1759V	probably benign	Het
Nat8f5	G	A	6: 85,794,405 (GRCm39)	T185I	possibly damaging	Het
Nav3	T	C	10: 109,529,943 (GRCm39)	I2133V	possibly damaging	Het
Ncam2	T	A	16: 81,309,884 (GRCm39)	M458K	probably benign	Het
Nphp1	T	C	2: 127,590,007 (GRCm39)	S532G	probably benign	Het
Oplah	A	G	15: 76,182,155 (GRCm39)	L1035P	probably damaging	Het
Or4p22	T	C	2: 88,317,709 (GRCm39)	V211A	probably benign	Het
Or52h2	C	T	7: 103,838,598 (GRCm39)	R272H	probably benign	Het
Pask	T	A	1: 93,249,855 (GRCm39)	Q515L	probably benign	Het
Poglut1	A	T	16: 38,369,778 (GRCm39)	F35I	probably benign	Het
Ppp1ca	T	G	19: 4,245,054 (GRCm39)	I319S	probably benign	Het
Psg25	T	A	7: 18,263,533 (GRCm39)	R97*	probably null	Het
Sars2	T	C	7: 28,441,709 (GRCm39)		probably null	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Slc16a7	T	G	10: 125,069,308 (GRCm39)		probably null	Het
Slc7a8	C	G	14: 54,973,247 (GRCm39)	G240A	possibly damaging	Het
St6gal2	A	T	17: 55,790,018 (GRCm39)	N351Y	probably benign	Het
Susd5	T	C	9: 113,924,992 (GRCm39)	F292L	probably benign	Het
Tmco5	T	A	2: 116,710,795 (GRCm39)	D38E	probably damaging	Het
Tubgcp2	G	A	7: 139,575,984 (GRCm39)	P893L	possibly damaging	Het
Uncx	A	G	5: 139,532,522 (GRCm39)	I196V	possibly damaging	Het
Zeb1	G	A	18: 5,759,007 (GRCm39)	C138Y	probably damaging	Het
Zfp609	A	G	9: 65,610,977 (GRCm39)	I662T	possibly damaging	Het
Zfp985	G	A	4: 147,668,020 (GRCm39)	C296Y	probably damaging	Het

Other mutations in F5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00840:F5	APN	1	164,007,093 (GRCm39)	missense	probably benign	0.15
IGL00843:F5	APN	1	164,039,360 (GRCm39)	missense	probably benign	0.00
IGL00904:F5	APN	1	164,021,578 (GRCm39)	missense	probably benign
IGL00913:F5	APN	1	164,032,465 (GRCm39)	missense	probably damaging	1.00
IGL01099:F5	APN	1	164,021,903 (GRCm39)	missense	probably damaging	0.99
IGL01134:F5	APN	1	164,019,548 (GRCm39)	missense	possibly damaging	0.87
IGL01313:F5	APN	1	164,021,181 (GRCm39)	missense	probably benign	0.01
IGL01635:F5	APN	1	164,035,427 (GRCm39)	missense	probably benign	0.00
IGL01697:F5	APN	1	164,021,621 (GRCm39)	missense	probably benign	0.04
IGL01768:F5	APN	1	164,003,914 (GRCm39)	missense	probably benign	0.22
IGL01795:F5	APN	1	164,021,959 (GRCm39)	missense	probably benign	0.00
IGL01835:F5	APN	1	164,021,937 (GRCm39)	missense	probably benign	0.12
IGL01843:F5	APN	1	164,039,395 (GRCm39)	missense	probably benign	0.05
IGL01989:F5	APN	1	164,003,876 (GRCm39)	missense	probably benign	0.39
IGL02036:F5	APN	1	164,010,571 (GRCm39)	splice site	probably benign
IGL02065:F5	APN	1	164,017,695 (GRCm39)	missense	probably damaging	1.00
IGL02077:F5	APN	1	164,026,435 (GRCm39)	missense	probably damaging	1.00
IGL02139:F5	APN	1	164,020,243 (GRCm39)	missense	possibly damaging	0.89
IGL02210:F5	APN	1	164,017,710 (GRCm39)	missense	probably benign	0.00
IGL02415:F5	APN	1	164,019,498 (GRCm39)	missense	probably damaging	1.00
IGL02440:F5	APN	1	164,034,635 (GRCm39)	missense	possibly damaging	0.79
IGL02471:F5	APN	1	164,001,860 (GRCm39)	missense	probably damaging	1.00
IGL02535:F5	APN	1	164,026,302 (GRCm39)	missense	probably damaging	0.98
IGL02537:F5	APN	1	164,020,686 (GRCm39)	missense	probably benign	0.26
IGL02628:F5	APN	1	164,021,644 (GRCm39)	missense	probably damaging	0.99
IGL02638:F5	APN	1	164,012,177 (GRCm39)	critical splice donor site	probably null
IGL02824:F5	APN	1	164,021,916 (GRCm39)	missense	probably benign	0.00
IGL02977:F5	APN	1	164,021,590 (GRCm39)	missense	probably damaging	1.00
IGL03028:F5	APN	1	164,020,569 (GRCm39)	nonsense	probably null
IGL03064:F5	APN	1	164,023,163 (GRCm39)	missense	probably benign	0.04
IGL03127:F5	APN	1	164,021,107 (GRCm39)	missense	probably benign	0.45
IGL03131:F5	APN	1	163,989,388 (GRCm39)	missense	possibly damaging	0.62
IGL03348:F5	APN	1	164,021,721 (GRCm39)	missense	possibly damaging	0.49
IGL03387:F5	APN	1	164,020,801 (GRCm39)	missense	probably damaging	1.00
James_dean	UTSW	1	164,032,389 (GRCm39)	missense	probably benign	0.43
BB002:F5	UTSW	1	164,003,935 (GRCm39)	critical splice donor site	probably null
BB012:F5	UTSW	1	164,003,935 (GRCm39)	critical splice donor site	probably null
R0002:F5	UTSW	1	164,029,200 (GRCm39)	missense	probably damaging	1.00
R0095:F5	UTSW	1	164,019,537 (GRCm39)	nonsense	probably null
R0116:F5	UTSW	1	164,012,483 (GRCm39)	missense	probably benign	0.01
R0359:F5	UTSW	1	164,007,018 (GRCm39)	missense	probably damaging	1.00
R0426:F5	UTSW	1	164,010,409 (GRCm39)	missense	probably damaging	0.99
R0452:F5	UTSW	1	164,012,676 (GRCm39)	missense	probably damaging	0.99
R0457:F5	UTSW	1	164,021,769 (GRCm39)	missense	probably benign	0.00
R0520:F5	UTSW	1	164,037,156 (GRCm39)	missense	probably benign	0.15
R0522:F5	UTSW	1	164,039,332 (GRCm39)	missense	probably damaging	1.00
R0554:F5	UTSW	1	164,007,018 (GRCm39)	missense	probably damaging	1.00
R0575:F5	UTSW	1	164,003,813 (GRCm39)	missense	probably damaging	1.00
R0734:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R0739:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1062:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1063:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1149:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1149:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1150:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1151:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1152:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1221:F5	UTSW	1	163,989,368 (GRCm39)	missense	probably damaging	1.00
R1284:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1286:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1358:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1360:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1362:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1383:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1465:F5	UTSW	1	164,026,402 (GRCm39)	missense	probably benign	0.02
R1465:F5	UTSW	1	164,026,402 (GRCm39)	missense	probably benign	0.02
R1545:F5	UTSW	1	164,036,529 (GRCm39)	nonsense	probably null
R1561:F5	UTSW	1	164,014,472 (GRCm39)	nonsense	probably null
R1623:F5	UTSW	1	164,023,191 (GRCm39)	missense	probably damaging	1.00
R1662:F5	UTSW	1	164,035,457 (GRCm39)	missense	probably damaging	1.00
R1673:F5	UTSW	1	164,007,089 (GRCm39)	missense	probably damaging	1.00
R1689:F5	UTSW	1	164,026,486 (GRCm39)	missense	probably damaging	1.00
R1705:F5	UTSW	1	164,045,059 (GRCm39)	missense	possibly damaging	0.92
R1732:F5	UTSW	1	164,001,719 (GRCm39)	missense	probably damaging	1.00
R1763:F5	UTSW	1	164,020,104 (GRCm39)	missense	probably benign	0.04
R1774:F5	UTSW	1	164,020,104 (GRCm39)	missense	probably benign	0.04
R1799:F5	UTSW	1	164,021,100 (GRCm39)	missense	possibly damaging	0.58
R1800:F5	UTSW	1	164,010,403 (GRCm39)	missense	probably damaging	1.00
R1842:F5	UTSW	1	164,012,129 (GRCm39)	missense	probably damaging	0.99
R1915:F5	UTSW	1	164,010,486 (GRCm39)	missense	probably damaging	0.97
R1926:F5	UTSW	1	164,007,077 (GRCm39)	missense	probably damaging	1.00
R2025:F5	UTSW	1	164,037,044 (GRCm39)	missense	probably benign	0.05
R2198:F5	UTSW	1	164,034,603 (GRCm39)	missense	probably damaging	1.00
R2258:F5	UTSW	1	164,019,750 (GRCm39)	missense	probably damaging	1.00
R2264:F5	UTSW	1	164,021,971 (GRCm39)	missense	probably benign	0.32
R2281:F5	UTSW	1	164,023,289 (GRCm39)	missense	possibly damaging	0.80
R2407:F5	UTSW	1	164,039,441 (GRCm39)	missense	probably damaging	1.00
R2445:F5	UTSW	1	164,017,795 (GRCm39)	missense	probably damaging	1.00
R2860:F5	UTSW	1	164,012,533 (GRCm39)	missense	probably damaging	1.00
R2861:F5	UTSW	1	164,012,533 (GRCm39)	missense	probably damaging	1.00
R2862:F5	UTSW	1	164,012,533 (GRCm39)	missense	probably damaging	1.00
R2899:F5	UTSW	1	164,014,469 (GRCm39)	missense	possibly damaging	0.88
R2910:F5	UTSW	1	164,032,389 (GRCm39)	missense	probably benign	0.43
R2912:F5	UTSW	1	164,021,488 (GRCm39)	missense	probably damaging	0.98
R2996:F5	UTSW	1	164,010,486 (GRCm39)	missense	probably damaging	0.97
R3745:F5	UTSW	1	164,014,348 (GRCm39)	missense	possibly damaging	0.79
R3901:F5	UTSW	1	164,003,798 (GRCm39)	missense	probably benign	0.08
R3902:F5	UTSW	1	164,003,798 (GRCm39)	missense	probably benign	0.08
R4365:F5	UTSW	1	164,012,519 (GRCm39)	missense	probably damaging	0.98
R4448:F5	UTSW	1	164,026,468 (GRCm39)	missense	possibly damaging	0.52
R4490:F5	UTSW	1	164,044,964 (GRCm39)	missense	probably benign	0.40
R4598:F5	UTSW	1	164,032,366 (GRCm39)	missense	probably benign	0.05
R4608:F5	UTSW	1	164,036,598 (GRCm39)	missense	probably benign	0.12
R4661:F5	UTSW	1	164,012,489 (GRCm39)	missense	probably damaging	1.00
R4667:F5	UTSW	1	164,001,755 (GRCm39)	missense	probably benign	0.00
R4689:F5	UTSW	1	163,979,542 (GRCm39)	unclassified	probably benign
R4716:F5	UTSW	1	164,021,488 (GRCm39)	missense	probably damaging	0.98
R4732:F5	UTSW	1	164,009,226 (GRCm39)	missense	probably damaging	1.00
R4733:F5	UTSW	1	164,009,226 (GRCm39)	missense	probably damaging	1.00
R4854:F5	UTSW	1	164,019,715 (GRCm39)	missense	probably damaging	1.00
R4908:F5	UTSW	1	164,039,389 (GRCm39)	missense	probably damaging	1.00
R4971:F5	UTSW	1	164,021,755 (GRCm39)	missense	probably benign
R5001:F5	UTSW	1	164,023,139 (GRCm39)	missense	probably benign	0.00
R5042:F5	UTSW	1	164,047,020 (GRCm39)	missense	probably damaging	1.00
R5056:F5	UTSW	1	164,019,601 (GRCm39)	missense	possibly damaging	0.60
R5061:F5	UTSW	1	164,021,749 (GRCm39)	missense	probably benign	0.00
R5143:F5	UTSW	1	164,039,397 (GRCm39)	missense	probably damaging	0.98
R5622:F5	UTSW	1	164,020,134 (GRCm39)	missense	probably benign	0.09
R5626:F5	UTSW	1	164,036,604 (GRCm39)	missense	probably damaging	0.98
R5658:F5	UTSW	1	164,019,907 (GRCm39)	missense	probably damaging	0.96
R5702:F5	UTSW	1	164,022,116 (GRCm39)	nonsense	probably null
R5795:F5	UTSW	1	163,979,578 (GRCm39)	missense	probably benign	0.09
R5884:F5	UTSW	1	164,023,215 (GRCm39)	missense	probably benign	0.01
R6036:F5	UTSW	1	164,012,565 (GRCm39)	missense	probably damaging	0.99
R6036:F5	UTSW	1	164,012,565 (GRCm39)	missense	probably damaging	0.99
R6151:F5	UTSW	1	164,017,756 (GRCm39)	missense	probably damaging	1.00
R6151:F5	UTSW	1	164,009,204 (GRCm39)	missense	probably damaging	1.00
R6345:F5	UTSW	1	164,019,520 (GRCm39)	missense	probably benign	0.13
R6391:F5	UTSW	1	164,021,062 (GRCm39)	missense	probably damaging	0.99
R6542:F5	UTSW	1	164,022,037 (GRCm39)	missense	probably benign	0.32
R6620:F5	UTSW	1	164,014,375 (GRCm39)	missense	probably damaging	1.00
R6750:F5	UTSW	1	164,021,076 (GRCm39)	missense	possibly damaging	0.58
R6754:F5	UTSW	1	164,021,332 (GRCm39)	missense	probably damaging	1.00
R6774:F5	UTSW	1	164,014,447 (GRCm39)	missense	probably damaging	1.00
R6802:F5	UTSW	1	164,006,925 (GRCm39)	missense	probably damaging	0.98
R6810:F5	UTSW	1	164,014,471 (GRCm39)	missense	probably damaging	1.00
R6983:F5	UTSW	1	164,021,698 (GRCm39)	missense	probably damaging	1.00
R7000:F5	UTSW	1	164,007,075 (GRCm39)	missense	probably damaging	1.00
R7151:F5	UTSW	1	164,029,230 (GRCm39)	missense	probably damaging	1.00
R7193:F5	UTSW	1	164,046,966 (GRCm39)	missense	probably damaging	1.00
R7230:F5	UTSW	1	164,012,522 (GRCm39)	missense	probably benign
R7324:F5	UTSW	1	164,021,150 (GRCm39)	small deletion	probably benign
R7350:F5	UTSW	1	164,020,277 (GRCm39)	missense	probably benign	0.08
R7466:F5	UTSW	1	164,020,897 (GRCm39)	missense	possibly damaging	0.61
R7503:F5	UTSW	1	164,019,779 (GRCm39)	missense	probably damaging	1.00
R7626:F5	UTSW	1	164,014,481 (GRCm39)	missense	possibly damaging	0.95
R7742:F5	UTSW	1	164,035,453 (GRCm39)	missense	possibly damaging	0.51
R7837:F5	UTSW	1	164,014,363 (GRCm39)	missense	probably damaging	1.00
R7848:F5	UTSW	1	163,989,446 (GRCm39)	missense	possibly damaging	0.94
R7925:F5	UTSW	1	164,003,935 (GRCm39)	critical splice donor site	probably null
R8053:F5	UTSW	1	164,020,338 (GRCm39)	missense	probably benign	0.26
R8094:F5	UTSW	1	164,036,509 (GRCm39)	missense	probably benign	0.06
R8175:F5	UTSW	1	164,019,834 (GRCm39)	nonsense	probably null
R8209:F5	UTSW	1	164,021,959 (GRCm39)	missense	probably benign	0.00
R8226:F5	UTSW	1	164,021,959 (GRCm39)	missense	probably benign	0.00
R8266:F5	UTSW	1	164,012,693 (GRCm39)	critical splice donor site	probably null
R8517:F5	UTSW	1	164,003,822 (GRCm39)	missense	probably damaging	0.99
R8684:F5	UTSW	1	164,045,111 (GRCm39)	missense	probably benign	0.01
R8941:F5	UTSW	1	164,026,440 (GRCm39)	missense	probably benign	0.19
R9130:F5	UTSW	1	164,001,830 (GRCm39)	missense	probably benign	0.37
R9181:F5	UTSW	1	164,019,895 (GRCm39)	missense	probably benign	0.00
R9186:F5	UTSW	1	164,021,470 (GRCm39)	missense	probably benign
R9233:F5	UTSW	1	164,047,020 (GRCm39)	missense	probably damaging	1.00
R9314:F5	UTSW	1	164,029,146 (GRCm39)	missense	probably benign	0.01
R9631:F5	UTSW	1	164,014,423 (GRCm39)	missense	probably damaging	1.00
R9655:F5	UTSW	1	164,021,730 (GRCm39)	missense	probably benign	0.15
X0024:F5	UTSW	1	164,020,557 (GRCm39)	missense	probably damaging	1.00
Z1088:F5	UTSW	1	163,981,954 (GRCm39)	missense	probably benign	0.04
Z1176:F5	UTSW	1	164,012,085 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- TAGGGTTAGACAGCCACCACAG -3'
(R):5'- TTGCACCTGGAAAGAAAGCC -3'

Sequencing Primer
(F):5'- CAGGGGACAGGGACACTCAC -3'
(R):5'- GCTAACCATTCACATTGAAATTTCC -3'

Posted On

2015-08-18