Mutagenetix > Incidental Mutation

Incidental Mutation 'R4518:Or7g27'

334073

Institutional Source

Beutler Lab

Gene Symbol

Or7g27

Ensembl Gene

ENSMUSG00000061614

Gene Name

olfactory receptor family 7 subfamily G member 27

Synonyms

MOR150-1P, GA_x6K02T2PVTD-13076685-13077623, MOR150-2, Olfr1522-ps1, MOR150-1, Olfr845, MOR150-1P

MMRRC Submission

041762-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R4518 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19249755-19250696 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19250556 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 267 (S267T)

Ref Sequence

ENSEMBL: ENSMUSP00000150474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071259] [ENSMUST00000213344] [ENSMUST00000215572]

AlphaFold

Q7TRG2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000071259
AA Change: S267T

PolyPhen 2 Score 0.862 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000071239
Gene: ENSMUSG00000061614
AA Change: S267T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.1e-56	PFAM
Pfam:7tm_1	41	290	2.1e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212246
AA Change: S267T

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213344
AA Change: S267T

PolyPhen 2 Score 0.862 (Sensitivity: 0.83; Specificity: 0.93)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215572
AA Change: S267T

PolyPhen 2 Score 0.862 (Sensitivity: 0.83; Specificity: 0.93)

Meta Mutation Damage Score

0.3806

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

98% (60/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110082J24Rik	T	A	5: 30,311,006 (GRCm39)		probably null	Het
Abr	A	G	11: 76,363,344 (GRCm39)	S167P	possibly damaging	Het
Adamtsl2	T	C	2: 26,985,559 (GRCm39)	L481P	probably benign	Het
Atp8b3	C	T	10: 80,359,681 (GRCm39)	M984I	probably benign	Het
Brip1	G	A	11: 85,968,704 (GRCm39)	A827V	possibly damaging	Het
Carns1	T	G	19: 4,220,069 (GRCm39)	T389P	probably benign	Het
Ccdc88a	T	C	11: 29,432,651 (GRCm39)	I1219T	probably benign	Het
Cckar	T	C	5: 53,857,264 (GRCm39)	N311S	probably damaging	Het
Cenpc1	A	G	5: 86,195,446 (GRCm39)	S108P	possibly damaging	Het
Chpf	G	T	1: 75,451,689 (GRCm39)	S588R	probably damaging	Het
Clca3a2	T	A	3: 144,514,466 (GRCm39)	T414S	probably damaging	Het
Cntrl	A	G	2: 35,038,986 (GRCm39)	E1092G	probably damaging	Het
Crb2	C	A	2: 37,680,401 (GRCm39)	T443K	probably damaging	Het
Crppa	G	A	12: 36,523,179 (GRCm39)	V203I	possibly damaging	Het
Cwf19l1	A	G	19: 44,121,473 (GRCm39)	V25A	probably damaging	Het
Cyb561d1	T	C	3: 108,106,887 (GRCm39)	I111V	possibly damaging	Het
Dbn1	A	T	13: 55,624,042 (GRCm39)	I350N	possibly damaging	Het
Dnajb4	T	C	3: 151,890,813 (GRCm39)	I329V	probably benign	Het
Dnmt1	G	T	9: 20,823,274 (GRCm39)	D1055E	probably benign	Het
Fam168a	C	T	7: 100,483,247 (GRCm39)	A176V	probably damaging	Het
Farp2	A	G	1: 93,548,363 (GRCm39)	E1016G	probably benign	Het
Fibcd1	A	T	2: 31,707,207 (GRCm39)	V350E	probably damaging	Het
Galnt3	C	A	2: 65,923,954 (GRCm39)	R438L	probably damaging	Het
Golga4	T	A	9: 118,388,076 (GRCm39)	S1733T	probably benign	Het
Golgb1	G	A	16: 36,749,625 (GRCm39)	E3076K	probably damaging	Het
Grm7	A	G	6: 110,891,507 (GRCm39)		probably null	Het
Hcn2	T	C	10: 79,560,536 (GRCm39)	V289A	probably benign	Het
Hipk1	G	T	3: 103,657,688 (GRCm39)	H799N	probably damaging	Het
Klf14	A	G	6: 30,934,867 (GRCm39)	S256P	possibly damaging	Het
Muc6	T	A	7: 141,230,489 (GRCm39)	T1214S	probably benign	Het
Nlrp2	T	A	7: 5,328,055 (GRCm39)	D666V	possibly damaging	Het
Ntng1	A	G	3: 109,842,329 (GRCm39)	I148T	probably damaging	Het
Or4k35	T	C	2: 111,100,263 (GRCm39)	M150V	probably benign	Het
Or7d9	A	G	9: 20,197,546 (GRCm39)	I184V	probably benign	Het
Orc4	G	A	2: 48,827,501 (GRCm39)	P31S	probably benign	Het
Pabir3	G	A	X: 52,382,376 (GRCm39)	R94H	possibly damaging	Het
Pacs2	A	G	12: 113,024,289 (GRCm39)	D360G	probably benign	Het
Parp8	C	A	13: 117,032,209 (GRCm39)	L321F	possibly damaging	Het
Pdss2	T	A	10: 43,248,146 (GRCm39)	S217T	probably damaging	Het
Plekhn1	T	C	4: 156,309,988 (GRCm39)	S109G	probably damaging	Het
Prlr	C	A	15: 10,329,085 (GRCm39)	T520K	possibly damaging	Het
Prokr2	T	A	2: 132,216,012 (GRCm39)		probably null	Het
Rab3gap2	G	A	1: 184,999,265 (GRCm39)	V991I	probably benign	Het
Reln	T	C	5: 22,106,741 (GRCm39)	I3210V	probably benign	Het
Rims2	T	C	15: 39,300,922 (GRCm39)	Y218H	probably damaging	Het
Slc41a1	T	C	1: 131,766,863 (GRCm39)	V127A	probably damaging	Het
St8sia6	T	A	2: 13,797,562 (GRCm39)		probably null	Het
Tlr6	A	G	5: 65,112,247 (GRCm39)	F220S	possibly damaging	Het
Trmt1l	C	T	1: 151,324,094 (GRCm39)	Q314*	probably null	Het
Ubqlnl	C	T	7: 103,798,925 (GRCm39)	V191M	probably benign	Het
Vnn3	G	A	10: 23,743,124 (GRCm39)	V445M	possibly damaging	Het
Ypel1	A	T	16: 16,913,905 (GRCm39)	F173I	possibly damaging	Het
Zfhx4	T	C	3: 5,477,578 (GRCm39)	C3398R	probably damaging	Het
Zfyve16	T	C	13: 92,657,820 (GRCm39)	E697G	possibly damaging	Het

Other mutations in Or7g27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01546:Or7g27	APN	9	19,250,068 (GRCm39)	missense	possibly damaging	0.56
IGL01637:Or7g27	APN	9	19,250,260 (GRCm39)	missense	probably damaging	1.00
IGL01767:Or7g27	APN	9	19,250,598 (GRCm39)	missense	possibly damaging	0.54
IGL01945:Or7g27	APN	9	19,250,628 (GRCm39)	missense	probably damaging	0.98
IGL02202:Or7g27	APN	9	19,250,545 (GRCm39)	missense	probably benign	0.06
IGL02877:Or7g27	APN	9	19,250,497 (GRCm39)	missense	possibly damaging	0.86
R0466:Or7g27	UTSW	9	19,250,475 (GRCm39)	missense	probably damaging	1.00
R1521:Or7g27	UTSW	9	19,249,948 (GRCm39)	missense	probably benign	0.35
R1650:Or7g27	UTSW	9	19,249,943 (GRCm39)	missense	possibly damaging	0.49
R1766:Or7g27	UTSW	9	19,250,154 (GRCm39)	missense	probably benign	0.06
R2060:Or7g27	UTSW	9	19,250,352 (GRCm39)	missense	probably benign	0.01
R2082:Or7g27	UTSW	9	19,250,574 (GRCm39)	missense	probably benign	0.36
R2257:Or7g27	UTSW	9	19,249,789 (GRCm39)	missense	probably benign	0.01
R2892:Or7g27	UTSW	9	19,250,034 (GRCm39)	missense	probably benign	0.04
R3156:Or7g27	UTSW	9	19,250,720 (GRCm39)	splice site	probably null
R3943:Or7g27	UTSW	9	19,250,371 (GRCm39)	missense	probably benign	0.05
R4116:Or7g27	UTSW	9	19,249,940 (GRCm39)	missense	probably benign	0.39
R4814:Or7g27	UTSW	9	19,250,476 (GRCm39)	missense	probably damaging	1.00
R5339:Or7g27	UTSW	9	19,250,455 (GRCm39)	missense	possibly damaging	0.78
R6647:Or7g27	UTSW	9	19,249,925 (GRCm39)	missense	possibly damaging	0.50
R7493:Or7g27	UTSW	9	19,250,109 (GRCm39)	missense	probably damaging	0.98
R7522:Or7g27	UTSW	9	19,250,294 (GRCm39)	nonsense	probably null
R7584:Or7g27	UTSW	9	19,250,569 (GRCm39)	missense	possibly damaging	0.94
R9127:Or7g27	UTSW	9	19,250,026 (GRCm39)	missense	probably benign
R9463:Or7g27	UTSW	9	19,250,320 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- AGCTTGCCTGCTCAGATACTC -3'
(R):5'- CCTTGAAATCCAGCCACTATGG -3'

Sequencing Primer
(F):5'- ATATCTCAGCTTTCATGTTTGGTG -3'
(R):5'- GAAGCTATCCTTCCAACGT -3'

Posted On

2015-08-18