Mutagenetix > Incidental Mutation

Incidental Mutation 'R4561:Mslnl'

343114

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

041786-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4561 (G1)

Quality Score

199

Status

Not validated

Chromosome

Chromosomal Location

25955016-25967304 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25961908 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ank3	T	C	10: 69,837,848 (GRCm39)	S1601P	probably damaging	Het
Arnt	A	G	3: 95,359,924 (GRCm39)	N56D	probably damaging	Het
Atad5	A	G	11: 79,986,715 (GRCm39)	T601A	probably benign	Het
Calr4	A	G	4: 109,103,379 (GRCm39)	N163S	probably damaging	Het
Cenpc1	C	A	5: 86,195,491 (GRCm39)	A93S	probably damaging	Het
Cep135	C	T	5: 76,786,040 (GRCm39)	H1048Y	possibly damaging	Het
Ctnna2	A	G	6: 77,613,696 (GRCm39)		probably null	Het
Ddx60	T	C	8: 62,395,495 (GRCm39)	L144P	probably damaging	Het
Dera	A	T	6: 137,757,736 (GRCm39)	T96S	possibly damaging	Het
Dock9	T	A	14: 121,796,419 (GRCm39)	M1853L	probably benign	Het
Erbb4	G	A	1: 68,383,080 (GRCm39)	R306*	probably null	Het
Glyat	G	T	19: 12,628,644 (GRCm39)	L146F	possibly damaging	Het
Grk4	C	A	5: 34,852,157 (GRCm39)	Q134K	probably benign	Het
Hkdc1	T	C	10: 62,245,618 (GRCm39)	Q181R	probably benign	Het
Huwe1	A	T	X: 150,646,955 (GRCm39)	I682F	probably damaging	Het
Ipo4	C	T	14: 55,867,546 (GRCm39)		probably benign	Het
Ivl	CCTGCTGCTGCT	CCTGCTGCTGCTGCT	3: 92,479,262 (GRCm39)		probably benign	Het
Kcnd2	A	G	6: 21,216,395 (GRCm39)	Q33R	probably benign	Het
Kdm7a	C	T	6: 39,129,757 (GRCm39)	R473Q	probably damaging	Het
Klhl30	A	T	1: 91,288,753 (GRCm39)	H504L	probably damaging	Het
Map4	A	G	9: 109,881,439 (GRCm39)	Y101C	possibly damaging	Het
Mfn2	C	A	4: 147,961,492 (GRCm39)	R707L	probably damaging	Het
Myof	T	C	19: 37,911,438 (GRCm39)	N1511D	probably benign	Het
Neb	A	T	2: 52,176,167 (GRCm39)	Y1431N	probably damaging	Het
Nlrc5	A	G	8: 95,203,774 (GRCm39)	T625A	probably damaging	Het
Or5g27	G	T	2: 85,409,964 (GRCm39)	C127F	probably damaging	Het
Pax2	A	G	19: 44,824,402 (GRCm39)	Y374C	unknown	Het
Pde8a	T	A	7: 80,958,568 (GRCm39)	Y315*	probably null	Het
Pkhd1	A	T	1: 20,604,943 (GRCm39)	L1124Q	possibly damaging	Het
Ppp1r3a	A	G	6: 14,754,681 (GRCm39)	F189L	probably damaging	Het
Prex2	G	A	1: 11,254,769 (GRCm39)		probably null	Het
Robo4	CGG	CG	9: 37,322,786 (GRCm39)		probably null	Het
Slc22a22	T	A	15: 57,126,781 (GRCm39)	Q77L	probably damaging	Het
Slc24a2	A	T	4: 87,145,634 (GRCm39)	V140D	probably damaging	Het
Slc35g2	C	A	9: 100,435,287 (GRCm39)	R128L	probably damaging	Het
Slco1b2	A	G	6: 141,616,893 (GRCm39)	T409A	probably benign	Het
Spag7	T	C	11: 70,555,816 (GRCm39)	I80M	probably damaging	Het
Srgap3	A	G	6: 112,758,015 (GRCm39)	M164T	probably damaging	Het
Sspo	A	T	6: 48,452,468 (GRCm39)		probably null	Het
Tcte2	T	C	17: 13,942,864 (GRCm39)		probably benign	Het
Tmem117	A	T	15: 94,992,677 (GRCm39)	M446L	probably benign	Het
Tmtc4	T	C	14: 123,200,710 (GRCm39)	T194A	probably benign	Het
Ttc21b	T	C	2: 66,016,562 (GRCm39)	Y1269C	probably damaging	Het
Zfp236	A	T	18: 82,638,531 (GRCm39)	I1363N	probably damaging	Het
Zfp760	T	A	17: 21,942,648 (GRCm39)	S608T	probably benign	Het
Zfp947	G	T	17: 22,365,124 (GRCm39)	Y183*	probably null	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,962,641 (GRCm39)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,963,749 (GRCm39)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,965,125 (GRCm39)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,966,972 (GRCm39)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,963,077 (GRCm39)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,963,051 (GRCm39)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,962,177 (GRCm39)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,961,939 (GRCm39)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,963,491 (GRCm39)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,963,943 (GRCm39)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,957,952 (GRCm39)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,957,942 (GRCm39)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,965,139 (GRCm39)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,962,133 (GRCm39)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,956,816 (GRCm39)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,965,749 (GRCm39)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,956,876 (GRCm39)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,963,531 (GRCm39)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,965,047 (GRCm39)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,962,186 (GRCm39)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,962,184 (GRCm39)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,955,895 (GRCm39)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,962,157 (GRCm39)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,965,751 (GRCm39)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,965,962 (GRCm39)	missense	probably benign
R8735:Mslnl	UTSW	17	25,964,062 (GRCm39)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,964,047 (GRCm39)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,961,694 (GRCm39)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,961,506 (GRCm39)	intron	probably benign
RF007:Mslnl	UTSW	17	25,962,202 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CCATCAGAAGGGAGGTTTGAAC -3'
(R):5'- AGAAGCCACAGGTCTGAAGC -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- CCACAGGTCTGAAGCACTGAG -3'

Posted On

2015-09-24