|Institutional Source||Beutler Lab|
|Gene Name||CD86 antigen|
|Synonyms||B70, B7.2, Ly58, Cd28l2, Ly-58, MB7-2, B7-2|
|Is this an essential gene?||Probably non essential (E-score: 0.040)|
|Stock #||R4593 (G1)|
|Chromosomal Location||36603869-36666081 bp(-) (GRCm38)|
|Type of Mutation||makesense|
|DNA Base Change (assembly)||A to G at 36606556 bp|
|Amino Acid Change||Stop codon to Arginine at position 310 (*310R)|
|Ref Sequence||ENSEMBL: ENSMUSP00000087047 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000089620]|
|Predicted Effect||probably null
AA Change: *310R
AA Change: *310R
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.6508|
|Coding Region Coverage||
|Validation Efficiency||96% (45/47)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
PHENOTYPE: Homozygous null mice on an NOD background display a phenotype similar to human Guillain-Barre Syndrome, exhibiting severe peripheral nervous system inflammation, sciatic nerve demyelination, elevated auto-antibodies to myelin protein zero, hindlimb paralysis, and weak forelimb grip. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cd86||
(F):5'- ACACTGCCACCTGTTTATGG -3'
(R):5'- AGGGTGGTTTTATGTCCAACATTAG -3'
(F):5'- CACCTGTTTATGGCTACTTTCC -3'
(R):5'- TCCTGGGGATGCAGAAAA -3'