Mutagenetix > Incidental Mutation

Incidental Mutation 'R4587:Vmn2r59'

344166

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r59

Ensembl Gene

ENSMUSG00000092032

Gene Name

vomeronasal 2, receptor 59

Synonyms

EG628444

MMRRC Submission

042006-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R4587 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41661216-41708405 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 41695648 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Tyrosine at position 255 (N255Y)

Ref Sequence

ENSEMBL: ENSMUSP00000131856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168489]

AlphaFold

E9PUT5

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000121359

Predicted Effect

probably benign
Transcript: ENSMUST00000168489
AA Change: N255Y

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000131856
Gene: ENSMUSG00000092032
AA Change: N255Y

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-44	PFAM
Pfam:NCD3G	514	567	4.3e-23	PFAM
Pfam:7tm_3	600	835	5.4e-53	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.8%
20x: 93.8%

Validation Efficiency

100% (47/47)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd16a	T	C	17: 35,320,063 (GRCm39)		probably null	Het
Adsl	T	C	15: 80,851,968 (GRCm39)		probably null	Het
Arhgap32	T	C	9: 32,172,241 (GRCm39)	S1674P	probably benign	Het
Cd244a	T	G	1: 171,405,447 (GRCm39)	D277E	probably benign	Het
Ceacam23	C	T	7: 17,620,149 (GRCm39)	L18F	possibly damaging	Het
Ces1a	A	G	8: 93,751,932 (GRCm39)	Y401H	probably damaging	Het
Chd9	G	T	8: 91,763,134 (GRCm39)	V2320F	possibly damaging	Het
Chrd	A	G	16: 20,557,325 (GRCm39)	E670G	possibly damaging	Het
Ckap2	A	G	8: 22,666,992 (GRCm39)	S290P	probably benign	Het
Cluap1	T	A	16: 3,751,680 (GRCm39)		probably null	Het
Col15a1	C	T	4: 47,257,184 (GRCm39)	T325M	probably damaging	Het
Cplane1	T	C	15: 8,230,636 (GRCm39)	I971T	possibly damaging	Het
Dnah5	T	C	15: 28,304,745 (GRCm39)	F1652S	probably damaging	Het
Dok6	T	C	18: 89,319,320 (GRCm39)	Q312R	probably benign	Het
Glis1	C	A	4: 107,484,740 (GRCm39)	H600N	possibly damaging	Het
Hivep1	A	G	13: 42,309,704 (GRCm39)	D648G	probably benign	Het
Kif22	A	T	7: 126,632,052 (GRCm39)		probably null	Het
Lmtk3	A	G	7: 45,443,504 (GRCm39)	D729G	possibly damaging	Het
Mapk8ip3	G	A	17: 25,123,761 (GRCm39)	P587L	probably damaging	Het
Muc4	G	C	16: 32,753,919 (GRCm38)	R1265P	probably benign	Het
Myt1l	A	G	12: 29,960,800 (GRCm39)	K1038E	unknown	Het
Nf1	T	A	11: 79,426,863 (GRCm39)		probably null	Het
Nom1	T	C	5: 29,656,163 (GRCm39)	S843P	possibly damaging	Het
Or1e33	A	G	11: 73,738,045 (GRCm39)	I302T	probably benign	Het
Pex14	A	G	4: 149,048,021 (GRCm39)		probably benign	Het
Ptcd1	T	C	5: 145,091,531 (GRCm39)	T523A	possibly damaging	Het
Rasip1	T	C	7: 45,282,159 (GRCm39)	V554A	possibly damaging	Het
Ric3	A	T	7: 108,653,570 (GRCm39)		probably null	Het
Skint4	G	A	4: 111,944,221 (GRCm39)	C11Y	probably damaging	Het
Smr2	G	A	5: 88,256,631 (GRCm39)	R103H	probably benign	Het
Sobp	T	C	10: 43,034,020 (GRCm39)	Y102C	probably damaging	Het
Taar7f	T	A	10: 23,926,473 (GRCm39)	F356I	probably damaging	Het
Tbcd	T	C	11: 121,496,097 (GRCm39)	V1044A	possibly damaging	Het
Tecpr1	C	G	5: 144,149,408 (GRCm39)	V340L	probably damaging	Het
Tle3	A	G	9: 61,281,295 (GRCm39)	I22V	probably damaging	Het
Trim30a	G	A	7: 104,084,851 (GRCm39)	R120*	probably null	Het
Trim72	A	G	7: 127,607,164 (GRCm39)	D231G	probably benign	Het
Vps13a	T	C	19: 16,617,403 (GRCm39)	T3002A	probably damaging	Het
Wnt7a	A	T	6: 91,343,324 (GRCm39)		probably null	Het
Zfp51	C	T	17: 21,685,178 (GRCm39)	Q598*	probably null	Het
Zfp617	A	T	8: 72,683,003 (GRCm39)	N51I	probably damaging	Het
Zfp977	A	T	7: 42,229,614 (GRCm39)	C304S	probably damaging	Het
Zic1	A	G	9: 91,246,875 (GRCm39)	S66P	probably damaging	Het

Other mutations in Vmn2r59

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r59	APN	7	41,661,488 (GRCm39)	missense	possibly damaging	0.91
IGL01432:Vmn2r59	APN	7	41,661,983 (GRCm39)	missense	possibly damaging	0.82
IGL02119:Vmn2r59	APN	7	41,695,593 (GRCm39)	missense	probably benign	0.36
IGL02216:Vmn2r59	APN	7	41,661,817 (GRCm39)	missense	probably damaging	1.00
IGL02327:Vmn2r59	APN	7	41,661,655 (GRCm39)	missense	probably benign
IGL03346:Vmn2r59	APN	7	41,693,253 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r59	APN	7	41,708,340 (GRCm39)	missense	probably benign	0.43
IGL03412:Vmn2r59	APN	7	41,661,862 (GRCm39)	missense	probably benign
PIT4366001:Vmn2r59	UTSW	7	41,695,205 (GRCm39)	missense	possibly damaging	0.91
R0068:Vmn2r59	UTSW	7	41,695,725 (GRCm39)	missense	probably damaging	0.99
R0094:Vmn2r59	UTSW	7	41,661,722 (GRCm39)	missense	probably benign	0.07
R0179:Vmn2r59	UTSW	7	41,696,432 (GRCm39)	nonsense	probably null
R0370:Vmn2r59	UTSW	7	41,662,150 (GRCm39)	missense	probably benign	0.23
R0412:Vmn2r59	UTSW	7	41,695,916 (GRCm39)	splice site	probably benign
R0465:Vmn2r59	UTSW	7	41,696,332 (GRCm39)	missense	probably benign
R0487:Vmn2r59	UTSW	7	41,696,528 (GRCm39)	nonsense	probably null
R0576:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R0632:Vmn2r59	UTSW	7	41,708,308 (GRCm39)	missense	probably damaging	1.00
R1356:Vmn2r59	UTSW	7	41,661,218 (GRCm39)	makesense	probably null
R1387:Vmn2r59	UTSW	7	41,695,521 (GRCm39)	missense	probably damaging	1.00
R1388:Vmn2r59	UTSW	7	41,695,133 (GRCm39)	missense	probably benign	0.01
R1435:Vmn2r59	UTSW	7	41,695,629 (GRCm39)	missense	possibly damaging	0.50
R1750:Vmn2r59	UTSW	7	41,695,251 (GRCm39)	missense	possibly damaging	0.50
R2020:Vmn2r59	UTSW	7	41,693,203 (GRCm39)	missense	probably damaging	1.00
R2249:Vmn2r59	UTSW	7	41,708,326 (GRCm39)	missense	probably benign	0.00
R2256:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2257:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2441:Vmn2r59	UTSW	7	41,695,570 (GRCm39)	missense	probably benign	0.00
R2511:Vmn2r59	UTSW	7	41,693,190 (GRCm39)	missense	probably damaging	1.00
R2860:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R2861:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R3690:Vmn2r59	UTSW	7	41,661,370 (GRCm39)	missense	possibly damaging	0.77
R3912:Vmn2r59	UTSW	7	41,695,744 (GRCm39)	missense	probably benign	0.00
R4167:Vmn2r59	UTSW	7	41,670,732 (GRCm39)	intron	probably benign
R4357:Vmn2r59	UTSW	7	41,661,644 (GRCm39)	missense	probably damaging	1.00
R4445:Vmn2r59	UTSW	7	41,691,874 (GRCm39)	missense	probably damaging	1.00
R4542:Vmn2r59	UTSW	7	41,695,497 (GRCm39)	missense	possibly damaging	0.93
R4616:Vmn2r59	UTSW	7	41,661,862 (GRCm39)	missense	probably benign
R4653:Vmn2r59	UTSW	7	41,693,228 (GRCm39)	missense	probably benign	0.19
R4703:Vmn2r59	UTSW	7	41,661,686 (GRCm39)	missense	probably benign	0.01
R4895:Vmn2r59	UTSW	7	41,695,218 (GRCm39)	missense	probably damaging	0.98
R4910:Vmn2r59	UTSW	7	41,693,077 (GRCm39)	missense	probably benign
R5045:Vmn2r59	UTSW	7	41,695,496 (GRCm39)	missense	possibly damaging	0.93
R5105:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R5153:Vmn2r59	UTSW	7	41,691,834 (GRCm39)	critical splice donor site	probably null
R5566:Vmn2r59	UTSW	7	41,696,247 (GRCm39)	missense	possibly damaging	0.92
R5586:Vmn2r59	UTSW	7	41,695,105 (GRCm39)	missense	probably benign	0.12
R5606:Vmn2r59	UTSW	7	41,695,318 (GRCm39)	missense	probably benign	0.27
R5616:Vmn2r59	UTSW	7	41,708,191 (GRCm39)	splice site	probably null
R5625:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R5696:Vmn2r59	UTSW	7	41,695,468 (GRCm39)	missense	probably benign	0.00
R5982:Vmn2r59	UTSW	7	41,695,491 (GRCm39)	missense	probably benign	0.00
R6106:Vmn2r59	UTSW	7	41,661,749 (GRCm39)	nonsense	probably null
R6196:Vmn2r59	UTSW	7	41,661,679 (GRCm39)	missense	probably benign	0.36
R6228:Vmn2r59	UTSW	7	41,691,835 (GRCm39)	critical splice donor site	probably null
R6590:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6625:Vmn2r59	UTSW	7	41,693,177 (GRCm39)	missense	probably benign	0.02
R6690:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6768:Vmn2r59	UTSW	7	41,661,392 (GRCm39)	missense	probably benign	0.17
R6830:Vmn2r59	UTSW	7	41,693,171 (GRCm39)	missense	probably benign	0.10
R6859:Vmn2r59	UTSW	7	41,693,277 (GRCm39)	missense	probably damaging	1.00
R7034:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7036:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7145:Vmn2r59	UTSW	7	41,695,188 (GRCm39)	missense	probably damaging	1.00
R7556:Vmn2r59	UTSW	7	41,695,233 (GRCm39)	missense	probably damaging	1.00
R7733:Vmn2r59	UTSW	7	41,661,443 (GRCm39)	missense	probably benign	0.17
R7770:Vmn2r59	UTSW	7	41,708,336 (GRCm39)	missense	probably damaging	1.00
R7812:Vmn2r59	UTSW	7	41,695,196 (GRCm39)	nonsense	probably null
R7867:Vmn2r59	UTSW	7	41,661,707 (GRCm39)	missense	probably damaging	1.00
R7975:Vmn2r59	UTSW	7	41,693,199 (GRCm39)	missense	probably damaging	1.00
R7999:Vmn2r59	UTSW	7	41,696,256 (GRCm39)	missense	probably damaging	1.00
R8267:Vmn2r59	UTSW	7	41,661,521 (GRCm39)	missense	probably damaging	0.97
R8367:Vmn2r59	UTSW	7	41,661,247 (GRCm39)	missense	probably benign	0.44
R9106:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R9135:Vmn2r59	UTSW	7	41,693,127 (GRCm39)	missense
R9135:Vmn2r59	UTSW	7	41,693,125 (GRCm39)	missense	probably benign	0.33
R9234:Vmn2r59	UTSW	7	41,661,907 (GRCm39)	missense	possibly damaging	0.67
R9273:Vmn2r59	UTSW	7	41,695,286 (GRCm39)	nonsense	probably null
R9432:Vmn2r59	UTSW	7	41,696,254 (GRCm39)	missense	probably damaging	1.00
R9433:Vmn2r59	UTSW	7	41,695,590 (GRCm39)	missense	probably damaging	0.99
R9616:Vmn2r59	UTSW	7	41,661,299 (GRCm39)	missense	probably damaging	1.00
R9654:Vmn2r59	UTSW	7	41,693,217 (GRCm39)	missense	probably benign	0.10
R9741:Vmn2r59	UTSW	7	41,708,209 (GRCm39)	missense	probably damaging	0.99
X0025:Vmn2r59	UTSW	7	41,695,365 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r59	UTSW	7	41,661,838 (GRCm39)	missense	possibly damaging	0.85
Z1176:Vmn2r59	UTSW	7	41,691,941 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAACCATCTCTTCACGGTGG -3'
(R):5'- GTCACTTGCCATAGTCTTGTTG -3'

Sequencing Primer
(F):5'- CTCTACCATAAAATAATCAGAGTGGG -3'
(R):5'- GTTGTTGCTTCATTTCCACTGG -3'

Posted On

2015-09-24