Incidental Mutation 'R4532:Zfp655'
| ID |
333141 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp655
|
| Ensembl Gene |
ENSMUSG00000007812 |
| Gene Name |
zinc finger protein 655 |
| Synonyms |
2700038I16Rik, 9030409O18Rik |
| MMRRC Submission |
041772-MU
|
| Accession Numbers |
|
| Essential gene? |
Possibly non essential
(E-score: 0.451)
|
| Stock # |
R4532 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
5 |
| Chromosomal Location |
145168525-145184112 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 145181507 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Asparagine
at position 455
(I455N)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000128969
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000167316]
[ENSMUST00000199322]
|
| AlphaFold |
Q9CZP3 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000167316
AA Change: I455N
PolyPhen 2
Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)
|
| SMART Domains |
Protein: ENSMUSP00000128969
Gene: ENSMUSG00000007812
AA Change: I455N
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
243 |
265 |
5.29e-5 |
SMART |
|
ZnF_C2H2
|
271 |
293 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
299 |
328 |
1.38e2 |
SMART |
|
ZnF_C2H2
|
334 |
356 |
6.78e-3 |
SMART |
|
ZnF_C2H2
|
361 |
383 |
3.99e0 |
SMART |
|
ZnF_C2H2
|
411 |
433 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
439 |
461 |
1.01e-1 |
SMART |
|
low complexity region
|
463 |
475 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
495 |
517 |
6.47e1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000199322
|
| Meta Mutation Damage Score |
0.1799 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 97.0%
- 20x: 94.3%
|
| Validation Efficiency |
100% (54/54) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a zinc finger protein. The zinc finger proteins are involved in DNA binding and protein-protein interactions. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 44 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Akap9 |
T |
A |
5: 4,093,948 (GRCm39) |
F2157I |
probably damaging |
Het |
| Aoc1l1 |
A |
G |
6: 48,955,101 (GRCm39) |
E647G |
possibly damaging |
Het |
| Ap3b1 |
A |
G |
13: 94,702,243 (GRCm39) |
K1099E |
unknown |
Het |
| Arhgap42 |
T |
C |
9: 9,011,433 (GRCm39) |
D451G |
probably damaging |
Het |
| Cd19 |
C |
T |
7: 126,011,281 (GRCm39) |
C301Y |
probably damaging |
Het |
| Cdh23 |
T |
C |
10: 60,370,202 (GRCm39) |
T198A |
probably benign |
Het |
| Cdt1 |
T |
C |
8: 123,298,495 (GRCm39) |
S407P |
probably benign |
Het |
| Cyp26c1 |
A |
G |
19: 37,674,227 (GRCm39) |
T34A |
probably damaging |
Het |
| Dbh |
C |
A |
2: 27,067,343 (GRCm39) |
H409Q |
possibly damaging |
Het |
| Eif5 |
T |
A |
12: 111,506,318 (GRCm39) |
C52* |
probably null |
Het |
| Fhod3 |
A |
G |
18: 25,243,278 (GRCm39) |
Y1212C |
probably damaging |
Het |
| Ggh |
T |
A |
4: 20,046,225 (GRCm39) |
F44L |
probably benign |
Het |
| Gm12185 |
T |
C |
11: 48,798,747 (GRCm39) |
Y582C |
probably damaging |
Het |
| Gm12185 |
T |
C |
11: 48,798,921 (GRCm39) |
N524S |
possibly damaging |
Het |
| Gm21095 |
A |
G |
Y: 84,131,953 (GRCm39) |
N149S |
probably damaging |
Het |
| Gys2 |
T |
A |
6: 142,400,867 (GRCm39) |
H311L |
probably damaging |
Het |
| Hcn4 |
C |
T |
9: 58,765,081 (GRCm39) |
R558C |
unknown |
Het |
| Heatr6 |
T |
C |
11: 83,660,498 (GRCm39) |
L546P |
probably damaging |
Het |
| Lin54 |
G |
A |
5: 100,594,419 (GRCm39) |
T582I |
possibly damaging |
Het |
| Lpin1 |
C |
T |
12: 16,603,963 (GRCm39) |
G623S |
probably benign |
Het |
| Lrfn2 |
A |
G |
17: 49,377,564 (GRCm39) |
D215G |
probably damaging |
Het |
| Lrrc3c |
C |
A |
11: 98,489,859 (GRCm39) |
S72* |
probably null |
Het |
| Me3 |
G |
A |
7: 89,282,108 (GRCm39) |
|
probably benign |
Het |
| Msln |
A |
G |
17: 25,969,698 (GRCm39) |
I344T |
probably damaging |
Het |
| Oma1 |
G |
A |
4: 103,176,571 (GRCm39) |
V112I |
probably benign |
Het |
| Or5al6 |
A |
T |
2: 85,976,274 (GRCm39) |
L268Q |
possibly damaging |
Het |
| Or5p4 |
T |
C |
7: 107,680,756 (GRCm39) |
Y252H |
probably benign |
Het |
| Orc4 |
G |
A |
2: 48,827,501 (GRCm39) |
P31S |
probably benign |
Het |
| Pabir3 |
G |
A |
X: 52,382,376 (GRCm39) |
R94H |
possibly damaging |
Het |
| Pdgfra |
A |
G |
5: 75,341,744 (GRCm39) |
N659S |
probably damaging |
Het |
| Rmnd5a |
A |
T |
6: 71,376,109 (GRCm39) |
|
probably null |
Het |
| Slc12a8 |
C |
A |
16: 33,371,403 (GRCm39) |
R180S |
probably damaging |
Het |
| Slc6a15 |
G |
A |
10: 103,245,648 (GRCm39) |
V544M |
possibly damaging |
Het |
| Slco5a1 |
T |
A |
1: 12,949,447 (GRCm39) |
T648S |
probably damaging |
Het |
| Snx13 |
T |
C |
12: 35,194,219 (GRCm39) |
F921L |
probably damaging |
Het |
| Stard6 |
A |
C |
18: 70,616,605 (GRCm39) |
D88A |
probably damaging |
Het |
| Svep1 |
G |
T |
4: 58,068,886 (GRCm39) |
H2967N |
possibly damaging |
Het |
| Tcaf2 |
A |
C |
6: 42,603,371 (GRCm39) |
Y730D |
probably damaging |
Het |
| Tes |
G |
A |
6: 17,097,407 (GRCm39) |
V172M |
possibly damaging |
Het |
| Ttc3 |
T |
A |
16: 94,267,736 (GRCm39) |
|
probably benign |
Het |
| Vmn1r23 |
A |
G |
6: 57,902,914 (GRCm39) |
I288T |
probably benign |
Het |
| Vmn1r38 |
G |
T |
6: 66,754,016 (GRCm39) |
H33Q |
probably benign |
Het |
| Vmn2r75 |
G |
T |
7: 85,797,349 (GRCm39) |
C821* |
probably null |
Het |
| Zfp282 |
C |
T |
6: 47,867,567 (GRCm39) |
P248S |
probably benign |
Het |
|
| Other mutations in Zfp655 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00639:Zfp655
|
APN |
5 |
145,180,955 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01679:Zfp655
|
APN |
5 |
145,180,637 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL02379:Zfp655
|
APN |
5 |
145,180,765 (GRCm39) |
missense |
probably benign |
0.13 |
|
IGL02647:Zfp655
|
APN |
5 |
145,179,816 (GRCm39) |
missense |
probably benign |
|
|
R0104:Zfp655
|
UTSW |
5 |
145,180,825 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0104:Zfp655
|
UTSW |
5 |
145,180,825 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0270:Zfp655
|
UTSW |
5 |
145,181,267 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0608:Zfp655
|
UTSW |
5 |
145,180,867 (GRCm39) |
missense |
possibly damaging |
0.57 |
|
R1528:Zfp655
|
UTSW |
5 |
145,181,411 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R2076:Zfp655
|
UTSW |
5 |
145,181,410 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R2119:Zfp655
|
UTSW |
5 |
145,181,594 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R2375:Zfp655
|
UTSW |
5 |
145,181,206 (GRCm39) |
missense |
probably benign |
0.10 |
|
R2403:Zfp655
|
UTSW |
5 |
145,181,356 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4032:Zfp655
|
UTSW |
5 |
145,180,858 (GRCm39) |
missense |
possibly damaging |
0.50 |
|
R4880:Zfp655
|
UTSW |
5 |
145,181,168 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5484:Zfp655
|
UTSW |
5 |
145,180,445 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5529:Zfp655
|
UTSW |
5 |
145,181,546 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R6193:Zfp655
|
UTSW |
5 |
145,181,586 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6195:Zfp655
|
UTSW |
5 |
145,180,572 (GRCm39) |
missense |
possibly damaging |
0.52 |
|
R7050:Zfp655
|
UTSW |
5 |
145,181,545 (GRCm39) |
missense |
probably benign |
0.12 |
|
R7471:Zfp655
|
UTSW |
5 |
145,181,542 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
R7612:Zfp655
|
UTSW |
5 |
145,173,999 (GRCm39) |
missense |
unknown |
|
|
R7626:Zfp655
|
UTSW |
5 |
145,173,917 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7989:Zfp655
|
UTSW |
5 |
145,181,380 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8557:Zfp655
|
UTSW |
5 |
145,180,835 (GRCm39) |
missense |
probably benign |
0.37 |
|
R8805:Zfp655
|
UTSW |
5 |
145,181,290 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9602:Zfp655
|
UTSW |
5 |
145,181,473 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9664:Zfp655
|
UTSW |
5 |
145,180,442 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Zfp655
|
UTSW |
5 |
145,180,813 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- ATGATGAATGTGGGCTGGCC -3'
(R):5'- GAACTTGACTAAATGTCTCCCCAAG -3'
Sequencing Primer
(F):5'- TGGGCTGGCCTATGTTAAACAAC -3'
(R):5'- CCAAGTTCACTGCATTCATAAGG -3'
|
| Posted On |
2015-08-18 |
Incidental Mutation