Mutagenetix > Incidental Mutation

Incidental Mutation 'R4594:Kynu'

344244

Institutional Source

Beutler Lab

Gene Symbol

Kynu

Ensembl Gene

ENSMUSG00000026866

Gene Name

kynureninase

Synonyms

L-kynurenine hydrolase, 4432411A05Rik

MMRRC Submission

041810-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4594 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

43445341-43572734 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 43569902 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 395 (S395T)

Ref Sequence

ENSEMBL: ENSMUSP00000108445 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028223] [ENSMUST00000112826]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000028223
AA Change: S395T

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000028223
Gene: ENSMUSG00000026866
AA Change: S395T

Domain	Start	End	E-Value	Type
Pfam:Aminotran_5	70	400	3.8e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000112826
AA Change: S395T

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000108445
Gene: ENSMUSG00000026866
AA Change: S395T

Domain	Start	End	E-Value	Type
Pfam:Aminotran_5	70	405	8.4e-17	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148855

Meta Mutation Damage Score

0.0986

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

100% (66/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700109H08Rik	A	G	5: 3,625,754 (GRCm39)	T64A	probably damaging	Het
4933427I04Rik	A	T	4: 123,754,331 (GRCm39)	T82S	possibly damaging	Het
Adamts15	A	T	9: 30,832,743 (GRCm39)	I264N	probably damaging	Het
Alpk1	G	A	3: 127,477,203 (GRCm39)	A285V	probably damaging	Het
Auh	T	C	13: 53,067,002 (GRCm39)		probably benign	Het
Cacna2d3	A	G	14: 28,704,303 (GRCm39)	F826S	probably benign	Het
Ccdc54	G	T	16: 50,410,380 (GRCm39)	Y295*	probably null	Het
Ctnna3	G	A	10: 64,421,858 (GRCm39)	V551I	probably benign	Het
Diaph3	C	T	14: 87,223,473 (GRCm39)	C347Y	probably damaging	Het
Dnajb5	A	G	4: 42,950,842 (GRCm39)		probably benign	Het
Dscam	A	T	16: 96,519,196 (GRCm39)	I847K	possibly damaging	Het
Fam8a1	T	C	13: 46,824,742 (GRCm39)	F243S	probably damaging	Het
Fat2	T	C	11: 55,175,578 (GRCm39)	I1712V	possibly damaging	Het
Fgfr1	T	C	8: 26,063,852 (GRCm39)	V793A	probably damaging	Het
Got2	T	C	8: 96,598,814 (GRCm39)	E196G	probably benign	Het
Gsk3b	G	A	16: 37,991,063 (GRCm39)	C107Y	possibly damaging	Het
H2-M5	T	C	17: 37,298,697 (GRCm39)	T250A	possibly damaging	Het
Il17f	T	A	1: 20,848,026 (GRCm39)	T151S	probably damaging	Het
Ints12	A	G	3: 132,814,629 (GRCm39)	N279D	probably benign	Het
Kcnd3	C	T	3: 105,566,082 (GRCm39)	A421V	probably damaging	Het
Llgl1	C	T	11: 60,597,147 (GRCm39)	T226I	probably benign	Het
Mael	T	A	1: 166,063,056 (GRCm39)	Q132L	probably damaging	Het
Mcpt1	A	T	14: 56,256,109 (GRCm39)	R49S	probably benign	Het
Meioc	G	T	11: 102,564,992 (GRCm39)	G203C	probably damaging	Het
Mfsd4b1	A	G	10: 39,883,313 (GRCm39)	S46P	probably benign	Het
Muc6	G	T	7: 141,218,685 (GRCm39)	T1996N	possibly damaging	Het
Mx2	C	A	16: 97,348,632 (GRCm39)	Y268*	probably null	Het
Myom1	A	G	17: 71,407,069 (GRCm39)	D1064G	possibly damaging	Het
Nek11	A	G	9: 105,270,046 (GRCm39)		probably null	Het
Nfe2	A	G	15: 103,157,232 (GRCm39)	L253S	probably damaging	Het
Nup205	T	C	6: 35,173,424 (GRCm39)	I478T	probably benign	Het
Nxpe2	T	C	9: 48,230,782 (GRCm39)	D529G	probably damaging	Het
Oard1	T	C	17: 48,722,267 (GRCm39)	S88P	possibly damaging	Het
Or11g25	A	G	14: 50,723,619 (GRCm39)	R235G	probably benign	Het
Or2p2	C	T	13: 21,256,692 (GRCm39)	V260I	probably benign	Het
Or4n4	G	A	14: 50,519,140 (GRCm39)	T190I	probably benign	Het
Or51k1	T	C	7: 103,661,624 (GRCm39)	D95G	probably benign	Het
Or52e5	T	C	7: 104,718,797 (GRCm39)	V41A	probably benign	Het
Or6k6	G	T	1: 173,944,886 (GRCm39)	T232N	probably damaging	Het
Or8b1	A	C	9: 38,400,057 (GRCm39)	H244P	probably damaging	Het
Osgin1	C	T	8: 120,171,992 (GRCm39)	T262I	possibly damaging	Het
Plcb4	A	T	2: 135,844,519 (GRCm39)	M146L	probably damaging	Het
Prkdc	A	T	16: 15,585,830 (GRCm39)	E2456V	possibly damaging	Het
Ptk2	G	A	15: 73,078,045 (GRCm39)	A1004V	probably damaging	Het
Rab15	G	A	12: 76,847,445 (GRCm39)		probably benign	Het
Rad51ap2	T	C	12: 11,507,881 (GRCm39)	V601A	probably benign	Het
Rasef	T	A	4: 73,698,626 (GRCm39)	I12F	possibly damaging	Het
Rdh14	T	A	12: 10,444,567 (GRCm39)	N139K	probably damaging	Het
Rexo2	A	T	9: 48,391,717 (GRCm39)	V46E	probably damaging	Het
Rskr	T	A	11: 78,182,473 (GRCm39)	V94D	possibly damaging	Het
Slmap	A	T	14: 26,186,772 (GRCm39)	L68H	probably damaging	Het
Speer3	C	G	5: 13,846,394 (GRCm39)	A238G	possibly damaging	Het
Tmem132e	T	C	11: 82,325,894 (GRCm39)	I206T	possibly damaging	Het
Trappc8	A	T	18: 20,970,005 (GRCm39)	V995E	probably benign	Het
Vmn2r12	A	C	5: 109,234,301 (GRCm39)	I637S	probably damaging	Het
Vmn2r124	T	C	17: 18,294,231 (GRCm39)	F773L	probably damaging	Het
Vmn2r99	A	T	17: 19,613,924 (GRCm39)	D548V	probably damaging	Het
Wdr81	T	C	11: 75,336,620 (GRCm39)	N1590D	probably benign	Het
Zbtb6	A	T	2: 37,319,054 (GRCm39)	N291K	possibly damaging	Het
Zfp119a	G	A	17: 56,173,325 (GRCm39)	R173C	probably benign	Het
Zmynd15	T	C	11: 70,355,008 (GRCm39)	L335P	probably damaging	Het

Other mutations in Kynu

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01524:Kynu	APN	2	43,561,394 (GRCm39)	missense	possibly damaging	0.91
IGL01974:Kynu	APN	2	43,571,352 (GRCm39)	unclassified	probably benign
R0099:Kynu	UTSW	2	43,519,065 (GRCm39)	critical splice donor site	probably null
R0304:Kynu	UTSW	2	43,569,893 (GRCm39)	missense	probably damaging	0.99
R1681:Kynu	UTSW	2	43,569,837 (GRCm39)	missense	probably damaging	1.00
R1799:Kynu	UTSW	2	43,494,169 (GRCm39)	missense	possibly damaging	0.65
R2016:Kynu	UTSW	2	43,494,289 (GRCm39)	nonsense	probably null
R2345:Kynu	UTSW	2	43,471,397 (GRCm39)	missense	probably damaging	1.00
R3085:Kynu	UTSW	2	43,492,312 (GRCm39)	missense	probably benign	0.00
R3825:Kynu	UTSW	2	43,571,451 (GRCm39)	missense	probably benign
R4091:Kynu	UTSW	2	43,569,884 (GRCm39)	missense	possibly damaging	0.94
R4241:Kynu	UTSW	2	43,571,422 (GRCm39)	missense	probably benign	0.00
R4673:Kynu	UTSW	2	43,569,815 (GRCm39)	missense	probably damaging	1.00
R4871:Kynu	UTSW	2	43,569,830 (GRCm39)	missense	possibly damaging	0.58
R5371:Kynu	UTSW	2	43,479,406 (GRCm39)	missense	probably benign	0.00
R6272:Kynu	UTSW	2	43,525,001 (GRCm39)	missense	probably benign	0.01
R6342:Kynu	UTSW	2	43,571,463 (GRCm39)	missense	probably benign	0.09
R6866:Kynu	UTSW	2	43,453,122 (GRCm39)	nonsense	probably null
R7203:Kynu	UTSW	2	43,571,365 (GRCm39)	missense	probably damaging	1.00
R8163:Kynu	UTSW	2	43,518,966 (GRCm39)	missense	probably damaging	1.00
R9024:Kynu	UTSW	2	43,490,807 (GRCm39)	missense	possibly damaging	0.62
R9089:Kynu	UTSW	2	43,489,620 (GRCm39)	missense	probably damaging	1.00
R9303:Kynu	UTSW	2	43,569,768 (GRCm39)	missense	probably damaging	0.99
R9305:Kynu	UTSW	2	43,569,768 (GRCm39)	missense	probably damaging	0.99
R9506:Kynu	UTSW	2	43,571,414 (GRCm39)	missense	probably damaging	1.00
R9702:Kynu	UTSW	2	43,479,469 (GRCm39)	missense	probably damaging	1.00
R9759:Kynu	UTSW	2	43,569,881 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGCTTCTCTGTAAATCAGTAGCCAC -3'
(R):5'- CGCTGAGTGCTAAGTTGATGTC -3'

Sequencing Primer
(F):5'- GCCACTGCTAATCTGATTGTGAC -3'
(R):5'- GATTGGTTGACTAGAAGGATATGC -3'

Posted On

2015-09-25