Mutagenetix > Incidental Mutation

Incidental Mutation 'R4551:Zfp932'

333947

Institutional Source

Beutler Lab

Gene Symbol

Zfp932

Ensembl Gene

ENSMUSG00000066613

Gene Name

zinc finger protein 932

Synonyms

2310001H12Rik

MMRRC Submission

041782-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R4551 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

110144387-110158277 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 110157505 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 401 (V401A)

Ref Sequence

ENSEMBL: ENSMUSP00000108159 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099484] [ENSMUST00000112536] [ENSMUST00000112540] [ENSMUST00000125213] [ENSMUST00000143639] [ENSMUST00000187241]

AlphaFold

E9QAG8

Predicted Effect

probably benign
Transcript: ENSMUST00000099484

SMART Domains

Protein: ENSMUSP00000097083
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112536
AA Change: V368A

PolyPhen 2 Score 0.034 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000108155
Gene: ENSMUSG00000066613
AA Change: V368A

Domain	Start	End	E-Value	Type
Blast:KRAB	1	31	5e-12	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
ZnF_C2H2	183	205	1.3e-4	SMART
ZnF_C2H2	211	233	1.1e-2	SMART
ZnF_C2H2	239	261	2.27e-4	SMART
ZnF_C2H2	267	289	1.84e-4	SMART
ZnF_C2H2	295	317	5.21e-4	SMART
ZnF_C2H2	323	345	9.73e-4	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	1.58e-3	SMART
ZnF_C2H2	407	429	5.14e-3	SMART
ZnF_C2H2	435	457	2.61e-4	SMART
ZnF_C2H2	463	485	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112540
AA Change: V401A

PolyPhen 2 Score 0.034 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000108159
Gene: ENSMUSG00000066613
AA Change: V401A

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART
ZnF_C2H2	104	126	1.1e-2	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	3.39e-3	SMART
ZnF_C2H2	188	210	1.76e-1	SMART
ZnF_C2H2	216	238	1.3e-4	SMART
ZnF_C2H2	244	266	1.1e-2	SMART
ZnF_C2H2	272	294	2.27e-4	SMART
ZnF_C2H2	300	322	1.84e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	9.73e-4	SMART
ZnF_C2H2	384	406	1.58e-3	SMART
ZnF_C2H2	412	434	1.58e-3	SMART
ZnF_C2H2	440	462	5.14e-3	SMART
ZnF_C2H2	468	490	2.61e-4	SMART
ZnF_C2H2	496	518	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125213

SMART Domains

Protein: ENSMUSP00000119843
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	2e-14	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
Pfam:zf-C2H2_6	182	191	7.3e-1	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000143639

SMART Domains

Protein: ENSMUSP00000121749
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	4	60	1.74e-14	SMART
ZnF_C2H2	103	125	1.1e-2	SMART
ZnF_C2H2	131	153	5.21e-4	SMART
ZnF_C2H2	159	181	3.39e-3	SMART
ZnF_C2H2	187	209	1.76e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000187241
AA Change: V401A

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000140446
Gene: ENSMUSG00000066613
AA Change: V401A

Domain	Start	End	E-Value	Type
KRAB	5	61	7.1e-17	SMART
ZnF_C2H2	104	126	4.8e-5	SMART
ZnF_C2H2	132	154	2.2e-6	SMART
ZnF_C2H2	160	182	1.5e-5	SMART
ZnF_C2H2	188	210	7.2e-4	SMART
ZnF_C2H2	216	238	5.4e-7	SMART
ZnF_C2H2	244	266	4.5e-5	SMART
ZnF_C2H2	272	294	9.2e-7	SMART
ZnF_C2H2	300	322	7.5e-7	SMART
ZnF_C2H2	328	350	2.1e-6	SMART
ZnF_C2H2	356	378	4e-6	SMART
ZnF_C2H2	384	406	6.5e-6	SMART
ZnF_C2H2	412	434	6.9e-6	SMART
ZnF_C2H2	440	462	2.1e-5	SMART
ZnF_C2H2	468	490	1.1e-6	SMART
ZnF_C2H2	496	518	1.1e-6	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.5%

Validation Efficiency

100% (71/71)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	T	G	11: 119,902,395 (GRCm39)	E610A	probably benign	Het
Abhd17b	T	C	19: 21,658,290 (GRCm39)	S176P	possibly damaging	Het
Adgrg7	A	G	16: 56,568,375 (GRCm39)	Y427H	probably damaging	Het
Alox15	T	A	11: 70,235,422 (GRCm39)	I647L	probably benign	Het
Ankhd1	C	A	18: 36,788,560 (GRCm39)		probably null	Het
Arid1a	A	C	4: 133,423,010 (GRCm39)		probably benign	Het
Armcx5	T	A	X: 134,647,256 (GRCm39)	V444D	probably damaging	Het
C4bp	A	G	1: 130,564,464 (GRCm39)	Y407H	possibly damaging	Het
Cog6	T	A	3: 52,905,741 (GRCm39)	E96V	probably damaging	Het
Cox20	A	C	1: 178,150,114 (GRCm39)	N96T	probably benign	Het
Cpa3	T	C	3: 20,273,934 (GRCm39)	I351V	probably benign	Het
Cpd	C	T	11: 76,702,712 (GRCm39)	G552D	probably damaging	Het
Cyld	A	G	8: 89,433,762 (GRCm39)	K184E	possibly damaging	Het
Dab2	G	A	15: 6,464,775 (GRCm39)	G324D	probably damaging	Het
Depdc1a	T	A	3: 159,228,221 (GRCm39)	D324E	probably damaging	Het
Dnah9	T	C	11: 65,732,192 (GRCm39)	E4238G	probably damaging	Het
Dync1i1	T	A	6: 5,923,206 (GRCm39)	D275E	probably benign	Het
Epgn	A	T	5: 91,175,421 (GRCm39)	K14*	probably null	Het
Farp2	T	A	1: 93,546,314 (GRCm39)	L868Q	possibly damaging	Het
Gpr45	A	G	1: 43,071,950 (GRCm39)	T198A	probably benign	Het
Grk2	C	T	19: 4,336,084 (GRCm39)	V402M	possibly damaging	Het
Gsap	A	T	5: 21,495,569 (GRCm39)	D79V	probably damaging	Het
Gtf2h3	A	G	5: 124,728,482 (GRCm39)		probably benign	Het
Hus1b	C	A	13: 31,131,059 (GRCm39)	S200I	probably damaging	Het
Hypk	A	G	2: 121,283,961 (GRCm39)		probably null	Het
Ikbke	T	C	1: 131,185,770 (GRCm39)		probably benign	Het
Kat6b	A	G	14: 21,711,516 (GRCm39)	E670G	probably damaging	Het
Kif26b	T	A	1: 178,711,600 (GRCm39)	I740N	probably damaging	Het
Lhx3	G	A	2: 26,091,202 (GRCm39)	P369L	probably damaging	Het
Man2c1	T	C	9: 57,038,445 (GRCm39)	L35P	probably damaging	Het
Mical2	T	A	7: 111,981,123 (GRCm39)	S366T	possibly damaging	Het
Mroh9	A	T	1: 162,871,662 (GRCm39)	I607N	probably damaging	Het
Mybphl	T	C	3: 108,281,479 (GRCm39)	I65T	possibly damaging	Het
Myo1g	A	G	11: 6,467,874 (GRCm39)	I187T	probably damaging	Het
Myo7b	T	C	18: 32,118,161 (GRCm39)	S822G	probably benign	Het
Nkx2-4	C	A	2: 146,926,842 (GRCm39)	A142S	probably benign	Het
Obscn	T	C	11: 59,022,472 (GRCm39)	R758G	possibly damaging	Het
Odf4	C	T	11: 68,812,866 (GRCm39)	S264N	probably benign	Het
Or12k7	G	T	2: 36,958,355 (GRCm39)	V13L	probably benign	Het
Or13a17	A	T	7: 140,271,655 (GRCm39)	Y279F	probably damaging	Het
Or56b1	A	T	7: 104,285,631 (GRCm39)	H250L	probably damaging	Het
Papolb	T	C	5: 142,514,933 (GRCm39)	I237V	probably benign	Het
Parpbp	T	A	10: 87,929,564 (GRCm39)	Q428L	possibly damaging	Het
Pcdhb16	T	C	18: 37,612,887 (GRCm39)	F616L	probably damaging	Het
Pdk3	A	T	X: 92,825,846 (GRCm39)	M253K	probably damaging	Het
Pgap2	C	A	7: 101,875,674 (GRCm39)		probably benign	Het
Pkhd1l1	C	A	15: 44,414,281 (GRCm39)	N2849K	probably damaging	Het
Pprc1	A	G	19: 46,055,664 (GRCm39)		probably benign	Het
Psma2	A	G	13: 14,791,430 (GRCm39)	Y25C	possibly damaging	Het
Ptgir	A	G	7: 16,640,794 (GRCm39)	M29V	possibly damaging	Het
Ptpn5	C	A	7: 46,740,600 (GRCm39)		probably benign	Het
Pxn	G	T	5: 115,690,779 (GRCm39)		probably benign	Het
Scd3	C	A	19: 44,203,878 (GRCm39)	A22E	probably benign	Het
Sdr42e1	T	C	8: 118,390,347 (GRCm39)	E98G	probably benign	Het
Slc14a2	A	C	18: 78,239,068 (GRCm39)	S184A	probably benign	Het
Tmem236	A	T	2: 14,223,964 (GRCm39)	Q251L	probably benign	Het
Trappc8	T	C	18: 21,007,729 (GRCm39)	T129A	probably benign	Het
Vmn1r90	A	C	7: 14,295,894 (GRCm39)	M68R	possibly damaging	Het
Vmn2r105	A	G	17: 20,446,613 (GRCm39)	V462A	probably benign	Het
Vmn2r7	T	C	3: 64,598,110 (GRCm39)	T816A	possibly damaging	Het
Vmn2r81	T	A	10: 79,104,241 (GRCm39)	I288K	possibly damaging	Het
Zfp180	A	C	7: 23,803,998 (GRCm39)	K139T	possibly damaging	Het

Other mutations in Zfp932

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0153:Zfp932	UTSW	5	110,154,834 (GRCm39)	missense	probably benign	0.27
R0268:Zfp932	UTSW	5	110,156,929 (GRCm39)	missense	probably benign	0.24
R1673:Zfp932	UTSW	5	110,156,854 (GRCm39)	missense	probably damaging	1.00
R1797:Zfp932	UTSW	5	110,144,489 (GRCm39)	start gained	probably benign
R1893:Zfp932	UTSW	5	110,157,069 (GRCm39)	missense	possibly damaging	0.71
R1942:Zfp932	UTSW	5	110,154,853 (GRCm39)	missense	probably damaging	1.00
R2073:Zfp932	UTSW	5	110,157,684 (GRCm39)	missense	possibly damaging	0.60
R2076:Zfp932	UTSW	5	110,157,334 (GRCm39)	missense	probably benign	0.01
R2329:Zfp932	UTSW	5	110,157,406 (GRCm39)	missense	probably benign	0.01
R3944:Zfp932	UTSW	5	110,157,820 (GRCm39)	missense	probably benign	0.37
R4679:Zfp932	UTSW	5	110,157,760 (GRCm39)	missense	probably damaging	1.00
R4692:Zfp932	UTSW	5	110,157,052 (GRCm39)	missense	probably damaging	1.00
R5116:Zfp932	UTSW	5	110,157,242 (GRCm39)	missense	probably benign	0.05
R5260:Zfp932	UTSW	5	110,157,501 (GRCm39)	nonsense	probably null
R5536:Zfp932	UTSW	5	110,157,713 (GRCm39)	nonsense	probably null
R6351:Zfp932	UTSW	5	110,157,209 (GRCm39)	nonsense	probably null
R6786:Zfp932	UTSW	5	110,157,606 (GRCm39)	missense	probably damaging	0.98
R6843:Zfp932	UTSW	5	110,156,581 (GRCm39)	missense	probably benign	0.02
R7437:Zfp932	UTSW	5	110,157,880 (GRCm39)	missense	probably benign	0.10
R7496:Zfp932	UTSW	5	110,156,694 (GRCm39)	missense	probably damaging	0.97
R7704:Zfp932	UTSW	5	110,157,630 (GRCm39)	missense	probably benign	0.09
R8224:Zfp932	UTSW	5	110,144,480 (GRCm39)	start gained	probably benign
R8317:Zfp932	UTSW	5	110,156,922 (GRCm39)	nonsense	probably null
R8765:Zfp932	UTSW	5	110,154,827 (GRCm39)	missense	probably benign	0.01
R8967:Zfp932	UTSW	5	110,156,883 (GRCm39)	missense	probably benign	0.00
R9083:Zfp932	UTSW	5	110,157,100 (GRCm39)	missense	probably damaging	1.00
R9142:Zfp932	UTSW	5	110,157,520 (GRCm39)	missense	probably damaging	1.00
R9511:Zfp932	UTSW	5	110,155,177 (GRCm39)	missense	possibly damaging	0.46
R9533:Zfp932	UTSW	5	110,157,787 (GRCm39)	missense	probably damaging	1.00
R9640:Zfp932	UTSW	5	110,157,064 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GTGTACTAAATCCTTTGCCTCTCAT -3'
(R):5'- GCTTTGCCACATTGCTCACA -3'

Sequencing Primer
(F):5'- ATCAGTGTGGTAAAGCCTTTGCC -3'
(R):5'- GCCACATTGCTCACATTCATAGGG -3'

Posted On

2015-08-18