Mutagenetix > Incidental Mutation

Incidental Mutation 'R4532:Vmn2r75'

333150

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r75

Ensembl Gene

ENSMUSG00000090436

Gene Name

vomeronasal 2, receptor 75

Synonyms

EG546981

MMRRC Submission

041772-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R4532 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85797250-85820932 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 85797349 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 821 (C821*)

Ref Sequence

ENSEMBL: ENSMUSP00000126973 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167830]

AlphaFold

G5E8Z7

Predicted Effect

probably null
Transcript: ENSMUST00000167830
AA Change: C821*

SMART Domains

Protein: ENSMUSP00000126973
Gene: ENSMUSG00000090436
AA Change: C821*

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	80	466	2.8e-31	PFAM
Pfam:NCD3G	510	562	4.6e-20	PFAM
Pfam:7tm_3	593	829	7.7e-51	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.3%

Validation Efficiency

100% (54/54)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap9	T	A	5: 4,093,948 (GRCm39)	F2157I	probably damaging	Het
Aoc1l1	A	G	6: 48,955,101 (GRCm39)	E647G	possibly damaging	Het
Ap3b1	A	G	13: 94,702,243 (GRCm39)	K1099E	unknown	Het
Arhgap42	T	C	9: 9,011,433 (GRCm39)	D451G	probably damaging	Het
Cd19	C	T	7: 126,011,281 (GRCm39)	C301Y	probably damaging	Het
Cdh23	T	C	10: 60,370,202 (GRCm39)	T198A	probably benign	Het
Cdt1	T	C	8: 123,298,495 (GRCm39)	S407P	probably benign	Het
Cyp26c1	A	G	19: 37,674,227 (GRCm39)	T34A	probably damaging	Het
Dbh	C	A	2: 27,067,343 (GRCm39)	H409Q	possibly damaging	Het
Eif5	T	A	12: 111,506,318 (GRCm39)	C52*	probably null	Het
Fhod3	A	G	18: 25,243,278 (GRCm39)	Y1212C	probably damaging	Het
Ggh	T	A	4: 20,046,225 (GRCm39)	F44L	probably benign	Het
Gm12185	T	C	11: 48,798,747 (GRCm39)	Y582C	probably damaging	Het
Gm12185	T	C	11: 48,798,921 (GRCm39)	N524S	possibly damaging	Het
Gm21095	A	G	Y: 84,131,953 (GRCm39)	N149S	probably damaging	Het
Gys2	T	A	6: 142,400,867 (GRCm39)	H311L	probably damaging	Het
Hcn4	C	T	9: 58,765,081 (GRCm39)	R558C	unknown	Het
Heatr6	T	C	11: 83,660,498 (GRCm39)	L546P	probably damaging	Het
Lin54	G	A	5: 100,594,419 (GRCm39)	T582I	possibly damaging	Het
Lpin1	C	T	12: 16,603,963 (GRCm39)	G623S	probably benign	Het
Lrfn2	A	G	17: 49,377,564 (GRCm39)	D215G	probably damaging	Het
Lrrc3c	C	A	11: 98,489,859 (GRCm39)	S72*	probably null	Het
Me3	G	A	7: 89,282,108 (GRCm39)		probably benign	Het
Msln	A	G	17: 25,969,698 (GRCm39)	I344T	probably damaging	Het
Oma1	G	A	4: 103,176,571 (GRCm39)	V112I	probably benign	Het
Or5al6	A	T	2: 85,976,274 (GRCm39)	L268Q	possibly damaging	Het
Or5p4	T	C	7: 107,680,756 (GRCm39)	Y252H	probably benign	Het
Orc4	G	A	2: 48,827,501 (GRCm39)	P31S	probably benign	Het
Pabir3	G	A	X: 52,382,376 (GRCm39)	R94H	possibly damaging	Het
Pdgfra	A	G	5: 75,341,744 (GRCm39)	N659S	probably damaging	Het
Rmnd5a	A	T	6: 71,376,109 (GRCm39)		probably null	Het
Slc12a8	C	A	16: 33,371,403 (GRCm39)	R180S	probably damaging	Het
Slc6a15	G	A	10: 103,245,648 (GRCm39)	V544M	possibly damaging	Het
Slco5a1	T	A	1: 12,949,447 (GRCm39)	T648S	probably damaging	Het
Snx13	T	C	12: 35,194,219 (GRCm39)	F921L	probably damaging	Het
Stard6	A	C	18: 70,616,605 (GRCm39)	D88A	probably damaging	Het
Svep1	G	T	4: 58,068,886 (GRCm39)	H2967N	possibly damaging	Het
Tcaf2	A	C	6: 42,603,371 (GRCm39)	Y730D	probably damaging	Het
Tes	G	A	6: 17,097,407 (GRCm39)	V172M	possibly damaging	Het
Ttc3	T	A	16: 94,267,736 (GRCm39)		probably benign	Het
Vmn1r23	A	G	6: 57,902,914 (GRCm39)	I288T	probably benign	Het
Vmn1r38	G	T	6: 66,754,016 (GRCm39)	H33Q	probably benign	Het
Zfp282	C	T	6: 47,867,567 (GRCm39)	P248S	probably benign	Het
Zfp655	T	A	5: 145,181,507 (GRCm39)	I455N	probably benign	Het

Other mutations in Vmn2r75

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Vmn2r75	APN	7	85,797,240 (GRCm39)	unclassified	probably benign
IGL01287:Vmn2r75	APN	7	85,797,801 (GRCm39)	missense	probably damaging	0.97
IGL01318:Vmn2r75	APN	7	85,814,774 (GRCm39)	missense	probably benign	0.06
IGL01331:Vmn2r75	APN	7	85,820,870 (GRCm39)	nonsense	probably null
IGL01406:Vmn2r75	APN	7	85,812,500 (GRCm39)	splice site	probably benign
IGL01615:Vmn2r75	APN	7	85,797,681 (GRCm39)	missense	probably benign	0.03
IGL01657:Vmn2r75	APN	7	85,813,455 (GRCm39)	missense	probably damaging	1.00
IGL02237:Vmn2r75	APN	7	85,814,786 (GRCm39)	missense	possibly damaging	0.88
IGL02275:Vmn2r75	APN	7	85,814,348 (GRCm39)	missense	probably benign	0.04
IGL02307:Vmn2r75	APN	7	85,814,974 (GRCm39)	missense	probably benign	0.00
IGL03136:Vmn2r75	APN	7	85,797,911 (GRCm39)	missense	possibly damaging	0.89
IGL03160:Vmn2r75	APN	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
IGL03244:Vmn2r75	APN	7	85,820,933 (GRCm39)	unclassified	probably benign
PIT4449001:Vmn2r75	UTSW	7	85,814,791 (GRCm39)	missense	probably damaging	1.00
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0083:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0108:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0276:Vmn2r75	UTSW	7	85,797,515 (GRCm39)	missense	probably benign	0.01
R0320:Vmn2r75	UTSW	7	85,814,288 (GRCm39)	missense	probably benign	0.36
R0471:Vmn2r75	UTSW	7	85,814,721 (GRCm39)	missense	probably benign	0.01
R0562:Vmn2r75	UTSW	7	85,797,449 (GRCm39)	nonsense	probably null
R0631:Vmn2r75	UTSW	7	85,812,478 (GRCm39)	missense	probably null	1.00
R0661:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0811:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R0812:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R0891:Vmn2r75	UTSW	7	85,813,476 (GRCm39)	missense	possibly damaging	0.81
R1340:Vmn2r75	UTSW	7	85,797,798 (GRCm39)	missense	probably damaging	0.98
R1501:Vmn2r75	UTSW	7	85,814,850 (GRCm39)	missense	possibly damaging	0.85
R1760:Vmn2r75	UTSW	7	85,798,019 (GRCm39)	missense	probably damaging	1.00
R1970:Vmn2r75	UTSW	7	85,797,470 (GRCm39)	missense	probably damaging	1.00
R2060:Vmn2r75	UTSW	7	85,814,372 (GRCm39)	missense	probably benign	0.00
R2292:Vmn2r75	UTSW	7	85,798,144 (GRCm39)	missense	probably damaging	1.00
R3688:Vmn2r75	UTSW	7	85,797,629 (GRCm39)	missense	probably damaging	0.99
R3892:Vmn2r75	UTSW	7	85,813,494 (GRCm39)	missense	probably null	1.00
R4583:Vmn2r75	UTSW	7	85,813,290 (GRCm39)	missense	possibly damaging	0.81
R4592:Vmn2r75	UTSW	7	85,815,494 (GRCm39)	missense	probably benign	0.00
R4792:Vmn2r75	UTSW	7	85,812,378 (GRCm39)	missense	possibly damaging	0.46
R4859:Vmn2r75	UTSW	7	85,797,611 (GRCm39)	missense	probably benign	0.35
R4896:Vmn2r75	UTSW	7	85,820,787 (GRCm39)	missense	probably benign	0.01
R4943:Vmn2r75	UTSW	7	85,814,705 (GRCm39)	missense	probably damaging	1.00
R4992:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably null
R5048:Vmn2r75	UTSW	7	85,814,735 (GRCm39)	missense	possibly damaging	0.66
R5063:Vmn2r75	UTSW	7	85,813,372 (GRCm39)	missense	probably benign
R5156:Vmn2r75	UTSW	7	85,813,436 (GRCm39)	missense	possibly damaging	0.51
R5243:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R5277:Vmn2r75	UTSW	7	85,815,500 (GRCm39)	missense	probably benign
R5574:Vmn2r75	UTSW	7	85,815,510 (GRCm39)	missense	probably benign	0.22
R5622:Vmn2r75	UTSW	7	85,797,702 (GRCm39)	missense	probably benign	0.15
R5680:Vmn2r75	UTSW	7	85,820,779 (GRCm39)	missense	probably benign	0.10
R5884:Vmn2r75	UTSW	7	85,814,578 (GRCm39)	missense	probably benign
R6021:Vmn2r75	UTSW	7	85,820,820 (GRCm39)	missense	probably benign	0.01
R6217:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably benign
R6242:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R6299:Vmn2r75	UTSW	7	85,814,482 (GRCm39)	missense	probably benign	0.12
R6441:Vmn2r75	UTSW	7	85,820,784 (GRCm39)	missense	probably damaging	0.99
R6495:Vmn2r75	UTSW	7	85,813,287 (GRCm39)	missense	probably benign	0.00
R6553:Vmn2r75	UTSW	7	85,813,453 (GRCm39)	missense	probably benign	0.28
R6670:Vmn2r75	UTSW	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
R7078:Vmn2r75	UTSW	7	85,815,568 (GRCm39)	missense	probably damaging	1.00
R7164:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R8411:Vmn2r75	UTSW	7	85,797,722 (GRCm39)	missense	probably damaging	1.00
R8507:Vmn2r75	UTSW	7	85,797,685 (GRCm39)	nonsense	probably null
R8559:Vmn2r75	UTSW	7	85,815,480 (GRCm39)	missense	possibly damaging	0.65
R8677:Vmn2r75	UTSW	7	85,814,410 (GRCm39)	missense	possibly damaging	0.86
R8708:Vmn2r75	UTSW	7	85,812,476 (GRCm39)	missense	probably damaging	0.99
R8778:Vmn2r75	UTSW	7	85,813,497 (GRCm39)	missense	probably benign	0.40
R8968:Vmn2r75	UTSW	7	85,820,765 (GRCm39)	nonsense	probably null
R9145:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R9316:Vmn2r75	UTSW	7	85,797,313 (GRCm39)	missense	possibly damaging	0.63
R9363:Vmn2r75	UTSW	7	85,815,423 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- CTGAAATCCAGGTCATGTGTTAG -3'
(R):5'- CGAGGAAACTGCCTGATACATTC -3'

Sequencing Primer
(F):5'- GAGTCAAAAGAAGCTTTGTGAA -3'
(R):5'- TTCAATGAAGCCAAGCTCTTGAC -3'

Posted On

2015-08-18